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The map label for this gene is IctD

Identifier: 54020412

GI number: 54020412

Start: 271839

End: 272855

Strand: Reverse

Name: IctD

Synonym: mhp245

Alternate gene names: 54020412

Gene position: 272855-271839 (Counterclockwise)

Preceding gene: 54020413

Following gene: 54020411

Centisome position: 30.56

GC content: 33.63

Gene sequence:

>1017_bases
TTGTTTTTAATTAAAAAAAATTTAAATCTATCAATGAAAAAATTTAACAAAAAAGGAGAAATCAAACTTATGAAACCTAT
TAAAATAGCTCTAATTGGTGCTGGAAATGTCGGAAATTCCTTCCTTTATGCAGCAATGAATCAAGGACTTGCATCCGAGT
ATGGAATTATTGATATTAACCCTGATTTTGCCGATGGTAATGCTTTTGATTTTGAAGATGCCTCAGCTTCCTTGCCTTTT
CCGATTAGTGTCTCCCGTTATGAATATAAAGATCTAAAAGATGCCGATTTTATTGTAATTACAGCGGGAAGACCACAAAA
ACCAGGTGAAACTCGGCTTGAATTAGTAGCTGATAACATCCGAATTATCCGGGAAATTGCACTAAAAGTCAAAGAAAGTG
GCTTTAGTGGAATAAGTATTATTGTTGCTAATCCTGTTGATATAATTACAAGGGCTTACCGGGATGCTTCTGGATTTTCC
GATCAAAAAGTTATCGGTAGTGGAACTGTTTTAGATACAGCAAGGCTTCAATTTGCAATCGCAAAAAGAGCAAAAGTATC
GCCTAATTCGGTTCAGGCCTACGTGATGGGTGAACATGGTGATTCATCTTTTGTTGCTTATTCAAATATTAAAATTGCCG
GTGAATATTTCTGTGCTTATTCTAAACTAACCGGAATTGATAGCTCAAATTACGAAAAAGAACTTGAATATCCAGTTTCT
CGCCGGGCTTATGAAATTATTAATCGTAAAAGGGCAACATTTTATGGAATTGGTGCAGCTATTGCCAAAATAGTTTCTAA
TATTATCAAAGATACAAAAAATATTATGATTGCCGGAGCAAATTTACGAGGAGAATACGGATTTCACGGAGTAAATATCG
GAGTTCCAGTTGTTTTAGGGGCAAACGGAATTGAAAAAATTATTGAGATTAGTCTTAATGATAAAGAAAAAGAAAAATTT
GCCAAATCAGTTGCAATCATTGATAAAATTTATCAGGATGCAATTAAAAATATTTAA

Upstream 100 bases:

>100_bases
TAAAAATACGTAGTTTTTCTGAAATTGAACGAATCAAAAAATAAAAAAAATTTCATTTTTGATTCGTTTTAATTAAAAAT
AAGAAAAAAATTATCATTAC

Downstream 100 bases:

>100_bases
TTTTATCCTAAAAAATTTTTAAAATTTTTAAGGAAAAACAGTTTAAAAATCTCTCTATAAATTTAATATTTTTATCCTAT
GATTTTAAAAAAAGTACTAT

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH; Immunogenic protein p36 [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MFLIKKNLNLSMKKFNKKGEIKLMKPIKIALIGAGNVGNSFLYAAMNQGLASEYGIIDINPDFADGNAFDFEDASASLPF
PISVSRYEYKDLKDADFIVITAGRPQKPGETRLELVADNIRIIREIALKVKESGFSGISIIVANPVDIITRAYRDASGFS
DQKVIGSGTVLDTARLQFAIAKRAKVSPNSVQAYVMGEHGDSSFVAYSNIKIAGEYFCAYSKLTGIDSSNYEKELEYPVS
RRAYEIINRKRATFYGIGAAIAKIVSNIIKDTKNIMIAGANLRGEYGFHGVNIGVPVVLGANGIEKIIEISLNDKEKEKF
AKSVAIIDKIYQDAIKNI

Sequences:

>Translated_338_residues
MFLIKKNLNLSMKKFNKKGEIKLMKPIKIALIGAGNVGNSFLYAAMNQGLASEYGIIDINPDFADGNAFDFEDASASLPF
PISVSRYEYKDLKDADFIVITAGRPQKPGETRLELVADNIRIIREIALKVKESGFSGISIIVANPVDIITRAYRDASGFS
DQKVIGSGTVLDTARLQFAIAKRAKVSPNSVQAYVMGEHGDSSFVAYSNIKIAGEYFCAYSKLTGIDSSNYEKELEYPVS
RRAYEIINRKRATFYGIGAAIAKIVSNIIKDTKNIMIAGANLRGEYGFHGVNIGVPVVLGANGIEKIIEISLNDKEKEKF
AKSVAIIDKIYQDAIKNI
>Mature_338_residues
MFLIKKNLNLSMKKFNKKGEIKLMKPIKIALIGAGNVGNSFLYAAMNQGLASEYGIIDINPDFADGNAFDFEDASASLPF
PISVSRYEYKDLKDADFIVITAGRPQKPGETRLELVADNIRIIREIALKVKESGFSGISIIVANPVDIITRAYRDASGFS
DQKVIGSGTVLDTARLQFAIAKRAKVSPNSVQAYVMGEHGDSSFVAYSNIKIAGEYFCAYSKLTGIDSSNYEKELEYPVS
RRAYEIINRKRATFYGIGAAIAKIVSNIIKDTKNIMIAGANLRGEYGFHGVNIGVPVVLGANGIEKIIEISLNDKEKEKF
AKSVAIIDKIYQDAIKNI

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI5031857, Length=298, Percent_Identity=33.5570469798658, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI260099723, Length=298, Percent_Identity=33.5570469798658, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI291575128, Length=309, Percent_Identity=31.7152103559871, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI4557032, Length=309, Percent_Identity=31.7152103559871, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI15082234, Length=317, Percent_Identity=32.4921135646688, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI9257228, Length=310, Percent_Identity=31.6129032258064, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI4504973, Length=310, Percent_Identity=31.6129032258064, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI47059044, Length=308, Percent_Identity=30.8441558441558, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI221136809, Length=308, Percent_Identity=30.8441558441558, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI260099727, Length=218, Percent_Identity=34.4036697247706, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI260099725, Length=211, Percent_Identity=35.0710900473934, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI207028494, Length=185, Percent_Identity=34.5945945945946, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI103472011, Length=310, Percent_Identity=23.8709677419355, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17535107, Length=287, Percent_Identity=31.7073170731707, Blast_Score=167, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6322765, Length=230, Percent_Identity=26.9565217391304, Blast_Score=62, Evalue=9e-11,
Organism=Drosophila melanogaster, GI17136226, Length=306, Percent_Identity=32.6797385620915, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI45550422, Length=319, Percent_Identity=29.4670846394984, Blast_Score=137, Evalue=8e-33,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.27 [H]

Molecular weight: Translated: 37047; Mature: 37047

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLIKKNLNLSMKKFNKKGEIKLMKPIKIALIGAGNVGNSFLYAAMNQGLASEYGIIDIN
CEEEECCCCCCHHHCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHCCCCCCCCEEECC
PDFADGNAFDFEDASASLPFPISVSRYEYKDLKDADFIVITAGRPQKPGETRLELVADNI
CCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHH
RIIREIALKVKESGFSGISIIVANPVDIITRAYRDASGFSDQKVIGSGTVLDTARLQFAI
HHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCEEECCCCEEEHHHHHEEE
AKRAKVSPNSVQAYVMGEHGDSSFVAYSNIKIAGEYFCAYSKLTGIDSSNYEKELEYPVS
HHHCCCCCCCEEEEEEECCCCCCEEEEEEEEEEHHHHEEHHHHCCCCCCCCCHHHCCCHH
RRAYEIINRKRATFYGIGAAIAKIVSNIIKDTKNIMIAGANLRGEYGFHGVNIGVPVVLG
HHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCEEEEC
ANGIEKIIEISLNDKEKEKFAKSVAIIDKIYQDAIKNI
CCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFLIKKNLNLSMKKFNKKGEIKLMKPIKIALIGAGNVGNSFLYAAMNQGLASEYGIIDIN
CEEEECCCCCCHHHCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHCCCCCCCCEEECC
PDFADGNAFDFEDASASLPFPISVSRYEYKDLKDADFIVITAGRPQKPGETRLELVADNI
CCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHH
RIIREIALKVKESGFSGISIIVANPVDIITRAYRDASGFSDQKVIGSGTVLDTARLQFAI
HHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCEEECCCCEEEHHHHHEEE
AKRAKVSPNSVQAYVMGEHGDSSFVAYSNIKIAGEYFCAYSKLTGIDSSNYEKELEYPVS
HHHCCCCCCCEEEEEEECCCCCCEEEEEEEEEEHHHHEEHHHHCCCCCCCCCHHHCCCHH
RRAYEIINRKRATFYGIGAAIAKIVSNIIKDTKNIMIAGANLRGEYGFHGVNIGVPVVLG
HHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCEEEEC
ANGIEKIIEISLNDKEKEKFAKSVAIIDKIYQDAIKNI
CCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA