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The map label for this gene is gltX

Identifier: 54020408

GI number: 54020408

Start: 265297

End: 266700

Strand: Reverse

Name: gltX

Synonym: mhp241

Alternate gene names: 54020408

Gene position: 266700-265297 (Counterclockwise)

Preceding gene: 54020409

Following gene: 54020407

Centisome position: 29.87

GC content: 30.41

Gene sequence:

>1404_bases
ATGAAAATAAGAACTCGCTATGCTCCTTCACCGACCGGATTTTTACACATTGGTGGGGCCAGAACTGCGCTTTTTAACTA
CCTTTTTGCAAAACATCATAACGGGGATTTTATTTTACGGATCGAAGACAGTGATAATTCACGAAATATCAAAGATGGAG
AAAAGTCGCAGATCGAAAATCTTTTATGACTTGGGATAATTCCGGATGAAAAACCGGGTTCAGAAACAAAATTTGGGCCT
TATCGTCAGTCAGAAAAATTAGAACGCTACCAAAAATTAGCCCAAGAACTTATAAAAAAAGGTTTTGCTTACTATGCTTT
TGATAATCAGGAAGAGCTTGAACTTCAAAAAAAAGAACAAATTGCAAAAGGGATTTTTAGTTTCCGTTATGATCAAAACT
GACTAAAAATTTCAGATCAAGAAAAACAAAAACGCTTAAAAAACAAGCATTTTGTAATCCGATTTAAGGTTGATAAGGCC
AAAAATTTTTGCTGAAATGACCTAGTTCGCGGACAAATTTGCTTTGAAGGAAGTGCAATTAGTGACTGAGTAATTATAAA
ATCCGATGGTTTTCCTACTTATAATTTTGCGGTTGTAGTTGATGATTTTGATATGGAAATAAGTCATATTTTTAGAGGTG
AAGAACATATTTCAAATACCCCAAAACAAATTGGAATATATCAGGCTTTTAATTGAAAAACTCCTAAATTCGGGCATCTT
ACGATAATTACTGACAAAAATGGCAAAAAACTGTCAAAACGTGATAAAAGTTTGTTCCAATTTATAGAGGATTACAAAAA
TCAAGGTTATCATAGTGAGGCATTTTTTAATTTTCTAGCACTTTTAGGATGAACAAGTCCTGATTCACAAGAGTTTTTTG
ACCACAAAAGTCTAATTAAGGCGTTTGATTATAAAAGACTTTCAAAGGCGCCTTCTTATTTTGATATTGAAAAATTAAAT
TGATTTTCAAAATCATATATTTCGAAAATGCCAGTTGATAAAATTCTTGAAAATTTAGAACTCTCTGATAACCAAATTTG
AAACCAATTTTTTGTTGAGACTTTTCAAAAGAGTAGTATAAAATATGCTGACTTTTACAAAAATTTTGAATTTTTTCATA
GACCAAAACAAGAGATGGATGAAAAAATGTTAGAAATTTTTGAAAAACTCGACAAAAAACCGGTTAAAATTTTTGCCAGC
AAAATTGATTATCAAAATTGAGATTATACAAAAATTAATGATTTAATCAAAGAAATTGGACAAAAACTTGAAATTACAGG
TAAAAATTTGCTTCTTCCAATCAGACTTGCAACAACTTTTACAAACTCAGGACCAGAATTGGCAAGAGCAATTTGGCTTT
TAGGAAAAAAGATAATTGAAAAAAGACTATTAAAATGAAAGTAA

Upstream 100 bases:

>100_bases
AACAAGATTTGCTTACTGACAAAAAACGGCAAAAAAGTAAAGAATTTATAACATTTTTTAAAAATTAGTTTTTAAAAAAT
TTGACTTTGAAAGGAATTTA

Downstream 100 bases:

>100_bases
AATTTTTTTCAGGCATTAATCTAGATAGTAAAATTGAAAAAATAATTGAATTTATTAGCCCAATTACTGTCACAAAAGAA
AAAATAAATAATTCGGAAAT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MKIRTRYAPSPTGFLHIGGARTALFNYLFAKHHNGDFILRIEDSDNSRNIKDGEKSQIENLLWLGIIPDEKPGSETKFGP
YRQSEKLERYQKLAQELIKKGFAYYAFDNQEELELQKKEQIAKGIFSFRYDQNWLKISDQEKQKRLKNKHFVIRFKVDKA
KNFCWNDLVRGQICFEGSAISDWVIIKSDGFPTYNFAVVVDDFDMEISHIFRGEEHISNTPKQIGIYQAFNWKTPKFGHL
TIITDKNGKKLSKRDKSLFQFIEDYKNQGYHSEAFFNFLALLGWTSPDSQEFFDHKSLIKAFDYKRLSKAPSYFDIEKLN
WFSKSYISKMPVDKILENLELSDNQIWNQFFVETFQKSSIKYADFYKNFEFFHRPKQEMDEKMLEIFEKLDKKPVKIFAS
KIDYQNWDYTKINDLIKEIGQKLEITGKNLLLPIRLATTFTNSGPELARAIWLLGKKIIEKRLLKWK

Sequences:

>Translated_467_residues
MKIRTRYAPSPTGFLHIGGARTALFNYLFAKHHNGDFILRIEDSDNSRNIKDGEKSQIENLL*LGIIPDEKPGSETKFGP
YRQSEKLERYQKLAQELIKKGFAYYAFDNQEELELQKKEQIAKGIFSFRYDQN*LKISDQEKQKRLKNKHFVIRFKVDKA
KNFC*NDLVRGQICFEGSAISD*VIIKSDGFPTYNFAVVVDDFDMEISHIFRGEEHISNTPKQIGIYQAFN*KTPKFGHL
TIITDKNGKKLSKRDKSLFQFIEDYKNQGYHSEAFFNFLALLG*TSPDSQEFFDHKSLIKAFDYKRLSKAPSYFDIEKLN
*FSKSYISKMPVDKILENLELSDNQI*NQFFVETFQKSSIKYADFYKNFEFFHRPKQEMDEKMLEIFEKLDKKPVKIFAS
KIDYQN*DYTKINDLIKEIGQKLEITGKNLLLPIRLATTFTNSGPELARAIWLLGKKIIEKRLLK*K
>Mature_467_residues
MKIRTRYAPSPTGFLHIGGARTALFNYLFAKHHNGDFILRIEDSDNSRNIKDGEKSQIENLL*LGIIPDEKPGSETKFGP
YRQSEKLERYQKLAQELIKKGFAYYAFDNQEELELQKKEQIAKGIFSFRYDQN*LKISDQEKQKRLKNKHFVIRFKVDKA
KNFC*NDLVRGQICFEGSAISD*VIIKSDGFPTYNFAVVVDDFDMEISHIFRGEEHISNTPKQIGIYQAFN*KTPKFGHL
TIITDKNGKKLSKRDKSLFQFIEDYKNQGYHSEAFFNFLALLG*TSPDSQEFFDHKSLIKAFDYKRLSKAPSYFDIEKLN
*FSKSYISKMPVDKILENLELSDNQI*NQFFVETFQKSSIKYADFYKNFEFFHRPKQEMDEKMLEIFEKLDKKPVKIFAS
KIDYQN*DYTKINDLIKEIGQKLEITGKNLLLPIRLATTFTNSGPELARAIWLLGKKIIEKRLLK*K

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=491, Percent_Identity=31.7718940936864, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI62241042, Length=214, Percent_Identity=25.2336448598131, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788743, Length=469, Percent_Identity=33.4754797441365, Blast_Score=265, Evalue=6e-72,
Organism=Escherichia coli, GI226510925, Length=253, Percent_Identity=30.8300395256917, Blast_Score=99, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71993394, Length=320, Percent_Identity=42.8125, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17511007, Length=214, Percent_Identity=24.2990654205607, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324540, Length=493, Percent_Identity=32.4543610547667, Blast_Score=192, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6321192, Length=233, Percent_Identity=28.3261802575107, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24665321, Length=491, Percent_Identity=30.9572301425662, Blast_Score=210, Evalue=1e-54,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53241; Mature: 53241

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRTRYAPSPTGFLHIGGARTALFNYLFAKHHNGDFILRIEDSDNSRNIKDGEKSQIEN
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCHHHHHH
LLLGIIPDEKPGSETKFGPYRQSEKLERYQKLAQELIKKGFAYYAFDNQEELELQKKEQI
HHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHH
AKGIFSFRYDQNLKISDQEKQKRLKNKHFVIRFKVDKAKNFCNDLVRGQICFEGSAISDV
HHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEEEEEHHHHHHHHHHHCCEEEECCCCCCEE
IIKSDGFPTYNFAVVVDDFDMEISHIFRGEEHISNTPKQIGIYQAFNKTPKFGHLTIITD
EEECCCCCCEEEEEEEECCCCHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEC
KNGKKLSKRDKSLFQFIEDYKNQGYHSEAFFNFLALLGTSPDSQEFFDHKSLIKAFDYKR
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LSKAPSYFDIEKLNFSKSYISKMPVDKILENLELSDNQINQFFVETFQKSSIKYADFYKN
HHCCCCCEEHHHCCCCHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHC
FEFFHRPKQEMDEKMLEIFEKLDKKPVKIFASKIDYQNDYTKINDLIKEIGQKLEITGKN
HHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCC
LLLPIRLATTFTNSGPELARAIWLLGKKIIEKRLLKK
EEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIRTRYAPSPTGFLHIGGARTALFNYLFAKHHNGDFILRIEDSDNSRNIKDGEKSQIEN
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCHHHHHH
LLLGIIPDEKPGSETKFGPYRQSEKLERYQKLAQELIKKGFAYYAFDNQEELELQKKEQI
HHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHH
AKGIFSFRYDQNLKISDQEKQKRLKNKHFVIRFKVDKAKNFCNDLVRGQICFEGSAISDV
HHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEEEEEHHHHHHHHHHHCCEEEECCCCCCEE
IIKSDGFPTYNFAVVVDDFDMEISHIFRGEEHISNTPKQIGIYQAFNKTPKFGHLTIITD
EEECCCCCCEEEEEEEECCCCHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEC
KNGKKLSKRDKSLFQFIEDYKNQGYHSEAFFNFLALLGTSPDSQEFFDHKSLIKAFDYKR
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LSKAPSYFDIEKLNFSKSYISKMPVDKILENLELSDNQINQFFVETFQKSSIKYADFYKN
HHCCCCCEEHHHCCCCHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHC
FEFFHRPKQEMDEKMLEIFEKLDKKPVKIFASKIDYQNDYTKINDLIKEIGQKLEITGKN
HHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCC
LLLPIRLATTFTNSGPELARAIWLLGKKIIEKRLLKK
EEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA