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The map label for this gene is yisS [H]

Identifier: 54020362

GI number: 54020362

Start: 171649

End: 172692

Strand: Reverse

Name: yisS [H]

Synonym: mhp144

Alternate gene names: 54020362

Gene position: 172692-171649 (Counterclockwise)

Preceding gene: 54020363

Following gene: 54020361

Centisome position: 19.34

GC content: 31.99

Gene sequence:

>1044_bases
ATGAAAAAAGCAAAAATTAAAGTCGGGATTTTAGGACTAGGACGAATGGGTCTAAGCCATGCTGAAAACCTTTTAGATAA
AATCCAAAATGCTGAATTAATCGCACTTTGTTCCCTTGATGAGACAGCAAAAAATTATGCTAAATTATGAAATATTCCTT
TTTGGTATAATTCTTATGAAAAAATGCTTGAAAATCAAGAAATTGAGGCTGTTATTATTGTAAGTCCTACCCCTTATCAT
CCCCAGAATATTCTTGATGCCCTTAGAGCAAAAAAACATGTTTTTTGCGAAAAACCATTAGGCACTAATCTTGAAGAAAT
TTATAACTTGGTAAAATTAGCAAAAAACTATCAAAATCAAGTAATTCAAATCGGCTTTATGCGTCGTTATGATAAAGATT
TTCACTATGCAAAACAATTAGTAGACCAAAATCAGATCGGTAAAGTTTTTTATATAAGAACTCTTTCCCAAGATCCAGAA
AAAGATATTAAAAATATGTTTTATTTTGGACCAACCTCTGGTGGTCAATTTATTGATGTAGGCGCTCATGATCTTGATTT
GATGATTTGGTATTTAAATTCTTATCCAAAACGAGTTTGATCACTTGGTGATGCTTATAAATATCAAGAATTTCGCACTT
GAAATGATGGCGATGTTGTTGCGGCCATGTTTGAATTTCATAATGGATCAATTGGAATAATGACAGCAAGCCGGATTGGC
CCTCAAGGTTATCAGGCCCAAGCGGAACTAATTGGAACAACTGGCCATCTAAATATTGGCCTAATTGATGCAAAAAATAA
CGTAATTAGTCTAAATTCTGCTGGTGCAGTTCATAATTACCACCGGAATTTTAACTCCCGTTTTAAGGATGCTTATATTG
CTGAACTAAATGATTTTGTTAATAAAATCATTGAAAAAAATTATGATAAAACGCAACTAGAGGAGGCTTTAGTAAATATA
ATTGCACTTAAAAAAGCACAAGAATCATTTCAAGATAAGCAAATTAAGGAAATAAATTATCCAGACTGGCTTGAAATTAA
ATAA

Upstream 100 bases:

>100_bases
CTTTTCTGGAAAGGCCCAGATGGAAAAGGTGAAAAACTTCAAGCCGATGAAAATGGGATACTCCAAAAGGTAAATTAATC
TATAAAAACAAGGATAATTT

Downstream 100 bases:

>100_bases
TTAAAAAAATAAAAATAACAAAAAATTAGAAGTTAAATACTTCTAATTTTTTTTATATTTTATCCAAATTTTTTCTTTAA
TTTTAATTAAAATTTAAGAA

Product: putative dehydrogenase

Products: 2,4,6/3,5-pentahydroxycyclohexanone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKKAKIKVGILGLGRMGLSHAENLLDKIQNAELIALCSLDETAKNYAKLWNIPFWYNSYEKMLENQEIEAVIIVSPTPYH
PQNILDALRAKKHVFCEKPLGTNLEEIYNLVKLAKNYQNQVIQIGFMRRYDKDFHYAKQLVDQNQIGKVFYIRTLSQDPE
KDIKNMFYFGPTSGGQFIDVGAHDLDLMIWYLNSYPKRVWSLGDAYKYQEFRTWNDGDVVAAMFEFHNGSIGIMTASRIG
PQGYQAQAELIGTTGHLNIGLIDAKNNVISLNSAGAVHNYHRNFNSRFKDAYIAELNDFVNKIIEKNYDKTQLEEALVNI
IALKKAQESFQDKQIKEINYPDWLEIK

Sequences:

>Translated_347_residues
MKKAKIKVGILGLGRMGLSHAENLLDKIQNAELIALCSLDETAKNYAKL*NIPFWYNSYEKMLENQEIEAVIIVSPTPYH
PQNILDALRAKKHVFCEKPLGTNLEEIYNLVKLAKNYQNQVIQIGFMRRYDKDFHYAKQLVDQNQIGKVFYIRTLSQDPE
KDIKNMFYFGPTSGGQFIDVGAHDLDLMIWYLNSYPKRV*SLGDAYKYQEFRT*NDGDVVAAMFEFHNGSIGIMTASRIG
PQGYQAQAELIGTTGHLNIGLIDAKNNVISLNSAGAVHNYHRNFNSRFKDAYIAELNDFVNKIIEKNYDKTQLEEALVNI
IALKKAQESFQDKQIKEINYPDWLEIK
>Mature_347_residues
MKKAKIKVGILGLGRMGLSHAENLLDKIQNAELIALCSLDETAKNYAKL*NIPFWYNSYEKMLENQEIEAVIIVSPTPYH
PQNILDALRAKKHVFCEKPLGTNLEEIYNLVKLAKNYQNQVIQIGFMRRYDKDFHYAKQLVDQNQIGKVFYIRTLSQDPE
KDIKNMFYFGPTSGGQFIDVGAHDLDLMIWYLNSYPKRV*SLGDAYKYQEFRT*NDGDVVAAMFEFHNGSIGIMTASRIG
PQGYQAQAELIGTTGHLNIGLIDAKNNVISLNSAGAVHNYHRNFNSRFKDAYIAELNDFVNKIIEKNYDKTQLEEALVNI
IALKKAQESFQDKQIKEINYPDWLEIK

Specific function: Unknown

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI87082405, Length=231, Percent_Identity=23.8095238095238, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: 1.1.1.18

Molecular weight: Translated: 39333; Mature: 39333

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAKIKVGILGLGRMGLSHAENLLDKIQNAELIALCSLDETAKNYAKLNIPFWYNSYEK
CCCCEEEEEEEEECHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCEEEECCEEECHHHH
MLENQEIEAVIIVSPTPYHPQNILDALRAKKHVFCEKPLGTNLEEIYNLVKLAKNYQNQV
HHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHE
IQIGFMRRYDKDFHYAKQLVDQNQIGKVFYIRTLSQDPEKDIKNMFYFGPTSGGQFIDVG
EEEHHHHHHCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHEEEECCCCCCCEEEEC
AHDLDLMIWYLNSYPKRVSLGDAYKYQEFRTNDGDVVAAMFEFHNGSIGIMTASRIGPQG
CCCCEEEEEEECCCCCEEECCCCHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCC
YQAQAELIGTTGHLNIGLIDAKNNVISLNSAGAVHNYHRNFNSRFKDAYIAELNDFVNKI
CCCEEEEEECCCCEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEKNYDKTQLEEALVNIIALKKAQESFQDKQIKEINYPDWLEIK
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCEEEEC
>Mature Secondary Structure
MKKAKIKVGILGLGRMGLSHAENLLDKIQNAELIALCSLDETAKNYAKLNIPFWYNSYEK
CCCCEEEEEEEEECHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCEEEECCEEECHHHH
MLENQEIEAVIIVSPTPYHPQNILDALRAKKHVFCEKPLGTNLEEIYNLVKLAKNYQNQV
HHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHE
IQIGFMRRYDKDFHYAKQLVDQNQIGKVFYIRTLSQDPEKDIKNMFYFGPTSGGQFIDVG
EEEHHHHHHCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHEEEECCCCCCCEEEEC
AHDLDLMIWYLNSYPKRVSLGDAYKYQEFRTNDGDVVAAMFEFHNGSIGIMTASRIGPQG
CCCCEEEEEEECCCCCEEECCCCHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCC
YQAQAELIGTTGHLNIGLIDAKNNVISLNSAGAVHNYHRNFNSRFKDAYIAELNDFVNKI
CCCEEEEEECCCCEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEKNYDKTQLEEALVNIIALKKAQESFQDKQIKEINYPDWLEIK
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: myo-inositol; NAD+

Specific reaction: myo-inositol + NAD+ = 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377; 8407808 [H]