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The map label for this gene is tmk

Identifier: 54020350

GI number: 54020350

Start: 141663

End: 142298

Strand: Reverse

Name: tmk

Synonym: mhp120

Alternate gene names: 54020350

Gene position: 142298-141663 (Counterclockwise)

Preceding gene: 54020351

Following gene: 54020349

Centisome position: 15.94

GC content: 30.97

Gene sequence:

>636_bases
ATGTTTATTAGTTTTGAAGGAATTGATGCAAGCGGAAAATCAACCGTGATGGACTTATTTGCAAAGTATTTAAAAATCAA
ATTTCCCGAAAAAGAGATTGTTACTACTTTTGAACCAGGAGGCAAAAATTTAAAAGAGGCGCTGCAGATCCGTGAATTTT
TACTAAGTAAGAATAATCAAATCAGCCCTTATGTAGAAATGCTTTTATTTGCAACCGCAAGACGAATTCATCTAGAAAGA
CTAATTTGGCCCGCACTAAAAGCAGGAAAAATTGTTCTTTGTGATCGCTATATTGATTCCTCGATTGCCTATCAAGGTTT
TGGAAATGGTCTTGATATTGATTTAGTTACTAGTTTAAATAGTTTGATATCTGAGAATACTTTTCCTGATTTGACAATTT
TTTTAGATATTAAAATTTCAAAAGCCTTTGAAAGAATGGGCATTTTTCGCGATCATAATCGTGATCGCCTGGAGAATAGA
GGTGTTGAATTCTATGAAAAGGTGATTAATGGTTATGAATTTTTGGCTAAAAAAAATAAAAATTTTTTTAAAATTGATGG
AAATGGTACTTATGATGAAGTTTTAGACCTCATAATTGATTTTTTTGAGAAATATTATGCAAGCTGACCAAAATAA

Upstream 100 bases:

>100_bases
GGCTTGCAACTGGAATTCCATTTGGAGCAATAATTGATTATATCGATAATATCACCTTAGATCAAGCGCTTAAAAATCGA
CAAGAAATGGAAAAATAAAT

Downstream 100 bases:

>100_bases
TTGAAGTAGTTTTCTTTATAATCTTGATAAAAACGGTAAAATTCCTCATGCCATTTTACTAATTTCAAATTATCCTAACT
TATTAGATAGTAAAATTAAC

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MFISFEGIDASGKSTVMDLFAKYLKIKFPEKEIVTTFEPGGKNLKEALQIREFLLSKNNQISPYVEMLLFATARRIHLER
LIWPALKAGKIVLCDRYIDSSIAYQGFGNGLDIDLVTSLNSLISENTFPDLTIFLDIKISKAFERMGIFRDHNRDRLENR
GVEFYEKVINGYEFLAKKNKNFFKIDGNGTYDEVLDLIIDFFEKYYASWPK

Sequences:

>Translated_211_residues
MFISFEGIDASGKSTVMDLFAKYLKIKFPEKEIVTTFEPGGKNLKEALQIREFLLSKNNQISPYVEMLLFATARRIHLER
LIWPALKAGKIVLCDRYIDSSIAYQGFGNGLDIDLVTSLNSLISENTFPDLTIFLDIKISKAFERMGIFRDHNRDRLENR
GVEFYEKVINGYEFLAKKNKNFFKIDGNGTYDEVLDLIIDFFEKYYAS*PK
>Mature_211_residues
MFISFEGIDASGKSTVMDLFAKYLKIKFPEKEIVTTFEPGGKNLKEALQIREFLLSKNNQISPYVEMLLFATARRIHLER
LIWPALKAGKIVLCDRYIDSSIAYQGFGNGLDIDLVTSLNSLISENTFPDLTIFLDIKISKAFERMGIFRDHNRDRLENR
GVEFYEKVINGYEFLAKKNKNFFKIDGNGTYDEVLDLIIDFFEKYYAS*PK

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis [H]

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787340, Length=204, Percent_Identity=31.3725490196078, Blast_Score=94, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018095
- InterPro:   IPR018094 [H]

Pfam domain/function: PF02223 Thymidylate_kin [H]

EC number: =2.7.4.9 [H]

Molecular weight: Translated: 24253; Mature: 24253

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFISFEGIDASGKSTVMDLFAKYLKIKFPEKEIVTTFEPGGKNLKEALQIREFLLSKNNQ
CEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHEEEEECCCCCCHHHHHHHHHHHHCCCCC
ISPYVEMLLFATARRIHLERLIWPALKAGKIVLCDRYIDSSIAYQGFGNGLDIDLVTSLN
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHCCHHHHCCCCCCCCHHHHHHHH
SLISENTFPDLTIFLDIKISKAFERMGIFRDHNRDRLENRGVEFYEKVINGYEFLAKKNK
HHHHCCCCCCEEEEEEEHHHHHHHHHCCHHCCCHHHHHHCCHHHHHHHHHHHHHHHHCCC
NFFKIDGNGTYDEVLDLIIDFFEKYYASPK
CEEEECCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFISFEGIDASGKSTVMDLFAKYLKIKFPEKEIVTTFEPGGKNLKEALQIREFLLSKNNQ
CEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHEEEEECCCCCCHHHHHHHHHHHHCCCCC
ISPYVEMLLFATARRIHLERLIWPALKAGKIVLCDRYIDSSIAYQGFGNGLDIDLVTSLN
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHCCHHHHCCCCCCCCHHHHHHHH
SLISENTFPDLTIFLDIKISKAFERMGIFRDHNRDRLENRGVEFYEKVINGYEFLAKKNK
HHHHCCCCCCEEEEEEEHHHHHHHHHCCHHCCCHHHHHHCCHHHHHHHHHHHHHHHHCCC
NFFKIDGNGTYDEVLDLIIDFFEKYYASPK
CEEEECCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA