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Identifier: 54020343

GI number: 54020343

Start: 134747

End: 136825

Strand: Reverse

Name: lig

Synonym: mhp113

Alternate gene names: 54020343

Gene position: 136825-134747 (Counterclockwise)

Preceding gene: 54020344

Following gene: 54020342

Centisome position: 15.33

GC content: 28.38

Gene sequence:

>2079_bases
ATGAAAAAAAACAGTCAAATTCGCGCGAAAATTATTGAACTAAGAGAAAAAATTGAAAAATGAAATCACCATTATTACCA
ATTACAGAACCCACTTGTTGATGATTTAGTATATGATAAAACTTTAAGAGAACTAGAAAAATTAGAACGCGAAAATTTTT
TCTTATTTAGTCTTGAAGAACTAAATCAATCACCAAGTCAAAAAGTTGGATCAAAAATTACCTCAAAATTTGAAAAAGTT
GCTCATTCAAGTCCGATGTTATCGCTTAATAAGGCTTATTCAAATGAGGAATTGGAAAAATGAGCAAAAAAAGCTAGAGA
AATTCTTAAAACAGTTACATTTTTTGTTGAGCCTAAAATTGATGGGATTGCCCTATCACTTTTTTACCAAAATGGCAATT
TGATTAAGGCATTAACACGCGGCGATGGAGTTTTTGGGGAAAATGTTTTAGTTAATGCACTTAAAATTAATGATGAATTT
ATTCCGAAAAAAATTAATTATCTTGAAGATTTAGAAGTCCGTGGGGAAATTTATATTGATAATTCCACATTTGCAAGTTT
ACAACTTGAGACAAAAAAATTTAAAAACCCCCGTAATGCTGCAAGCGGAATTCTGCGGCGTTATAAAAATCACAAGCCAA
AAATTGATGCAAAAAGTATTAAATTTCTTGAAGAAGAATCCGATTTTAGATATTTAAAAAGTTTTTTTTATACTTTAGTA
AACCCTGAAAAACATAAGATAAATTCCCAATTTGATAGCACTAATTTTCTAAGAAACTTAGGTTTTCAAGTAAATCCTTT
CCAAAAAAAGTGCTCTGATTTAAAAGATGTTTTTAATTTTATTAGCATAATAAAGCAAAAAAGAGATTTCCTAAATTATA
ATATTGATGGTGTTGTAGTTAAAGTTAATGAATTTTCTATCTATGAAAAATTAGGCTCAACTTCTAAATTTCCTCATAGC
GCAATTGCTTTTAAATTCGAAGACGATATTGCAAAAACTAAGCTTTTAGCGATTTTTGCAACAATTGGAAGAACAGGAAA
AGTTACTTATAATGCAAAAATTGAGCCAGTAACACTGGCTGGAAGTAAAATTTCATCAGCAATTTTACCAAATTATTCTT
ATATTGAAAATTTAAAATTGAATTTAAATACAGAAGTTTATATAAAAAAAGCCGGCGAAATAATCCCACAAATCATTGGT
AGCGTTCATAATTATCCAAAAACTAATTTTTCAATAGTAAAAAATTGTCCAAAATGTAATTCAGAATTAGTCAATAGTGA
ATCTGGACTTGATCAGTTTTGTCAAAATCAATTTTGTCCTGAAATTATTTTGCAAAAAATAGTGCATTTTTGTTCAAAAA
ATGCTTTAAATATTGAGTCCTTAGCCCAAAAAAGAATTGAAAAATTCCTTGAAAAAGGTTTGATTTCTTCGGCTTGCGAT
ATTTTCTATCTAAAAGATAAACTCGAATTAATCTATGAAAGATTATCTAATAAAAATCAACTACTTACCAAGCAAAATGC
TTCTCAGTCAATGCAGATTAAGTCAATTATGAAGCTATTAAATGAGGTCGAGAGAGCAAAAAATATTGATTTTTATAGAC
TAATTTTTGGTTTGGGCATACGAAATGTAGGCCTAAAAGCAGCAAAAATTCTTTCACGTTATGCAAGTAATCTTTCAGAA
TTGAGAAATTTAGACTTTAATTTACTTAAAAATCAGCATGATTTTGGCCCTGTTATTATTGAATCTCTAATCGATTATTT
TAATAATCAAAAAAATTCTGAGCAATTAAATTGTCTTGAAACTGTTGGGTTTAATTTTAAAACTACTTTTTTAACTAATC
AGTCAAATTCCTGAGCTAGTTTTGCAATTTCGGGAAAATTGAGCAAGCCACGCGATGAATATGTTAGAATTATTGAGGAA
TCAGGCGCTTCTTTTCATGAATCACTTACAAAAAAAACTGATTTTTTATTGCTAGGCCAATCTGCGGGTTCAAAGATAGA
AAAGGCTAAGAAAGCCGGGATAAAAATCATTAACGAGGTACAATTTTTTGATTTAATTAAAAATTCTAAAAAAACTTAA

Upstream 100 bases:

>100_bases
TATTAGTTTAATTATTTTAACAGTTTTAATGGAATTAAATATAATTCCAATTTCGCATCTGCCAAAATGATGATTATACA
CTAATCAAAAAATTTAAATT

Downstream 100 bases:

>100_bases
ATTAAAAAAATACTATAAATTACGTTGTTTTTTTATTTTTTCGAATCACAATAGAAGAAAGTTTAGACTATGGCAATTGC
AATAATCGCTGAATATAACC

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 692; Mature: 692

Protein sequence:

>692_residues
MKKNSQIRAKIIELREKIEKWNHHYYQLQNPLVDDLVYDKTLRELEKLERENFFLFSLEELNQSPSQKVGSKITSKFEKV
AHSSPMLSLNKAYSNEELEKWAKKAREILKTVTFFVEPKIDGIALSLFYQNGNLIKALTRGDGVFGENVLVNALKINDEF
IPKKINYLEDLEVRGEIYIDNSTFASLQLETKKFKNPRNAASGILRRYKNHKPKIDAKSIKFLEEESDFRYLKSFFYTLV
NPEKHKINSQFDSTNFLRNLGFQVNPFQKKCSDLKDVFNFISIIKQKRDFLNYNIDGVVVKVNEFSIYEKLGSTSKFPHS
AIAFKFEDDIAKTKLLAIFATIGRTGKVTYNAKIEPVTLAGSKISSAILPNYSYIENLKLNLNTEVYIKKAGEIIPQIIG
SVHNYPKTNFSIVKNCPKCNSELVNSESGLDQFCQNQFCPEIILQKIVHFCSKNALNIESLAQKRIEKFLEKGLISSACD
IFYLKDKLELIYERLSNKNQLLTKQNASQSMQIKSIMKLLNEVERAKNIDFYRLIFGLGIRNVGLKAAKILSRYASNLSE
LRNLDFNLLKNQHDFGPVIIESLIDYFNNQKNSEQLNCLETVGFNFKTTFLTNQSNSWASFAISGKLSKPRDEYVRIIEE
SGASFHESLTKKTDFLLLGQSAGSKIEKAKKAGIKIINEVQFFDLIKNSKKT

Sequences:

>Translated_692_residues
MKKNSQIRAKIIELREKIEK*NHHYYQLQNPLVDDLVYDKTLRELEKLERENFFLFSLEELNQSPSQKVGSKITSKFEKV
AHSSPMLSLNKAYSNEELEK*AKKAREILKTVTFFVEPKIDGIALSLFYQNGNLIKALTRGDGVFGENVLVNALKINDEF
IPKKINYLEDLEVRGEIYIDNSTFASLQLETKKFKNPRNAASGILRRYKNHKPKIDAKSIKFLEEESDFRYLKSFFYTLV
NPEKHKINSQFDSTNFLRNLGFQVNPFQKKCSDLKDVFNFISIIKQKRDFLNYNIDGVVVKVNEFSIYEKLGSTSKFPHS
AIAFKFEDDIAKTKLLAIFATIGRTGKVTYNAKIEPVTLAGSKISSAILPNYSYIENLKLNLNTEVYIKKAGEIIPQIIG
SVHNYPKTNFSIVKNCPKCNSELVNSESGLDQFCQNQFCPEIILQKIVHFCSKNALNIESLAQKRIEKFLEKGLISSACD
IFYLKDKLELIYERLSNKNQLLTKQNASQSMQIKSIMKLLNEVERAKNIDFYRLIFGLGIRNVGLKAAKILSRYASNLSE
LRNLDFNLLKNQHDFGPVIIESLIDYFNNQKNSEQLNCLETVGFNFKTTFLTNQSNS*ASFAISGKLSKPRDEYVRIIEE
SGASFHESLTKKTDFLLLGQSAGSKIEKAKKAGIKIINEVQFFDLIKNSKKT
>Mature_692_residues
MKKNSQIRAKIIELREKIEK*NHHYYQLQNPLVDDLVYDKTLRELEKLERENFFLFSLEELNQSPSQKVGSKITSKFEKV
AHSSPMLSLNKAYSNEELEK*AKKAREILKTVTFFVEPKIDGIALSLFYQNGNLIKALTRGDGVFGENVLVNALKINDEF
IPKKINYLEDLEVRGEIYIDNSTFASLQLETKKFKNPRNAASGILRRYKNHKPKIDAKSIKFLEEESDFRYLKSFFYTLV
NPEKHKINSQFDSTNFLRNLGFQVNPFQKKCSDLKDVFNFISIIKQKRDFLNYNIDGVVVKVNEFSIYEKLGSTSKFPHS
AIAFKFEDDIAKTKLLAIFATIGRTGKVTYNAKIEPVTLAGSKISSAILPNYSYIENLKLNLNTEVYIKKAGEIIPQIIG
SVHNYPKTNFSIVKNCPKCNSELVNSESGLDQFCQNQFCPEIILQKIVHFCSKNALNIESLAQKRIEKFLEKGLISSACD
IFYLKDKLELIYERLSNKNQLLTKQNASQSMQIKSIMKLLNEVERAKNIDFYRLIFGLGIRNVGLKAAKILSRYASNLSE
LRNLDFNLLKNQHDFGPVIIESLIDYFNNQKNSEQLNCLETVGFNFKTTFLTNQSNS*ASFAISGKLSKPRDEYVRIIEE
SGASFHESLTKKTDFLLLGQSAGSKIEKAKKAGIKIINEVQFFDLIKNSKKT

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=710, Percent_Identity=30.2816901408451, Blast_Score=316, Evalue=4e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 78858; Mature: 78858

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNSQIRAKIIELREKIEKNHHYYQLQNPLVDDLVYDKTLRELEKLERENFFLFSLEEL
CCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEEHHHH
NQSPSQKVGSKITSKFEKVAHSSPMLSLNKAYSNEELEKAKKAREILKTVTFFVEPKIDG
CCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IALSLFYQNGNLIKALTRGDGVFGENVLVNALKINDEFIPKKINYLEDLEVRGEIYIDNS
EEEEEEEECCCEEEEEECCCCCCCCCCEEEEEEECCHHCCHHHHHHHCCEECCEEEECCC
TFASLQLETKKFKNPRNAASGILRRYKNHKPKIDAKSIKFLEEESDFRYLKSFFYTLVNP
CEEEEEEEHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCC
EKHKINSQFDSTNFLRNLGFQVNPFQKKCSDLKDVFNFISIIKQKRDFLNYNIDGVVVKV
HHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
NEFSIYEKLGSTSKFPHSAIAFKFEDDIAKTKLLAIFATIGRTGKVTYNAKIEPVTLAGS
ECHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCEEEEECEECEEEECCC
KISSAILPNYSYIENLKLNLNTEVYIKKAGEIIPQIIGSVHNYPKTNFSIVKNCPKCNSE
HHHHHHCCCCHHHHCCEEECCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHH
LVNSESGLDQFCQNQFCPEIILQKIVHFCSKNALNIESLAQKRIEKFLEKGLISSACDIF
HHCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YLKDKLELIYERLSNKNQLLTKQNASQSMQIKSIMKLLNEVERAKNIDFYRLIFGLGIRN
HHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHH
VGLKAAKILSRYASNLSELRNLDFNLLKNQHDFGPVIIESLIDYFNNQKNSEQLNCLETV
CCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHC
GFNFKTTFLTNQSNSASFAISGKLSKPRDEYVRIIEESGASFHESLTKKTDFLLLGQSAG
CCCEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCC
SKIEKAKKAGIKIINEVQFFDLIKNSKKT
HHHHHHHHHCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKNSQIRAKIIELREKIEKNHHYYQLQNPLVDDLVYDKTLRELEKLERENFFLFSLEEL
CCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEEHHHH
NQSPSQKVGSKITSKFEKVAHSSPMLSLNKAYSNEELEKAKKAREILKTVTFFVEPKIDG
CCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IALSLFYQNGNLIKALTRGDGVFGENVLVNALKINDEFIPKKINYLEDLEVRGEIYIDNS
EEEEEEEECCCEEEEEECCCCCCCCCCEEEEEEECCHHCCHHHHHHHCCEECCEEEECCC
TFASLQLETKKFKNPRNAASGILRRYKNHKPKIDAKSIKFLEEESDFRYLKSFFYTLVNP
CEEEEEEEHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCC
EKHKINSQFDSTNFLRNLGFQVNPFQKKCSDLKDVFNFISIIKQKRDFLNYNIDGVVVKV
HHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
NEFSIYEKLGSTSKFPHSAIAFKFEDDIAKTKLLAIFATIGRTGKVTYNAKIEPVTLAGS
ECHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCEEEEECEECEEEECCC
KISSAILPNYSYIENLKLNLNTEVYIKKAGEIIPQIIGSVHNYPKTNFSIVKNCPKCNSE
HHHHHHCCCCHHHHCCEEECCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHH
LVNSESGLDQFCQNQFCPEIILQKIVHFCSKNALNIESLAQKRIEKFLEKGLISSACDIF
HHCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YLKDKLELIYERLSNKNQLLTKQNASQSMQIKSIMKLLNEVERAKNIDFYRLIFGLGIRN
HHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHH
VGLKAAKILSRYASNLSELRNLDFNLLKNQHDFGPVIIESLIDYFNNQKNSEQLNCLETV
CCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHC
GFNFKTTFLTNQSNSASFAISGKLSKPRDEYVRIIEESGASFHESLTKKTDFLLLGQSAG
CCCEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCC
SKIEKAKKAGIKIINEVQFFDLIKNSKKT
HHHHHHHHHCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA