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The map label for this gene is smpB

Identifier: 54020341

GI number: 54020341

Start: 130585

End: 131022

Strand: Reverse

Name: smpB

Synonym: mhp110

Alternate gene names: 54020341

Gene position: 131022-130585 (Counterclockwise)

Preceding gene: 54020342

Following gene: 54020340

Centisome position: 14.68

GC content: 30.37

Gene sequence:

>438_bases
ATGGAAATTTTAATAAAAAATAAGCGCGCCAATTTTGACTATGAAATTATTGATAGATTTACTGCCGGAATTGTTCTACT
TGGTTGAGAGGTTAAGTCAATTCAAGCAAAAAATATTTCGCTTGTTGGTGCTTTTTGTTATTTTAAAGGTCATGAACTTT
TTCTTTCGAATGCAAAAATTAGCGAATATAAAGGTTCGCGTGGTCAAACTGATCGAAGTCGTAAACTTTTAATGCACAAA
CACGAACTCAAAAAAATTCTAAAAGAAAAAATTACAAGAAAACTAGCAATTATCCCACTTTTTATTGGGCAGAAAAATCG
AAAAATTAAACTCGAGATCGCACTTGCAAAAGGAAAAACAAAACTGGATAAACGTAATTTGATTAAAGAACGTGATCAAA
AAAGGGAGGCCGCAAAATTTTTAAAAAATTATTACTAA

Upstream 100 bases:

>100_bases
TTTTTTGACATTTTAGTCCCTTTTTTTATAAAATTGTATAAATTAGATATAAAATAAATTACTATTAGGTTATAATTATA
ATATTATTTTAGGTTTAAAA

Downstream 100 bases:

>100_bases
TTTTTGGTTTATTACCGCTATTTTCAATTTCAAGTTGTTATGATCTTTTTACTAGCTTCTTAAAAGATGAAAATACTATC
AAAGATGGAAATAGTCCAAT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MEILIKNKRANFDYEIIDRFTAGIVLLGWEVKSIQAKNISLVGAFCYFKGHELFLSNAKISEYKGSRGQTDRSRKLLMHK
HELKKILKEKITRKLAIIPLFIGQKNRKIKLEIALAKGKTKLDKRNLIKERDQKREAAKFLKNYY

Sequences:

>Translated_145_residues
MEILIKNKRANFDYEIIDRFTAGIVLLG*EVKSIQAKNISLVGAFCYFKGHELFLSNAKISEYKGSRGQTDRSRKLLMHK
HELKKILKEKITRKLAIIPLFIGQKNRKIKLEIALAKGKTKLDKRNLIKERDQKREAAKFLKNYY
>Mature_145_residues
MEILIKNKRANFDYEIIDRFTAGIVLLG*EVKSIQAKNISLVGAFCYFKGHELFLSNAKISEYKGSRGQTDRSRKLLMHK
HELKKILKEKITRKLAIIPLFIGQKNRKIKLEIALAKGKTKLDKRNLIKERDQKREAAKFLKNYY

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=143, Percent_Identity=32.8671328671329, Blast_Score=70, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 16790; Mature: 16790

Theoretical pI: Translated: 10.87; Mature: 10.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEILIKNKRANFDYEIIDRFTAGIVLLGEVKSIQAKNISLVGAFCYFKGHELFLSNAKIS
CCEEEECCCCCCCHHHHHHHHHCEEEEECCCCCCCCCEEEEEEEEEECCCEEEEECCCHH
EYKGSRGQTDRSRKLLMHKHELKKILKEKITRKLAIIPLFIGQKNRKIKLEIALAKGKTK
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEEEEECCCCC
LDKRNLIKERDQKREAAKFLKNYY
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEILIKNKRANFDYEIIDRFTAGIVLLGEVKSIQAKNISLVGAFCYFKGHELFLSNAKIS
CCEEEECCCCCCCHHHHHHHHHCEEEEECCCCCCCCCEEEEEEEEEECCCEEEEECCCHH
EYKGSRGQTDRSRKLLMHKHELKKILKEKITRKLAIIPLFIGQKNRKIKLEIALAKGKTK
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEEEEECCCCC
LDKRNLIKERDQKREAAKFLKNYY
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA