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The map label for this gene is pheS

Identifier: 54020339

GI number: 54020339

Start: 122463

End: 123143

Strand: Reverse

Name: pheS

Synonym: mhp106

Alternate gene names: 54020339

Gene position: 123143-122463 (Counterclockwise)

Preceding gene: 54020340

Following gene: 54020338

Centisome position: 13.79

GC content: 29.81

Gene sequence:

>681_bases
ATGCCATATTCCCTTAATTTAATTTCAAGTTTGCTAAAAAGAATGACAGATTTTCTAACTATCTTTGATTTTAAATATAT
TAAATCTAACGAATTAACAACTTTTGAGTATAATTTTATCAAACTTAATATTGACTCAAAACATCCAGCTTTTGATCCTA
GTCAGAGTTTAGTTTTTAGCGATAATCAATTGCTTGCAACTCATAAAACCGGAATTTCAATCGAAATTATAAGTAAATAC
CCTAACCAAAAATTAGCCTTTTACTCCTATGGAAAAGTTTTCCGTAATGACGAAGAAGATGCAACGCATTCTCATCAATT
TAATCAACTAGATTTAGTAGCAACTGGAAATTATAGTGTTTCTCATCTTAAAAGTTTAATTAGCGATTTACTTGAATATG
TTTTTGAAGAAAAACTTAAAACTCGGTTTCGCCCTTCTTACTTTCCATTTACTCAACCTTCTTTTGAGGTTGATATTTTC
TATCATAATCGCTGAATTGAAATTTTAGGTTGTGGACTTTTACATCCAAAGGTGATGAAAAATGCAGGATTTAGTGCAAA
AAAAATCTATGGAATTGCTGCAGGAATAGGAATTGAACGGCTAGCGATGGTCAAATACGGAATTAGCGATATTCGGGAAT
TTTATAAAAATGATTTACGTTTTTTAAAACAATTTAGGTAG

Upstream 100 bases:

>100_bases
CCTATTATAGTATAATTGATAAACATTATAATTAAGTAATTGAAATAATAAAATTTTAATTCTTAAGAAAACCAATATTT
AGAATCGAGATTTAAAAAAT

Downstream 100 bases:

>100_bases
GTAGAGAGTAATGCTTTTTTCATTAAGAAGACTAAAAAAATTAGCAAATTTAGAGGCTTTTAGTGACCAAAAAGTAATTG
ATAGTTTAATAAATCTTGGT

Product: phenylalanyl-tRNA synthetase alpha chain

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MPYSLNLISSLLKRMTDFLTIFDFKYIKSNELTTFEYNFIKLNIDSKHPAFDPSQSLVFSDNQLLATHKTGISIEIISKY
PNQKLAFYSYGKVFRNDEEDATHSHQFNQLDLVATGNYSVSHLKSLISDLLEYVFEEKLKTRFRPSYFPFTQPSFEVDIF
YHNRWIEILGCGLLHPKVMKNAGFSAKKIYGIAAGIGIERLAMVKYGISDIREFYKNDLRFLKQFR

Sequences:

>Translated_226_residues
MPYSLNLISSLLKRMTDFLTIFDFKYIKSNELTTFEYNFIKLNIDSKHPAFDPSQSLVFSDNQLLATHKTGISIEIISKY
PNQKLAFYSYGKVFRNDEEDATHSHQFNQLDLVATGNYSVSHLKSLISDLLEYVFEEKLKTRFRPSYFPFTQPSFEVDIF
YHNR*IEILGCGLLHPKVMKNAGFSAKKIYGIAAGIGIERLAMVKYGISDIREFYKNDLRFLKQFR
>Mature_225_residues
PYSLNLISSLLKRMTDFLTIFDFKYIKSNELTTFEYNFIKLNIDSKHPAFDPSQSLVFSDNQLLATHKTGISIEIISKYP
NQKLAFYSYGKVFRNDEEDATHSHQFNQLDLVATGNYSVSHLKSLISDLLEYVFEEKLKTRFRPSYFPFTQPSFEVDIFY
HNR*IEILGCGLLHPKVMKNAGFSAKKIYGIAAGIGIERLAMVKYGISDIREFYKNDLRFLKQFR

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=230, Percent_Identity=30.4347826086957, Blast_Score=105, Evalue=2e-23,
Organism=Homo sapiens, GI4758340, Length=159, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1788007, Length=225, Percent_Identity=39.5555555555556, Blast_Score=173, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI32566635, Length=236, Percent_Identity=29.6610169491525, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32563657, Length=158, Percent_Identity=29.746835443038, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17508957, Length=158, Percent_Identity=29.746835443038, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6325304, Length=119, Percent_Identity=34.453781512605, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321087, Length=161, Percent_Identity=30.4347826086957, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI17137424, Length=249, Percent_Identity=29.718875502008, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI18858079, Length=180, Percent_Identity=32.2222222222222, Blast_Score=85, Evalue=4e-17,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR022911
- InterPro:   IPR002319 [H]

Pfam domain/function: PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 26160; Mature: 26029

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYSLNLISSLLKRMTDFLTIFDFKYIKSNELTTFEYNFIKLNIDSKHPAFDPSQSLVFS
CCCCHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEEEEEEEECCCCCCCCCCCCEEEC
DNQLLATHKTGISIEIISKYPNQKLAFYSYGKVFRNDEEDATHSHQFNQLDLVATGNYSV
CCEEEEEECCCCEEEEEECCCCCEEEEEEHHHHHCCCCCCCHHCCCCCEEEEEEECCCCH
SHLKSLISDLLEYVFEEKLKTRFRPSYFPFTQPSFEVDIFYHNRIEILGCGLLHPKVMKN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCEEEEEECCCCHHHHHC
AGFSAKKIYGIAAGIGIERLAMVKYGISDIREFYKNDLRFLKQFR
CCCCHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PYSLNLISSLLKRMTDFLTIFDFKYIKSNELTTFEYNFIKLNIDSKHPAFDPSQSLVFS
CCCHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEEEEEEEECCCCCCCCCCCCEEEC
DNQLLATHKTGISIEIISKYPNQKLAFYSYGKVFRNDEEDATHSHQFNQLDLVATGNYSV
CCEEEEEECCCCEEEEEECCCCCEEEEEEHHHHHCCCCCCCHHCCCCCEEEEEEECCCCH
SHLKSLISDLLEYVFEEKLKTRFRPSYFPFTQPSFEVDIFYHNRIEILGCGLLHPKVMKN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCEEEEEECCCCHHHHHC
AGFSAKKIYGIAAGIGIERLAMVKYGISDIREFYKNDLRFLKQFR
CCCCHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12949158 [H]