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The map label for this gene is parE

Identifier: 54020320

GI number: 54020320

Start: 44235

End: 46160

Strand: Reverse

Name: parE

Synonym: mhp035

Alternate gene names: 54020320

Gene position: 46160-44235 (Counterclockwise)

Preceding gene: 54020321

Following gene: 54020319

Centisome position: 5.17

GC content: 31.57

Gene sequence:

>1926_bases
ATGACCTACTCAGTAGAAAAATTAAAACTTTTAAAAGGGCTTGAAGCGGTCCGCAAACGCCCGGGAATGTATATTGGATC
AACTGATAAAAACGGATTACACCAGCTAATTTGGGAAATCTTTGATAATGCAATTGACGAGGCAATTGCTGGATTTGCAA
ATGAAATTTCACTAATAATTAATAGTGATAATTCTATTGAAATTAGTGATAATGGGCGCGGAATCCCGACAGAAATTCAT
AAAAAAACCGGAAAAACTGGCGTTGAATTAGTTTTTAGCGAGCTCCATTCAGGAGCGAAATTTTCTACTGATATCTACAA
AACCGCTGGTGGCCTTCATGGTGTTGGCTCTTCAGTTGTTAATGCTCTTTCTGAAAAACTAGAAGTCTATATAAATCGGG
ATAAAAAATTGTTCTATACTAGTTTTATTAACGGTGGAAAAATTGAAACTCGAACTAAAATTATAGGAAAAAGCGCTACA
ACTGGGACAAAAATCAGATTTTGACCTGATTTTTCCCTTTTTTCACATTCAGAATTTGACTCAGAAATGATAATTTCGCG
CCTACGCGAAACTTGTTTTTTGATTAACAATCTAAAAATCAATTTTTTTGATACAAAAAACAATATTGAAAAAAAGTTTC
TCTGCGAAAGGGGAATCGAAAGTTTTATTGAATTTTTGAACAATGATCAAGAAAAAATTCATGATAAAGTTATTACTTTT
AAAGAAAAAATGCAGGAAATCATAGTGGAATTTGGATTTCAGTATGTTAATTCACAGCAAGAAAATATTATCTCTTTTGT
CAATAATGTAAAAACAAATTTAGGGGGAAGTCACGAAAATGGTTTTAAATCCGGTTTTTTAAAGGCAATTAATACTTACG
GCCAACAAAATAATTTACTTAAAACTAAGCAAATTTTAGAATTTAGTGATGCAAAAGTTGGACTTTCAGCTGTTTTATCA
CTGCGAATTCCTGAATCAATTCTTGAATTTGTTGGGCAAACAAAAAATAAATTATCAACTATTTTAGCAAAAACAGTTAC
AGAAGAGGTAATTTTTACAAATTTAATGACTTTTTTTGCACTAAATAAAGAAGTTGCAAGTAAAATAATTAATTTTATAC
TAGAAACTTATACCCAAAGACAAAAGTCAAAATTAAGCATAATTGAGTCAAAAACAAGCAAATCAATTACAAAAGAAAAG
CGGATTTTATCAGGGAAATTAACCCCAGCAAATTCAAAAAAAGCTATGAATCGCGAGCTTTTTTTGGTCGAAGGTGAATC
GGCAGGTGGTTCTGCAAAACTTGCAAGAAACCGTGAGTTTCAAGCAATTTTACCTTTAAAAGGTAAAATTGTAAACGCCC
AAAAAACAAGGTTAATTGATCTATTAAAAAATGAGGAAATTATCGCAATTATTAGTGCATTAGGAACTGGAATTGGTCAG
AATTTTAACCTTAAGAACCTAAATTATGGCAAAATTATCATCATGACTGACGCTGACAATGACGGAGCTCACATCCAAAT
TTTAATTTTAACCTTTCTTTTTTTGCATATGCGTCCTTTAATCGAAAAAGGTTTTGTCTATATTGCTCAACCGCCCTTAT
ATCGAATTAGTGAAAAGGGGAGAAAAGATATTTATATCTGGGATGAGGAAAAGTTCGAAAAATACATCAAAAACAGTACA
AATTTGCAAATTCAGCGCTATAAAGGACTTGGGGAAATGAATGCAAATCAGCTTTGGCAAACGACAATGGACCCCGAAAG
GCGAACTTTGGAAAAAGTTTTAATCGAAAATTATCCAAAAGTAGAGGAAAATTTTCAAATTTTAATGGGCGAAAGAGCTG
ATCTAAGAAAGAGCTGAATTGAAAAAAACGTTGATTTTTCCCTTGAAGACAAATTTATTGAAAATTTAAAGGAAACATAT
GAATAA

Upstream 100 bases:

>100_bases
TAAATTTTACATTAAAATTTTTGTAAAATTATTTACCTTTTTGTATAAAATTTAATTTAGAAAAATTTTTACGCATTTTC
AGAAAGGATTTAGACCCTAT

Downstream 100 bases:

>100_bases
AAGTCTTGATTCAGTAATTAATTCCCGGTTAGAAAAAATTTTAGCAGAAAAATTTATCCGTTATTCAAAATATGTAATTC
AACAACGGGCAATTCCAGAT

Product: topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 641; Mature: 640

Protein sequence:

>641_residues
MTYSVEKLKLLKGLEAVRKRPGMYIGSTDKNGLHQLIWEIFDNAIDEAIAGFANEISLIINSDNSIEISDNGRGIPTEIH
KKTGKTGVELVFSELHSGAKFSTDIYKTAGGLHGVGSSVVNALSEKLEVYINRDKKLFYTSFINGGKIETRTKIIGKSAT
TGTKIRFWPDFSLFSHSEFDSEMIISRLRETCFLINNLKINFFDTKNNIEKKFLCERGIESFIEFLNNDQEKIHDKVITF
KEKMQEIIVEFGFQYVNSQQENIISFVNNVKTNLGGSHENGFKSGFLKAINTYGQQNNLLKTKQILEFSDAKVGLSAVLS
LRIPESILEFVGQTKNKLSTILAKTVTEEVIFTNLMTFFALNKEVASKIINFILETYTQRQKSKLSIIESKTSKSITKEK
RILSGKLTPANSKKAMNRELFLVEGESAGGSAKLARNREFQAILPLKGKIVNAQKTRLIDLLKNEEIIAIISALGTGIGQ
NFNLKNLNYGKIIIMTDADNDGAHIQILILTFLFLHMRPLIEKGFVYIAQPPLYRISEKGRKDIYIWDEEKFEKYIKNST
NLQIQRYKGLGEMNANQLWQTTMDPERRTLEKVLIENYPKVEENFQILMGERADLRKSWIEKNVDFSLEDKFIENLKETY
E

Sequences:

>Translated_641_residues
MTYSVEKLKLLKGLEAVRKRPGMYIGSTDKNGLHQLIWEIFDNAIDEAIAGFANEISLIINSDNSIEISDNGRGIPTEIH
KKTGKTGVELVFSELHSGAKFSTDIYKTAGGLHGVGSSVVNALSEKLEVYINRDKKLFYTSFINGGKIETRTKIIGKSAT
TGTKIRF*PDFSLFSHSEFDSEMIISRLRETCFLINNLKINFFDTKNNIEKKFLCERGIESFIEFLNNDQEKIHDKVITF
KEKMQEIIVEFGFQYVNSQQENIISFVNNVKTNLGGSHENGFKSGFLKAINTYGQQNNLLKTKQILEFSDAKVGLSAVLS
LRIPESILEFVGQTKNKLSTILAKTVTEEVIFTNLMTFFALNKEVASKIINFILETYTQRQKSKLSIIESKTSKSITKEK
RILSGKLTPANSKKAMNRELFLVEGESAGGSAKLARNREFQAILPLKGKIVNAQKTRLIDLLKNEEIIAIISALGTGIGQ
NFNLKNLNYGKIIIMTDADNDGAHIQILILTFLFLHMRPLIEKGFVYIAQPPLYRISEKGRKDIYIWDEEKFEKYIKNST
NLQIQRYKGLGEMNANQLWQTTMDPERRTLEKVLIENYPKVEENFQILMGERADLRKS*IEKNVDFSLEDKFIENLKETY
E
>Mature_640_residues
TYSVEKLKLLKGLEAVRKRPGMYIGSTDKNGLHQLIWEIFDNAIDEAIAGFANEISLIINSDNSIEISDNGRGIPTEIHK
KTGKTGVELVFSELHSGAKFSTDIYKTAGGLHGVGSSVVNALSEKLEVYINRDKKLFYTSFINGGKIETRTKIIGKSATT
GTKIRF*PDFSLFSHSEFDSEMIISRLRETCFLINNLKINFFDTKNNIEKKFLCERGIESFIEFLNNDQEKIHDKVITFK
EKMQEIIVEFGFQYVNSQQENIISFVNNVKTNLGGSHENGFKSGFLKAINTYGQQNNLLKTKQILEFSDAKVGLSAVLSL
RIPESILEFVGQTKNKLSTILAKTVTEEVIFTNLMTFFALNKEVASKIINFILETYTQRQKSKLSIIESKTSKSITKEKR
ILSGKLTPANSKKAMNRELFLVEGESAGGSAKLARNREFQAILPLKGKIVNAQKTRLIDLLKNEEIIAIISALGTGIGQN
FNLKNLNYGKIIIMTDADNDGAHIQILILTFLFLHMRPLIEKGFVYIAQPPLYRISEKGRKDIYIWDEEKFEKYIKNSTN
LQIQRYKGLGEMNANQLWQTTMDPERRTLEKVLIENYPKVEENFQILMGERADLRKS*IEKNVDFSLEDKFIENLKETYE

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=644, Percent_Identity=25.9316770186335, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI19913406, Length=633, Percent_Identity=25.1184834123223, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI48994957, Length=558, Percent_Identity=40.8602150537634, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1789408, Length=603, Percent_Identity=34.9917081260365, Blast_Score=372, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17535065, Length=617, Percent_Identity=24.3111831442464, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI212645845, Length=552, Percent_Identity=25.5434782608696, Blast_Score=100, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324241, Length=628, Percent_Identity=25.9554140127389, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI17136538, Length=639, Percent_Identity=22.848200312989, Blast_Score=107, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005740 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72613; Mature: 72481

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYSVEKLKLLKGLEAVRKRPGMYIGSTDKNGLHQLIWEIFDNAIDEAIAGFANEISLII
CCCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
NSDNSIEISDNGRGIPTEIHKKTGKTGVELVFSELHSGAKFSTDIYKTAGGLHGVGSSVV
ECCCEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHH
NALSEKLEVYINRDKKLFYTSFINGGKIETRTKIIGKSATTGTKIRFPDFSLFSHSEFDS
HHHHHHHHHEECCCCEEEEEECCCCCCEEHHHHHHCCCCCCCCEEECCCCCCCCCCCCCH
EMIISRLRETCFLINNLKINFFDTKNNIEKKFLCERGIESFIEFLNNDQEKIHDKVITFK
HHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EKMQEIIVEFGFQYVNSQQENIISFVNNVKTNLGGSHENGFKSGFLKAINTYGQQNNLLK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHH
TKQILEFSDAKVGLSAVLSLRIPESILEFVGQTKNKLSTILAKTVTEEVIFTNLMTFFAL
HHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKEVASKIINFILETYTQRQKSKLSIIESKTSKSITKEKRILSGKLTPANSKKAMNRELF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEE
LVEGESAGGSAKLARNREFQAILPLKGKIVNAQKTRLIDLLKNEEIIAIISALGTGIGQN
EEECCCCCCCCHHHCCCCCEEECCCCCEEECHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
FNLKNLNYGKIIIMTDADNDGAHIQILILTFLFLHMRPLIEKGFVYIAQPPLYRISEKGR
CCCCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHCCCC
KDIYIWDEEKFEKYIKNSTNLQIQRYKGLGEMNANQLWQTTMDPERRTLEKVLIENYPKV
CCEEEECHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCC
EENFQILMGERADLRKSIEKNVDFSLEDKFIENLKETYE
CCCHHEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TYSVEKLKLLKGLEAVRKRPGMYIGSTDKNGLHQLIWEIFDNAIDEAIAGFANEISLII
CCCHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
NSDNSIEISDNGRGIPTEIHKKTGKTGVELVFSELHSGAKFSTDIYKTAGGLHGVGSSVV
ECCCEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHH
NALSEKLEVYINRDKKLFYTSFINGGKIETRTKIIGKSATTGTKIRFPDFSLFSHSEFDS
HHHHHHHHHEECCCCEEEEEECCCCCCEEHHHHHHCCCCCCCCEEECCCCCCCCCCCCCH
EMIISRLRETCFLINNLKINFFDTKNNIEKKFLCERGIESFIEFLNNDQEKIHDKVITFK
HHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EKMQEIIVEFGFQYVNSQQENIISFVNNVKTNLGGSHENGFKSGFLKAINTYGQQNNLLK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHH
TKQILEFSDAKVGLSAVLSLRIPESILEFVGQTKNKLSTILAKTVTEEVIFTNLMTFFAL
HHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKEVASKIINFILETYTQRQKSKLSIIESKTSKSITKEKRILSGKLTPANSKKAMNRELF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEE
LVEGESAGGSAKLARNREFQAILPLKGKIVNAQKTRLIDLLKNEEIIAIISALGTGIGQN
EEECCCCCCCCHHHCCCCCEEECCCCCEEECHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
FNLKNLNYGKIIIMTDADNDGAHIQILILTFLFLHMRPLIEKGFVYIAQPPLYRISEKGR
CCCCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHCCCC
KDIYIWDEEKFEKYIKNSTNLQIQRYKGLGEMNANQLWQTTMDPERRTLEKVLIENYPKV
CCEEEECHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCC
EENFQILMGERADLRKSIEKNVDFSLEDKFIENLKETYE
CCCHHEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7828871 [H]