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The map label for this gene is scrA [H]

Identifier: 54020275

GI number: 54020275

Start: 785500

End: 786096

Strand: Direct

Name: scrA [H]

Synonym: mhp629

Alternate gene names: 54020275

Gene position: 785500-786096 (Clockwise)

Preceding gene: 54020274

Following gene: 54020276

Centisome position: 87.99

GC content: 31.66

Gene sequence:

>597_bases
ATGGGTTTTTGTCTTAAAGCTTTATTTAGTAGGACAACAAAAAAATGTTGTAAAAATTGTAAATGCAAACAAAATAACGA
CTGTAAATGCAAATATGAAGCTTGTGAATGCAATACATCTGAGCAAATTTTTGCTCCGGCTACAGGAAAATTGATTAAAC
TTTCTAGGGTAAAAGATGGTATATTTTCTAAGGAAATGCTAGGGAAAGGATTCGCAGTCCAGGTAGGAAATACCGGAAAA
AAGGACATTTTTGCTCCAATTTCTGGAAAAGTTAATATGGTTTTTCCCACAAAACATGCCCTTGGCTTTGCATCTTTAGA
TAATAAGACACAAATTCTTCTACATCTTGGAATCGATACAGTTGAACTTGAAGGAAAAGGAATTGAAATTTTTGTTAAAC
TTAATCAAAAAATTCAAGCTGGCGATAAGATTGCCGCTGTTAATCTTGATTATCTAATTAAATCAGGAATTACTAACACT
GATATTATTGTTATTGTCCTTAATGAATCAGAATACAACAACTTTAAATTTCTAACTTCTCCACAGCGAGAAATCGACAC
TATTCCATTTTTAGTTGGAACAAGTAATAAATCCTAA

Upstream 100 bases:

>100_bases
AAAAAATATAATTTTTAAACTAAACAACAAAAAAATTAAAAAATAATGTAAAATTTAAGGAAATAAGTTTATTACTTATA
CTTAGATTTAGGAGGAAAAA

Downstream 100 bases:

>100_bases
GAACAATTTGATTTATGCCTTTTATTTCTTAAGTATGGCTAATTATACACTAATTTTTTTATTCCAAACTTTTTTTTTTT
TTTTTTACGGAAAAGAACAA

Product: hypothetical protein

Products: NA

Alternate protein names: EIIBCA-Scr; EII-Scr; Sucrose-specific phosphotransferase enzyme IIB component; PTS system sucrose-specific EIIB component; Sucrose permease IIC component; PTS system sucrose-specific EIIC component; Sucrose-specific phosphotransferase enzyme IIA component; PTS system sucrose-specific EIIA component [H]

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MGFCLKALFSRTTKKCCKNCKCKQNNDCKCKYEACECNTSEQIFAPATGKLIKLSRVKDGIFSKEMLGKGFAVQVGNTGK
KDIFAPISGKVNMVFPTKHALGFASLDNKTQILLHLGIDTVELEGKGIEIFVKLNQKIQAGDKIAAVNLDYLIKSGITNT
DIIVIVLNESEYNNFKFLTSPQREIDTIPFLVGTSNKS

Sequences:

>Translated_198_residues
MGFCLKALFSRTTKKCCKNCKCKQNNDCKCKYEACECNTSEQIFAPATGKLIKLSRVKDGIFSKEMLGKGFAVQVGNTGK
KDIFAPISGKVNMVFPTKHALGFASLDNKTQILLHLGIDTVELEGKGIEIFVKLNQKIQAGDKIAAVNLDYLIKSGITNT
DIIVIVLNESEYNNFKFLTSPQREIDTIPFLVGTSNKS
>Mature_197_residues
GFCLKALFSRTTKKCCKNCKCKQNNDCKCKYEACECNTSEQIFAPATGKLIKLSRVKDGIFSKEMLGKGFAVQVGNTGKK
DIFAPISGKVNMVFPTKHALGFASLDNKTQILLHLGIDTVELEGKGIEIFVKLNQKIQAGDKIAAVNLDYLIKSGITNTD
IIVIVLNESEYNNFKFLTSPQREIDTIPFLVGTSNKS

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790159, Length=125, Percent_Identity=33.6, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788757, Length=111, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786894, Length=111, Percent_Identity=34.2342342342342, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010973 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 21811; Mature: 21680

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS51093 PTS_EIIA_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
4.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFCLKALFSRTTKKCCKNCKCKQNNDCKCKYEACECNTSEQIFAPATGKLIKLSRVKDG
CCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEEEECCCCCEEEEEEHHCC
IFSKEMLGKGFAVQVGNTGKKDIFAPISGKVNMVFPTKHALGFASLDNKTQILLHLGIDT
CCCHHHHCCCEEEEECCCCCCEEEEECCCCEEEEECCHHHEEEEECCCCEEEEEEECCEE
VELEGKGIEIFVKLNQKIQAGDKIAAVNLDYLIKSGITNTDIIVIVLNESEYNNFKFLTS
EEECCCCEEEEEEECCEECCCCEEEEEEHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEC
PQREIDTIPFLVGTSNKS
CCHHCCCCEEEEECCCCC
>Mature Secondary Structure 
GFCLKALFSRTTKKCCKNCKCKQNNDCKCKYEACECNTSEQIFAPATGKLIKLSRVKDG
CHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEEEECCCCCEEEEEEHHCC
IFSKEMLGKGFAVQVGNTGKKDIFAPISGKVNMVFPTKHALGFASLDNKTQILLHLGIDT
CCCHHHHCCCEEEEECCCCCCEEEEECCCCEEEEECCHHHEEEEECCCCEEEEEEECCEE
VELEGKGIEIFVKLNQKIQAGDKIAAVNLDYLIKSGITNTDIIVIVLNESEYNNFKFLTS
EEECCCCEEEEEEECCEECCCCEEEEEEHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEC
PQREIDTIPFLVGTSNKS
CCHHCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2536656; 12397186; 8336109 [H]