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The map label for this gene is metG

Identifier: 54020190

GI number: 54020190

Start: 487231

End: 488793

Strand: Direct

Name: metG

Synonym: mhp410

Alternate gene names: 54020190

Gene position: 487231-488793 (Clockwise)

Preceding gene: 54020189

Following gene: 54020191

Centisome position: 54.58

GC content: 27.13

Gene sequence:

>1563_bases
ATGTTGCAAGAAAGGTCAAAGATGACTAAAAAATGTTATATCACAACTCCAATTTATTATGCTAGCGGGAGACTCCATAT
TGGGCATCTTTATACAACAGTGCTTGCTTGAGTAATTAGAAATTTTAAAAAAATGTCCGGATTTGAGGCTAGATTGTTTA
CTGGTTCTGATGAACATGGTCAAAAAATTTCTCAACTAGCTGCAAAAGCAAATCTTGAACCCCAAGAATTTGTTGATAAA
AATGTCGAAAATTTCAAATTTTTATGAGAAAAATTTGAAATCGACTATGATTATTTTCAAAGAACAACAAATTTAAATCA
TAAAAATTTTATCAAAAAAATTTTTCTAAAAATGTATGAAGATAATCATATTTTCAATGGCGAGTACCAAGGGCTTTATT
CGATAAGCGATGAGGAATTTCTCAGTCAAAGTCAAGCTGTTTATCAAAATAGAAATTATTTTCACCCTGTTAGCGGGGCA
AAAATGGAATTAATAAGTGAGAAATCCTACTTTTTTCCGATCAAAAAATTCCAAAAATGACTAGAGGATTTTCTTTTGGA
AAATCCCAAATTCATTTTTGATTCAAAAATCGCAAATGAATTAAGGCAGAATTTTTTACAAAAGGGACTTGAAGACTTAT
CAGTGACAAGAAAAAATCTAAAATGAGGTATTTTTCTTGATCAAAAATTTGAAAATCAGACAATTTATGTTTGACTTGAT
GCACTTTTTAGTTATTTATCAGTTTTTAAAGACCAAATTGATTTAGAAAATCCTAAGAAATCAAATTTTTGAACTGATTC
TGCCCAAATTATTCAAGTAGTTGGCAAAGAAATTGCCCGTTTTCACTGTATTTATTGGCCAATTTTTCTAAAATCGCTCA
ATTTTCGCCTTCCAAGCATAATTTTAACTCACGGTTGACTAATCACCCCTGAGGGAAAAATGTCAAAATCAAAAGGAAAT
GTGATAAATCCACTTGATTTGCTAAAAAAATTTGATCCAGAAATTATTAAATTTTATTTTGTCAGCCAAATAAACACTAA
AAATGATAGTGTTTTTGATGAAAATTTATTAGAAAATTTTTATAATTCTTTTTTTGTAAATACTTTTGGTAATTTAATAA
GTAGAACAATTGCCTTGATAGTGAAAAAATTTGATCAGCCTTTATTATTTGAAATTGCGGATCTTGATTGAACAGATATT
AGTTTTTATGAGAAAATCCTGCTTAGTTTAGATGAATTTTGCGATAACTTTGATCAACTAGAAATTGAAAAAGCATTTAA
AACAGTAATTGAATTAACAAAAAATTTAAACGGCTTTTTTGATATTAAACAACTTTGAAATCAAAAAAATTTAAAAAAAC
TAGCCCAAGGTTTAATTTTGGTTCTCAATGGAATTTATGCTATAACTGTTTTTTTAAGCATCGCAATGCCTAAAAAAACT
AACGAAATTCTTGAATTTCTTGGAATTAACGAGAAAAACTTTCTACTTATTACTAAATTAGATAAATTTGATAAAATTAA
TTTCAAAAAATTAGAAAAACCATTTTTTCCACGAAAAAATTAA

Upstream 100 bases:

>100_bases
GAATTTAAAGGGCAATGAGGGACAAACTATCTTCATAATCTTTATCTTCACCCGCAAAATAAAAATTTACATATCTATCG
TAGAGAAATTCAAAGATTAT

Downstream 100 bases:

>100_bases
TAACCTAAAATTCAACGATAATTATGTTTTTTGTTTAAATATTGCACCAGAGGAACCAAACGGATTAAAATTAGGGCATA
AATTGGGAAGGAAACAGTTG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MLQERSKMTKKCYITTPIYYASGRLHIGHLYTTVLAWVIRNFKKMSGFEARLFTGSDEHGQKISQLAAKANLEPQEFVDK
NVENFKFLWEKFEIDYDYFQRTTNLNHKNFIKKIFLKMYEDNHIFNGEYQGLYSISDEEFLSQSQAVYQNRNYFHPVSGA
KMELISEKSYFFPIKKFQKWLEDFLLENPKFIFDSKIANELRQNFLQKGLEDLSVTRKNLKWGIFLDQKFENQTIYVWLD
ALFSYLSVFKDQIDLENPKKSNFWTDSAQIIQVVGKEIARFHCIYWPIFLKSLNFRLPSIILTHGWLITPEGKMSKSKGN
VINPLDLLKKFDPEIIKFYFVSQINTKNDSVFDENLLENFYNSFFVNTFGNLISRTIALIVKKFDQPLLFEIADLDWTDI
SFYEKILLSLDEFCDNFDQLEIEKAFKTVIELTKNLNGFFDIKQLWNQKNLKKLAQGLILVLNGIYAITVFLSIAMPKKT
NEILEFLGINEKNFLLITKLDKFDKINFKKLEKPFFPRKN

Sequences:

>Translated_520_residues
MLQERSKMTKKCYITTPIYYASGRLHIGHLYTTVLA*VIRNFKKMSGFEARLFTGSDEHGQKISQLAAKANLEPQEFVDK
NVENFKFL*EKFEIDYDYFQRTTNLNHKNFIKKIFLKMYEDNHIFNGEYQGLYSISDEEFLSQSQAVYQNRNYFHPVSGA
KMELISEKSYFFPIKKFQK*LEDFLLENPKFIFDSKIANELRQNFLQKGLEDLSVTRKNLK*GIFLDQKFENQTIYV*LD
ALFSYLSVFKDQIDLENPKKSNF*TDSAQIIQVVGKEIARFHCIYWPIFLKSLNFRLPSIILTHG*LITPEGKMSKSKGN
VINPLDLLKKFDPEIIKFYFVSQINTKNDSVFDENLLENFYNSFFVNTFGNLISRTIALIVKKFDQPLLFEIADLD*TDI
SFYEKILLSLDEFCDNFDQLEIEKAFKTVIELTKNLNGFFDIKQL*NQKNLKKLAQGLILVLNGIYAITVFLSIAMPKKT
NEILEFLGINEKNFLLITKLDKFDKINFKKLEKPFFPRKN
>Mature_520_residues
MLQERSKMTKKCYITTPIYYASGRLHIGHLYTTVLA*VIRNFKKMSGFEARLFTGSDEHGQKISQLAAKANLEPQEFVDK
NVENFKFL*EKFEIDYDYFQRTTNLNHKNFIKKIFLKMYEDNHIFNGEYQGLYSISDEEFLSQSQAVYQNRNYFHPVSGA
KMELISEKSYFFPIKKFQK*LEDFLLENPKFIFDSKIANELRQNFLQKGLEDLSVTRKNLK*GIFLDQKFENQTIYV*LD
ALFSYLSVFKDQIDLENPKKSNF*TDSAQIIQVVGKEIARFHCIYWPIFLKSLNFRLPSIILTHG*LITPEGKMSKSKGN
VINPLDLLKKFDPEIIKFYFVSQINTKNDSVFDENLLENFYNSFFVNTFGNLISRTIALIVKKFDQPLLFEIADLD*TDI
SFYEKILLSLDEFCDNFDQLEIEKAFKTVIELTKNLNGFFDIKQL*NQKNLKKLAQGLILVLNGIYAITVFLSIAMPKKT
NEILEFLGINEKNFLLITKLDKFDKINFKKLEKPFFPRKN

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=510, Percent_Identity=29.4117647058824, Blast_Score=189, Evalue=5e-48,
Organism=Escherichia coli, GI1788432, Length=551, Percent_Identity=24.5009074410163, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI212645096, Length=499, Percent_Identity=29.8597194388778, Blast_Score=191, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI71992781, Length=396, Percent_Identity=27.7777777777778, Blast_Score=125, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6321610, Length=396, Percent_Identity=32.8282828282828, Blast_Score=184, Evalue=3e-47,
Organism=Drosophila melanogaster, GI21358483, Length=492, Percent_Identity=27.4390243902439, Blast_Score=178, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24655311, Length=465, Percent_Identity=22.7956989247312, Blast_Score=100, Evalue=5e-21,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 59741; Mature: 59741

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQERSKMTKKCYITTPIYYASGRLHIGHLYTTVLAVIRNFKKMSGFEARLFTGSDEHGQ
CCCCHHHHCCEEEEECCEEEECCCEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHH
KISQLAAKANLEPQEFVDKNVENFKFLEKFEIDYDYFQRTTNLNHKNFIKKIFLKMYEDN
HHHHHHHHCCCCHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHCCC
HIFNGEYQGLYSISDEEFLSQSQAVYQNRNYFHPVSGAKMELISEKSYFFPIKKFQKLED
CEECCCCCCEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHH
FLLENPKFIFDSKIANELRQNFLQKGLEDLSVTRKNLKGIFLDQKFENQTIYVLDALFSY
HHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEHHHHHHHHH
LSVFKDQIDLENPKKSNFTDSAQIIQVVGKEIARFHCIYWPIFLKSLNFRLPSIILTHGL
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
ITPEGKMSKSKGNVINPLDLLKKFDPEIIKFYFVSQINTKNDSVFDENLLENFYNSFFVN
CCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
TFGNLISRTIALIVKKFDQPLLFEIADLDTDISFYEKILLSLDEFCDNFDQLEIEKAFKT
HHHHHHHHHHHHHHHHCCCCCEEEHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VIELTKNLNGFFDIKQLNQKNLKKLAQGLILVLNGIYAITVFLSIAMPKKTNEILEFLGI
HHHHHHCCCCEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
NEKNFLLITKLDKFDKINFKKLEKPFFPRKN
CCCCEEEEEECCCCCCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MLQERSKMTKKCYITTPIYYASGRLHIGHLYTTVLAVIRNFKKMSGFEARLFTGSDEHGQ
CCCCHHHHCCEEEEECCEEEECCCEEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHH
KISQLAAKANLEPQEFVDKNVENFKFLEKFEIDYDYFQRTTNLNHKNFIKKIFLKMYEDN
HHHHHHHHCCCCHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHCCC
HIFNGEYQGLYSISDEEFLSQSQAVYQNRNYFHPVSGAKMELISEKSYFFPIKKFQKLED
CEECCCCCCEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHH
FLLENPKFIFDSKIANELRQNFLQKGLEDLSVTRKNLKGIFLDQKFENQTIYVLDALFSY
HHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEHHHHHHHHH
LSVFKDQIDLENPKKSNFTDSAQIIQVVGKEIARFHCIYWPIFLKSLNFRLPSIILTHGL
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
ITPEGKMSKSKGNVINPLDLLKKFDPEIIKFYFVSQINTKNDSVFDENLLENFYNSFFVN
CCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
TFGNLISRTIALIVKKFDQPLLFEIADLDTDISFYEKILLSLDEFCDNFDQLEIEKAFKT
HHHHHHHHHHHHHHHHCCCCCEEEHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VIELTKNLNGFFDIKQLNQKNLKKLAQGLILVLNGIYAITVFLSIAMPKKTNEILEFLGI
HHHHHHCCCCEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
NEKNFLLITKLDKFDKINFKKLEKPFFPRKN
CCCCEEEEEECCCCCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11353084; 8636032 [H]