Click here to switch to the map view.

The map label for this gene is amiB

Identifier: 52842904

GI number: 52842904

Start: 3051166

End: 3052596

Strand: Reverse

Name: amiB

Synonym: lpg2698

Alternate gene names: 52842904

Gene position: 3052596-3051166 (Counterclockwise)

Preceding gene: 52842905

Following gene: 52842903

Centisome position: 89.84

GC content: 41.09

Gene sequence:

>1431_bases
ATGATGATGAGAGCGTGGGTCTTAAGTCTGTTATTATATCTACCAAGTTCTTTTGCTGTATTTTCTGCACAATTAAAGAA
TATTACAGTTTCTCAGCAACCAACCATGACGTCTCTTTATCTTTCTATACAAGGTTCATTTACCCATAAGCTATTTACCT
TAAGTCAGCCCAATCGTGTGGTTTTAGACTTAAGTGATACACAATTAGCCGTCAATATCAGTCAATTGGGTTTAATCAAT
TCGCTTATTCAGCAGGTAAGAAGCGGTAGTCCCTCCAAAAACACGCTCAGGCTTGTCATGGATGTAAAACAACCAGTCAA
GGTGCGCTCATCTTCATGGAAACCGAAAGGAGAATTGAATGGGATCAGAATTGATCTTGTTAATGATACTCCTTTAGTCA
TAAATAATCGCAGGCAGTTGAGTTCGCCTGTTAAAACGATTAAAGCTCCAGTCCAGCAAACCCAATCAAAAATATCCAGC
CAGCCATCAGAACAACGAATAACTGTGCATAAAACACCTGTTCAGGTTCCTCATAACCCATCAAAAAAACCTTTGCGTGA
TGTTGTTGTAGTGTTGGATGCAGGGCATGGGGGCAAAGATCCTGGTGCAAGAGGTCCGCGTCGTAGTAACGAGAAGGATG
TTGTGCTGGCCATTACTTTAAAACTTAAACGATTGATAGACAGACAACCAGGTATGCGCGCAGTCCTGACACGCTCGGGA
GATTATTACGTTGGACTTAGACAACGCCTGGATATTGCCAGAAAATATAATGGTGATGTATTCATTTCTATCCATGCCGA
TGCATTCAATAATCCTCACTCTAATGGAGCCTCGGTATTTGCCTTGTCACAAAGGGGGGCCACCAGTGAAGCAGCACGCT
GGTTAGCAGAAAAGGAGAATTACTCTGAACTAGGTGGAGTGAATTTAGGTGAATTGGATGATAGTAATGGGGTAGTACGT
TCTGTTTTGATCGATTTATCACAAACTGCAACAATAAATGCTGGTTTGGAAATGGGGGGAAAAGTATTAGGTCAATTGGA
TAATTTTACCAATTTGCATAATAACAAAGTGGAGCAAGCCCGTTTTGTTGTTCTGAAATCTCCAGATATTCCTTCTATTT
TGGTTGAAACCGGTTTTATCTCAAATCCCCGTGAGGAAAGAAATTTAACCAACCCTGCCTATCAGGAGCGATTGAGTCAG
GCAATATTTCAAGGACTAAAAAATTATTTTTGGGATCATCCTCCTCATGGGACTCGAATTGAGGCTATGATTTCTCCAAA
TTTTCATATCGTTAAAAAGGGTGAAACTCTTCCTGCTATCGCTGCTCGATATAGGGTATCTGTGAACGCATTACAATCAA
TTAACCGCCTTCAGGCAAGGGCAAGACTTATGCCCGGGCAAAAATTAGTGATACCTGGCACATGGTCTTAA

Upstream 100 bases:

>100_bases
ATATTCAGTTTAATTTAATAATTAAAGGGACTGGGCGCTTAATACAAATAGCTGCATTAAGTGTCGCGGGTAAAAGAATT
TTAGCTTGCCTGGCGGGTGA

Downstream 100 bases:

>100_bases
GGATACATCAACTTTCGCCAGCAATAGCCAATCAGATTGCAGCAGGAGAAGTTATAGAAAGACCTGCTTCGGTAGTCAAG
GAGCTGCTGGAAAACTCTTT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MMMRAWVLSLLLYLPSSFAVFSAQLKNITVSQQPTMTSLYLSIQGSFTHKLFTLSQPNRVVLDLSDTQLAVNISQLGLIN
SLIQQVRSGSPSKNTLRLVMDVKQPVKVRSSSWKPKGELNGIRIDLVNDTPLVINNRRQLSSPVKTIKAPVQQTQSKISS
QPSEQRITVHKTPVQVPHNPSKKPLRDVVVVLDAGHGGKDPGARGPRRSNEKDVVLAITLKLKRLIDRQPGMRAVLTRSG
DYYVGLRQRLDIARKYNGDVFISIHADAFNNPHSNGASVFALSQRGATSEAARWLAEKENYSELGGVNLGELDDSNGVVR
SVLIDLSQTATINAGLEMGGKVLGQLDNFTNLHNNKVEQARFVVLKSPDIPSILVETGFISNPREERNLTNPAYQERLSQ
AIFQGLKNYFWDHPPHGTRIEAMISPNFHIVKKGETLPAIAARYRVSVNALQSINRLQARARLMPGQKLVIPGTWS

Sequences:

>Translated_476_residues
MMMRAWVLSLLLYLPSSFAVFSAQLKNITVSQQPTMTSLYLSIQGSFTHKLFTLSQPNRVVLDLSDTQLAVNISQLGLIN
SLIQQVRSGSPSKNTLRLVMDVKQPVKVRSSSWKPKGELNGIRIDLVNDTPLVINNRRQLSSPVKTIKAPVQQTQSKISS
QPSEQRITVHKTPVQVPHNPSKKPLRDVVVVLDAGHGGKDPGARGPRRSNEKDVVLAITLKLKRLIDRQPGMRAVLTRSG
DYYVGLRQRLDIARKYNGDVFISIHADAFNNPHSNGASVFALSQRGATSEAARWLAEKENYSELGGVNLGELDDSNGVVR
SVLIDLSQTATINAGLEMGGKVLGQLDNFTNLHNNKVEQARFVVLKSPDIPSILVETGFISNPREERNLTNPAYQERLSQ
AIFQGLKNYFWDHPPHGTRIEAMISPNFHIVKKGETLPAIAARYRVSVNALQSINRLQARARLMPGQKLVIPGTWS
>Mature_476_residues
MMMRAWVLSLLLYLPSSFAVFSAQLKNITVSQQPTMTSLYLSIQGSFTHKLFTLSQPNRVVLDLSDTQLAVNISQLGLIN
SLIQQVRSGSPSKNTLRLVMDVKQPVKVRSSSWKPKGELNGIRIDLVNDTPLVINNRRQLSSPVKTIKAPVQQTQSKISS
QPSEQRITVHKTPVQVPHNPSKKPLRDVVVVLDAGHGGKDPGARGPRRSNEKDVVLAITLKLKRLIDRQPGMRAVLTRSG
DYYVGLRQRLDIARKYNGDVFISIHADAFNNPHSNGASVFALSQRGATSEAARWLAEKENYSELGGVNLGELDDSNGVVR
SVLIDLSQTATINAGLEMGGKVLGQLDNFTNLHNNKVEQARFVVLKSPDIPSILVETGFISNPREERNLTNPAYQERLSQ
AIFQGLKNYFWDHPPHGTRIEAMISPNFHIVKKGETLPAIAARYRVSVNALQSINRLQARARLMPGQKLVIPGTWS

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=370, Percent_Identity=37.8378378378378, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1790611, Length=433, Percent_Identity=35.1039260969977, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788776, Length=225, Percent_Identity=48.4444444444444, Blast_Score=204, Evalue=9e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 52714; Mature: 52714

Theoretical pI: Translated: 10.84; Mature: 10.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMRAWVLSLLLYLPSSFAVFSAQLKNITVSQQPTMTSLYLSIQGSFTHKLFTLSQPNRV
CCHHHHHHHHHHHCCCCHHHHHHHHHCEEECCCCCEEEEEEEEECCEEEEEEEECCCCEE
VLDLSDTQLAVNISQLGLINSLIQQVRSGSPSKNTLRLVMDVKQPVKVRSSSWKPKGELN
EEEECCCEEEEEHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHCCCCCCCCCCCC
GIRIDLVNDTPLVINNRRQLSSPVKTIKAPVQQTQSKISSQPSEQRITVHKTPVQVPHNP
CEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEECCCCC
SKKPLRDVVVVLDAGHGGKDPGARGPRRSNEKDVVLAITLKLKRLIDRQPGMRAVLTRSG
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCEEEEECCC
DYYVGLRQRLDIARKYNGDVFISIHADAFNNPHSNGASVFALSQRGATSEAARWLAEKEN
CEEEEHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCC
YSELGGVNLGELDDSNGVVRSVLIDLSQTATINAGLEMGGKVLGQLDNFTNLHNNKVEQA
HHHHCCCCCCCCCCCCCHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCE
RFVVLKSPDIPSILVETGFISNPREERNLTNPAYQERLSQAIFQGLKNYFWDHPPHGTRI
EEEEEECCCCCEEEEECCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
EAMISPNFHIVKKGETLPAIAARYRVSVNALQSINRLQARARLMPGQKLVIPGTWS
EEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
>Mature Secondary Structure
MMMRAWVLSLLLYLPSSFAVFSAQLKNITVSQQPTMTSLYLSIQGSFTHKLFTLSQPNRV
CCHHHHHHHHHHHCCCCHHHHHHHHHCEEECCCCCEEEEEEEEECCEEEEEEEECCCCEE
VLDLSDTQLAVNISQLGLINSLIQQVRSGSPSKNTLRLVMDVKQPVKVRSSSWKPKGELN
EEEECCCEEEEEHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHCCCCCCCCCCCC
GIRIDLVNDTPLVINNRRQLSSPVKTIKAPVQQTQSKISSQPSEQRITVHKTPVQVPHNP
CEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEECCCCC
SKKPLRDVVVVLDAGHGGKDPGARGPRRSNEKDVVLAITLKLKRLIDRQPGMRAVLTRSG
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCEEEEECCC
DYYVGLRQRLDIARKYNGDVFISIHADAFNNPHSNGASVFALSQRGATSEAARWLAEKEN
CEEEEHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCC
YSELGGVNLGELDDSNGVVRSVLIDLSQTATINAGLEMGGKVLGQLDNFTNLHNNKVEQA
HHHHCCCCCCCCCCCCCHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCE
RFVVLKSPDIPSILVETGFISNPREERNLTNPAYQERLSQAIFQGLKNYFWDHPPHGTRI
EEEEEECCCCCEEEEECCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
EAMISPNFHIVKKGETLPAIAARYRVSVNALQSINRLQARARLMPGQKLVIPGTWS
EEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]