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The map label for this gene is ddl

Identifier: 52842818

GI number: 52842818

Start: 2947732

End: 2948838

Strand: Reverse

Name: ddl

Synonym: lpg2612

Alternate gene names: 52842818

Gene position: 2948838-2947732 (Counterclockwise)

Preceding gene: 52842819

Following gene: 52842817

Centisome position: 86.79

GC content: 38.66

Gene sequence:

>1107_bases
TTGGAGACGCTAATGTCCAAACCCGTTAATTTGGTTTTACTTTATGGTGGAAAATCAGGCGAGCATGAAGTGTCATTGGT
TTCTGCCGCATCGGTTCTAAAACACCTGGATTCCGAGAAATATCATATTATACCCATTGCAATGGATAAAAGTGGTCGAT
TTCATCGTCATGATTATAATGATTTGCTCGCCTGTTCTGATAAATTACCTGTTGTTACTGAAAAATCAACCCCATTGGAA
GGCTTATTAATTAATGGACGTCTGGCAGTTGATGCTAAAATTGTTTTTCCAGTGGTTCATGGCCCTTTATATGAGGATGG
ATGTTTACAAGGTTTACTTGAGTTAGCCGGTGTCGCCTATGTAGGATGTGACGTATTGTCTTCTGCAATCGGAATGGACA
AAGACATGGCGAGGCGACTCGCATGTATTAATGGGCTCAAATCAGCAAGATATAAATTATTATCATGGCATGCCAATGCG
TCTGAAAGACAACAATTTTGTCATGAAGTCGCCTCGGAGTTTGGTTGGCCTCTGTTCGTTAAGCCATGCTCACTAGGTTC
CAGTGTGGGAATTCACAAAGCAAATAATATGGATGAGCTAAACGCAGCAGTTGCTGATGCATTACGCTATGATGAAGAAA
TTCTGGTTGAAGAATTTATTGTCGGAAGGGAAATTGAACTGGCAGTGTTGGAGAACAGTATCCCCTGTGGGAAACCAAGA
GTAAGTATGGTGGGTGAGATAAAAGTAAACCACCCTGACGGTTATTATTCTTATACTGCGAAATATTTGGAAAGCAGTCA
AACGGATTTGATTATTCCAGCTCAATTAAATAATAGTCTGGAAGAACAATTAAAACAGGCTGCAGCGAATATTTTTAGTT
ATCTTAAATGTAAAGGCATGGCCAGGGTAGATTTTTTTGTAAATGATAAGACAGAAGAAATTTACTTTAATGAAATAAAT
ACATTACCTGGATTTACCTCCATAAGTATGTACCCCAAATTGTGGCAGGCAACTGGTATAGCTTATCCTGATTTACTTGA
TGAATTGATTAATCTAGCAATGGTTCATCATAACTGTCGTCAACATTTGGTGACAAATTACTTGTGA

Upstream 100 bases:

>100_bases
GGTACTGCCACGGCAGCTAATATTGAAGCCTTGATTCATTTGGTGCAAACTAAAGTTCGAGAGCAAACATCTATAGAGTT
GATACGCGAAGTACATATCA

Downstream 100 bases:

>100_bases
AGGGAACACTAAATTATGGAAGTGAACTCTGTCGGCTTGCGTTATTTAACGATAATGGGTTTGTTAATTCTTAGTGCTTT
GCTGCTAGCGGGTCGCTTAG

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
METLMSKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAMDKSGRFHRHDYNDLLACSDKLPVVTEKSTPLE
GLLINGRLAVDAKIVFPVVHGPLYEDGCLQGLLELAGVAYVGCDVLSSAIGMDKDMARRLACINGLKSARYKLLSWHANA
SERQQFCHEVASEFGWPLFVKPCSLGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVGREIELAVLENSIPCGKPR
VSMVGEIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSLEEQLKQAAANIFSYLKCKGMARVDFFVNDKTEEIYFNEIN
TLPGFTSISMYPKLWQATGIAYPDLLDELINLAMVHHNCRQHLVTNYL

Sequences:

>Translated_368_residues
METLMSKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAMDKSGRFHRHDYNDLLACSDKLPVVTEKSTPLE
GLLINGRLAVDAKIVFPVVHGPLYEDGCLQGLLELAGVAYVGCDVLSSAIGMDKDMARRLACINGLKSARYKLLSWHANA
SERQQFCHEVASEFGWPLFVKPCSLGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVGREIELAVLENSIPCGKPR
VSMVGEIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSLEEQLKQAAANIFSYLKCKGMARVDFFVNDKTEEIYFNEIN
TLPGFTSISMYPKLWQATGIAYPDLLDELINLAMVHHNCRQHLVTNYL
>Mature_368_residues
METLMSKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAMDKSGRFHRHDYNDLLACSDKLPVVTEKSTPLE
GLLINGRLAVDAKIVFPVVHGPLYEDGCLQGLLELAGVAYVGCDVLSSAIGMDKDMARRLACINGLKSARYKLLSWHANA
SERQQFCHEVASEFGWPLFVKPCSLGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVGREIELAVLENSIPCGKPR
VSMVGEIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSLEEQLKQAAANIFSYLKCKGMARVDFFVNDKTEEIYFNEIN
TLPGFTSISMYPKLWQATGIAYPDLLDELINLAMVHHNCRQHLVTNYL

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=366, Percent_Identity=38.7978142076503, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1786280, Length=354, Percent_Identity=31.3559322033898, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 40897; Mature: 40897

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50975 ATP_GRASP ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLMSKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAMDKSGRFHRHDYN
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCH
DLLACSDKLPVVTEKSTPLEGLLINGRLAVDAKIVFPVVHGPLYEDGCLQGLLELAGVAY
HHHCCCCCCCEEECCCCCCCEEEECCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHH
VGCDVLSSAIGMDKDMARRLACINGLKSARYKLLSWHANASERQQFCHEVASEFGWPLFV
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCEEEE
KPCSLGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVGREIELAVLENSIPCGKPR
EECCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCC
VSMVGEIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSLEEQLKQAAANIFSYLKCKGM
EEEEEEEEEECCCCHHHHHHHHHCCCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCC
ARVDFFVNDKTEEIYFNEINTLPGFTSISMYPKLWQATGIAYPDLLDELINLAMVHHNCR
EEEEEEECCCCCEEEEEHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
QHLVTNYL
HHHHHHCC
>Mature Secondary Structure
METLMSKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAMDKSGRFHRHDYN
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCH
DLLACSDKLPVVTEKSTPLEGLLINGRLAVDAKIVFPVVHGPLYEDGCLQGLLELAGVAY
HHHCCCCCCCEEECCCCCCCEEEECCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHH
VGCDVLSSAIGMDKDMARRLACINGLKSARYKLLSWHANASERQQFCHEVASEFGWPLFV
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCEEEE
KPCSLGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVGREIELAVLENSIPCGKPR
EECCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCC
VSMVGEIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSLEEQLKQAAANIFSYLKCKGM
EEEEEEEEEECCCCHHHHHHHHHCCCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCC
ARVDFFVNDKTEEIYFNEINTLPGFTSISMYPKLWQATGIAYPDLLDELINLAMVHHNCR
EEEEEEECCCCCEEEEEHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
QHLVTNYL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA