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The map label for this gene is purA [H]

Identifier: 52788013

GI number: 52788013

Start: 4188299

End: 4189591

Strand: Reverse

Name: purA [H]

Synonym: BLi04341

Alternate gene names: 52788013

Gene position: 4189591-4188299 (Counterclockwise)

Preceding gene: 52788015

Following gene: 52788012

Centisome position: 99.22

GC content: 49.88

Gene sequence:

>1293_bases
ATGTCTTCAGTAGTTGTTGTAGGTACGCAGTGGGGTGACGAAGGAAAAGGCAAAATCACCGATTTCCTTTCCGAAAATGC
AGAGGTAATCGCACGGTACCAAGGCGGAAATAACGCAGGACATACGATTAAATTCAACGGGGTAACGTACAAACTGCATT
TGATTCCATCCGGTATTTTTTATAAGGATAAAACGTGTGTGATCGGCAACGGAATGGTTGTTGATCCGAAAGCATTGGTA
ACGGAGCTGGCTTATCTTCATGAGCGGAACGTCAGCACGGACAACTTGAGAATCTCCAACAGGGCCCATGTCATTCTGCC
GTATCACTTAAAGCTTGATGCGGTCGAAGAAGAGCGGAAAGGGGCCAATAAAATCGGCACGACGAAAAAAGGAATCGGCC
CGGCTTATATGGATAAAGCCGCTCGCGTCGGAATCCGGATCGCCGATCTGTTGGACAGGGACGTTTTCGAAGAAAAGCTC
GCCCGCAATTTGGAAGAAAAAAACCGTCTGCTAGAAAAAATGTATGATGCGGAAGGATTTAAAATAGAAGACATTTTAGA
CGAATACTATGAGTACGGCCAGCAGATCAAACAATATGTCTGCGATACGTCCGTCGTCTTAAACGACGCACTTGATGACG
GCCGCCGCGTCTTATTCGAAGGGGCGCAAGGAGTTATGCTTGATATCGACCAAGGGACATATCCGTTTGTCACGTCTTCA
AACCCTGTCGCCGGCGGCGTGACGATCGGCTCAGGTGTAGGACCGACAAAAATCCAGCACGTCGTTGGTGTGTCGAAAGC
TTACACAACGCGTGTCGGAGACGGTCCGTTCCCAACAGAGCTGAACAATGAGATCGGAGACCAAATCCGTGAGGTCGGCC
GCGAATACGGAACAACTACAGGTCGGCCGCGCCGCGTCGGCTGGTTTGACAGCGTGGTTGTCCGCCATGCCCGCCGCGTC
AGCGGAATTACCGACCTTTCATTAAATTCAATTGACGTATTGACCGGTATTGAAACATTGAAAATCTGTGTCGCTTACAA
ATACCGCGGCGAGATCCTGGAAGAGTTCCCGGCAAGCTTAAAAGCGCTCGCTGAATGTGAGCCTGTCTATGAAGAAATGC
CGGGTTGGACCGAAGATATCACGGGCGCGAAAAGCTTGAGCGACCTGCCTGAAAATGCCCGCCATTACCTTGAGCGAGTT
TCTCAGCTGACAGGCATTCCGCTTTCCATTTTCTCAGTCGGTCCTGACCGCTCTCAAACAAATGTGGTGAGAAGCGTTTA
TCGTCCGAACTAA

Upstream 100 bases:

>100_bases
TTAATGTTCGGATTTACAATTGACTTTACGGGATGACACTGATAAACTGAATGTGTTTGATATTGATATCGTTTGAAAAG
GTTAACGGAGGTGCACGGAT

Downstream 100 bases:

>100_bases
ATTCTAGAAGAGCCTCTGCAGTCCGCAGGGGCTTTGTTTTTCAATGACAAGGAGGGCAAACGGATGGAACGTGTCGATGT
TGTATATTCATTCGCGGAAG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGNNAGHTIKFNGVTYKLHLIPSGIFYKDKTCVIGNGMVVDPKALV
TELAYLHERNVSTDNLRISNRAHVILPYHLKLDAVEEERKGANKIGTTKKGIGPAYMDKAARVGIRIADLLDRDVFEEKL
ARNLEEKNRLLEKMYDAEGFKIEDILDEYYEYGQQIKQYVCDTSVVLNDALDDGRRVLFEGAQGVMLDIDQGTYPFVTSS
NPVAGGVTIGSGVGPTKIQHVVGVSKAYTTRVGDGPFPTELNNEIGDQIREVGREYGTTTGRPRRVGWFDSVVVRHARRV
SGITDLSLNSIDVLTGIETLKICVAYKYRGEILEEFPASLKALAECEPVYEEMPGWTEDITGAKSLSDLPENARHYLERV
SQLTGIPLSIFSVGPDRSQTNVVRSVYRPN

Sequences:

>Translated_430_residues
MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGNNAGHTIKFNGVTYKLHLIPSGIFYKDKTCVIGNGMVVDPKALV
TELAYLHERNVSTDNLRISNRAHVILPYHLKLDAVEEERKGANKIGTTKKGIGPAYMDKAARVGIRIADLLDRDVFEEKL
ARNLEEKNRLLEKMYDAEGFKIEDILDEYYEYGQQIKQYVCDTSVVLNDALDDGRRVLFEGAQGVMLDIDQGTYPFVTSS
NPVAGGVTIGSGVGPTKIQHVVGVSKAYTTRVGDGPFPTELNNEIGDQIREVGREYGTTTGRPRRVGWFDSVVVRHARRV
SGITDLSLNSIDVLTGIETLKICVAYKYRGEILEEFPASLKALAECEPVYEEMPGWTEDITGAKSLSDLPENARHYLERV
SQLTGIPLSIFSVGPDRSQTNVVRSVYRPN
>Mature_429_residues
SSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGNNAGHTIKFNGVTYKLHLIPSGIFYKDKTCVIGNGMVVDPKALVT
ELAYLHERNVSTDNLRISNRAHVILPYHLKLDAVEEERKGANKIGTTKKGIGPAYMDKAARVGIRIADLLDRDVFEEKLA
RNLEEKNRLLEKMYDAEGFKIEDILDEYYEYGQQIKQYVCDTSVVLNDALDDGRRVLFEGAQGVMLDIDQGTYPFVTSSN
PVAGGVTIGSGVGPTKIQHVVGVSKAYTTRVGDGPFPTELNNEIGDQIREVGREYGTTTGRPRRVGWFDSVVVRHARRVS
GITDLSLNSIDVLTGIETLKICVAYKYRGEILEEFPASLKALAECEPVYEEMPGWTEDITGAKSLSDLPENARHYLERVS
QLTGIPLSIFSVGPDRSQTNVVRSVYRPN

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=426, Percent_Identity=42.4882629107981, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI22748717, Length=421, Percent_Identity=43.7054631828979, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI40316944, Length=392, Percent_Identity=42.3469387755102, Blast_Score=288, Evalue=8e-78,
Organism=Escherichia coli, GI1790620, Length=428, Percent_Identity=47.4299065420561, Blast_Score=410, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25146555, Length=421, Percent_Identity=41.8052256532066, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI25146553, Length=421, Percent_Identity=41.8052256532066, Blast_Score=328, Evalue=3e-90,
Organism=Saccharomyces cerevisiae, GI6324109, Length=425, Percent_Identity=41.1764705882353, Blast_Score=335, Evalue=1e-92,
Organism=Drosophila melanogaster, GI21356033, Length=417, Percent_Identity=42.4460431654676, Blast_Score=337, Evalue=7e-93,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47568; Mature: 47437

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGNNAGHTIKFNGVTYKLHLIPSGIF
CCCEEEEEECCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEECCEEEEEEEEECCCE
YKDKTCVIGNGMVVDPKALVTELAYLHERNVSTDNLRISNRAHVILPYHLKLDAVEEERK
ECCCEEEECCCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEEEEECCHHHHHC
GANKIGTTKKGIGPAYMDKAARVGIRIADLLDRDVFEEKLARNLEEKNRLLEKMYDAEGF
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KIEDILDEYYEYGQQIKQYVCDTSVVLNDALDDGRRVLFEGAQGVMLDIDQGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCEEECC
NPVAGGVTIGSGVGPTKIQHVVGVSKAYTTRVGDGPFPTELNNEIGDQIREVGREYGTTT
CCCCCCEEECCCCCHHHHHHHHHCHHHHEECCCCCCCCCHHCHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVVRHARRVSGITDLSLNSIDVLTGIETLKICVAYKYRGEILEEFPASL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KALAECEPVYEEMPGWTEDITGAKSLSDLPENARHYLERVSQLTGIPLSIFSVGPDRSQT
HHHHHCCHHHHHCCCCCHHCCCHHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHH
NVVRSVYRPN
HHHHHHCCCC
>Mature Secondary Structure 
SSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGNNAGHTIKFNGVTYKLHLIPSGIF
CCEEEEEECCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEECCEEEEEEEEECCCE
YKDKTCVIGNGMVVDPKALVTELAYLHERNVSTDNLRISNRAHVILPYHLKLDAVEEERK
ECCCEEEECCCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEEEEECCHHHHHC
GANKIGTTKKGIGPAYMDKAARVGIRIADLLDRDVFEEKLARNLEEKNRLLEKMYDAEGF
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KIEDILDEYYEYGQQIKQYVCDTSVVLNDALDDGRRVLFEGAQGVMLDIDQGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCEEECC
NPVAGGVTIGSGVGPTKIQHVVGVSKAYTTRVGDGPFPTELNNEIGDQIREVGREYGTTT
CCCCCCEEECCCCCHHHHHHHHHCHHHHEECCCCCCCCCHHCHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVVRHARRVSGITDLSLNSIDVLTGIETLKICVAYKYRGEILEEFPASL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KALAECEPVYEEMPGWTEDITGAKSLSDLPENARHYLERVSQLTGIPLSIFSVGPDRSQT
HHHHHCCHHHHHCCCCCHHCCCHHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHH
NVVRSVYRPN
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA