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The map label for this gene is lacR [H]

Identifier: 52787958

GI number: 52787958

Start: 4125826

End: 4126818

Strand: Reverse

Name: lacR [H]

Synonym: BLi04281

Alternate gene names: 52787958

Gene position: 4126818-4125826 (Counterclockwise)

Preceding gene: 52787962

Following gene: 52787957

Centisome position: 97.73

GC content: 45.52

Gene sequence:

>993_bases
GTGGCGACGATAAAAGATGTTGCAGAAAAGGCGGGGTATTCCATCTCAACGGTTTCCCGCGTATTGAATAACGACGAAAG
CCTTTCCGTACCCGATGAGACGCGGGAGAAAATATTTGAAGTGGCGGAAAGCCTGAACTACCGCAAGAAAACGATCAGGA
CACTGGTAAAAAACATCGCGTTTCTGTACTGGCTGACGGATAAGGAAGAGCTCGAAGACGTGTATTTTAAAACGATGAGG
CTGGAAATCGAGAAGCTCGCAAAGCAATTCAACGTCGAACTGGCAACGTATAAAATTTCGGGAGGCATTGACGCCATTCC
CGAAAACATAGAAGGATTCATTGCCGTCGGCTCGTTTTCAGATGATGAATTAATCCGATTAAGACAGATGACGCCTCACG
GTGTATTTATCGATTCGACGCCTGATCCGAATCACTTTGATTCAGTCAGGCCGGACTTAAAGCAGATGACGAGGAAAACG
GTAGATTTTCTCATTGAGAAAGGGCATGAAAAGATCGGCTTTATCGGAGGTACGTACCACAACCCCAATACAGATGAAGA
CGAAATGGACGTAAGGGAACGAACGTTTCGCGAATATATGAAGGAAAAAGGGCTCTTGGAGGAAAGCTTTATTTTTTGCC
GGAGGGGTTTTTCGGTGGAAAACGGCTACCATCTGATGGACAAAGCAGTTGAAACATTGGGAGAAAAGCTGCCGACGGCC
TTTTTTACAGCGGCGGATCCGATTGCCGTAGGCTGCCTTCAGGCCTTAAATGAAAAAGGGATCGCCATCCCGGGCAGAGT
CAGCATCATCAGCATCAACAACATCAGTGTAGCGAAATACGTATCTCCCCCGCTGACGACATTCCATATCGATATTAAAG
AGCTTTGCAAAAACGCTATTGAATTGCTTTTGGAACAAATACTTCAAAAAAGAAAAGCTGTAAAAACGCTATATATCGGT
GCCGAACTGGTGGTCAGGAAAAGCACCAATTAA

Upstream 100 bases:

>100_bases
AGCACTTGACTTTTACGCAAATCAACGTTAAATTAGTAAAATATTTTACTAAAAAATTTGATATTTAAAATAAAGGTGTT
CATATGGAGGTTTTACAATC

Downstream 100 bases:

>100_bases
AAAAGCGCGGGCTGCTGTTCACAATCCGCGCTTTTTTTCGTAAAACATTTTACCTGTGTAAGTAAAATAATTGTTTACAC
TTTTCCTAAGACATGCTATA

Product: LacR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MATIKDVAEKAGYSISTVSRVLNNDESLSVPDETREKIFEVAESLNYRKKTIRTLVKNIAFLYWLTDKEELEDVYFKTMR
LEIEKLAKQFNVELATYKISGGIDAIPENIEGFIAVGSFSDDELIRLRQMTPHGVFIDSTPDPNHFDSVRPDLKQMTRKT
VDFLIEKGHEKIGFIGGTYHNPNTDEDEMDVRERTFREYMKEKGLLEESFIFCRRGFSVENGYHLMDKAVETLGEKLPTA
FFTAADPIAVGCLQALNEKGIAIPGRVSIISINNISVAKYVSPPLTTFHIDIKELCKNAIELLLEQILQKRKAVKTLYIG
AELVVRKSTN

Sequences:

>Translated_330_residues
MATIKDVAEKAGYSISTVSRVLNNDESLSVPDETREKIFEVAESLNYRKKTIRTLVKNIAFLYWLTDKEELEDVYFKTMR
LEIEKLAKQFNVELATYKISGGIDAIPENIEGFIAVGSFSDDELIRLRQMTPHGVFIDSTPDPNHFDSVRPDLKQMTRKT
VDFLIEKGHEKIGFIGGTYHNPNTDEDEMDVRERTFREYMKEKGLLEESFIFCRRGFSVENGYHLMDKAVETLGEKLPTA
FFTAADPIAVGCLQALNEKGIAIPGRVSIISINNISVAKYVSPPLTTFHIDIKELCKNAIELLLEQILQKRKAVKTLYIG
AELVVRKSTN
>Mature_329_residues
ATIKDVAEKAGYSISTVSRVLNNDESLSVPDETREKIFEVAESLNYRKKTIRTLVKNIAFLYWLTDKEELEDVYFKTMRL
EIEKLAKQFNVELATYKISGGIDAIPENIEGFIAVGSFSDDELIRLRQMTPHGVFIDSTPDPNHFDSVRPDLKQMTRKTV
DFLIEKGHEKIGFIGGTYHNPNTDEDEMDVRERTFREYMKEKGLLEESFIFCRRGFSVENGYHLMDKAVETLGEKLPTAF
FTAADPIAVGCLQALNEKGIAIPGRVSIISINNISVAKYVSPPLTTFHIDIKELCKNAIELLLEQILQKRKAVKTLYIGA
ELVVRKSTN

Specific function: Negatively regulates expression of ganA [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789456, Length=338, Percent_Identity=33.7278106508876, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1788474, Length=320, Percent_Identity=30, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787948, Length=345, Percent_Identity=28.4057971014493, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1790369, Length=342, Percent_Identity=26.6081871345029, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789068, Length=319, Percent_Identity=24.7648902821317, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790194, Length=340, Percent_Identity=26.7647058823529, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1786540, Length=341, Percent_Identity=24.3401759530792, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37453; Mature: 37322

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAEKAGYSISTVSRVLNNDESLSVPDETREKIFEVAESLNYRKKTIRTLVKNIA
CCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FLYWLTDKEELEDVYFKTMRLEIEKLAKQFNVELATYKISGGIDAIPENIEGFIAVGSFS
HEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCEEEECCCC
DDELIRLRQMTPHGVFIDSTPDPNHFDSVRPDLKQMTRKTVDFLIEKGHEKIGFIGGTYH
CHHHHHHHHCCCCCEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
NPNTDEDEMDVRERTFREYMKEKGLLEESFIFCRRGFSVENGYHLMDKAVETLGEKLPTA
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHH
FFTAADPIAVGCLQALNEKGIAIPGRVSIISINNISVAKYVSPPLTTFHIDIKELCKNAI
HHHCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCEEEEHCCCCCCEEEEECHHHHHHHHH
ELLLEQILQKRKAVKTLYIGAELVVRKSTN
HHHHHHHHHHHHHHHHHEECCEEEEEECCC
>Mature Secondary Structure 
ATIKDVAEKAGYSISTVSRVLNNDESLSVPDETREKIFEVAESLNYRKKTIRTLVKNIA
CCHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FLYWLTDKEELEDVYFKTMRLEIEKLAKQFNVELATYKISGGIDAIPENIEGFIAVGSFS
HEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCEEEECCCC
DDELIRLRQMTPHGVFIDSTPDPNHFDSVRPDLKQMTRKTVDFLIEKGHEKIGFIGGTYH
CHHHHHHHHCCCCCEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
NPNTDEDEMDVRERTFREYMKEKGLLEESFIFCRRGFSVENGYHLMDKAVETLGEKLPTA
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHH
FFTAADPIAVGCLQALNEKGIAIPGRVSIISINNISVAKYVSPPLTTFHIDIKELCKNAI
HHHCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCEEEEHCCCCCCEEEEECHHHHHHHHH
ELLLEQILQKRKAVKTLYIGAELVVRKSTN
HHHHHHHHHHHHHHHHHEECCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]