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The map label for this gene is yosT [H]

Identifier: 52787875

GI number: 52787875

Start: 4028015

End: 4028464

Strand: Reverse

Name: yosT [H]

Synonym: BLi04198

Alternate gene names: 52787875

Gene position: 4028464-4028015 (Counterclockwise)

Preceding gene: 52787876

Following gene: 52787874

Centisome position: 95.4

GC content: 39.33

Gene sequence:

>450_bases
ATGAAATTCAAAATCGAAACACTTCCAAACTACCGCATTGCATATATGCGCCGAGTTGGTCCATACGGCCCCGCCAACAT
TGAGGTAATGGAAAGATTAAAAAAATGGGCTAAGAAAAAAGATCTTCTTGAATCCGCCATTCTGTTTGCGATTCCACAAG
ATAACCCGAAAACAACGCCTCCTGATCATTGTCGATTTGATGCTTGTATTGTGATACCGCATGATTATCAAGTCGATGAT
ACCGTTTTTGAAGGCGAAATTTCCGGCGGACAATACCTTATTTACGAGGTTGAACATACAGCAGAAGCTATTCAAAAAGC
ATATTCAGATATTTTCTCATCTCTGCAAAACAAATACCAATTGGACAATAGGCCAATCATGGAAAAATACGTTGGTGATA
TGGTGAACAGTCCGTATTGCGAAATATGTGTGCCCTTAAAATCGGTTTAA

Upstream 100 bases:

>100_bases
AAATTTGCAGAAAATTTTAAAAATATGAATGTAAATATCTTTAAAGCGTGTTACATTCGAAACGGAACGAACAACAATGA
TGGAAAGGAAGAAAGAACCT

Downstream 100 bases:

>100_bases
TTCGTTTTTTTGTGTAGTTGTCACACAAAACTTTGAATCTATAAAAAGAGTTTAACCCGCCGACAGAAAGGGTAAATCAT
AAAATAGCATTCCAGATCAG

Product: YosT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MKFKIETLPNYRIAYMRRVGPYGPANIEVMERLKKWAKKKDLLESAILFAIPQDNPKTTPPDHCRFDACIVIPHDYQVDD
TVFEGEISGGQYLIYEVEHTAEAIQKAYSDIFSSLQNKYQLDNRPIMEKYVGDMVNSPYCEICVPLKSV

Sequences:

>Translated_149_residues
MKFKIETLPNYRIAYMRRVGPYGPANIEVMERLKKWAKKKDLLESAILFAIPQDNPKTTPPDHCRFDACIVIPHDYQVDD
TVFEGEISGGQYLIYEVEHTAEAIQKAYSDIFSSLQNKYQLDNRPIMEKYVGDMVNSPYCEICVPLKSV
>Mature_149_residues
MKFKIETLPNYRIAYMRRVGPYGPANIEVMERLKKWAKKKDLLESAILFAIPQDNPKTTPPDHCRFDACIVIPHDYQVDD
TVFEGEISGGQYLIYEVEHTAEAIQKAYSDIFSSLQNKYQLDNRPIMEKYVGDMVNSPYCEICVPLKSV

Specific function: Inhibits The Supercoiling Activity Of DNA Gyrase. Protects Cells From The DNA Replication Inhibitor Microcin B17 (Mccb17). Blocks Mccb17 Export From Producing Cells. [C]

COG id: COG3449

COG function: function code L; DNA gyrase inhibitor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind [H]

EC number: NA

Molecular weight: Translated: 17198; Mature: 17198

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKIETLPNYRIAYMRRVGPYGPANIEVMERLKKWAKKKDLLESAILFAIPQDNPKTTP
CCEECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCC
PDHCRFDACIVIPHDYQVDDTVFEGEISGGQYLIYEVEHTAEAIQKAYSDIFSSLQNKYQ
CCCCCCCEEEEECCCCCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHC
LDNRPIMEKYVGDMVNSPYCEICVPLKSV
CCCCCHHHHHHHHHHCCCCEEEEECCCCC
>Mature Secondary Structure
MKFKIETLPNYRIAYMRRVGPYGPANIEVMERLKKWAKKKDLLESAILFAIPQDNPKTTP
CCEECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCC
PDHCRFDACIVIPHDYQVDDTVFEGEISGGQYLIYEVEHTAEAIQKAYSDIFSSLQNKYQ
CCCCCCCEEEEECCCCCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHC
LDNRPIMEKYVGDMVNSPYCEICVPLKSV
CCCCCHHHHHHHHHHCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9679200; 9384377 [H]