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The map label for this gene is licH [H]

Identifier: 52787763

GI number: 52787763

Start: 3910758

End: 3912086

Strand: Reverse

Name: licH [H]

Synonym: BLi04086

Alternate gene names: 52787763

Gene position: 3912086-3910758 (Counterclockwise)

Preceding gene: 52787764

Following gene: 52787755

Centisome position: 92.65

GC content: 49.81

Gene sequence:

>1329_bases
ATGAAAAAAGGATTGAAAATCGTAACAATTGGCGGAGGTTCAAGTTATACGCCCGAGCTTGTCGAGGGATTAATCAAACG
GCATGACGAGCTTCCGGTCAGCGAGCTCTGGCTCGTCGATATACCGGAAGGGGAAGAAAAACTTAATATCGTCGGCACGC
TGGCGAAGCGAATGGTCGAAAAAGCAGGCGTCCCGATCAAAGTCCATCTGACGCTAGACCGCAGGGAGGCTTTAAAAGAC
GCTGATTTTGTCACGACGCAATTCCGCGTCGGCTTGCTCGAAGCGAGAGCAAAAGATGAACGAATTCCTTTAAAATACGG
GGTGATCGGTCAGGAAACAAATGGACCGGGCGGCCTTTTCAAGGGGCTTCGCACCATTCCGGTCATCCTTGAGATTGCAA
AAGACATCGAAGAACTGTGCCCCGACGCGTGGCTTGTCAATTTTACCAACCCGGCAGGCATGGTGACTGAAGCGCTGCTC
CGCTATTCTAATTTGAAAAAAGTCGTCGGGCTCTGCAATGTGCCGATCGGCATGAAGATGGGGGTTGCAAAGGCGCTCGA
TGTTGATGAAAGCCGCATAGACATTCAATTTGCAGGATTAAATCATATGGTGTTCGGACTCGACGTTTTCCTTGACGGCG
TCAGCGTCAAAGACAAAGTGATCGAAGCGATGGCAGATCCAGAAAATGCGATGACGATGAAAAATATCTCCGGAGAATCG
TGGGAACCCGACTTTATCCGCGGCCTTGGCCTGATTCCGTGCGGCTACCACCGCTACTACTATAAAACACAGGAAATGCT
TGAACACGAACTGGAGGCTTCAAAAACGGAAGGAACGCGTGCAGAGGTTGTTCAGCAAGTTGAAAAGGAATTGTTTGAGC
TGTATAAAGATCCGGAGCTGGCGATCAAACCGCCCCAGCTTGAAAAGCGCGGCGGCGCATATTACAGTGATGCCGCCTGC
AACTTGATCAGCTCGATTTACAATGACAAGCACGATATTCAACCTGTTAACACGATGAATAACGGAGCGATCGCAAGCAT
TCCTGATGACTCCGCAGTGGAAGTCAACTGTGTCATGACGAAAAACGGGCCGAAACCGATCGCGGTCGGAGATTTGCCTG
TCACCGTGAGAGGTCTTGTCCAGCAAATTAAATCGTTCGAACGCGTTGCGGCTGAAGCGGCTGTTACAGGCGATTACAAC
ACTGCACTTGTGGCCATGACGATCAACCCGCTCGTTCCGTCCGACAAAATTGCGAAACAAATACTGGACGAAATGCTTGA
AGCGCATAAAGAGCATCTTCCGCAGTTTTTTAGATCTGTAGAAGCATAG

Upstream 100 bases:

>100_bases
ATGCCCAGGATCATTTAATGAATGCGATGACCATGAAAGATTTGGCCGCGGAGATTGTCGAGCTCTATGAAAAAATGAAT
ATCCACGGAGGTGTCAATTG

Downstream 100 bases:

>100_bases
GAATAGATTTGAGGTTCCGGTGTGTTTGCCGGAACCTTTTCATTGTTGGAGGCGCCGCTGCGCCCGCCTGACCCGGTGAA
ACTGCGGCCGTCACATTCGT

Product: LicH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MKKGLKIVTIGGGSSYTPELVEGLIKRHDELPVSELWLVDIPEGEEKLNIVGTLAKRMVEKAGVPIKVHLTLDRREALKD
ADFVTTQFRVGLLEARAKDERIPLKYGVIGQETNGPGGLFKGLRTIPVILEIAKDIEELCPDAWLVNFTNPAGMVTEALL
RYSNLKKVVGLCNVPIGMKMGVAKALDVDESRIDIQFAGLNHMVFGLDVFLDGVSVKDKVIEAMADPENAMTMKNISGES
WEPDFIRGLGLIPCGYHRYYYKTQEMLEHELEASKTEGTRAEVVQQVEKELFELYKDPELAIKPPQLEKRGGAYYSDAAC
NLISSIYNDKHDIQPVNTMNNGAIASIPDDSAVEVNCVMTKNGPKPIAVGDLPVTVRGLVQQIKSFERVAAEAAVTGDYN
TALVAMTINPLVPSDKIAKQILDEMLEAHKEHLPQFFRSVEA

Sequences:

>Translated_442_residues
MKKGLKIVTIGGGSSYTPELVEGLIKRHDELPVSELWLVDIPEGEEKLNIVGTLAKRMVEKAGVPIKVHLTLDRREALKD
ADFVTTQFRVGLLEARAKDERIPLKYGVIGQETNGPGGLFKGLRTIPVILEIAKDIEELCPDAWLVNFTNPAGMVTEALL
RYSNLKKVVGLCNVPIGMKMGVAKALDVDESRIDIQFAGLNHMVFGLDVFLDGVSVKDKVIEAMADPENAMTMKNISGES
WEPDFIRGLGLIPCGYHRYYYKTQEMLEHELEASKTEGTRAEVVQQVEKELFELYKDPELAIKPPQLEKRGGAYYSDAAC
NLISSIYNDKHDIQPVNTMNNGAIASIPDDSAVEVNCVMTKNGPKPIAVGDLPVTVRGLVQQIKSFERVAAEAAVTGDYN
TALVAMTINPLVPSDKIAKQILDEMLEAHKEHLPQFFRSVEA
>Mature_442_residues
MKKGLKIVTIGGGSSYTPELVEGLIKRHDELPVSELWLVDIPEGEEKLNIVGTLAKRMVEKAGVPIKVHLTLDRREALKD
ADFVTTQFRVGLLEARAKDERIPLKYGVIGQETNGPGGLFKGLRTIPVILEIAKDIEELCPDAWLVNFTNPAGMVTEALL
RYSNLKKVVGLCNVPIGMKMGVAKALDVDESRIDIQFAGLNHMVFGLDVFLDGVSVKDKVIEAMADPENAMTMKNISGES
WEPDFIRGLGLIPCGYHRYYYKTQEMLEHELEASKTEGTRAEVVQQVEKELFELYKDPELAIKPPQLEKRGGAYYSDAAC
NLISSIYNDKHDIQPVNTMNNGAIASIPDDSAVEVNCVMTKNGPKPIAVGDLPVTVRGLVQQIKSFERVAAEAAVTGDYN
TALVAMTINPLVPSDKIAKQILDEMLEAHKEHLPQFFRSVEA

Specific function: Hydrolyzes phospho-beta-glucosides (Probable) [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=439, Percent_Identity=54.2141230068337, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1790560, Length=467, Percent_Identity=28.4796573875803, Blast_Score=154, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 48742; Mature: 48742

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGLKIVTIGGGSSYTPELVEGLIKRHDELPVSELWLVDIPEGEEKLNIVGTLAKRMVE
CCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHH
KAGVPIKVHLTLDRREALKDADFVTTQFRVGLLEARAKDERIPLKYGVIGQETNGPGGLF
HCCCCEEEEEEECHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEECEEECCCCCCHHHH
KGLRTIPVILEIAKDIEELCPDAWLVNFTNPAGMVTEALLRYSNLKKVVGLCNVPIGMKM
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
GVAKALDVDESRIDIQFAGLNHMVFGLDVFLDGVSVKDKVIEAMADPENAMTMKNISGES
CCHHHCCCCCCEEEEEEECCCHHHEEHHHHHCCCCHHHHHHHHHCCCCHHEEECCCCCCC
WEPDFIRGLGLIPCGYHRYYYKTQEMLEHELEASKTEGTRAEVVQQVEKELFELYKDPEL
CCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
AIKPPQLEKRGGAYYSDAACNLISSIYNDKHDIQPVNTMNNGAIASIPDDSAVEVNCVMT
EECCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEEE
KNGPKPIAVGDLPVTVRGLVQQIKSFERVAAEAAVTGDYNTALVAMTINPLVPSDKIAKQ
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHH
ILDEMLEAHKEHLPQFFRSVEA
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKGLKIVTIGGGSSYTPELVEGLIKRHDELPVSELWLVDIPEGEEKLNIVGTLAKRMVE
CCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHH
KAGVPIKVHLTLDRREALKDADFVTTQFRVGLLEARAKDERIPLKYGVIGQETNGPGGLF
HCCCCEEEEEEECHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEECEEECCCCCCHHHH
KGLRTIPVILEIAKDIEELCPDAWLVNFTNPAGMVTEALLRYSNLKKVVGLCNVPIGMKM
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
GVAKALDVDESRIDIQFAGLNHMVFGLDVFLDGVSVKDKVIEAMADPENAMTMKNISGES
CCHHHCCCCCCEEEEEEECCCHHHEEHHHHHCCCCHHHHHHHHHCCCCHHEEECCCCCCC
WEPDFIRGLGLIPCGYHRYYYKTQEMLEHELEASKTEGTRAEVVQQVEKELFELYKDPEL
CCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
AIKPPQLEKRGGAYYSDAACNLISSIYNDKHDIQPVNTMNNGAIASIPDDSAVEVNCVMT
EECCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEEE
KNGPKPIAVGDLPVTVRGLVQQIKSFERVAAEAAVTGDYNTALVAMTINPLVPSDKIAKQ
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHH
ILDEMLEAHKEHLPQFFRSVEA
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990303; 9384377; 8969509 [H]