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The map label for this gene is mscL

Identifier: 52787551

GI number: 52787551

Start: 3696680

End: 3697060

Strand: Reverse

Name: mscL

Synonym: BLi03872

Alternate gene names: 52787551

Gene position: 3697060-3696680 (Counterclockwise)

Preceding gene: 52787552

Following gene: 52787549

Centisome position: 87.55

GC content: 40.42

Gene sequence:

>381_bases
ATGTGGAAAGAATTTAAAAGTTTTGCGATTCGGGGAAATGTAATCGATCTTGCAATTGGTGTCATCATCGGAGGCGCCTT
TGGAAAAATCGTAACTTCGCTTGTCAATGATTTGATGATGCCGCTCCTCGGTCTTCTGCTTGGAGGCCTGGATTTTTCCG
CGCTGTCGTTCACATTTGTTGATGCCGAAATTAAATATGGGCTATTCATTCAGTCGATCGTCAATTTCTTTATTATTTCG
TTTTCCATTTTTCTATTTATCCGCTATATCTCTAAGCTGAAGAAGAAGGATGCCGAAGAAGAAAAAGCTGCTCCTGATCC
TCAGGAAGAATTGCTTAAGGAAATACGGGATTTGTTAAAAGAGCAGACAAACCGTTCTTAG

Upstream 100 bases:

>100_bases
GATGTTTTCTCTTGGTGAAAAGCAGGAATAATGTAAACACAATGGGTTTTCGTAAAAGCCCATTTGTTTTTTTATCATGA
AAGCGAAAAGGTGATCATTC

Downstream 100 bases:

>100_bases
AGCTATAAATGAAAAGGCTGTCCGGCGAAGACAGCCTTTTTTATATATTTTGCTTAAATGCTTCAAGAAGCCGATCTCCG
GAATATCCTTCTTTCATTAA

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MWKEFKSFAIRGNVIDLAIGVIIGGAFGKIVTSLVNDLMMPLLGLLLGGLDFSALSFTFVDAEIKYGLFIQSIVNFFIIS
FSIFLFIRYISKLKKKDAEEEKAAPDPQEELLKEIRDLLKEQTNRS

Sequences:

>Translated_126_residues
MWKEFKSFAIRGNVIDLAIGVIIGGAFGKIVTSLVNDLMMPLLGLLLGGLDFSALSFTFVDAEIKYGLFIQSIVNFFIIS
FSIFLFIRYISKLKKKDAEEEKAAPDPQEELLKEIRDLLKEQTNRS
>Mature_126_residues
MWKEFKSFAIRGNVIDLAIGVIIGGAFGKIVTSLVNDLMMPLLGLLLGGLDFSALSFTFVDAEIKYGLFIQSIVNFFIIS
FSIFLFIRYISKLKKKDAEEEKAAPDPQEELLKEIRDLLKEQTNRS

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=133, Percent_Identity=57.1428571428571, Blast_Score=147, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_BACLD (Q65E27)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080950.1
- RefSeq:   YP_093380.1
- ProteinModelPortal:   Q65E27
- SMR:   Q65E27
- STRING:   Q65E27
- EnsemblBacteria:   EBBACT00000056733
- EnsemblBacteria:   EBBACT00000061885
- GeneID:   3028707
- GeneID:   3101083
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03872
- KEGG:   bli:BL02495
- NMPDR:   fig|279010.5.peg.4064
- eggNOG:   COG1970
- GeneTree:   EBGT00050000002096
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- ProtClustDB:   PRK13955
- BioCyc:   BLIC279010-1:BLI03872-MONOMER
- BioCyc:   BLIC279010:BL02495-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 14210; Mature: 14210

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12f309dc)-; HASH(0x12b7d1e4)-; HASH(0x12e3775c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWKEFKSFAIRGNVIDLAIGVIIGGAFGKIVTSLVNDLMMPLLGLLLGGLDFSALSFTFV
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DAEIKYGLFIQSIVNFFIISFSIFLFIRYISKLKKKDAEEEKAAPDPQEELLKEIRDLLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHH
EQTNRS
HHCCCH
>Mature Secondary Structure
MWKEFKSFAIRGNVIDLAIGVIIGGAFGKIVTSLVNDLMMPLLGLLLGGLDFSALSFTFV
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DAEIKYGLFIQSIVNFFIISFSIFLFIRYISKLKKKDAEEEKAAPDPQEELLKEIRDLLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHH
EQTNRS
HHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA