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The map label for this gene is kdgA [H]

Identifier: 52787506

GI number: 52787506

Start: 3653084

End: 3653698

Strand: Reverse

Name: kdgA [H]

Synonym: BLi03826

Alternate gene names: 52787506

Gene position: 3653698-3653084 (Counterclockwise)

Preceding gene: 52787507

Following gene: 52787505

Centisome position: 86.53

GC content: 53.33

Gene sequence:

>615_bases
ATGGAAATGTCTATGATCGGAAAAGTGCAAGAGCAGCTGGGCGAGGCCGGGCTGATCGCAGTCGTCCGCGCAGACGGTAA
GGAGGCTGCTGAGGAGACCATTCACAGACTGATCGACAAAGGGATTACTGCGATTGAAATCACATATACGACGCCGGGAG
CGTCTGAGCTGATTGAAAAATTTGCGGGAAAAGACGGTCTTCTTGTCGGAGCGGGAACGGTGGTTGAAAGCGAACAGGCG
AGGGAAGCCGCGGCAGCGGGGTCAAAATTTATCGTCAGCCCGGGTTTTTCGGAATCACTGGCTGAGGAGGCCGCATCATT
GCATACATTTTTTATTCCCGGCGTATTAACGCCAAGCGAAATTATGGAAGCGGTCTCAAAGGGTTTTCGGGTTCTTAAGC
TGTTTCCAGGGGGAACATCCGGGATTCCTTACATGAAGAACCTCGCCGGTCCTTTTCCGAAGGTTCGGTTCATACCAACC
GGAGGAATCCATCCCGGCGATGTGGAGAAGTGGCTTGATGCCGGAGCATTGGCAGTAGGTGTGGGAAGCCAGCTGAACAA
AGTGTCAGAAGCGGATCTCCAGCGCATCTTTCAGCGGCGGGCTCAGCAGAAATAA

Upstream 100 bases:

>100_bases
TCATGGCTCCGGGGGATATGGACGGTCTGCCGTCAAGGGATCAATTGAAAGCTTTTATGGCTGGAGAGAAAAACAACCAA
AAAATAGGAGGTTGACCGTA

Downstream 100 bases:

>100_bases
AGCGTGCGCAGCGTGGTAAAGACTTCAGGTTTTGCAAGATCGACAGCTTTGCTTTTTCATTAATTTCTAGAATGGGAGAA
AAAAATGATGAAAATTAAAG

Product: KdgA

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MEMSMIGKVQEQLGEAGLIAVVRADGKEAAEETIHRLIDKGITAIEITYTTPGASELIEKFAGKDGLLVGAGTVVESEQA
REAAAAGSKFIVSPGFSESLAEEAASLHTFFIPGVLTPSEIMEAVSKGFRVLKLFPGGTSGIPYMKNLAGPFPKVRFIPT
GGIHPGDVEKWLDAGALAVGVGSQLNKVSEADLQRIFQRRAQQK

Sequences:

>Translated_204_residues
MEMSMIGKVQEQLGEAGLIAVVRADGKEAAEETIHRLIDKGITAIEITYTTPGASELIEKFAGKDGLLVGAGTVVESEQA
REAAAAGSKFIVSPGFSESLAEEAASLHTFFIPGVLTPSEIMEAVSKGFRVLKLFPGGTSGIPYMKNLAGPFPKVRFIPT
GGIHPGDVEKWLDAGALAVGVGSQLNKVSEADLQRIFQRRAQQK
>Mature_204_residues
MEMSMIGKVQEQLGEAGLIAVVRADGKEAAEETIHRLIDKGITAIEITYTTPGASELIEKFAGKDGLLVGAGTVVESEQA
REAAAAGSKFIVSPGFSESLAEEAASLHTFFIPGVLTPSEIMEAVSKGFRVLKLFPGGTSGIPYMKNLAGPFPKVRFIPT
GGIHPGDVEKWLDAGALAVGVGSQLNKVSEADLQRIFQRRAQQK

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=165, Percent_Identity=34.5454545454545, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI48994954, Length=189, Percent_Identity=31.2169312169312, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 21567; Mature: 21567

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMSMIGKVQEQLGEAGLIAVVRADGKEAAEETIHRLIDKGITAIEITYTTPGASELIEK
CCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHH
FAGKDGLLVGAGTVVESEQAREAAAAGSKFIVSPGFSESLAEEAASLHTFFIPGVLTPSE
HCCCCCEEEECCCHHCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHH
IMEAVSKGFRVLKLFPGGTSGIPYMKNLAGPFPKVRFIPTGGIHPGDVEKWLDAGALAVG
HHHHHHCCCEEEEECCCCCCCCCHHHHHCCCCCCEEEEECCCCCCCHHHHHHHCCHHEEC
VGSQLNKVSEADLQRIFQRRAQQK
CCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEMSMIGKVQEQLGEAGLIAVVRADGKEAAEETIHRLIDKGITAIEITYTTPGASELIEK
CCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHH
FAGKDGLLVGAGTVVESEQAREAAAAGSKFIVSPGFSESLAEEAASLHTFFIPGVLTPSE
HCCCCCEEEECCCHHCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHH
IMEAVSKGFRVLKLFPGGTSGIPYMKNLAGPFPKVRFIPTGGIHPGDVEKWLDAGALAVG
HHHHHHCCCEEEEECCCCCCCCCHHHHHCCCCCCEEEEECCCCCCCHHHHHHHCCHHEEC
VGSQLNKVSEADLQRIFQRRAQQK
CCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]