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The map label for this gene is hisZ

Identifier: 52787421

GI number: 52787421

Start: 3561253

End: 3562428

Strand: Reverse

Name: hisZ

Synonym: BLi03738

Alternate gene names: 52787421

Gene position: 3562428-3561253 (Counterclockwise)

Preceding gene: 52787424

Following gene: 52787420

Centisome position: 84.36

GC content: 48.55

Gene sequence:

>1176_bases
ATGTTTATGTTTGAAAAACCGCATGGCATGCGGGATACGCTGCCTATGCTTTACGAAACAAAGAAAAAGGTGCGCTCTTC
ATTAACAGAGGTGATGGCTTTGTGGGGGTATCAGTTCATGGAGACGCCTGCCCTTGAATTTTATGATACGGTCGGTGTGC
AGTCGGCCATATTGGATCAGCAGTTGTTTAAACTCCTTGATCAGGAGGGGCAGACGCTCGTGTTAAGGCCTGATATGACG
GCTCCAATCGCCAGGGTGGCGGCCTCAAAGCTTCATAAAGACGATCATCCGCTCAGAGTAGGCTATGCTGCAAATGTGTT
CCGGGCACAGGAGCGTGAAGGAGGACGCCCCGCCGAATTTGAGCAAGTCGGCGTTGAACTGATCGGCGATGGATCGACAA
GCGCTGACGCAGAAGTGATTGCGCTCGTCATTTTTTCGCTTAAAAATGCCGGCCTGACTTCATTTAAAATCTCGATCGGC
CATGTCGGTATTTTAGACGCCTTGTTTGTGGAAGTTTTAGGGAATGAAGAAAGGGCTCATGTCCTGCGGCGCTATTTATA
CGAAAAGAACTATGTCGGCTATCGGGAGCATGTAAAATCCCTCAATTTATCCTCAATTGATAAAAGCAGGCTGCTGGAGC
TTTTGGAGCTGAGGGGCGGTATTGAAACGTGCAGCAGGGCGGCATCCATCGTCGATTCTGAAAAAGGAAAACGTGCGGTT
TTAGAACTTGAAACGCTATGGGAGACGCTCGGCGATTATGGTTGCACAGACGACATTAAGCTCGATTTAGGCATGGTCAG
CCATATGAGCTACTATACCGGTGTTTTGTTTGAAATCTATGCTGAAAACGTCGGCTTTCCGATCGGAAACGGCGGCAGAT
ATGATCAGCTTCTCGGCCGCTTCGACTCACCCGCACCGGCAACCGGTTTCGGCATCAGGACTGACAGGCTGCTGGAAGCA
TTGAAGCCGGCGGAAGAACAAGAAAAGATCGATGTCGTCATCTTCAGCACAGAACAGAGAAAAGAGGCAATCAGATTTGC
CGAAGAAGAACGAAGAAAAGGCAAAAAAGTCGTCATGCAGGACTTAGCGGGAATCGGAGATATTGATCAAATGACAAAGT
CTTTTCAAAACGTGACCTATTTTATCGGAGCCAGAAAGGAAGAGAAGCATGGGTAA

Upstream 100 bases:

>100_bases
GCTGTTGATTTTGATTCTTTTATCTGTTAGCATATTATCATATTAGCGAACTAAAGTATTCGGAATTTTCCTTCAAACGG
TTATGGGGGTGCAAGTGTAA

Downstream 100 bases:

>100_bases
GGAATTGACGATTGCGATGCCGAAAGGGCGGATATTTGAGGAAGCGGCCGATATGCTTCGAAAAGCAGGCTATCAGCTCC
CGGAAGAATTCGACGATTCG

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MFMFEKPHGMRDTLPMLYETKKKVRSSLTEVMALWGYQFMETPALEFYDTVGVQSAILDQQLFKLLDQEGQTLVLRPDMT
APIARVAASKLHKDDHPLRVGYAANVFRAQEREGGRPAEFEQVGVELIGDGSTSADAEVIALVIFSLKNAGLTSFKISIG
HVGILDALFVEVLGNEERAHVLRRYLYEKNYVGYREHVKSLNLSSIDKSRLLELLELRGGIETCSRAASIVDSEKGKRAV
LELETLWETLGDYGCTDDIKLDLGMVSHMSYYTGVLFEIYAENVGFPIGNGGRYDQLLGRFDSPAPATGFGIRTDRLLEA
LKPAEEQEKIDVVIFSTEQRKEAIRFAEEERRKGKKVVMQDLAGIGDIDQMTKSFQNVTYFIGARKEEKHG

Sequences:

>Translated_391_residues
MFMFEKPHGMRDTLPMLYETKKKVRSSLTEVMALWGYQFMETPALEFYDTVGVQSAILDQQLFKLLDQEGQTLVLRPDMT
APIARVAASKLHKDDHPLRVGYAANVFRAQEREGGRPAEFEQVGVELIGDGSTSADAEVIALVIFSLKNAGLTSFKISIG
HVGILDALFVEVLGNEERAHVLRRYLYEKNYVGYREHVKSLNLSSIDKSRLLELLELRGGIETCSRAASIVDSEKGKRAV
LELETLWETLGDYGCTDDIKLDLGMVSHMSYYTGVLFEIYAENVGFPIGNGGRYDQLLGRFDSPAPATGFGIRTDRLLEA
LKPAEEQEKIDVVIFSTEQRKEAIRFAEEERRKGKKVVMQDLAGIGDIDQMTKSFQNVTYFIGARKEEKHG
>Mature_391_residues
MFMFEKPHGMRDTLPMLYETKKKVRSSLTEVMALWGYQFMETPALEFYDTVGVQSAILDQQLFKLLDQEGQTLVLRPDMT
APIARVAASKLHKDDHPLRVGYAANVFRAQEREGGRPAEFEQVGVELIGDGSTSADAEVIALVIFSLKNAGLTSFKISIG
HVGILDALFVEVLGNEERAHVLRRYLYEKNYVGYREHVKSLNLSSIDKSRLLELLELRGGIETCSRAASIVDSEKGKRAV
LELETLWETLGDYGCTDDIKLDLGMVSHMSYYTGVLFEIYAENVGFPIGNGGRYDQLLGRFDSPAPATGFGIRTDRLLEA
LKPAEEQEKIDVVIFSTEQRKEAIRFAEEERRKGKKVVMQDLAGIGDIDQMTKSFQNVTYFIGARKEEKHG

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

Organism=Homo sapiens, GI15029520, Length=342, Percent_Identity=25.1461988304094, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI6996014, Length=372, Percent_Identity=25, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1788861, Length=343, Percent_Identity=23.6151603498542, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71993686, Length=384, Percent_Identity=25.78125, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71993693, Length=381, Percent_Identity=25.7217847769029, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325290, Length=361, Percent_Identity=21.606648199446, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24643061, Length=361, Percent_Identity=23.8227146814404, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_BACLD (Q65EF7)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080824.2
- RefSeq:   YP_093250.1
- ProteinModelPortal:   Q65EF7
- SMR:   Q65EF7
- STRING:   Q65EF7
- EnsemblBacteria:   EBBACT00000056824
- EnsemblBacteria:   EBBACT00000061980
- GeneID:   3028796
- GeneID:   3100935
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03738
- KEGG:   bli:BL03406
- NMPDR:   fig|279010.5.peg.3803
- eggNOG:   COG3705
- GeneTree:   EBGT00050000001169
- HOGENOM:   HBG616575
- ProtClustDB:   PRK12292
- BioCyc:   BLIC279010-1:BLI03738-MONOMER
- BioCyc:   BLIC279010:BL03406-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: PF00587 tRNA-synt_2b

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 43907; Mature: 43907

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMFEKPHGMRDTLPMLYETKKKVRSSLTEVMALWGYQFMETPALEFYDTVGVQSAILDQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QLFKLLDQEGQTLVLRPDMTAPIARVAASKLHKDDHPLRVGYAANVFRAQEREGGRPAEF
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHCCCCCCHH
EQVGVELIGDGSTSADAEVIALVIFSLKNAGLTSFKISIGHVGILDALFVEVLGNEERAH
HHCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHH
VLRRYLYEKNYVGYREHVKSLNLSSIDKSRLLELLELRGGIETCSRAASIVDSEKGKRAV
HHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHH
LELETLWETLGDYGCTDDIKLDLGMVSHMSYYTGVLFEIYAENVGFPIGNGGRYDQLLGR
HHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHC
FDSPAPATGFGIRTDRLLEALKPAEEQEKIDVVIFSTEQRKEAIRFAEEERRKGKKVVMQ
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
DLAGIGDIDQMTKSFQNVTYFIGARKEEKHG
HHCCCCCHHHHHHHHHHEEEEEECCCCCCCC
>Mature Secondary Structure
MFMFEKPHGMRDTLPMLYETKKKVRSSLTEVMALWGYQFMETPALEFYDTVGVQSAILDQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QLFKLLDQEGQTLVLRPDMTAPIARVAASKLHKDDHPLRVGYAANVFRAQEREGGRPAEF
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHCCCCCCHH
EQVGVELIGDGSTSADAEVIALVIFSLKNAGLTSFKISIGHVGILDALFVEVLGNEERAH
HHCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHH
VLRRYLYEKNYVGYREHVKSLNLSSIDKSRLLELLELRGGIETCSRAASIVDSEKGKRAV
HHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHH
LELETLWETLGDYGCTDDIKLDLGMVSHMSYYTGVLFEIYAENVGFPIGNGGRYDQLLGR
HHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHC
FDSPAPATGFGIRTDRLLEALKPAEEQEKIDVVIFSTEQRKEAIRFAEEERRKGKKVVMQ
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
DLAGIGDIDQMTKSFQNVTYFIGARKEEKHG
HHCCCCCHHHHHHHHHHEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA