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The map label for this gene is hisF

Identifier: 52787415

GI number: 52787415

Start: 3556630

End: 3557388

Strand: Reverse

Name: hisF

Synonym: BLi03732

Alternate gene names: 52787415

Gene position: 3557388-3556630 (Counterclockwise)

Preceding gene: 52787416

Following gene: 52787414

Centisome position: 84.25

GC content: 50.72

Gene sequence:

>759_bases
GTGATCACAAAACGAATCATCCCGTGTTTGGACGTAAAAGATGGACGCGTTGTCAAAGGTGTACAATTTGTTGAATTGAA
AGATGCCGGCGACCCGGTTGAACTGGCTGAAGTATATGATCATGAAGGCGCAGATGAGCTTGTTTTTCTCGATATCTCGG
CTTCCCACGAGGGGAGAAAAACGATGGTTGATGTCGTTGAAAGAGTGGCGGCCAAGCTGGCGATCCCGTTTACCGTCGGC
GGCGGCATCAATCGTTTGGATGATATGAAAACGATACTGCGGGCCGGAGCCGACAAAGTTTCCGTCAATACGGCTGCCGT
TCTCCGGCCGGAACTGATAACGGAAGGCGCCGACTTTTTCGGCTCTCAATGCATTGTCGTCGCGATTGATGCAAAATACG
ATCCAGAAGCTGATCTGTACTATGTTTATACACACGGCGGTCGGAAAAAAACGAACCTTGAGGCCGTTTCCTGGGCGAAG
GAAGCCGTTCGGCGGGGAGCAGGTGAAATCCTGCTGACAAGCATGGATTCAGACGGAGAAAAGAACGGATTTGACTGCAG
GCTGACAAAGCTTGTGTCAGAAGCCGTTTCAGTCCCGGTCATTGCATCCGGAGGCGCAGGGCGTGCGGACCACATGTATG
ATGCGTTTGCAGAAGGCCGGGCGGACGCGGCTCTTGCCGCATCTATCTTTCATTACAAAGAAACATCTGTAAAAGAAGTA
AAAGCTTATTTAAAAGAGCGCGGGGTGAATGTAAGATGA

Upstream 100 bases:

>100_bases
CTTCCCGCGCTTCGGAAGGCGTCTCTGGCGCTATTGTCGGAAAAGCTCTTTATACCAACCAATTTACCGTGGCTGAAGCG
CTTGAAAGGGTGGCGGCAAA

Downstream 100 bases:

>100_bases
TCAAGGCAGACGAGTTGACATTTAATGAAGCCGGCTTGATCCCGGCGATTGTCCAGGATGCAGCGAGCAAAGAAGTGCTG
ACACTTGCTTATATGAATAA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MITKRIIPCLDVKDGRVVKGVQFVELKDAGDPVELAEVYDHEGADELVFLDISASHEGRKTMVDVVERVAAKLAIPFTVG
GGINRLDDMKTILRAGADKVSVNTAAVLRPELITEGADFFGSQCIVVAIDAKYDPEADLYYVYTHGGRKKTNLEAVSWAK
EAVRRGAGEILLTSMDSDGEKNGFDCRLTKLVSEAVSVPVIASGGAGRADHMYDAFAEGRADAALAASIFHYKETSVKEV
KAYLKERGVNVR

Sequences:

>Translated_252_residues
MITKRIIPCLDVKDGRVVKGVQFVELKDAGDPVELAEVYDHEGADELVFLDISASHEGRKTMVDVVERVAAKLAIPFTVG
GGINRLDDMKTILRAGADKVSVNTAAVLRPELITEGADFFGSQCIVVAIDAKYDPEADLYYVYTHGGRKKTNLEAVSWAK
EAVRRGAGEILLTSMDSDGEKNGFDCRLTKLVSEAVSVPVIASGGAGRADHMYDAFAEGRADAALAASIFHYKETSVKEV
KAYLKERGVNVR
>Mature_252_residues
MITKRIIPCLDVKDGRVVKGVQFVELKDAGDPVELAEVYDHEGADELVFLDISASHEGRKTMVDVVERVAAKLAIPFTVG
GGINRLDDMKTILRAGADKVSVNTAAVLRPELITEGADFFGSQCIVVAIDAKYDPEADLYYVYTHGGRKKTNLEAVSWAK
EAVRRGAGEILLTSMDSDGEKNGFDCRLTKLVSEAVSVPVIASGGAGRADHMYDAFAEGRADAALAASIFHYKETSVKEV
KAYLKERGVNVR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=50.78125, Blast_Score=244, Evalue=3e-66,
Organism=Escherichia coli, GI87082028, Length=209, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=33.8658146964856, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_BACLD (Q65EG3)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080818.1
- RefSeq:   YP_093244.1
- ProteinModelPortal:   Q65EG3
- SMR:   Q65EG3
- STRING:   Q65EG3
- EnsemblBacteria:   EBBACT00000055954
- EnsemblBacteria:   EBBACT00000062030
- GeneID:   3028642
- GeneID:   3100874
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03732
- KEGG:   bli:BL03412
- NMPDR:   fig|279010.5.peg.3797
- eggNOG:   COG0107
- GeneTree:   EBGT00050000001074
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   BLIC279010-1:BLI03732-MONOMER
- BioCyc:   BLIC279010:BL03412-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27266; Mature: 27266

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITKRIIPCLDVKDGRVVKGVQFVELKDAGDPVELAEVYDHEGADELVFLDISASHEGRK
CCCCCCCCCCCCCCCCEECCEEEEEEECCCCCHHHHHHHCCCCCCCEEEEEECCCCCCHH
TMVDVVERVAAKLAIPFTVGGGINRLDDMKTILRAGADKVSVNTAAVLRPELITEGADFF
HHHHHHHHHHHHEECEEEECCCCCHHHHHHHHHHCCCCEEECCHHHHCCHHHHHCCHHHC
GSQCIVVAIDAKYDPEADLYYVYTHGGRKKTNLEAVSWAKEAVRRGAGEILLTSMDSDGE
CCCEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCC
KNGFDCRLTKLVSEAVSVPVIASGGAGRADHMYDAFAEGRADAALAASIFHYKETSVKEV
CCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHH
KAYLKERGVNVR
HHHHHHCCCCCC
>Mature Secondary Structure
MITKRIIPCLDVKDGRVVKGVQFVELKDAGDPVELAEVYDHEGADELVFLDISASHEGRK
CCCCCCCCCCCCCCCCEECCEEEEEEECCCCCHHHHHHHCCCCCCCEEEEEECCCCCCHH
TMVDVVERVAAKLAIPFTVGGGINRLDDMKTILRAGADKVSVNTAAVLRPELITEGADFF
HHHHHHHHHHHHEECEEEECCCCCHHHHHHHHHHCCCCEEECCHHHHCCHHHHHCCHHHC
GSQCIVVAIDAKYDPEADLYYVYTHGGRKKTNLEAVSWAKEAVRRGAGEILLTSMDSDGE
CCCEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCC
KNGFDCRLTKLVSEAVSVPVIASGGAGRADHMYDAFAEGRADAALAASIFHYKETSVKEV
CCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHH
KAYLKERGVNVR
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA