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The map label for this gene is sigL [H]

Identifier: 52787362

GI number: 52787362

Start: 3505290

End: 3506603

Strand: Reverse

Name: sigL [H]

Synonym: BLi03678

Alternate gene names: 52787362

Gene position: 3506603-3505290 (Counterclockwise)

Preceding gene: 52787364

Following gene: 52787360

Centisome position: 83.04

GC content: 49.09

Gene sequence:

>1314_bases
ATGATGAACTTGCATGTACAGCAGCAGCAAACGGCAAAACTTCACTTGACGCAAGGGCTTGCCCAAGCCATTCATATGCT
TCAGTATTCGTCGGCCGAGCTGAACAGCCGGATTGATGAACTCTCATTGGAAAACCCCCTGATCGAACGAAAAGACTCCG
ATATTCCCCGATCATTTTACCATAAAACGAATCGTCATTTGCAAAATGAAGGAATAAGCCGCTCTGAGACGGCCCATTCC
CGTGAAGATGTTAAAAGCGCTTTGAAACGGCAGGCGCTCGATCTCCAGCTGTCAAGCCGCGAAAAGCGGGTTTTCAGCTA
TTTGGTCGAATCACTTGATCCAAACGGATATGTGACAGAAGACATCGCCTTTATCGCTTCCGAGCTGGCTGTCACCAGAG
AAGAAGCTGAAGAAGTCTTGGGCAAGCTGCAGACTCTTGATCCGGCAGGCATCGGGGCGCGGTCGCTACAGGAATGCATC
CTGATCCAATTAAAGCGTCTGCCCAAGGAGACGGCAAAAGCGGAAAAAGTCATTTCCTCATACTTTGACCTGTTTGCCAA
AAAGGGATGGAAAGAGCTGTCAGAGAGAGCGGGGCTTTCGCTTTCAGAGCTTCAGGACATTCAAGACCTTGTCGCAGAAC
TGTCGCCGCGTCCCGGATTGATATATGGCACAGATGATGGCGAAGTCTATGTCGAGCCTGATTTAATCATTTCAGCAGAG
GATGGAAAATTGGTGCTTGAGCTCAGCAGCCGTTCTTTTCCCGACATCCAGCTTAACGCGTTTTACGAGCCGTTTTTAGA
GCGTCAGTCAAAAGACGAGGCGACCGCTTATATCTCTGATAAGTTCAAGCAGTGGAAATGGCTCGACAATGCCCTCAGGC
AAAGACGGGAAACGATGCGGAGGGTGATGGCGGAAATCATGGTGCGGCAAAAAGATTATTTTTTAAAAGGGAAAGACGAA
CTGAAGCCGCTCACATTAAAAGAGATCGCTGATGCCCTCGGCGTTCACGAATCAACGGTAAGCCGCGCAGTCAAAGGGAA
AATCGTGCAGACGCCTTTTGAGCTTTGCGAGCTTAAACGGTTTTTTTCGGCGAAGGTAAAGGACGCCGCTGACGGGCATG
CGTCAAGTCACACAGTCAAAGCGCATCTGCGCCGGCTGATCGATGAAGAAGATAAAATGAAACCGCTTTCAGATCAGAAA
CTGGCCGACCTTTTAAACGAAAAGCACGGCGTTGTCATATCGAGGCGGACCGTCGCCAAATACCGTGATCAGCTCAATAT
ACTGCCTTCGTCTGTAAGAAAGAGATATCAATAA

Upstream 100 bases:

>100_bases
GAAATAGGGCACGGAGAAAAGCTCTGTCAATGGTAACACTCCTTTTTTAAGTCTATACGGTTCGGAACCGATCGTGTAAA
CTAATAGTGGGAGGGGAGAT

Downstream 100 bases:

>100_bases
AGAAGCCGTCCGGCACGAGCGCCGGCGGCTTTTTTACGGGATCATCCACGAAAACAGATGATGCTGCAGGAAAGTGATCA
TGCAAACGAGGAAAAGAAGA

Product: SigL

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MMNLHVQQQQTAKLHLTQGLAQAIHMLQYSSAELNSRIDELSLENPLIERKDSDIPRSFYHKTNRHLQNEGISRSETAHS
REDVKSALKRQALDLQLSSREKRVFSYLVESLDPNGYVTEDIAFIASELAVTREEAEEVLGKLQTLDPAGIGARSLQECI
LIQLKRLPKETAKAEKVISSYFDLFAKKGWKELSERAGLSLSELQDIQDLVAELSPRPGLIYGTDDGEVYVEPDLIISAE
DGKLVLELSSRSFPDIQLNAFYEPFLERQSKDEATAYISDKFKQWKWLDNALRQRRETMRRVMAEIMVRQKDYFLKGKDE
LKPLTLKEIADALGVHESTVSRAVKGKIVQTPFELCELKRFFSAKVKDAADGHASSHTVKAHLRRLIDEEDKMKPLSDQK
LADLLNEKHGVVISRRTVAKYRDQLNILPSSVRKRYQ

Sequences:

>Translated_437_residues
MMNLHVQQQQTAKLHLTQGLAQAIHMLQYSSAELNSRIDELSLENPLIERKDSDIPRSFYHKTNRHLQNEGISRSETAHS
REDVKSALKRQALDLQLSSREKRVFSYLVESLDPNGYVTEDIAFIASELAVTREEAEEVLGKLQTLDPAGIGARSLQECI
LIQLKRLPKETAKAEKVISSYFDLFAKKGWKELSERAGLSLSELQDIQDLVAELSPRPGLIYGTDDGEVYVEPDLIISAE
DGKLVLELSSRSFPDIQLNAFYEPFLERQSKDEATAYISDKFKQWKWLDNALRQRRETMRRVMAEIMVRQKDYFLKGKDE
LKPLTLKEIADALGVHESTVSRAVKGKIVQTPFELCELKRFFSAKVKDAADGHASSHTVKAHLRRLIDEEDKMKPLSDQK
LADLLNEKHGVVISRRTVAKYRDQLNILPSSVRKRYQ
>Mature_437_residues
MMNLHVQQQQTAKLHLTQGLAQAIHMLQYSSAELNSRIDELSLENPLIERKDSDIPRSFYHKTNRHLQNEGISRSETAHS
REDVKSALKRQALDLQLSSREKRVFSYLVESLDPNGYVTEDIAFIASELAVTREEAEEVLGKLQTLDPAGIGARSLQECI
LIQLKRLPKETAKAEKVISSYFDLFAKKGWKELSERAGLSLSELQDIQDLVAELSPRPGLIYGTDDGEVYVEPDLIISAE
DGKLVLELSSRSFPDIQLNAFYEPFLERQSKDEATAYISDKFKQWKWLDNALRQRRETMRRVMAEIMVRQKDYFLKGKDE
LKPLTLKEIADALGVHESTVSRAVKGKIVQTPFELCELKRFFSAKVKDAADGHASSHTVKAHLRRLIDEEDKMKPLSDQK
LADLLNEKHGVVISRRTVAKYRDQLNILPSSVRKRYQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the levanase operon. The open complex (sigma-54 and core RNA poly

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=480, Percent_Identity=31.25, Blast_Score=221, Evalue=6e-59,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 49919; Mature: 49919

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNLHVQQQQTAKLHLTQGLAQAIHMLQYSSAELNSRIDELSLENPLIERKDSDIPRSFY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
HKTNRHLQNEGISRSETAHSREDVKSALKRQALDLQLSSREKRVFSYLVESLDPNGYVTE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
DIAFIASELAVTREEAEEVLGKLQTLDPAGIGARSLQECILIQLKRLPKETAKAEKVISS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
YFDLFAKKGWKELSERAGLSLSELQDIQDLVAELSPRPGLIYGTDDGEVYVEPDLIISAE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCEEEECC
DGKLVLELSSRSFPDIQLNAFYEPFLERQSKDEATAYISDKFKQWKWLDNALRQRRETMR
CCEEEEEECCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVMAEIMVRQKDYFLKGKDELKPLTLKEIADALGVHESTVSRAVKGKIVQTPFELCELKR
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCHHCCHHHHHHHHH
FFSAKVKDAADGHASSHTVKAHLRRLIDEEDKMKPLSDQKLADLLNEKHGVVISRRTVAK
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHCCCCCEEEEHHHHHH
YRDQLNILPSSVRKRYQ
HHHHHHCCCHHHHHCCC
>Mature Secondary Structure
MMNLHVQQQQTAKLHLTQGLAQAIHMLQYSSAELNSRIDELSLENPLIERKDSDIPRSFY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
HKTNRHLQNEGISRSETAHSREDVKSALKRQALDLQLSSREKRVFSYLVESLDPNGYVTE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
DIAFIASELAVTREEAEEVLGKLQTLDPAGIGARSLQECILIQLKRLPKETAKAEKVISS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
YFDLFAKKGWKELSERAGLSLSELQDIQDLVAELSPRPGLIYGTDDGEVYVEPDLIISAE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCEEEECC
DGKLVLELSSRSFPDIQLNAFYEPFLERQSKDEATAYISDKFKQWKWLDNALRQRRETMR
CCEEEEEECCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVMAEIMVRQKDYFLKGKDELKPLTLKEIADALGVHESTVSRAVKGKIVQTPFELCELKR
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCHHCCHHHHHHHHH
FFSAKVKDAADGHASSHTVKAHLRRLIDEEDKMKPLSDQKLADLLNEKHGVVISRRTVAK
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHCCCCCEEEEHHHHHH
YRDQLNILPSSVRKRYQ
HHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1924373; 8969506; 9384377 [H]