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The map label for this gene is ytsJ [H]

Identifier: 52786799

GI number: 52786799

Start: 2957590

End: 2958825

Strand: Reverse

Name: ytsJ [H]

Synonym: BLi03071

Alternate gene names: 52786799

Gene position: 2958825-2957590 (Counterclockwise)

Preceding gene: 52786800

Following gene: 52786798

Centisome position: 70.07

GC content: 50.24

Gene sequence:

>1236_bases
ATGTCATTGAAAGAAAAAGCACTGCATATGCACAAAGTAAACCAGGGCAAATTGGAATCAAAATCAAAAGTGCAGGTTCG
CAATGCGGACGATTTAAGCCTTGCATATTCTCCGGGTGTGGCTGAACCCTGCAAAGCGATTTACGATGATAACAGCAAAG
TATATGATTATACAATGAAGGGAAACATGGTAGCAGTCGTCTCGGACGGAACGGCTGTGCTCGGCCTCGGCAATATCGGC
CCTGAAGCCGCCCTCCCTGTGATGGAAGGAAAAGCGGTTCTTTTCAAAAGCTTTGCCGGTGTGGACGCTTTCCCAATCTG
TTTAAATACATCTGATGTCGATAAAATTGTTGAGACGGTCAAGCTGCTTGAGCCGACATTCGGCGGCGTCAACCTTGAAG
ACATTGCAGCTCCAAACTGTTTTGTCATTGAAGAACGCCTCAAAAAAGAAACGAAAATTCCGGTGTTTCACGATGACCAG
CACGGTACAGCAATCGTAACGGTAGCCGGTCTCGTCAATGCGCTTAAGCTGTCCGGCAAAACGATGTCTTCCGTCAAAGT
TGTGGCGAACGGCGCCGGCGCAGCGGGAATTGCCATCATTAAGCTTCTTTATCATTTCGGCGTGCGCGACATCATCATGT
GCGACACGAAAGGCGCGATCTACGAAGGCCGTCCGAACGGCATGAACGCAGTTAAAGAAGAAGTCGCAAAATATACGAAC
AAAAACCGCATAGACGGTTCGCTCAAAGACGTGATCAAAGACGCCGATGTCTTTATCGGGGTTTCCGTCGAAGGCGCTCT
GACAAAAGAAATGGTTGAAAGCATGGCTGACAAACCGATCATCTTCGCCATGGCGAATCCAAATCCTGAGATCATGCCTG
CCGAAGCGCATGAAGCCGGAGCAAGCGTCGTTGGAACGGGACGCTCTGACTTCCCTAACCAAGTGAACAACGTTCTCGCT
TTTCCTGGCATCTTCCGCGGGGCGCTCGACGTCCGCGCGACACATATCAACGAGGAGATGAAAATCGCCGCGGTTGAGGC
GATCGCGTCACTCGTTTCCGACGAAGAGCTGAGACCTGAATATGTCATTCCGGCTCCGTTCGATCCAAGGGTCGCACCAG
CTGTTGCCAAAGCGGTAGCCAAAGCCGCGATGGAAACAGGCGTTGCACGAATCGATGTCGATCCAGAAGAAGTCGCTGAA
AAAACAAGAAAGCTTGCAGTGATTGAAGGGAAATAA

Upstream 100 bases:

>100_bases
GTTATCATTTTTAAGATTCTGGGGTTCGTCTTACAAGAATCTTAAGCAGAAAACGAAGCAAAGTTTTTGGCAAAATTCCC
TTGAAGGAGTGTTTGTTAAC

Downstream 100 bases:

>100_bases
AAGTCCATTTTTGATCAGCGCGTGATTGCTCTGGCCTAAAGAGCCGTCACGCGCTGTTTATCGAATATGATGTTCGGTTT
TTCCTCTTATTGGGGACACT

Product: YtsJ

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MSLKEKALHMHKVNQGKLESKSKVQVRNADDLSLAYSPGVAEPCKAIYDDNSKVYDYTMKGNMVAVVSDGTAVLGLGNIG
PEAALPVMEGKAVLFKSFAGVDAFPICLNTSDVDKIVETVKLLEPTFGGVNLEDIAAPNCFVIEERLKKETKIPVFHDDQ
HGTAIVTVAGLVNALKLSGKTMSSVKVVANGAGAAGIAIIKLLYHFGVRDIIMCDTKGAIYEGRPNGMNAVKEEVAKYTN
KNRIDGSLKDVIKDADVFIGVSVEGALTKEMVESMADKPIIFAMANPNPEIMPAEAHEAGASVVGTGRSDFPNQVNNVLA
FPGIFRGALDVRATHINEEMKIAAVEAIASLVSDEELRPEYVIPAPFDPRVAPAVAKAVAKAAMETGVARIDVDPEEVAE
KTRKLAVIEGK

Sequences:

>Translated_411_residues
MSLKEKALHMHKVNQGKLESKSKVQVRNADDLSLAYSPGVAEPCKAIYDDNSKVYDYTMKGNMVAVVSDGTAVLGLGNIG
PEAALPVMEGKAVLFKSFAGVDAFPICLNTSDVDKIVETVKLLEPTFGGVNLEDIAAPNCFVIEERLKKETKIPVFHDDQ
HGTAIVTVAGLVNALKLSGKTMSSVKVVANGAGAAGIAIIKLLYHFGVRDIIMCDTKGAIYEGRPNGMNAVKEEVAKYTN
KNRIDGSLKDVIKDADVFIGVSVEGALTKEMVESMADKPIIFAMANPNPEIMPAEAHEAGASVVGTGRSDFPNQVNNVLA
FPGIFRGALDVRATHINEEMKIAAVEAIASLVSDEELRPEYVIPAPFDPRVAPAVAKAVAKAAMETGVARIDVDPEEVAE
KTRKLAVIEGK
>Mature_410_residues
SLKEKALHMHKVNQGKLESKSKVQVRNADDLSLAYSPGVAEPCKAIYDDNSKVYDYTMKGNMVAVVSDGTAVLGLGNIGP
EAALPVMEGKAVLFKSFAGVDAFPICLNTSDVDKIVETVKLLEPTFGGVNLEDIAAPNCFVIEERLKKETKIPVFHDDQH
GTAIVTVAGLVNALKLSGKTMSSVKVVANGAGAAGIAIIKLLYHFGVRDIIMCDTKGAIYEGRPNGMNAVKEEVAKYTNK
NRIDGSLKDVIKDADVFIGVSVEGALTKEMVESMADKPIIFAMANPNPEIMPAEAHEAGASVVGTGRSDFPNQVNNVLAF
PGIFRGALDVRATHINEEMKIAAVEAIASLVSDEELRPEYVIPAPFDPRVAPAVAKAVAKAAMETGVARIDVDPEEVAEK
TRKLAVIEGK

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=364, Percent_Identity=28.8461538461538, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI62420880, Length=364, Percent_Identity=28.8461538461538, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI239049447, Length=364, Percent_Identity=28.8461538461538, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI4505143, Length=394, Percent_Identity=25.1269035532995, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI4505145, Length=361, Percent_Identity=27.1468144044321, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI270265879, Length=327, Percent_Identity=27.217125382263, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788806, Length=406, Percent_Identity=52.9556650246305, Blast_Score=403, Evalue=1e-114,
Organism=Escherichia coli, GI87081919, Length=440, Percent_Identity=28.6363636363636, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17537199, Length=407, Percent_Identity=25.5528255528256, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322823, Length=390, Percent_Identity=27.6923076923077, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24646386, Length=409, Percent_Identity=27.3838630806846, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24646388, Length=409, Percent_Identity=28.1173594132029, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI78707242, Length=407, Percent_Identity=25.5528255528256, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI78707238, Length=407, Percent_Identity=25.5528255528256, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI281362674, Length=446, Percent_Identity=26.0089686098655, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281362672, Length=446, Percent_Identity=26.0089686098655, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356279, Length=446, Percent_Identity=26.0089686098655, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI78707240, Length=390, Percent_Identity=25.1282051282051, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI281363505, Length=411, Percent_Identity=23.6009732360097, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI78707236, Length=411, Percent_Identity=23.6009732360097, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281363503, Length=411, Percent_Identity=23.6009732360097, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI78707232, Length=364, Percent_Identity=25, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 43786; Mature: 43655

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKEKALHMHKVNQGKLESKSKVQVRNADDLSLAYSPGVAEPCKAIYDDNSKVYDYTMK
CCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEECCCCCHHHHHHHCCCCEEEEEEEC
GNMVAVVSDGTAVLGLGNIGPEAALPVMEGKAVLFKSFAGVDAFPICLNTSDVDKIVETV
CCEEEEEECCEEEEEECCCCCHHCCCHHCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHH
KLLEPTFGGVNLEDIAAPNCFVIEERLKKETKIPVFHDDQHGTAIVTVAGLVNALKLSGK
HHHCCCCCCCCHHHHCCCCEEEEHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHCCCC
TMSSVKVVANGAGAAGIAIIKLLYHFGVRDIIMCDTKGAIYEGRPNGMNAVKEEVAKYTN
CCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCHHHHHHHHHHHCC
KNRIDGSLKDVIKDADVFIGVSVEGALTKEMVESMADKPIIFAMANPNPEIMPAEAHEAG
CCCCCCHHHHHHCCCCEEEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCEEECCHHHCC
ASVVGTGRSDFPNQVNNVLAFPGIFRGALDVRATHINEEMKIAAVEAIASLVSDEELRPE
CCEEECCCCCCCHHHHHEEECCHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCCC
YVIPAPFDPRVAPAVAKAVAKAAMETGVARIDVDPEEVAEKTRKLAVIEGK
EEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHEEEEEECC
>Mature Secondary Structure 
SLKEKALHMHKVNQGKLESKSKVQVRNADDLSLAYSPGVAEPCKAIYDDNSKVYDYTMK
CHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEECCCCCHHHHHHHCCCCEEEEEEEC
GNMVAVVSDGTAVLGLGNIGPEAALPVMEGKAVLFKSFAGVDAFPICLNTSDVDKIVETV
CCEEEEEECCEEEEEECCCCCHHCCCHHCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHH
KLLEPTFGGVNLEDIAAPNCFVIEERLKKETKIPVFHDDQHGTAIVTVAGLVNALKLSGK
HHHCCCCCCCCHHHHCCCCEEEEHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHCCCC
TMSSVKVVANGAGAAGIAIIKLLYHFGVRDIIMCDTKGAIYEGRPNGMNAVKEEVAKYTN
CCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCHHHHHHHHHHHCC
KNRIDGSLKDVIKDADVFIGVSVEGALTKEMVESMADKPIIFAMANPNPEIMPAEAHEAG
CCCCCCHHHHHHCCCCEEEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCEEECCHHHCC
ASVVGTGRSDFPNQVNNVLAFPGIFRGALDVRATHINEEMKIAAVEAIASLVSDEELRPE
CCEEECCCCCCCHHHHHEEECCHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCCC
YVIPAPFDPRVAPAVAKAVAKAAMETGVARIDVDPEEVAEKTRKLAVIEGK
EEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]