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The map label for this gene is araM

Identifier: 52786755

GI number: 52786755

Start: 2910235

End: 2911440

Strand: Reverse

Name: araM

Synonym: BLi03025

Alternate gene names: 52786755

Gene position: 2911440-2910235 (Counterclockwise)

Preceding gene: 52786756

Following gene: 52786754

Centisome position: 68.95

GC content: 52.82

Gene sequence:

>1206_bases
ATGAATGACTTGATCTCTTATGTGAAAAAAACTTTGGGCGCATGCGAATGCGGAACCGTCCACCACCCGCTTACAGTCGA
AAAAATCGCGATCGGCGACAATGCTGTTGAGCAAGAGCTGCCCGCTTTCGTGAAATCGGCTTCCTATAAAAAAGCCGCTG
TCATTTATGATGAAACAACCGGACGGTTAGCCGGCAAGCGGATTGCCTCTCTTCTTGAGGAAACGGCTGAAACGGTTCCG
GTGCTGCTGGAAGCTAATGAAGCGGGCGATGTGACGGCGGATGAGCAAACATTGGTGAGTGCATTGATCGGTGTACCGAT
TGATGCGGATGTACTAATTGCGGCCGGAGCGGGCACGATTCACGACATCGTCAGGTTTTGCGCCTATCAGCGCGGCATTC
CGTTTATTTCAGTTCCTACGGCTCCGTCGGTTGACGGTTTTACTTCAGCCGGCGCACCGCTGATTTTAAAAGGAAAGAAA
CAGACGGTTCAAACGACAGCGCCGATCGCGCTTTTTGCCGATTTGGAGCTCTTGTGTCAAGCGCCGCAGAACATGGTAGC
CGCAGGTTTTGGCGATATGCTCGGCAAGGTGACGTCGCTTGCGGATTGGGAGATTTCCCGCTTGCTTGCCGGCGAGCCGT
ATTGTCCGGCCGCCAGCCGGCTCACGAGGGAAGCGCTTGATCAATGTCTTGACCGAAAAGATGACATCGCCGCCAAGATG
CGGGATGGGATTGAGAAATTGATGGAATCGCTGATCCTTTCTGGCCTAGTCATGCTGGTTCTCGACCATTCAAGGCCGGC
TTCCGGCGGCGAGCACCATCTTTCCCACTACCTTGAAATGAAAGCGCTGGAGAACAATAAGCGGCAAGTGCTCCACGGTG
CTAAAGTCGGCTGCAGCGCGATTATGCTGACTGACATTTACCGATCTCTTATCGGTGCAAGCCTGGGTGATCAACACGCT
GAGCAAGCGATTCGCTCCGTTTATGAAAAGCTCCCTGACGGTAAGAAAATGGCAGAGTGGATGAGGCGTATCGGCGGGCC
TGTATCATTCAAAGAACTCGATGTTGAAGAAGAGCTGGTGAGAGAAGCGCTCGCATACGCCCATCAGCTCAGAGACCGGT
ATACGGGACTGAAAATCATCAATCAATACGGCCTTTTGCCGGGGCTTTTAGGCAAAGGACCAGGCGTGAAAGGGGTTAAA
ATGTGA

Upstream 100 bases:

>100_bases
CCGTCTCTTAAGCGGATTAAGAGCGCTTTGCAGGATAAGCATTATTTTCGAAAGCACGGGGCAGACGCCTATTACGGCCA
ATAGAGGAGGGAAGCTGCCA

Downstream 100 bases:

>100_bases
AAAGGTTCCTTTCATCTATCTTTATGGTCACGGTCGCTGTATGTTTGCTTTTATCGGGGTGCAAGGCGAGTCCTGCCTCC
GATCAAGCGGACGGCACCGA

Product: AraM

Products: NA

Alternate protein names: G1P dehydrogenase; G1PDH; Enantiomeric glycerophosphate synthase; sn-glycerol-1-phosphate dehydrogenase

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNDLISYVKKTLGACECGTVHHPLTVEKIAIGDNAVEQELPAFVKSASYKKAAVIYDETTGRLAGKRIASLLEETAETVP
VLLEANEAGDVTADEQTLVSALIGVPIDADVLIAAGAGTIHDIVRFCAYQRGIPFISVPTAPSVDGFTSAGAPLILKGKK
QTVQTTAPIALFADLELLCQAPQNMVAAGFGDMLGKVTSLADWEISRLLAGEPYCPAASRLTREALDQCLDRKDDIAAKM
RDGIEKLMESLILSGLVMLVLDHSRPASGGEHHLSHYLEMKALENNKRQVLHGAKVGCSAIMLTDIYRSLIGASLGDQHA
EQAIRSVYEKLPDGKKMAEWMRRIGGPVSFKELDVEEELVREALAYAHQLRDRYTGLKIINQYGLLPGLLGKGPGVKGVK
M

Sequences:

>Translated_401_residues
MNDLISYVKKTLGACECGTVHHPLTVEKIAIGDNAVEQELPAFVKSASYKKAAVIYDETTGRLAGKRIASLLEETAETVP
VLLEANEAGDVTADEQTLVSALIGVPIDADVLIAAGAGTIHDIVRFCAYQRGIPFISVPTAPSVDGFTSAGAPLILKGKK
QTVQTTAPIALFADLELLCQAPQNMVAAGFGDMLGKVTSLADWEISRLLAGEPYCPAASRLTREALDQCLDRKDDIAAKM
RDGIEKLMESLILSGLVMLVLDHSRPASGGEHHLSHYLEMKALENNKRQVLHGAKVGCSAIMLTDIYRSLIGASLGDQHA
EQAIRSVYEKLPDGKKMAEWMRRIGGPVSFKELDVEEELVREALAYAHQLRDRYTGLKIINQYGLLPGLLGKGPGVKGVK
M
>Mature_401_residues
MNDLISYVKKTLGACECGTVHHPLTVEKIAIGDNAVEQELPAFVKSASYKKAAVIYDETTGRLAGKRIASLLEETAETVP
VLLEANEAGDVTADEQTLVSALIGVPIDADVLIAAGAGTIHDIVRFCAYQRGIPFISVPTAPSVDGFTSAGAPLILKGKK
QTVQTTAPIALFADLELLCQAPQNMVAAGFGDMLGKVTSLADWEISRLLAGEPYCPAASRLTREALDQCLDRKDDIAAKM
RDGIEKLMESLILSGLVMLVLDHSRPASGGEHHLSHYLEMKALENNKRQVLHGAKVGCSAIMLTDIYRSLIGASLGDQHA
EQAIRSVYEKLPDGKKMAEWMRRIGGPVSFKELDVEEELVREALAYAHQLRDRYTGLKIINQYGLLPGLLGKGPGVKGVK
M

Specific function: Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is probably used for the synthesis of phosphoglycerolipids in Gram-positive bacterial species

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycerol-1-phosphate dehydrogenase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): G1PDH_BACLD (Q65GC3)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_080170.1
- RefSeq:   YP_092584.1
- ProteinModelPortal:   Q65GC3
- SMR:   Q65GC3
- STRING:   Q65GC3
- EnsemblBacteria:   EBBACT00000056067
- EnsemblBacteria:   EBBACT00000059890
- GeneID:   3028655
- GeneID:   3098580
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03025
- KEGG:   bli:BL00349
- NMPDR:   fig|279010.5.peg.3373
- eggNOG:   COG0371
- GeneTree:   EBGT00050000001321
- HOGENOM:   HBG313183
- OMA:   NIRERFT
- ProtClustDB:   CLSK873152
- BioCyc:   BLIC279010-1:BLI03025-MONOMER
- BioCyc:   BLIC279010:BL00349-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00497_B
- InterPro:   IPR002658
- InterPro:   IPR023003

Pfam domain/function: PF01761 DHQ_synthase

EC number: =1.1.1.261

Molecular weight: Translated: 43198; Mature: 43198

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: BINDING 57-57 BINDING 145-145 BINDING 149-149 BINDING 192-192 BINDING 276-276

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLISYVKKTLGACECGTVHHPLTVEKIAIGDNAVEQELPAFVKSASYKKAAVIYDETT
CHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCC
GRLAGKRIASLLEETAETVPVLLEANEAGDVTADEQTLVSALIGVPIDADVLIAAGAGTI
CHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHH
HDIVRFCAYQRGIPFISVPTAPSVDGFTSAGAPLILKGKKQTVQTTAPIALFADLELLCQ
HHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHCCHHHHHHHHHHHH
APQNMVAAGFGDMLGKVTSLADWEISRLLAGEPYCPAASRLTREALDQCLDRKDDIAAKM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
RDGIEKLMESLILSGLVMLVLDHSRPASGGEHHLSHYLEMKALENNKRQVLHGAKVGCSA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
IMLTDIYRSLIGASLGDQHAEQAIRSVYEKLPDGKKMAEWMRRIGGPVSFKELDVEEELV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHCCHHHHHH
REALAYAHQLRDRYTGLKIINQYGLLPGLLGKGPGVKGVKM
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNDLISYVKKTLGACECGTVHHPLTVEKIAIGDNAVEQELPAFVKSASYKKAAVIYDETT
CHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCC
GRLAGKRIASLLEETAETVPVLLEANEAGDVTADEQTLVSALIGVPIDADVLIAAGAGTI
CHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHH
HDIVRFCAYQRGIPFISVPTAPSVDGFTSAGAPLILKGKKQTVQTTAPIALFADLELLCQ
HHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHCCHHHHHHHHHHHH
APQNMVAAGFGDMLGKVTSLADWEISRLLAGEPYCPAASRLTREALDQCLDRKDDIAAKM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
RDGIEKLMESLILSGLVMLVLDHSRPASGGEHHLSHYLEMKALENNKRQVLHGAKVGCSA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
IMLTDIYRSLIGASLGDQHAEQAIRSVYEKLPDGKKMAEWMRRIGGPVSFKELDVEEELV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHCCHHHHHH
REALAYAHQLRDRYTGLKIINQYGLLPGLLGKGPGVKGVKM
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA