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The map label for this gene is pheS

Identifier: 52786746

GI number: 52786746

Start: 2900699

End: 2901733

Strand: Reverse

Name: pheS

Synonym: BLi03016

Alternate gene names: 52786746

Gene position: 2901733-2900699 (Counterclockwise)

Preceding gene: 52786747

Following gene: 52786745

Centisome position: 68.72

GC content: 49.37

Gene sequence:

>1035_bases
ATGCAGGAAGAGCTGAAACGGCTTGAAAAAGAAGCGGTTGAAAAAGTGGAAGCCGCTGGTTCATTAAAAGAAGTCAACGA
TGTCCGCGTTGCGTATCTGGGAAAAAAGGGACCGATCACAGAAGTGCTTCGGGGAATGGGAAAGCTGTCCGCTGAAGAAA
GGCCGAAAATGGGGGCCTTGGCGAACGAAGTAAGAGAGAAAATCGCCGCGGCGATCGCCGAAAAAAATGCCCGCCTTGAG
GAAGAAGAGGTGAAAAGGAAGCTGAAAGAGCAGACGATTGACGTGACGCTTCCGGGAAGCCCTGTGAAAACAGGAGCGCG
GCATCCGCTGACGATCGTTATTGAAGAAATCGAAGACTTGTTTATCAGCATGGGATATTCGGTGGAAGAGGGGCCTGAAG
TGGAAACCGACTATTATAACTTCGAAGCGCTCAACCTTCCGAAAGAGCACCCGGCCCGGGATATGCAAGACAGCTTTTAT
ATTACGGAAGATACGCTGATGAGAACACAGACATCTCCTGTTCAGACGCGCACGATGGAAAAACATAAAGGCAAAGGCCC
TGTCAAAATCATCTGCCCCGGCAAAGTGTACCGCCGCGACAACGATGATGCGACACATTCCCATCAGTTTATGCAGATTG
AAGGGCTCTGCGTTGACCGTGATATCAGCATGAGCGACTTAAAAGGAACCCTGGAAACGGTCGCGAAAAAGATGTTCGGC
GAAGAGCGCGAAATCAGACTGCGTCCAAGCTTCTTCCCGTTTACAGAGCCGTCTGTCGAAGTCGATGTTTCCTGCTTTAA
ATGCGGAGGCAAAGGCTGCTCCGTTTGTAAACAAACGGGCTGGATCGAAATTCTCGGCGCCGGAATGGTGCATCCGAACG
TGCTCGAAATGGCCGGATTCGATTCCAAACAATATCAGGGATTTGCTTTTGGGATGGGCGTTGAACGGATCGCAATGCTG
AAGTACGGCATTGACGACATTCGTCATTTCTATACGAACGATGTCAGATTTTTATCTCAATTTAAACAAGCTTAA

Upstream 100 bases:

>100_bases
AAGGGTGGTACCACGGCCACGATTCGTCCCTTTCATTATAAAAGGGGCTTTTTTTATTGCCTAAAAAACAGGTTGCTACA
TAAAAGGAGGAGTTTTTAAG

Downstream 100 bases:

>100_bases
AGAGGAGGACGCGTGATGTTTGTTTCATATAAATGGCTGCAAGAATACGTGAATTTAGACGGGATGACTCCCGAGATTCT
CGCTGAAAAGATTACAAGAA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MQEELKRLEKEAVEKVEAAGSLKEVNDVRVAYLGKKGPITEVLRGMGKLSAEERPKMGALANEVREKIAAAIAEKNARLE
EEEVKRKLKEQTIDVTLPGSPVKTGARHPLTIVIEEIEDLFISMGYSVEEGPEVETDYYNFEALNLPKEHPARDMQDSFY
ITEDTLMRTQTSPVQTRTMEKHKGKGPVKIICPGKVYRRDNDDATHSHQFMQIEGLCVDRDISMSDLKGTLETVAKKMFG
EEREIRLRPSFFPFTEPSVEVDVSCFKCGGKGCSVCKQTGWIEILGAGMVHPNVLEMAGFDSKQYQGFAFGMGVERIAML
KYGIDDIRHFYTNDVRFLSQFKQA

Sequences:

>Translated_344_residues
MQEELKRLEKEAVEKVEAAGSLKEVNDVRVAYLGKKGPITEVLRGMGKLSAEERPKMGALANEVREKIAAAIAEKNARLE
EEEVKRKLKEQTIDVTLPGSPVKTGARHPLTIVIEEIEDLFISMGYSVEEGPEVETDYYNFEALNLPKEHPARDMQDSFY
ITEDTLMRTQTSPVQTRTMEKHKGKGPVKIICPGKVYRRDNDDATHSHQFMQIEGLCVDRDISMSDLKGTLETVAKKMFG
EEREIRLRPSFFPFTEPSVEVDVSCFKCGGKGCSVCKQTGWIEILGAGMVHPNVLEMAGFDSKQYQGFAFGMGVERIAML
KYGIDDIRHFYTNDVRFLSQFKQA
>Mature_344_residues
MQEELKRLEKEAVEKVEAAGSLKEVNDVRVAYLGKKGPITEVLRGMGKLSAEERPKMGALANEVREKIAAAIAEKNARLE
EEEVKRKLKEQTIDVTLPGSPVKTGARHPLTIVIEEIEDLFISMGYSVEEGPEVETDYYNFEALNLPKEHPARDMQDSFY
ITEDTLMRTQTSPVQTRTMEKHKGKGPVKIICPGKVYRRDNDDATHSHQFMQIEGLCVDRDISMSDLKGTLETVAKKMFG
EEREIRLRPSFFPFTEPSVEVDVSCFKCGGKGCSVCKQTGWIEILGAGMVHPNVLEMAGFDSKQYQGFAFGMGVERIAML
KYGIDDIRHFYTNDVRFLSQFKQA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=355, Percent_Identity=26.7605633802817, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI4758340, Length=279, Percent_Identity=27.5985663082437, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI1788007, Length=339, Percent_Identity=46.9026548672566, Blast_Score=327, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI32566635, Length=254, Percent_Identity=33.4645669291339, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32563657, Length=274, Percent_Identity=28.8321167883212, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17508957, Length=274, Percent_Identity=28.8321167883212, Blast_Score=124, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6321087, Length=348, Percent_Identity=26.4367816091954, Blast_Score=132, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6325304, Length=346, Percent_Identity=26.878612716763, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI18858079, Length=286, Percent_Identity=28.6713286713287, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI17137424, Length=294, Percent_Identity=27.2108843537415, Blast_Score=107, Evalue=8e-24,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_BACLD (Q65GD2)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_080162.1
- RefSeq:   YP_092575.1
- ProteinModelPortal:   Q65GD2
- SMR:   Q65GD2
- STRING:   Q65GD2
- EnsemblBacteria:   EBBACT00000054761
- EnsemblBacteria:   EBBACT00000059050
- GeneID:   3028881
- GeneID:   3098552
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03016
- KEGG:   bli:BL00340
- NMPDR:   fig|279010.5.peg.3364
- eggNOG:   COG0016
- GeneTree:   EBGT00050000002461
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   BLIC279010-1:BLI03016-MONOMER
- BioCyc:   BLIC279010:BL00340-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38799; Mature: 38799

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEELKRLEKEAVEKVEAAGSLKEVNDVRVAYLGKKGPITEVLRGMGKLSAEERPKMGAL
CHHHHHHHHHHHHHHHHHCCCHHHHCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHH
ANEVREKIAAAIAEKNARLEEEEVKRKLKEQTIDVTLPGSPVKTGARHPLTIVIEEIEDL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
FISMGYSVEEGPEVETDYYNFEALNLPKEHPARDMQDSFYITEDTLMRTQTSPVQTRTME
HHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEHHHHHHCCCCCHHHHHHH
KHKGKGPVKIICPGKVYRRDNDDATHSHQFMQIEGLCVDRDISMSDLKGTLETVAKKMFG
HHCCCCCEEEEECCHHEECCCCCCCCCCCEEEEECEEECCCCCHHHHHHHHHHHHHHHCC
EEREIRLRPSFFPFTEPSVEVDVSCFKCGGKGCSVCKQTGWIEILGAGMVHPNVLEMAGF
CCCEEEECCCCCCCCCCCCEEEEEEEECCCCCHHHHHHCCCEEEEECCCCCCCHHHHCCC
DSKQYQGFAFGMGVERIAMLKYGIDDIRHFYTNDVRFLSQFKQA
CCCCCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQEELKRLEKEAVEKVEAAGSLKEVNDVRVAYLGKKGPITEVLRGMGKLSAEERPKMGAL
CHHHHHHHHHHHHHHHHHCCCHHHHCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHH
ANEVREKIAAAIAEKNARLEEEEVKRKLKEQTIDVTLPGSPVKTGARHPLTIVIEEIEDL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
FISMGYSVEEGPEVETDYYNFEALNLPKEHPARDMQDSFYITEDTLMRTQTSPVQTRTME
HHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEHHHHHHCCCCCHHHHHHH
KHKGKGPVKIICPGKVYRRDNDDATHSHQFMQIEGLCVDRDISMSDLKGTLETVAKKMFG
HHCCCCCEEEEECCHHEECCCCCCCCCCCEEEEECEEECCCCCHHHHHHHHHHHHHHHCC
EEREIRLRPSFFPFTEPSVEVDVSCFKCGGKGCSVCKQTGWIEILGAGMVHPNVLEMAGF
CCCEEEECCCCCCCCCCCCEEEEEEEECCCCCHHHHHHCCCEEEEECCCCCCCHHHHCCC
DSKQYQGFAFGMGVERIAMLKYGIDDIRHFYTNDVRFLSQFKQA
CCCCCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA