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The map label for this gene is pheT

Identifier: 52786745

GI number: 52786745

Start: 2898269

End: 2900683

Strand: Reverse

Name: pheT

Synonym: BLi03015

Alternate gene names: 52786745

Gene position: 2900683-2898269 (Counterclockwise)

Preceding gene: 52786746

Following gene: 52786739

Centisome position: 68.69

GC content: 51.1

Gene sequence:

>2415_bases
ATGTTTGTTTCATATAAATGGCTGCAAGAATACGTGAATTTAGACGGGATGACTCCCGAGATTCTCGCTGAAAAGATTAC
AAGAAGCGGAATTGAAGTGGAAGGCATCGAATATAAGGGAGAAGGAATTAAAGGTGTTGTCATTGGACATGTGCTGGAGC
GCGAACAGCATCCGAACGCCGACAAATTAAACAAATGCCTCGTCGATATCGGCGAAGAAGAGCCTGTCCAGATCATCTGC
GGGGCTCCGAATGTGGACAAAGGGCAAAAAGTTGCCGTCGCCACGGTTGGCGCGGTATTGCCCGGCAATTTTAAAATCAA
AAAGGCGAAGCTCCGCGGGGAAGCGTCTAACGGCATGATATGCTCTTTGCAGGAGCTTGGCATTCAAGGCAAGCTTGTGC
CGAAAGAATATGCGGAAGGCATCTTCGTTTTCCCGGGCGATGCTGAAACCGGGGCGGACGCCCTTCAAAGCCTGGCGCTC
GACGATGCGGTTCTCGAACTGGGACTGACGCCAAACCGCGCCGACGCCATGAACATGCTCGGCGTTGCCTATGAAGTGGC
TGCGATTCTCGGCCGCGAAGTCAAGCTCCCTGAGACGGCTTATCAGGCAGGCGCCGAAAAAGCGGCGGACTATATTTCCG
TCAAAATTGAAGATCCTGAAGCCAATCCTTTATACGCGGCGAAAATCATTAAAGACGTCAAAATCGGCCCGTCGCCGCTT
TGGATGCAGACGAAGCTGATCAACGCCGGCATCAGACCGCACAACAATGTCGTCGACGTTACGAACTTTGTGCTGCTCGA
ATACGGCCAGCCGCTCCACGCGTTTGATTATGACCGCTTCGGCTCAAAAGAGGTCGTCATCCGCAAAGCGTACGAGAACG
AAACGATAACGACGCTCGACGAACAAGAAAGAACGCTGACCACTGAGCACCTTGTCATCACAAACGGCTCTTCTCCGCAG
GCTGTCGCAGGCGTAATGGGCGGAGCGGAATCAGAAGTCCGCGAAGATACTGCGACAGTGCTGCTGGAAGCGGCGTATTT
CAACGGCCAGACCGTTCGCAAAGCATCAAAGGACCTCGGTCTTCGCAGCGAATCAAGCGTCCGCTTTGAAAAAGGCATCG
ATCCTGCACGGGTGCGCCTCGCTGCAGAACGCGCGGCAGATTTGATCAGCCGGTATGCCGGAGGAACCGTCTTGGAAGGT
ACGGTCGAAGAAAACCATCTTGACATCAAAGAGAATGTCATCCGCCTTTCCGTTGATAAAGTGACGAAGGTTCTCGGCAT
GTCCATCAGCAAAGAAGAAATGGTCAACATTTTTGAAAGGCTCGGCTTTGCAGTTGAAGACGCTGAACGTGAACTGGTCG
TGACGGTTCCGTCAAGAAGAGGCGATATTGCGATTGAAGAAGATTTGATCGAAGAGGTCGCAAGACTGTACGGATATGAC
AACATTCCGTCGACACTGCCGGAAGTAACAGGCTTTGCGGGCGGACTGACACCTTATCAGGACAAGCGCAGAAAAGTAAG
ACGCTTCCTGGAAGGAGCAGGGCTTTCCCAGGCGATCACATACTCATTAACAAACGATAAAAAAGCGACGGCTTACGCAT
TGGAAAAATCGTTTAAAACGATCCTTTCACTGCCGATGAGCGAGGAAAGAAGCGTGCTGAGGCACAGCCTGCTCCCTAAT
CTGCTCGATTCGGTTGCATACAACTTGGCAAGGCAGGCCGATTCAGCTGCTTTTTATGAAATCGGTTCTGTTTTCTTGAA
AGCCGAAGAACATACGAAACCGGTCGAAAAAGAACACGTCGCCGGAGCCGTGACAGGCCTGTGGCACAAAAACCTCTGGC
AGGGCGAGAAAAAGCCGGTTGATTTCTTCGTCGTAAAAGGCATCGTCGAAGGTCTGTTGGAAAAGCTCGGAATCACCGAA
GGCATCGAATTCGCTCAATCTGAAAGAAAAGAGCTTCATCCTGGGCGGACAGCCAATATTTTGCACAACGGCTCATTAGT
CGGCTTCATCGGCCAGCTGCATCCGTCAGTTGAAAAAGAGCTGGACCTGAGCGACACGTATGTGTTTGAACTGGACCTGC
ACGAGCTCCTCCGATTGGATGTACCGGAGATCACGTATACACCAATTCCGAAGTATCCTTCTGTTACAAGGGACATCGCG
CTTGTTGTAGACAAACAAACAACGGCCGGCCAGCTTGAGGACGTCATCAAAACAGCAGGCGGCAAATGGCTGAAAGAAGT
CCATGTCTTTGACGTCTATGAAGGCGAGCACATGGAAGAAGGCAAGAAGTCCGTCGCCTTCAGCCTGCAATATTTGAATC
CTGAACAGACATTGACAGAAGAAGAAGTGACAAAAGTCCACGACCAAGTGCTGAAAGCGCTGGAAGATAAATATCAGGCT
GTATTGAGAGGATAA

Upstream 100 bases:

>100_bases
CGCAATGCTGAAGTACGGCATTGACGACATTCGTCATTTCTATACGAACGATGTCAGATTTTTATCTCAATTTAAACAAG
CTTAAAGAGGAGGACGCGTG

Downstream 100 bases:

>100_bases
AGAAAAACCGGTCCCCGATCATTTGGGAACCGGTTTTTTTGCATTGGAAAAATTGTCGGCCTATTGTAAGTTTTAATTAT
TTTAGGATGGCAAGAATATT

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 804; Mature: 804

Protein sequence:

>804_residues
MFVSYKWLQEYVNLDGMTPEILAEKITRSGIEVEGIEYKGEGIKGVVIGHVLEREQHPNADKLNKCLVDIGEEEPVQIIC
GAPNVDKGQKVAVATVGAVLPGNFKIKKAKLRGEASNGMICSLQELGIQGKLVPKEYAEGIFVFPGDAETGADALQSLAL
DDAVLELGLTPNRADAMNMLGVAYEVAAILGREVKLPETAYQAGAEKAADYISVKIEDPEANPLYAAKIIKDVKIGPSPL
WMQTKLINAGIRPHNNVVDVTNFVLLEYGQPLHAFDYDRFGSKEVVIRKAYENETITTLDEQERTLTTEHLVITNGSSPQ
AVAGVMGGAESEVREDTATVLLEAAYFNGQTVRKASKDLGLRSESSVRFEKGIDPARVRLAAERAADLISRYAGGTVLEG
TVEENHLDIKENVIRLSVDKVTKVLGMSISKEEMVNIFERLGFAVEDAERELVVTVPSRRGDIAIEEDLIEEVARLYGYD
NIPSTLPEVTGFAGGLTPYQDKRRKVRRFLEGAGLSQAITYSLTNDKKATAYALEKSFKTILSLPMSEERSVLRHSLLPN
LLDSVAYNLARQADSAAFYEIGSVFLKAEEHTKPVEKEHVAGAVTGLWHKNLWQGEKKPVDFFVVKGIVEGLLEKLGITE
GIEFAQSERKELHPGRTANILHNGSLVGFIGQLHPSVEKELDLSDTYVFELDLHELLRLDVPEITYTPIPKYPSVTRDIA
LVVDKQTTAGQLEDVIKTAGGKWLKEVHVFDVYEGEHMEEGKKSVAFSLQYLNPEQTLTEEEVTKVHDQVLKALEDKYQA
VLRG

Sequences:

>Translated_804_residues
MFVSYKWLQEYVNLDGMTPEILAEKITRSGIEVEGIEYKGEGIKGVVIGHVLEREQHPNADKLNKCLVDIGEEEPVQIIC
GAPNVDKGQKVAVATVGAVLPGNFKIKKAKLRGEASNGMICSLQELGIQGKLVPKEYAEGIFVFPGDAETGADALQSLAL
DDAVLELGLTPNRADAMNMLGVAYEVAAILGREVKLPETAYQAGAEKAADYISVKIEDPEANPLYAAKIIKDVKIGPSPL
WMQTKLINAGIRPHNNVVDVTNFVLLEYGQPLHAFDYDRFGSKEVVIRKAYENETITTLDEQERTLTTEHLVITNGSSPQ
AVAGVMGGAESEVREDTATVLLEAAYFNGQTVRKASKDLGLRSESSVRFEKGIDPARVRLAAERAADLISRYAGGTVLEG
TVEENHLDIKENVIRLSVDKVTKVLGMSISKEEMVNIFERLGFAVEDAERELVVTVPSRRGDIAIEEDLIEEVARLYGYD
NIPSTLPEVTGFAGGLTPYQDKRRKVRRFLEGAGLSQAITYSLTNDKKATAYALEKSFKTILSLPMSEERSVLRHSLLPN
LLDSVAYNLARQADSAAFYEIGSVFLKAEEHTKPVEKEHVAGAVTGLWHKNLWQGEKKPVDFFVVKGIVEGLLEKLGITE
GIEFAQSERKELHPGRTANILHNGSLVGFIGQLHPSVEKELDLSDTYVFELDLHELLRLDVPEITYTPIPKYPSVTRDIA
LVVDKQTTAGQLEDVIKTAGGKWLKEVHVFDVYEGEHMEEGKKSVAFSLQYLNPEQTLTEEEVTKVHDQVLKALEDKYQA
VLRG
>Mature_804_residues
MFVSYKWLQEYVNLDGMTPEILAEKITRSGIEVEGIEYKGEGIKGVVIGHVLEREQHPNADKLNKCLVDIGEEEPVQIIC
GAPNVDKGQKVAVATVGAVLPGNFKIKKAKLRGEASNGMICSLQELGIQGKLVPKEYAEGIFVFPGDAETGADALQSLAL
DDAVLELGLTPNRADAMNMLGVAYEVAAILGREVKLPETAYQAGAEKAADYISVKIEDPEANPLYAAKIIKDVKIGPSPL
WMQTKLINAGIRPHNNVVDVTNFVLLEYGQPLHAFDYDRFGSKEVVIRKAYENETITTLDEQERTLTTEHLVITNGSSPQ
AVAGVMGGAESEVREDTATVLLEAAYFNGQTVRKASKDLGLRSESSVRFEKGIDPARVRLAAERAADLISRYAGGTVLEG
TVEENHLDIKENVIRLSVDKVTKVLGMSISKEEMVNIFERLGFAVEDAERELVVTVPSRRGDIAIEEDLIEEVARLYGYD
NIPSTLPEVTGFAGGLTPYQDKRRKVRRFLEGAGLSQAITYSLTNDKKATAYALEKSFKTILSLPMSEERSVLRHSLLPN
LLDSVAYNLARQADSAAFYEIGSVFLKAEEHTKPVEKEHVAGAVTGLWHKNLWQGEKKPVDFFVVKGIVEGLLEKLGITE
GIEFAQSERKELHPGRTANILHNGSLVGFIGQLHPSVEKELDLSDTYVFELDLHELLRLDVPEITYTPIPKYPSVTRDIA
LVVDKQTTAGQLEDVIKTAGGKWLKEVHVFDVYEGEHMEEGKKSVAFSLQYLNPEQTLTEEEVTKVHDQVLKALEDKYQA
VLRG

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI124028525, Length=278, Percent_Identity=25.1798561151079, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1788006, Length=809, Percent_Identity=38.6897404202719, Blast_Score=496, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17533403, Length=577, Percent_Identity=21.1438474870017, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21355211, Length=289, Percent_Identity=25.9515570934256, Blast_Score=97, Evalue=6e-20,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_BACLD (Q65GD3)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080161.1
- RefSeq:   YP_092574.1
- ProteinModelPortal:   Q65GD3
- SMR:   Q65GD3
- STRING:   Q65GD3
- EnsemblBacteria:   EBBACT00000056365
- EnsemblBacteria:   EBBACT00000060396
- GeneID:   3029084
- GeneID:   3098551
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03015
- KEGG:   bli:BL00339
- NMPDR:   fig|279010.5.peg.3363
- eggNOG:   COG0072
- GeneTree:   EBGT00050000001777
- HOGENOM:   HBG399638
- OMA:   ADKLRVC
- ProtClustDB:   PRK00629
- BioCyc:   BLIC279010-1:BLI03015-MONOMER
- BioCyc:   BLIC279010:BL00339-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00283
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547
- Gene3D:   G3DSA:3.50.40.10
- Gene3D:   G3DSA:3.30.56.20
- Gene3D:   G3DSA:3.30.70.380
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00873
- SMART:   SM00874
- SMART:   SM00896
- TIGRFAMs:   TIGR00472

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind; SSF56037 B3_4; SSF54991 Fdx_AntiC_bd; SSF50249 Nucleic_acid_OB; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 88636; Mature: 88636

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS51483 B5; PS51447 FDX_ACB; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVSYKWLQEYVNLDGMTPEILAEKITRSGIEVEGIEYKGEGIKGVVIGHVLEREQHPNA
CCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCEEHHHHHHHHCCCCCH
DKLNKCLVDIGEEEPVQIICGAPNVDKGQKVAVATVGAVLPGNFKIKKAKLRGEASNGMI
HHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEEECEECCCCEEEEEEEECCCCCCCEE
CSLQELGIQGKLVPKEYAEGIFVFPGDAETGADALQSLALDDAVLELGLTPNRADAMNML
EEHHHCCCCCCCCCHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
GVAYEVAAILGREVKLPETAYQAGAEKAADYISVKIEDPEANPLYAAKIIKDVKIGPSPL
HHHHHHHHHHCCCCCCCHHHHHCCHHHCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCC
WMQTKLINAGIRPHNNVVDVTNFVLLEYGQPLHAFDYDRFGSKEVVIRKAYENETITTLD
EEEHHHHHCCCCCCCCEEHHHHEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCEEECC
EQERTLTTEHLVITNGSSPQAVAGVMGGAESEVREDTATVLLEAAYFNGQTVRKASKDLG
CHHHEEEEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHEEEHHHCCCHHHHHHHHHCC
LRSESSVRFEKGIDPARVRLAAERAADLISRYAGGTVLEGTVEENHLDIKENVIRLSVDK
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHEEEHHH
VTKVLGMSISKEEMVNIFERLGFAVEDAERELVVTVPSRRGDIAIEEDLIEEVARLYGYD
HHHHHCCCCCHHHHHHHHHHCCCCEECCCCEEEEEECCCCCCEEEHHHHHHHHHHHCCCC
NIPSTLPEVTGFAGGLTPYQDKRRKVRRFLEGAGLSQAITYSLTNDKKATAYALEKSFKT
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHH
ILSLPMSEERSVLRHSLLPNLLDSVAYNLARQADSAAFYEIGSVFLKAEEHTKPVEKEHV
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEHHCCCCCHHHHH
AGAVTGLWHKNLWQGEKKPVDFFVVKGIVEGLLEKLGITEGIEFAQSERKELHPGRTANI
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEE
LHNGSLVGFIGQLHPSVEKELDLSDTYVFELDLHELLRLDVPEITYTPIPKYPSVTRDIA
EECCCCEEHHHHHCCCHHHCCCCCCEEEEEECHHHHHHCCCCCCCCCCCCCCCCCCCCEE
LVVDKQTTAGQLEDVIKTAGGKWLKEVHVFDVYEGEHMEEGKKSVAFSLQYLNPEQTLTE
EEEECCCCHHHHHHHHHHCCCHHHHHEEEEEEECCCHHHCCCCEEEEEEEEECCHHHCCH
EEVTKVHDQVLKALEDKYQAVLRG
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFVSYKWLQEYVNLDGMTPEILAEKITRSGIEVEGIEYKGEGIKGVVIGHVLEREQHPNA
CCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCEEHHHHHHHHCCCCCH
DKLNKCLVDIGEEEPVQIICGAPNVDKGQKVAVATVGAVLPGNFKIKKAKLRGEASNGMI
HHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEEECEECCCCEEEEEEEECCCCCCCEE
CSLQELGIQGKLVPKEYAEGIFVFPGDAETGADALQSLALDDAVLELGLTPNRADAMNML
EEHHHCCCCCCCCCHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
GVAYEVAAILGREVKLPETAYQAGAEKAADYISVKIEDPEANPLYAAKIIKDVKIGPSPL
HHHHHHHHHHCCCCCCCHHHHHCCHHHCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCC
WMQTKLINAGIRPHNNVVDVTNFVLLEYGQPLHAFDYDRFGSKEVVIRKAYENETITTLD
EEEHHHHHCCCCCCCCEEHHHHEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCEEECC
EQERTLTTEHLVITNGSSPQAVAGVMGGAESEVREDTATVLLEAAYFNGQTVRKASKDLG
CHHHEEEEEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHEEEHHHCCCHHHHHHHHHCC
LRSESSVRFEKGIDPARVRLAAERAADLISRYAGGTVLEGTVEENHLDIKENVIRLSVDK
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHEEEHHH
VTKVLGMSISKEEMVNIFERLGFAVEDAERELVVTVPSRRGDIAIEEDLIEEVARLYGYD
HHHHHCCCCCHHHHHHHHHHCCCCEECCCCEEEEEECCCCCCEEEHHHHHHHHHHHCCCC
NIPSTLPEVTGFAGGLTPYQDKRRKVRRFLEGAGLSQAITYSLTNDKKATAYALEKSFKT
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHH
ILSLPMSEERSVLRHSLLPNLLDSVAYNLARQADSAAFYEIGSVFLKAEEHTKPVEKEHV
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEHHCCCCCHHHHH
AGAVTGLWHKNLWQGEKKPVDFFVVKGIVEGLLEKLGITEGIEFAQSERKELHPGRTANI
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEE
LHNGSLVGFIGQLHPSVEKELDLSDTYVFELDLHELLRLDVPEITYTPIPKYPSVTRDIA
EECCCCEEHHHHHCCCHHHCCCCCCEEEEEECHHHHHHCCCCCCCCCCCCCCCCCCCCEE
LVVDKQTTAGQLEDVIKTAGGKWLKEVHVFDVYEGEHMEEGKKSVAFSLQYLNPEQTLTE
EEEECCCCHHHHHHHHHHCCCHHHHHEEEEEEECCCHHHCCCCEEEEEEEEECCHHHCCH
EEVTKVHDQVLKALEDKYQAVLRG
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA