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The map label for this gene is hemC

Identifier: 52786677

GI number: 52786677

Start: 2829714

End: 2830655

Strand: Reverse

Name: hemC

Synonym: BLi02945

Alternate gene names: 52786677

Gene position: 2830655-2829714 (Counterclockwise)

Preceding gene: 52786678

Following gene: 52786676

Centisome position: 67.04

GC content: 51.38

Gene sequence:

>942_bases
ATGAGAAACATCATTGTAGGCTCAAGAAGAAGCAAATTGGCGATGACCCAGACAAAATGGGTGATCAAAAAGCTGGAGGA
ATTAAATCCGGATTTCACGTTTGAAATAAAGGAAATCGTTACAAAAGGCGACCGGATATTGGATGTTACGCTGTCAAAGG
TCGGCGGAAAAGGTCTGTTCGTCAAAGAAATCGAACAGGCGATGCTCTCCGGGGAAATTGATATGGCCGTTCACAGCATG
AAGGACATGCCGTCGGTGCTTCCCGAAGGGCTGATGGTCGGCTGCATTCCAAAACGGGAAGACGCCAGGGATGTTCTCAT
TTCAAAAGGCCGTCAAAAGCTGGCTGACTTGAAACAAGGCGCAGTTGTCGGAACGAGCAGCCTCAGAAGAAGCGCACAGC
TTTTGCAAATGCGCCCCGACCTTGAGATCAAGTGGATCAGGGGAAATATCGATACAAGGCTGAAAAAACTTGAAACCGAA
GATTACGACGCGATTATTTTAGCTGCGGCCGGCCTGTCAAGAATGGGGTGGAAGGACGATGTCGTCACTGAATTTCTTGA
TCCGGAAAGCTGCCTTCCCGCCGTTGGGCAAGGCGCTTTGGCGATCGAGTGCAGAGGCTCTGATGAGGAGCTGCTGTCTC
TCTTTTCGCGCCTGAACGACCGCTACACACAGCTGACCGTCACGGCTGAGCGCGCTTTTCTTCATGCGATGGAAGGCGGC
TGCCAAGTGCCGATCGCAGGATTTGCAAAGATGGACGAAGACGGCGTCATCGAACTGACCGGTCTTGTGGCATCGCCTGA
CGGAAAAACCGTATACAGAGAAACAGTAAAAGGAAAAGATCCGGAGGCTGTCGGCAGAGAATGCGCCGCAGTCATGTCGG
AAAAAGGAGCAAAGGCACTGATTGACCGGGTGAAACAGGAGCTGGAAGACCATGAAGCATGA

Upstream 100 bases:

>100_bases
TGTTCTTTTAATCAATTTCTTTTTGCTCGGCAGCTTTTCGCAGTTTCATTGGTTTAGTTAGTTAAAACGGTTGCCCGTGA
CGGATTGGGAGGAAGAAAAC

Downstream 100 bases:

>100_bases
TCTTCCTTTAGAGGGAAAGAAAGTGCTCGTCACCCGCAATAAAGCGCAAGCCCAATCTTTCAGGGAAAAGGTGGAGGCTT
TGGGCGGGGAAGCTGTTTTA

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MRNIIVGSRRSKLAMTQTKWVIKKLEELNPDFTFEIKEIVTKGDRILDVTLSKVGGKGLFVKEIEQAMLSGEIDMAVHSM
KDMPSVLPEGLMVGCIPKREDARDVLISKGRQKLADLKQGAVVGTSSLRRSAQLLQMRPDLEIKWIRGNIDTRLKKLETE
DYDAIILAAAGLSRMGWKDDVVTEFLDPESCLPAVGQGALAIECRGSDEELLSLFSRLNDRYTQLTVTAERAFLHAMEGG
CQVPIAGFAKMDEDGVIELTGLVASPDGKTVYRETVKGKDPEAVGRECAAVMSEKGAKALIDRVKQELEDHEA

Sequences:

>Translated_313_residues
MRNIIVGSRRSKLAMTQTKWVIKKLEELNPDFTFEIKEIVTKGDRILDVTLSKVGGKGLFVKEIEQAMLSGEIDMAVHSM
KDMPSVLPEGLMVGCIPKREDARDVLISKGRQKLADLKQGAVVGTSSLRRSAQLLQMRPDLEIKWIRGNIDTRLKKLETE
DYDAIILAAAGLSRMGWKDDVVTEFLDPESCLPAVGQGALAIECRGSDEELLSLFSRLNDRYTQLTVTAERAFLHAMEGG
CQVPIAGFAKMDEDGVIELTGLVASPDGKTVYRETVKGKDPEAVGRECAAVMSEKGAKALIDRVKQELEDHEA
>Mature_313_residues
MRNIIVGSRRSKLAMTQTKWVIKKLEELNPDFTFEIKEIVTKGDRILDVTLSKVGGKGLFVKEIEQAMLSGEIDMAVHSM
KDMPSVLPEGLMVGCIPKREDARDVLISKGRQKLADLKQGAVVGTSSLRRSAQLLQMRPDLEIKWIRGNIDTRLKKLETE
DYDAIILAAAGLSRMGWKDDVVTEFLDPESCLPAVGQGALAIECRGSDEELLSLFSRLNDRYTQLTVTAERAFLHAMEGG
CQVPIAGFAKMDEDGVIELTGLVASPDGKTVYRETVKGKDPEAVGRECAAVMSEKGAKALIDRVKQELEDHEA

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=346, Percent_Identity=43.0635838150289, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI66933009, Length=346, Percent_Identity=43.0635838150289, Blast_Score=253, Evalue=2e-67,
Organism=Escherichia coli, GI48994974, Length=301, Percent_Identity=47.5083056478405, Blast_Score=254, Evalue=5e-69,
Organism=Saccharomyces cerevisiae, GI6319996, Length=317, Percent_Identity=38.801261829653, Blast_Score=199, Evalue=6e-52,
Organism=Drosophila melanogaster, GI20130425, Length=284, Percent_Identity=44.3661971830986, Blast_Score=236, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_BACLD (Q65GK1)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_080089.1
- RefSeq:   YP_092506.1
- HSSP:   P06983
- ProteinModelPortal:   Q65GK1
- SMR:   Q65GK1
- STRING:   Q65GK1
- EnsemblBacteria:   EBBACT00000055946
- EnsemblBacteria:   EBBACT00000061004
- GeneID:   3028170
- GeneID:   3098400
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02945
- KEGG:   bli:BL00625
- NMPDR:   fig|279010.5.peg.3272
- eggNOG:   COG0181
- GeneTree:   EBGT00050000001150
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- BioCyc:   BLIC279010-1:BLI02945-MONOMER
- BioCyc:   BLIC279010:BL00625-MONOMER
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34489; Mature: 34489

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNIIVGSRRSKLAMTQTKWVIKKLEELNPDFTFEIKEIVTKGDRILDVTLSKVGGKGLF
CCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHCCCEEEEEEHHHCCCCCCH
VKEIEQAMLSGEIDMAVHSMKDMPSVLPEGLMVGCIPKREDARDVLISKGRQKLADLKQG
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHCCHHHHHHHHCC
AVVGTSSLRRSAQLLQMRPDLEIKWIRGNIDTRLKKLETEDYDAIILAAAGLSRMGWKDD
CEECHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCCCCEEEEEECCHHHCCCCHH
VVTEFLDPESCLPAVGQGALAIECRGSDEELLSLFSRLNDRYTQLTVTAERAFLHAMEGG
HHHHHCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCHHEEEEEEHHHHHHHHHCCC
CQVPIAGFAKMDEDGVIELTGLVASPDGKTVYRETVKGKDPEAVGRECAAVMSEKGAKAL
CCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHH
IDRVKQELEDHEA
HHHHHHHHHHCCC
>Mature Secondary Structure
MRNIIVGSRRSKLAMTQTKWVIKKLEELNPDFTFEIKEIVTKGDRILDVTLSKVGGKGLF
CCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHCCCEEEEEEHHHCCCCCCH
VKEIEQAMLSGEIDMAVHSMKDMPSVLPEGLMVGCIPKREDARDVLISKGRQKLADLKQG
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHCCHHHHHHHHCC
AVVGTSSLRRSAQLLQMRPDLEIKWIRGNIDTRLKKLETEDYDAIILAAAGLSRMGWKDD
CEECHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCCCCEEEEEECCHHHCCCCHH
VVTEFLDPESCLPAVGQGALAIECRGSDEELLSLFSRLNDRYTQLTVTAERAFLHAMEGG
HHHHHCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCHHEEEEEEHHHHHHHHHCCC
CQVPIAGFAKMDEDGVIELTGLVASPDGKTVYRETVKGKDPEAVGRECAAVMSEKGAKAL
CCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHH
IDRVKQELEDHEA
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA