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The map label for this gene is folC [H]

Identifier: 52786669

GI number: 52786669

Start: 2819367

End: 2820671

Strand: Reverse

Name: folC [H]

Synonym: BLi02937

Alternate gene names: 52786669

Gene position: 2820671-2819367 (Counterclockwise)

Preceding gene: 52786670

Following gene: 52786668

Centisome position: 66.8

GC content: 48.89

Gene sequence:

>1305_bases
ATGCGCTTGTTCAAAACGTTTGATGAAGCACTCAGCTGGATTAACGGAAGGCTTAAATTCGGCGTGAAGCCGGGTTTGGA
ACGAATGGAATGGCTGCTGAGAAAGCTCGGGCACCCCGAGAAAAAGCTCAATGCGGTTCATGTCGCCGGAACAAACGGAA
AAGGTTCAACGATCGCTTTTCTCCGTTCGGTATTAAATGAAGCCGGTTATACAGCAGGCACCTTCACATCACCGTATATC
ATCACCTTTAACGAACGGATCAGCGTTGACGGCGCCCCGATCTCAGATGAAGAATGGACGGCGCTCTTTAATGAGGTCGG
GCCTCTTGTCCTTGAGCTTGAAAAAACCGAATTCGGAGCCGCCACAGAATTTGAAATTATTACGGCATGCGCATTTTTAT
ATTTTGCTAAATACAGGCGGACAGATTTTGTTATTTTTGAAACGGGCTTAGGCGGCCGTTTGGATTCGACAAACGTCGTG
AAGCCGATGTTAACGGTCATCACTTCGATCGGCCATGACCATACGGCGATATTAGGCGGTACATTGAAAGAGATTGCCGC
GGAAAAGGCAGGGATCATCAAACGCGGCGTTCCTGTTATCACAGCCGTTCATCAGCATGAAGCGGCTGAGGTGATAAAGC
GAAAGGCGATGGAGACAGAATCTGCTTTTATATCCCTTCACGAGGATTGCCAGCTGTACGGGGAGCTTTCATTGCCTGAG
GGAGAGCAGTTTTCGTTGAAAACACCGTTACATCATTATGAAGGAATTGAAACAAGCCTGATCGGCACCCATCAGCGGCA
AAACGCGTCTTTGGCCATACTTGCGGCCGAATGGCTGACAGCCGAAAAACATGCGGCGATCGGCGAAAAAGAGCTGAAAC
GCGGCATTCGTAACGCGGGATGGCCCGGCCGGTTTGAGAAGGTATTGAACAAGCCTCTTGTTTATATCGACGGCGCTCAT
AACAAAGAAGGGATGGAGCGGCTGGCCGAGACGATCTCCTCCCACTTCGCCGGGCGGAATATTCACGTATGCTTCAGCGC
TCTTAAAGATAAGCCGTACAGAGACATGATCCGCAAAATCGAGTCGTTTGCGTCATCGATTCACTTCACATCCTTTGATT
TCCCGAGGGCGGAATCCGCTCAAAAGCTGTTTGAGCAAAGTACCGCTGATTATAAAACATTTGATGAAGACATCGCCTCT
GTTTTCCGGTATATCGAAGAAAAAGCTGAAGAGGAAAAAGCGGTGGTCGTTATCACCGGGTCGCTTTATTTTATTTCCGC
CGTCAGGGCAGCTTTAACTGAATAA

Upstream 100 bases:

>100_bases
AAGAATCGCCGAATTGAAAAACTGATAACACGAAAAAGGAGGTGTTTTCCAGGTCTTTCTAAAGCCGCTGGAAAACACCT
TCATCAATTGAAGGGAAGGA

Downstream 100 bases:

>100_bases
AACATAAAAATGCGCTTTTGGCCACCGGCTGACGGCGCATTTTTATGTTTTAGGAAAAGAGGAGGCGCTTCATATTTGCT
AAAATAACAGCCGCTAGGCA

Product: hypothetical protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MRLFKTFDEALSWINGRLKFGVKPGLERMEWLLRKLGHPEKKLNAVHVAGTNGKGSTIAFLRSVLNEAGYTAGTFTSPYI
ITFNERISVDGAPISDEEWTALFNEVGPLVLELEKTEFGAATEFEIITACAFLYFAKYRRTDFVIFETGLGGRLDSTNVV
KPMLTVITSIGHDHTAILGGTLKEIAAEKAGIIKRGVPVITAVHQHEAAEVIKRKAMETESAFISLHEDCQLYGELSLPE
GEQFSLKTPLHHYEGIETSLIGTHQRQNASLAILAAEWLTAEKHAAIGEKELKRGIRNAGWPGRFEKVLNKPLVYIDGAH
NKEGMERLAETISSHFAGRNIHVCFSALKDKPYRDMIRKIESFASSIHFTSFDFPRAESAQKLFEQSTADYKTFDEDIAS
VFRYIEEKAEEEKAVVVITGSLYFISAVRAALTE

Sequences:

>Translated_434_residues
MRLFKTFDEALSWINGRLKFGVKPGLERMEWLLRKLGHPEKKLNAVHVAGTNGKGSTIAFLRSVLNEAGYTAGTFTSPYI
ITFNERISVDGAPISDEEWTALFNEVGPLVLELEKTEFGAATEFEIITACAFLYFAKYRRTDFVIFETGLGGRLDSTNVV
KPMLTVITSIGHDHTAILGGTLKEIAAEKAGIIKRGVPVITAVHQHEAAEVIKRKAMETESAFISLHEDCQLYGELSLPE
GEQFSLKTPLHHYEGIETSLIGTHQRQNASLAILAAEWLTAEKHAAIGEKELKRGIRNAGWPGRFEKVLNKPLVYIDGAH
NKEGMERLAETISSHFAGRNIHVCFSALKDKPYRDMIRKIESFASSIHFTSFDFPRAESAQKLFEQSTADYKTFDEDIAS
VFRYIEEKAEEEKAVVVITGSLYFISAVRAALTE
>Mature_434_residues
MRLFKTFDEALSWINGRLKFGVKPGLERMEWLLRKLGHPEKKLNAVHVAGTNGKGSTIAFLRSVLNEAGYTAGTFTSPYI
ITFNERISVDGAPISDEEWTALFNEVGPLVLELEKTEFGAATEFEIITACAFLYFAKYRRTDFVIFETGLGGRLDSTNVV
KPMLTVITSIGHDHTAILGGTLKEIAAEKAGIIKRGVPVITAVHQHEAAEVIKRKAMETESAFISLHEDCQLYGELSLPE
GEQFSLKTPLHHYEGIETSLIGTHQRQNASLAILAAEWLTAEKHAAIGEKELKRGIRNAGWPGRFEKVLNKPLVYIDGAH
NKEGMERLAETISSHFAGRNIHVCFSALKDKPYRDMIRKIESFASSIHFTSFDFPRAESAQKLFEQSTADYKTFDEDIAS
VFRYIEEKAEEEKAVVVITGSLYFISAVRAALTE

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=345, Percent_Identity=32.7536231884058, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI66932990, Length=345, Percent_Identity=32.7536231884058, Blast_Score=159, Evalue=4e-39,
Organism=Escherichia coli, GI1788654, Length=410, Percent_Identity=29.2682926829268, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17553150, Length=439, Percent_Identity=27.5626423690205, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17553148, Length=439, Percent_Identity=27.5626423690205, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI71984923, Length=439, Percent_Identity=27.5626423690205, Blast_Score=144, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6323760, Length=433, Percent_Identity=29.0993071593533, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6324815, Length=333, Percent_Identity=31.8318318318318, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6322718, Length=295, Percent_Identity=23.728813559322, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24641571, Length=295, Percent_Identity=36.271186440678, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24581568, Length=199, Percent_Identity=33.6683417085427, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 48393; Mature: 48393

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFKTFDEALSWINGRLKFGVKPGLERMEWLLRKLGHPEKKLNAVHVAGTNGKGSTIAF
CCHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCHHHCCEEEEECCCCCCHHHHH
LRSVLNEAGYTAGTFTSPYIITFNERISVDGAPISDEEWTALFNEVGPLVLELEKTEFGA
HHHHHHHCCCCCCCCCCCEEEEECCEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCC
ATEFEIITACAFLYFAKYRRTDFVIFETGLGGRLDSTNVVKPMLTVITSIGHDHTAILGG
CCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
TLKEIAAEKAGIIKRGVPVITAVHQHEAAEVIKRKAMETESAFISLHEDCQLYGELSLPE
HHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GEQFSLKTPLHHYEGIETSLIGTHQRQNASLAILAAEWLTAEKHAAIGEKELKRGIRNAG
CCCEEECCCHHHHCCCCHHHCCCCCCCCCCEEEEEHHHHHHHHHHHCCHHHHHHHHHCCC
WPGRFEKVLNKPLVYIDGAHNKEGMERLAETISSHFAGRNIHVCFSALKDKPYRDMIRKI
CCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHH
ESFASSIHFTSFDFPRAESAQKLFEQSTADYKTFDEDIASVFRYIEEKAEEEKAVVVITG
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
SLYFISAVRAALTE
HHHHHHHHHHHHCC
>Mature Secondary Structure
MRLFKTFDEALSWINGRLKFGVKPGLERMEWLLRKLGHPEKKLNAVHVAGTNGKGSTIAF
CCHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCHHHCCEEEEECCCCCCHHHHH
LRSVLNEAGYTAGTFTSPYIITFNERISVDGAPISDEEWTALFNEVGPLVLELEKTEFGA
HHHHHHHCCCCCCCCCCCEEEEECCEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCC
ATEFEIITACAFLYFAKYRRTDFVIFETGLGGRLDSTNVVKPMLTVITSIGHDHTAILGG
CCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
TLKEIAAEKAGIIKRGVPVITAVHQHEAAEVIKRKAMETESAFISLHEDCQLYGELSLPE
HHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GEQFSLKTPLHHYEGIETSLIGTHQRQNASLAILAAEWLTAEKHAAIGEKELKRGIRNAG
CCCEEECCCHHHHCCCCHHHCCCCCCCCCCEEEEEHHHHHHHHHHHCCHHHHHHHHHCCC
WPGRFEKVLNKPLVYIDGAHNKEGMERLAETISSHFAGRNIHVCFSALKDKPYRDMIRKI
CCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHH
ESFASSIHFTSFDFPRAESAQKLFEQSTADYKTFDEDIASVFRYIEEKAEEEKAVVVITG
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
SLYFISAVRAALTE
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]