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The map label for this gene is nadB [H]

Identifier: 52786646

GI number: 52786646

Start: 2801021

End: 2802595

Strand: Reverse

Name: nadB [H]

Synonym: BLi02914

Alternate gene names: 52786646

Gene position: 2802595-2801021 (Counterclockwise)

Preceding gene: 52786649

Following gene: 52786645

Centisome position: 66.37

GC content: 50.54

Gene sequence:

>1575_bases
ATGTCTCATCAGACGATTATTGTTGTCGGAGCAGGTGCCGCAGCGTTGTCTTTTGCTGCATCCATTCCGCCGTCTTATGA
AGTGGTTGTGATGACAAAAGCATCTGTAAAAGACAGCAATTCAACCCTCGCTCAGGGAGGAATTGCATCTGCTGTATTGC
CTCGCGATTCCTGGTCGCTTCATGCGGACGACACGTTGTATGCCGGGTGCGGCCACAATCAAATCGAAGCGGTCCATGAT
GCCATTAAAGACGGACAGCGCCTTGTTGATGAGCTGATCCTTCAGCATTTCCCTTTTGATACTGACGAATCAGGAAAGCT
GAGCCTCGGAAAAGAAGGCGCGCATTCGGTGAACAGAATTTTGCATGCCGGGGGAGACGCAACAGGACAGGAGTTGATCC
GGCACTTAATCGGACGGCTCGGTCCGCACGTCGTGCTGAAAGAATACGAAACGGCGGCGGATTTATGGATTGAAGACGGA
CGCTGCTGTGGCGTCTGGACGAAAGACAGCCGTGGGAAGACGTTTCTCAGAAAAGCCGATTTTGTCGTGCTGGCGGCAGG
AGGCTGCGGAAATCTATTTCAAATCAACACGAATAACCGTTCTGTGACAAGCGATGCGATTTCTTTGGCGTATCGGGCAG
GAGCAGAGCTCGCAGACTTGGAATTCGTTCAATTTCATCCTACATTGCTTGTCAAAGACGGGCGCGCGCACGGACTTGTT
TCAGAAGCTGTGCGCGGAGAAGGTGCTGTGTTGATTGATGAAAACGGACAGCGGGTGATGGAAGGCATACATTCTTTGCT
GGATTTAGCGCCGCGGGACGTTGTATCTCGGGCGATACACCAAAAGCTTGCCTGCGGAAGCAGCGTATTTTTAGATATTT
CCCGCATATCAGACTTTAAAAGCCGCTTCCCTACGATTTCTTCTCTTTGCGAAAAAGCCGGTATTTCCATTGAAGAGGGC
CGGATTCCGGTTTCACCGGGCATGCATTTTTTAATGGGGGGCGTTTCTGTGAATAAATGGGGAGAAACGGCTGTTCCAAA
CCTTTTTGTGATCGGGGAAGCCGCTTGCACCGGCTTTCACGGTGCCAACCGTCTGGCAAGCAATTCTCTCTTGGAATGCA
TCGTCTTCGGAAGGAGAGCGGCGGAGCGGATTCAAGCCTGCCAAAAACCGGAGCCTGTCAGAGGCCAATCTCCTCAGCCT
GAGAATGTGACGTATTCTGTTCCTTTGACAGATGTGAAGGATATTCAGAAACAAATGACAGATTATGCGGCGATCGTGCG
TACGGAGGATGAATTGACGAAGGTGAAAGAATGGCTTGAGCGAATGCCGGTTCAAATCGTAAATGTGAAAGAAATCACAA
TAGAACAGCTCGAGCTAGCTCATTTATGGCAGACTGCCAAACAGATGACGGCCTCGGCACTGCTTCGTACAGAAAGCAGG
GGCGGCCATTACCGTTCGGATTGTCCGGCGCCTGATGATAAAAACTGGCACGGCAAGCAAATCGTTCACACAAAAGGCGG
GATCAAGATCAGACAAAACGAAGGGATTGTGAAATATGCAGCGGTTCATACTTAG

Upstream 100 bases:

>100_bases
GGCGCTCGTCTCCCATTCTTTATAAAAAATACTTGAGTTTATTTTATCGATGTGTAAATATAGGTGTCAAGACACATGTA
AATTTACAGGAGGAGTCGAC

Downstream 100 bases:

>100_bases
GAACATGCTTAAGGAATTTTTCAAGGAAGATATCGGCCGGGGGGATCTCACTTCAGAAGCCGTTTTTGATGACAATCATC
AATGCGAAGCTGTCATCACA

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MSHQTIIVVGAGAAALSFAASIPPSYEVVVMTKASVKDSNSTLAQGGIASAVLPRDSWSLHADDTLYAGCGHNQIEAVHD
AIKDGQRLVDELILQHFPFDTDESGKLSLGKEGAHSVNRILHAGGDATGQELIRHLIGRLGPHVVLKEYETAADLWIEDG
RCCGVWTKDSRGKTFLRKADFVVLAAGGCGNLFQINTNNRSVTSDAISLAYRAGAELADLEFVQFHPTLLVKDGRAHGLV
SEAVRGEGAVLIDENGQRVMEGIHSLLDLAPRDVVSRAIHQKLACGSSVFLDISRISDFKSRFPTISSLCEKAGISIEEG
RIPVSPGMHFLMGGVSVNKWGETAVPNLFVIGEAACTGFHGANRLASNSLLECIVFGRRAAERIQACQKPEPVRGQSPQP
ENVTYSVPLTDVKDIQKQMTDYAAIVRTEDELTKVKEWLERMPVQIVNVKEITIEQLELAHLWQTAKQMTASALLRTESR
GGHYRSDCPAPDDKNWHGKQIVHTKGGIKIRQNEGIVKYAAVHT

Sequences:

>Translated_524_residues
MSHQTIIVVGAGAAALSFAASIPPSYEVVVMTKASVKDSNSTLAQGGIASAVLPRDSWSLHADDTLYAGCGHNQIEAVHD
AIKDGQRLVDELILQHFPFDTDESGKLSLGKEGAHSVNRILHAGGDATGQELIRHLIGRLGPHVVLKEYETAADLWIEDG
RCCGVWTKDSRGKTFLRKADFVVLAAGGCGNLFQINTNNRSVTSDAISLAYRAGAELADLEFVQFHPTLLVKDGRAHGLV
SEAVRGEGAVLIDENGQRVMEGIHSLLDLAPRDVVSRAIHQKLACGSSVFLDISRISDFKSRFPTISSLCEKAGISIEEG
RIPVSPGMHFLMGGVSVNKWGETAVPNLFVIGEAACTGFHGANRLASNSLLECIVFGRRAAERIQACQKPEPVRGQSPQP
ENVTYSVPLTDVKDIQKQMTDYAAIVRTEDELTKVKEWLERMPVQIVNVKEITIEQLELAHLWQTAKQMTASALLRTESR
GGHYRSDCPAPDDKNWHGKQIVHTKGGIKIRQNEGIVKYAAVHT
>Mature_523_residues
SHQTIIVVGAGAAALSFAASIPPSYEVVVMTKASVKDSNSTLAQGGIASAVLPRDSWSLHADDTLYAGCGHNQIEAVHDA
IKDGQRLVDELILQHFPFDTDESGKLSLGKEGAHSVNRILHAGGDATGQELIRHLIGRLGPHVVLKEYETAADLWIEDGR
CCGVWTKDSRGKTFLRKADFVVLAAGGCGNLFQINTNNRSVTSDAISLAYRAGAELADLEFVQFHPTLLVKDGRAHGLVS
EAVRGEGAVLIDENGQRVMEGIHSLLDLAPRDVVSRAIHQKLACGSSVFLDISRISDFKSRFPTISSLCEKAGISIEEGR
IPVSPGMHFLMGGVSVNKWGETAVPNLFVIGEAACTGFHGANRLASNSLLECIVFGRRAAERIQACQKPEPVRGQSPQPE
NVTYSVPLTDVKDIQKQMTDYAAIVRTEDELTKVKEWLERMPVQIVNVKEITIEQLELAHLWQTAKQMTASALLRTESRG
GHYRSDCPAPDDKNWHGKQIVHTKGGIKIRQNEGIVKYAAVHT

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=532, Percent_Identity=31.015037593985, Blast_Score=190, Evalue=3e-48,
Organism=Escherichia coli, GI1788928, Length=514, Percent_Identity=36.5758754863813, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1790597, Length=532, Percent_Identity=28.9473684210526, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1786942, Length=526, Percent_Identity=31.3688212927757, Blast_Score=179, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17505833, Length=520, Percent_Identity=31.1538461538462, Blast_Score=187, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI17550100, Length=526, Percent_Identity=31.7490494296578, Blast_Score=184, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6322416, Length=540, Percent_Identity=30.9259259259259, Blast_Score=200, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6322701, Length=534, Percent_Identity=30.1498127340824, Blast_Score=194, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6320788, Length=483, Percent_Identity=27.3291925465839, Blast_Score=113, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6322511, Length=314, Percent_Identity=25.796178343949, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17137288, Length=552, Percent_Identity=31.7028985507246, Blast_Score=198, Evalue=7e-51,
Organism=Drosophila melanogaster, GI24655642, Length=552, Percent_Identity=31.7028985507246, Blast_Score=198, Evalue=7e-51,
Organism=Drosophila melanogaster, GI24655647, Length=552, Percent_Identity=31.7028985507246, Blast_Score=198, Evalue=7e-51,
Organism=Drosophila melanogaster, GI24663005, Length=530, Percent_Identity=30.7547169811321, Blast_Score=182, Evalue=7e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 56911; Mature: 56780

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHQTIIVVGAGAAALSFAASIPPSYEVVVMTKASVKDSNSTLAQGGIASAVLPRDSWSL
CCCCEEEEEECCHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHCCCCHHEECCCCCCCC
HADDTLYAGCGHNQIEAVHDAIKDGQRLVDELILQHFPFDTDESGKLSLGKEGAHSVNRI
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHH
LHAGGDATGQELIRHLIGRLGPHVVLKEYETAADLWIEDGRCCGVWTKDSRGKTFLRKAD
HHCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHEEECCCEEEEECCCCCCCCCEEECC
FVVLAAGGCGNLFQINTNNRSVTSDAISLAYRAGAELADLEFVQFHPTLLVKDGRAHGLV
EEEEEECCCCCEEEEECCCCEEHHHHHHHHHHCCCCHHCCEEEEECCEEEEECCCCCCHH
SEAVRGEGAVLIDENGQRVMEGIHSLLDLAPRDVVSRAIHQKLACGSSVFLDISRISDFK
HHHHCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHEEEHHHHHHHH
SRFPTISSLCEKAGISIEEGRIPVSPGMHFLMGGVSVNKWGETAVPNLFVIGEAACTGFH
HHCCCHHHHHHHHCCEEECCCCCCCCCHHHHCCCCEECCCCCCCCCCEEEEECHHHCCCC
GANRLASNSLLECIVFGRRAAERIQACQKPEPVRGQSPQPENVTYSVPLTDVKDIQKQMT
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHH
DYAAIVRTEDELTKVKEWLERMPVQIVNVKEITIEQLELAHLWQTAKQMTASALLRTESR
HHHHHHCCHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GGHYRSDCPAPDDKNWHGKQIVHTKGGIKIRQNEGIVKYAAVHT
CCCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCEEEEEEECC
>Mature Secondary Structure 
SHQTIIVVGAGAAALSFAASIPPSYEVVVMTKASVKDSNSTLAQGGIASAVLPRDSWSL
CCCEEEEEECCHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHCCCCHHEECCCCCCCC
HADDTLYAGCGHNQIEAVHDAIKDGQRLVDELILQHFPFDTDESGKLSLGKEGAHSVNRI
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHH
LHAGGDATGQELIRHLIGRLGPHVVLKEYETAADLWIEDGRCCGVWTKDSRGKTFLRKAD
HHCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHEEECCCEEEEECCCCCCCCCEEECC
FVVLAAGGCGNLFQINTNNRSVTSDAISLAYRAGAELADLEFVQFHPTLLVKDGRAHGLV
EEEEEECCCCCEEEEECCCCEEHHHHHHHHHHCCCCHHCCEEEEECCEEEEECCCCCCHH
SEAVRGEGAVLIDENGQRVMEGIHSLLDLAPRDVVSRAIHQKLACGSSVFLDISRISDFK
HHHHCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHEEEHHHHHHHH
SRFPTISSLCEKAGISIEEGRIPVSPGMHFLMGGVSVNKWGETAVPNLFVIGEAACTGFH
HHCCCHHHHHHHHCCEEECCCCCCCCCHHHHCCCCEECCCCCCCCCCEEEEECHHHCCCC
GANRLASNSLLECIVFGRRAAERIQACQKPEPVRGQSPQPENVTYSVPLTDVKDIQKQMT
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHH
DYAAIVRTEDELTKVKEWLERMPVQIVNVKEITIEQLELAHLWQTAKQMTASALLRTESR
HHHHHHCCHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GGHYRSDCPAPDDKNWHGKQIVHTKGGIKIRQNEGIVKYAAVHT
CCCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8444804; 9384377 [H]