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The map label for this gene is greA

Identifier: 52786594

GI number: 52786594

Start: 2749306

End: 2749782

Strand: Reverse

Name: greA

Synonym: BLi02860

Alternate gene names: 52786594

Gene position: 2749782-2749306 (Counterclockwise)

Preceding gene: 52786595

Following gene: 52786593

Centisome position: 65.12

GC content: 43.19

Gene sequence:

>477_bases
ATGGCACAAGAGAAAGTATTTCCTATGACAGAAACGGGAAAGAAAAAACTGGAAGAAGAACTTGAGTATTTGAAAACGGT
TAAACGTAAAGAAGTTGTTGAACGTATTAAAGTGGCAAGAAGCTTCGGAGACCTTTCCGAAAACTCCGAATACGATTCAG
CAAAAGAAGAACAAGCTTTTGTAGAAGGGCGCATCACAACCCTTGACAATATGATCCGCAACGCAAAGATCATCGAAGAT
GACGGTGCAAACTCAGACATCGTCAGCCTTGGAAAAACCGTTACATTTACGGAGCTTCCTGACGGTGAAGAAGAATCTTA
TACGATTGTGGGCAGCGCGGAAGCCGATCCGTTTGAAGGAAAAATCTCGAACGATTCCCCAATCGCGAAAAGCCTTCTCG
GCAAGCAGGTTGGAGACGAAGTAACCGTTCAAACACCTGGCGGCGAAATGCTTGTAAAAATTGTGAAAATTTCATAA

Upstream 100 bases:

>100_bases
GGAGAGAAGAAGGGGACAAGGGTTGTCCTCATTCTTGTATATTCAAGTCTATACTCTCACATACAAGTGATGAGGAACCA
ACTAGAAGGAGTGAAGGAAC

Downstream 100 bases:

>100_bases
AATTTGTTTAGCAAACGATGACCTCGTCCACAATGTGGATGAGGTGTTTTTATAATGAAATGGCAAAAGCGTATGAAGTG
GGTATCGATTATCATTTCGG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MAQEKVFPMTETGKKKLEEELEYLKTVKRKEVVERIKVARSFGDLSENSEYDSAKEEQAFVEGRITTLDNMIRNAKIIED
DGANSDIVSLGKTVTFTELPDGEEESYTIVGSAEADPFEGKISNDSPIAKSLLGKQVGDEVTVQTPGGEMLVKIVKIS

Sequences:

>Translated_158_residues
MAQEKVFPMTETGKKKLEEELEYLKTVKRKEVVERIKVARSFGDLSENSEYDSAKEEQAFVEGRITTLDNMIRNAKIIED
DGANSDIVSLGKTVTFTELPDGEEESYTIVGSAEADPFEGKISNDSPIAKSLLGKQVGDEVTVQTPGGEMLVKIVKIS
>Mature_157_residues
AQEKVFPMTETGKKKLEEELEYLKTVKRKEVVERIKVARSFGDLSENSEYDSAKEEQAFVEGRITTLDNMIRNAKIIEDD
GANSDIVSLGKTVTFTELPDGEEESYTIVGSAEADPFEGKISNDSPIAKSLLGKQVGDEVTVQTPGGEMLVKIVKIS

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=154, Percent_Identity=44.1558441558442, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI87082261, Length=141, Percent_Identity=36.8794326241135, Blast_Score=81, Evalue=3e-17,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_BACLD (Q65GT4)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_080004.1
- RefSeq:   YP_092423.1
- HSSP:   P0A6W5
- ProteinModelPortal:   Q65GT4
- SMR:   Q65GT4
- STRING:   Q65GT4
- EnsemblBacteria:   EBBACT00000055641
- EnsemblBacteria:   EBBACT00000058495
- GeneID:   3027877
- GeneID:   3098130
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02860
- KEGG:   bli:BL02024
- NMPDR:   fig|279010.5.peg.3151
- eggNOG:   COG0782
- GeneTree:   EBGT00050000001871
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   BLIC279010-1:BLI02860-MONOMER
- BioCyc:   BLIC279010:BL02024-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17406; Mature: 17275

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQEKVFPMTETGKKKLEEELEYLKTVKRKEVVERIKVARSFGDLSENSEYDSAKEEQAF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
VEGRITTLDNMIRNAKIIEDDGANSDIVSLGKTVTFTELPDGEEESYTIVGSAEADPFEG
HHHHHHHHHHHHHCCEEEECCCCCCHHHHCCCEEEEEECCCCCCCCEEEEECCCCCCCCC
KISNDSPIAKSLLGKQVGDEVTVQTPGGEMLVKIVKIS
CCCCCCHHHHHHHHHHCCCCEEEECCCHHEEEEEEECC
>Mature Secondary Structure 
AQEKVFPMTETGKKKLEEELEYLKTVKRKEVVERIKVARSFGDLSENSEYDSAKEEQAF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
VEGRITTLDNMIRNAKIIEDDGANSDIVSLGKTVTFTELPDGEEESYTIVGSAEADPFEG
HHHHHHHHHHHHHCCEEEECCCCCCHHHHCCCEEEEEECCCCCCCCEEEEECCCCCCCCC
KISNDSPIAKSLLGKQVGDEVTVQTPGGEMLVKIVKIS
CCCCCCHHHHHHHHHHCCCCEEEECCCHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA