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The map label for this gene is sacC [H]

Identifier: 52786561

GI number: 52786561

Start: 2712642

End: 2714675

Strand: Reverse

Name: sacC [H]

Synonym: BLi02827

Alternate gene names: 52786561

Gene position: 2714675-2712642 (Counterclockwise)

Preceding gene: 52786562

Following gene: 52786558

Centisome position: 64.29

GC content: 49.95

Gene sequence:

>2034_bases
ATGAAAAAGAGAATGATTCAGATGGGGATCATAGGGGCTATGATGTTCCCGGAAGCCTTTTCCGCAGCTGCTGCCGACCC
GGACTACTATAACGAGGATCATCGGCCCAAGTATCATTTCACGCCGGAAGCAAACTGGATGAATGACCCGAACGGGATGG
TGTATTACGCGGGGGAATATCATCTGTTTTATCAATATCATCCATATGGCTTGCGCTGGGGGCCAATGCATTGGGGGCAC
GCGGTAAGCAAAGATTTAGTCAAGTGGGAGCATTTGCCGGTCGCACTGTATCCGGATGAAAAAGGAACGATTTTTTCGGG
CAGCGCTGTCGCCGATCGGCATAATACTACAGGGTTTCAAACAGGGACGGAGAAGCCGCTCGTTGCCATTTATACGCAGG
ACCGGAGCGGGGAACAAGTGCAAAGCATCGCCTACAGCAATGACAAAGGCAGGACATGGACGAAGTACTCAGGCAACCCT
GTCATTCCAAATCCGGGAAAAAGAGATTTCCGCGATCCGAAAGTCATTTGGCATGAACAGACAAAAAAGTGGGTGATGTT
GCTTGCGGGGGGCGATCGAATTCTGATCTATACATCGCCTGACTTGAAGCGGTGGACATATGCGAGCGAATTCGGCGAAG
GAGAGGGCAGCCATGGAGGCGTGTGGGAATGTCCGGATCTGTTTGAGCTTCCTGTAGAGGGCAGGCCGAATGAAACGAAA
TGGGTGATGCAAGTCAGTGTTGGAGACGGTGCTGTCTCAGGAGGATCAGGCATGCAATATTTCGTCGGCAGTTTTGACGG
AACAACCTTTAAAAATGAAAACCCGCCGAATCGCGTACTATGGACAGATTACGGCAAAGACTTCTACGCAGCTGTCAGCT
GGTCTGATATCCCGAAGTCAGACGGACGAAGGCTTTGGTTAGGGTGGATGAGCAATTGGCAATACGCAAACGATGTTCCC
ACTTCCCCGTGGAGAAGCGCAATGTCGATCCCGAGAGAAGTAAAATTGAAAGCGTTTTCCGAAGGGCTTAGAATGATTCA
AGCTCCGGTAGCGGAGCTGCAGTCGATACGGGGAGCATCGCAAACGTGGAAAAACAAGATCATTTCGCCTCGAAACGGAA
ATTTGCTGAAAGGGCTGTCAGGCGATGCTTATGAAATCAATGCGGAATTTCAAGTAAATACCGGAGCTGCGGCCGAATTC
GGCTTTAAAGTCCGAACAGGGGAAAACGAGTATACGAAAATCGGCTACAGCAAAAACAGCGCTTCACTGTTCGTCGACCG
GAGCCAATCAGGAAACGTCTCCTTTAATCCGAACTTTAACACTGGAAAGCATGCGGCCCCGCTGGAACCGGTCGCTGGGA
AGGTGAAGATGCGCATTTATGTCGACCGCTCTTCAGTCGAAGTATTCGGCAACGACGGGAGGCAGGTGATCACCGACATT
ATTTTGCCGGACCAATCCAGCAAAGGGCTAGAAGTATACGCATCAAACGGGTTTGTCAAGCTGAATTCCATGACGGTTCA
TCCTCTGAAAAAAGTATGGGGAGCGTCGCCCTTTCAATCAAATTTGACCGGCTGGACGACCGTAAACGGTGTATGGGCCG
ATACGATTGATGGAAAACAAGGGCGCTCTGAAGGAGATTCGTTCATTTTATCTTCAGCAAAAGCAACAGATTTCACATAT
GAAGCAGATGTCGCGATAAAGGATGGAAATGGAAGAGGGGCAGGGGCTCTCGTGTTTCGGGCCGATCAGGATGTTAAAAA
CGGATACCTTGCCAATGTGGATGCCAAGCATGACGTGGTGAAGTTTTTCAAATTTGAAGACGGCTCGGCCTCTGTTATTG
CCGAACACCGCACACCGATAGAAACCGGCCGGAAATATCACCTGAAAACGGTGGCCCGCGGTGCAAACTTTTATATTTAC
CTTGATGATCAATTGGTGATAAGCGCCCGGGATTCAACGTTTGCCGATGGAGCCTTCGGTTTGAATGCATGGGATGCGAC
CGCAGTCTTCCAACATGTTTATGCCAAACGATGA

Upstream 100 bases:

>100_bases
CTTAGCTTTTGAGAAGCAGGTCCATTCGGCCGCAAAAAAGGGGGCGGCTGTTTGGACTTCCTGCCTAAAAAACCGAGTGA
AGAAGAGGGGGGAAAAAGTC

Downstream 100 bases:

>100_bases
TCATGTGGCTGGAATGCCGGGCATTCCAGCCTTTCCTTTAAAAGACGCTGCGGACCAATCCGCCGTCCGCACGCAAGGCT
GCACCATTGATCGGCGAAGA

Product: SacC

Products: NA

Alternate protein names: Beta-D-fructofuranosidase; Exo-beta-D-fructosidase; Exo-levanase [H]

Number of amino acids: Translated: 677; Mature: 677

Protein sequence:

>677_residues
MKKRMIQMGIIGAMMFPEAFSAAAADPDYYNEDHRPKYHFTPEANWMNDPNGMVYYAGEYHLFYQYHPYGLRWGPMHWGH
AVSKDLVKWEHLPVALYPDEKGTIFSGSAVADRHNTTGFQTGTEKPLVAIYTQDRSGEQVQSIAYSNDKGRTWTKYSGNP
VIPNPGKRDFRDPKVIWHEQTKKWVMLLAGGDRILIYTSPDLKRWTYASEFGEGEGSHGGVWECPDLFELPVEGRPNETK
WVMQVSVGDGAVSGGSGMQYFVGSFDGTTFKNENPPNRVLWTDYGKDFYAAVSWSDIPKSDGRRLWLGWMSNWQYANDVP
TSPWRSAMSIPREVKLKAFSEGLRMIQAPVAELQSIRGASQTWKNKIISPRNGNLLKGLSGDAYEINAEFQVNTGAAAEF
GFKVRTGENEYTKIGYSKNSASLFVDRSQSGNVSFNPNFNTGKHAAPLEPVAGKVKMRIYVDRSSVEVFGNDGRQVITDI
ILPDQSSKGLEVYASNGFVKLNSMTVHPLKKVWGASPFQSNLTGWTTVNGVWADTIDGKQGRSEGDSFILSSAKATDFTY
EADVAIKDGNGRGAGALVFRADQDVKNGYLANVDAKHDVVKFFKFEDGSASVIAEHRTPIETGRKYHLKTVARGANFYIY
LDDQLVISARDSTFADGAFGLNAWDATAVFQHVYAKR

Sequences:

>Translated_677_residues
MKKRMIQMGIIGAMMFPEAFSAAAADPDYYNEDHRPKYHFTPEANWMNDPNGMVYYAGEYHLFYQYHPYGLRWGPMHWGH
AVSKDLVKWEHLPVALYPDEKGTIFSGSAVADRHNTTGFQTGTEKPLVAIYTQDRSGEQVQSIAYSNDKGRTWTKYSGNP
VIPNPGKRDFRDPKVIWHEQTKKWVMLLAGGDRILIYTSPDLKRWTYASEFGEGEGSHGGVWECPDLFELPVEGRPNETK
WVMQVSVGDGAVSGGSGMQYFVGSFDGTTFKNENPPNRVLWTDYGKDFYAAVSWSDIPKSDGRRLWLGWMSNWQYANDVP
TSPWRSAMSIPREVKLKAFSEGLRMIQAPVAELQSIRGASQTWKNKIISPRNGNLLKGLSGDAYEINAEFQVNTGAAAEF
GFKVRTGENEYTKIGYSKNSASLFVDRSQSGNVSFNPNFNTGKHAAPLEPVAGKVKMRIYVDRSSVEVFGNDGRQVITDI
ILPDQSSKGLEVYASNGFVKLNSMTVHPLKKVWGASPFQSNLTGWTTVNGVWADTIDGKQGRSEGDSFILSSAKATDFTY
EADVAIKDGNGRGAGALVFRADQDVKNGYLANVDAKHDVVKFFKFEDGSASVIAEHRTPIETGRKYHLKTVARGANFYIY
LDDQLVISARDSTFADGAFGLNAWDATAVFQHVYAKR
>Mature_677_residues
MKKRMIQMGIIGAMMFPEAFSAAAADPDYYNEDHRPKYHFTPEANWMNDPNGMVYYAGEYHLFYQYHPYGLRWGPMHWGH
AVSKDLVKWEHLPVALYPDEKGTIFSGSAVADRHNTTGFQTGTEKPLVAIYTQDRSGEQVQSIAYSNDKGRTWTKYSGNP
VIPNPGKRDFRDPKVIWHEQTKKWVMLLAGGDRILIYTSPDLKRWTYASEFGEGEGSHGGVWECPDLFELPVEGRPNETK
WVMQVSVGDGAVSGGSGMQYFVGSFDGTTFKNENPPNRVLWTDYGKDFYAAVSWSDIPKSDGRRLWLGWMSNWQYANDVP
TSPWRSAMSIPREVKLKAFSEGLRMIQAPVAELQSIRGASQTWKNKIISPRNGNLLKGLSGDAYEINAEFQVNTGAAAEF
GFKVRTGENEYTKIGYSKNSASLFVDRSQSGNVSFNPNFNTGKHAAPLEPVAGKVKMRIYVDRSSVEVFGNDGRQVITDI
ILPDQSSKGLEVYASNGFVKLNSMTVHPLKKVWGASPFQSNLTGWTTVNGVWADTIDGKQGRSEGDSFILSSAKATDFTY
EADVAIKDGNGRGAGALVFRADQDVKNGYLANVDAKHDVVKFFKFEDGSASVIAEHRTPIETGRKYHLKTVARGANFYIY
LDDQLVISARDSTFADGAFGLNAWDATAVFQHVYAKR

Specific function: Exo-fructosidase that can hydrolyze both levan and inulin, leading to the production of free fructose. Is also able to hydrolyze sucrose and to a small extent raffinose, but not melezitose, stachylose, cellobiose, maltose, and lactose [H]

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=477, Percent_Identity=37.1069182389937, Blast_Score=276, Evalue=8e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR010496
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR011040 [H]

Pfam domain/function: PF06439 DUF1080; PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.80 [H]

Molecular weight: Translated: 75479; Mature: 75479

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRMIQMGIIGAMMFPEAFSAAAADPDYYNEDHRPKYHFTPEANWMNDPNGMVYYAGEY
CCCCEEEHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEECEE
HLFYQYHPYGLRWGPMHWGHAVSKDLVKWEHLPVALYPDEKGTIFSGSAVADRHNTTGFQ
EEEEEECCCCEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEECCEECCCCCCCCCC
TGTEKPLVAIYTQDRSGEQVQSIAYSNDKGRTWTKYSGNPVIPNPGKRDFRDPKVIWHEQ
CCCCCCEEEEEECCCCCCHHHHHEECCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEECC
TKKWVMLLAGGDRILIYTSPDLKRWTYASEFGEGEGSHGGVWECPDLFELPVEGRPNETK
CCEEEEEEECCCEEEEEECCCCCCEEEHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCE
WVMQVSVGDGAVSGGSGMQYFVGSFDGTTFKNENPPNRVLWTDYGKDFYAAVSWSDIPKS
EEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCC
DGRRLWLGWMSNWQYANDVPTSPWRSAMSIPREVKLKAFSEGLRMIQAPVAELQSIRGAS
CCCEEEEEECCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
QTWKNKIISPRNGNLLKGLSGDAYEINAEFQVNTGAAAEFGFKVRTGENEYTKIGYSKNS
HHHHHCCCCCCCCCEEECCCCCEEEEEEEEEECCCCCEECCEEEECCCCCEEEEEECCCC
ASLFVDRSQSGNVSFNPNFNTGKHAAPLEPVAGKVKMRIYVDRSSVEVFGNDGRQVITDI
CEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEECCCCCEEEEEE
ILPDQSSKGLEVYASNGFVKLNSMTVHPLKKVWGASPFQSNLTGWTTVNGVWADTIDGKQ
ECCCCCCCCEEEEECCCEEEEECEEECHHHHHHCCCCCCCCCCCEEEECCEEEECCCCCC
GRSEGDSFILSSAKATDFTYEADVAIKDGNGRGAGALVFRADQDVKNGYLANVDAKHDVV
CCCCCCEEEEECCCCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCCCEEEECCCCCCEE
KFFKFEDGSASVIAEHRTPIETGRKYHLKTVARGANFYIYLDDQLVISARDSTFADGAFG
EEEEECCCCCEEEEECCCCHHCCCEEEEEEEECCCEEEEEECCEEEEEECCCCCCCCCCC
LNAWDATAVFQHVYAKR
CCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKRMIQMGIIGAMMFPEAFSAAAADPDYYNEDHRPKYHFTPEANWMNDPNGMVYYAGEY
CCCCEEEHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEECEE
HLFYQYHPYGLRWGPMHWGHAVSKDLVKWEHLPVALYPDEKGTIFSGSAVADRHNTTGFQ
EEEEEECCCCEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEECCEECCCCCCCCCC
TGTEKPLVAIYTQDRSGEQVQSIAYSNDKGRTWTKYSGNPVIPNPGKRDFRDPKVIWHEQ
CCCCCCEEEEEECCCCCCHHHHHEECCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEECC
TKKWVMLLAGGDRILIYTSPDLKRWTYASEFGEGEGSHGGVWECPDLFELPVEGRPNETK
CCEEEEEEECCCEEEEEECCCCCCEEEHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCE
WVMQVSVGDGAVSGGSGMQYFVGSFDGTTFKNENPPNRVLWTDYGKDFYAAVSWSDIPKS
EEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCC
DGRRLWLGWMSNWQYANDVPTSPWRSAMSIPREVKLKAFSEGLRMIQAPVAELQSIRGAS
CCCEEEEEECCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
QTWKNKIISPRNGNLLKGLSGDAYEINAEFQVNTGAAAEFGFKVRTGENEYTKIGYSKNS
HHHHHCCCCCCCCCEEECCCCCEEEEEEEEEECCCCCEECCEEEECCCCCEEEEEECCCC
ASLFVDRSQSGNVSFNPNFNTGKHAAPLEPVAGKVKMRIYVDRSSVEVFGNDGRQVITDI
CEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEECCCCCEEEEEE
ILPDQSSKGLEVYASNGFVKLNSMTVHPLKKVWGASPFQSNLTGWTTVNGVWADTIDGKQ
ECCCCCCCCEEEEECCCEEEEECEEECHHHHHHCCCCCCCCCCCEEEECCEEEECCCCCC
GRSEGDSFILSSAKATDFTYEADVAIKDGNGRGAGALVFRADQDVKNGYLANVDAKHDVV
CCCCCCEEEEECCCCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCCCEEEECCCCCCEE
KFFKFEDGSASVIAEHRTPIETGRKYHLKTVARGANFYIYLDDQLVISARDSTFADGAFG
EEEEECCCCCEEEEECCCCHHCCCEEEEEEEECCCEEEEEECCEEEEEECCCCCCCCCCC
LNAWDATAVFQHVYAKR
CCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3112519; 3120151; 9141695; 9384377; 2117666 [H]