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The map label for this gene is gcvT

Identifier: 52786369

GI number: 52786369

Start: 2545979

End: 2547073

Strand: Reverse

Name: gcvT

Synonym: BLi02631

Alternate gene names: 52786369

Gene position: 2547073-2545979 (Counterclockwise)

Preceding gene: 52786370

Following gene: 52786368

Centisome position: 60.32

GC content: 50.5

Gene sequence:

>1095_bases
ATGCTTAAACGTACGCCTTTATTTGACCTTTATAAGGAATATGGCGGGAAAACGATTGATTTTGGCGGCTGGGAACTGCC
AGTCCAATTTTCTTCTATTAAAGAGGAGCACGAAGCCGTCAGGACGAAAGCGGGGCTTTTTGACGTCTCACACATGGGAG
AGGTCGAAATCACAGGAACGGACAGCCTTCCGTTCTTGCAAAAGCTGTTGACAAACGATGTTTCGACGCTGAAAGAAGGC
GGCGCCCAGTATACGGCTATGTGCTATGAAGACGGCGGAACGATCGACGATCTCCTCGTCTATAAAAAAGCGGCCAATGT
CTATATGCTCGTCATTAACGCAGCCAATATCGACAAGGACGTCGATTGGATGAACAAGCATATAAAGGGTGACGTGTCGG
TTCGAAACGTATCTGACGAGATTGCGCTCCTTGCCCTTCAAGGGCCGAAGGCCGAAGCAATTTTAAAACAGGTCGCAGAT
CATGACCTTGCAGAATTAAAGCCGTTTATGTTCCGGGATGATGCAGCGGTTGGCAGCGTGCAGGCGCTTGTGTCCAGAAC
GGGATACACGGGAGAGGACGGCTTTGAGATTTACTGCCGGAATGAAGATGCAGCCTGCATTTGGAAGCTGCTCCTTGAAA
CGGGCAAGGATTCGGGATTGGTGCCGTGCGGCCTCGGGGCTCGGGATACGCTGAGGTTTGAAGCGAAGCTGCCGCTTTAC
GGACAGGAGCTCTCAAAAGACATTACGCCGATCGAAGCCGGCATCGGATTTGCCGTCAAAACGAACAAAGCCTCGGATTT
CATCGGAAAAGCCGTCCTGGCAAGCCAAAAGGAACATGGAGCGGACAGAAAGCTGGTTGGCCTTGAAATGATCGATAAAG
GCATTCCAAGACACGGATACGCCGTCTATTATCAAGGCGAACAAGCAGGCGAGGTGACAACCGGCACACAGTCGCCGACT
TTAAAGAAAAACGTCGGCCTCGCATTGCTGAAAAAAGAAGCCTGCGCCCTCGACACCGTAGTTGAAGTGGAGATCAGAAA
TAAACGTTTGAAAGCCAAAATCGTGAAAACCCCATTTTACAAACGGCAGCCGTAA

Upstream 100 bases:

>100_bases
GTATAAAGCGGCGCAGCCATCCTGTTGATGTTGAAATCTTTCTTGTTTTCTGATGATTGGCAAACTTGTTTTTAGTTTGA
AAAGCGAAGGGGGAAGCAGA

Downstream 100 bases:

>100_bases
GTTTGCCAAGACCTTGAAAGGAGACTGAAGATGAAGCACCGTTACCTGCCAGCTACAGATCAGGATAAAAAGGAGATGCT
TGAGGCAATTGGAGTCGAGA

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MLKRTPLFDLYKEYGGKTIDFGGWELPVQFSSIKEEHEAVRTKAGLFDVSHMGEVEITGTDSLPFLQKLLTNDVSTLKEG
GAQYTAMCYEDGGTIDDLLVYKKAANVYMLVINAANIDKDVDWMNKHIKGDVSVRNVSDEIALLALQGPKAEAILKQVAD
HDLAELKPFMFRDDAAVGSVQALVSRTGYTGEDGFEIYCRNEDAACIWKLLLETGKDSGLVPCGLGARDTLRFEAKLPLY
GQELSKDITPIEAGIGFAVKTNKASDFIGKAVLASQKEHGADRKLVGLEMIDKGIPRHGYAVYYQGEQAGEVTTGTQSPT
LKKNVGLALLKKEACALDTVVEVEIRNKRLKAKIVKTPFYKRQP

Sequences:

>Translated_364_residues
MLKRTPLFDLYKEYGGKTIDFGGWELPVQFSSIKEEHEAVRTKAGLFDVSHMGEVEITGTDSLPFLQKLLTNDVSTLKEG
GAQYTAMCYEDGGTIDDLLVYKKAANVYMLVINAANIDKDVDWMNKHIKGDVSVRNVSDEIALLALQGPKAEAILKQVAD
HDLAELKPFMFRDDAAVGSVQALVSRTGYTGEDGFEIYCRNEDAACIWKLLLETGKDSGLVPCGLGARDTLRFEAKLPLY
GQELSKDITPIEAGIGFAVKTNKASDFIGKAVLASQKEHGADRKLVGLEMIDKGIPRHGYAVYYQGEQAGEVTTGTQSPT
LKKNVGLALLKKEACALDTVVEVEIRNKRLKAKIVKTPFYKRQP
>Mature_364_residues
MLKRTPLFDLYKEYGGKTIDFGGWELPVQFSSIKEEHEAVRTKAGLFDVSHMGEVEITGTDSLPFLQKLLTNDVSTLKEG
GAQYTAMCYEDGGTIDDLLVYKKAANVYMLVINAANIDKDVDWMNKHIKGDVSVRNVSDEIALLALQGPKAEAILKQVAD
HDLAELKPFMFRDDAAVGSVQALVSRTGYTGEDGFEIYCRNEDAACIWKLLLETGKDSGLVPCGLGARDTLRFEAKLPLY
GQELSKDITPIEAGIGFAVKTNKASDFIGKAVLASQKEHGADRKLVGLEMIDKGIPRHGYAVYYQGEQAGEVTTGTQSPT
LKKNVGLALLKKEACALDTVVEVEIRNKRLKAKIVKTPFYKRQP

Specific function: The glycine cleavage system catalyzes the degradation of glycine

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family

Homologues:

Organism=Homo sapiens, GI44662838, Length=367, Percent_Identity=35.149863760218, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI257796258, Length=353, Percent_Identity=34.8441926345609, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI257796254, Length=366, Percent_Identity=32.7868852459016, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI24797151, Length=340, Percent_Identity=28.5294117647059, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI257796256, Length=313, Percent_Identity=32.9073482428115, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI197927446, Length=334, Percent_Identity=26.9461077844311, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI21361378, Length=334, Percent_Identity=26.9461077844311, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI194306651, Length=385, Percent_Identity=24.4155844155844, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1789272, Length=371, Percent_Identity=39.622641509434, Blast_Score=260, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17560118, Length=374, Percent_Identity=36.096256684492, Blast_Score=191, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI71994045, Length=375, Percent_Identity=25.3333333333333, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71994052, Length=375, Percent_Identity=25.3333333333333, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32563613, Length=351, Percent_Identity=26.4957264957265, Blast_Score=111, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6320222, Length=385, Percent_Identity=32.7272727272727, Blast_Score=177, Evalue=2e-45,
Organism=Drosophila melanogaster, GI20129441, Length=375, Percent_Identity=35.2, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI28571104, Length=352, Percent_Identity=26.9886363636364, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI20130091, Length=365, Percent_Identity=24.9315068493151, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GCST_BACLD (Q65HF9)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_079784.1
- RefSeq:   YP_092198.1
- HSSP:   P54378
- ProteinModelPortal:   Q65HF9
- SMR:   Q65HF9
- STRING:   Q65HF9
- EnsemblBacteria:   EBBACT00000057364
- EnsemblBacteria:   EBBACT00000059689
- GeneID:   3028919
- GeneID:   3097439
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02631
- KEGG:   bli:BL01562
- NMPDR:   fig|279010.5.peg.476
- eggNOG:   COG0404
- GeneTree:   EBGT00050000001932
- HOGENOM:   HBG299834
- OMA:   LMTNDIN
- ProtClustDB:   PRK00389
- BioCyc:   BLIC279010-1:BLI02631-MONOMER
- BioCyc:   BLIC279010:BL01562-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00259
- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903
- PIRSF:   PIRSF006487
- TIGRFAMs:   TIGR00528

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: =2.1.2.10

Molecular weight: Translated: 40000; Mature: 40000

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRTPLFDLYKEYGGKTIDFGGWELPVQFSSIKEEHEAVRTKAGLFDVSHMGEVEITGT
CCCCCCHHHHHHHHCCCEEECCCEEEEEEHHHHHHHHHHHHHHHCEEECCCCCEEEEECC
DSLPFLQKLLTNDVSTLKEGGAQYTAMCYEDGGTIDDLLVYKKAANVYMLVINAANIDKD
CCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHCCCCEEEEEEEECCCCCC
VDWMNKHIKGDVSVRNVSDEIALLALQGPKAEAILKQVADHDLAELKPFMFRDDAAVGSV
HHHHHHHCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHCCCHHHCCCCEEECCCHHHHH
QALVSRTGYTGEDGFEIYCRNEDAACIWKLLLETGKDSGLVPCGLGARDTLRFEAKLPLY
HHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEECCCC
GQELSKDITPIEAGIGFAVKTNKASDFIGKAVLASQKEHGADRKLVGLEMIDKGIPRHGY
HHHHCCCCCCHHHCCCEEEECCCCHHHHHHHHHHCCHHCCCCCEEEEHHHHHCCCCCCCE
AVYYQGEQAGEVTTGTQSPTLKKNVGLALLKKEACALDTVVEVEIRNKRLKAKIVKTPFY
EEEECCCCCCCEECCCCCCCHHHCCCHHEEHHHHHHHCEEEEEEECCCEEEEEEECCCCC
KRQP
CCCC
>Mature Secondary Structure
MLKRTPLFDLYKEYGGKTIDFGGWELPVQFSSIKEEHEAVRTKAGLFDVSHMGEVEITGT
CCCCCCHHHHHHHHCCCEEECCCEEEEEEHHHHHHHHHHHHHHHCEEECCCCCEEEEECC
DSLPFLQKLLTNDVSTLKEGGAQYTAMCYEDGGTIDDLLVYKKAANVYMLVINAANIDKD
CCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHCCCCEEEEEEEECCCCCC
VDWMNKHIKGDVSVRNVSDEIALLALQGPKAEAILKQVADHDLAELKPFMFRDDAAVGSV
HHHHHHHCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHCCCHHHCCCCEEECCCHHHHH
QALVSRTGYTGEDGFEIYCRNEDAACIWKLLLETGKDSGLVPCGLGARDTLRFEAKLPLY
HHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEECCCC
GQELSKDITPIEAGIGFAVKTNKASDFIGKAVLASQKEHGADRKLVGLEMIDKGIPRHGY
HHHHCCCCCCHHHCCCEEEECCCCHHHHHHHHHHCCHHCCCCCEEEEHHHHHCCCCCCCE
AVYYQGEQAGEVTTGTQSPTLKKNVGLALLKKEACALDTVVEVEIRNKRLKAKIVKTPFY
EEEECCCCCCCEECCCCCCCHHHCCCHHEEHHHHHHHCEEEEEEECCCEEEEEEECCCCC
KRQP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA