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The map label for this gene is bkdB [H]

Identifier: 52786318

GI number: 52786318

Start: 2497848

End: 2499128

Strand: Reverse

Name: bkdB [H]

Synonym: BLi02580

Alternate gene names: 52786318

Gene position: 2499128-2497848 (Counterclockwise)

Preceding gene: 52786319

Following gene: 52786316

Centisome position: 59.18

GC content: 51.13

Gene sequence:

>1281_bases
ATGGCAGTGGAACAAATGACAATGCCGCAGCTCGGGGAGAGCGTGACTGAGGGAACCATCAGCAAATGGCTCGTCAGCGT
CGGAGATCACGTCAACAAATATGATCCGATTGCTGAAGTGATGACGGATAAGGTCAATGCTGAAGTCCCTTCATCGTTCA
CGGGAACGATTGCCGAGCTAGTCGGGAAGGAAGGCGAAACTCTGCAAGTCGGAGACGTCATCTGCAAAGTCGAAACAAAT
GAAGAAGCGAAACCGGAGGCTGAGGCTGTTTCCAAGCCAGATCAAGAGGAAGCGGAGCCGGCCAAACCGGAGGCGAAAGA
TACATCTCAGAAAAAACGTTATTCCCCGGCGGTGCTGAGACTTGCCGGAGAGCACAACATCGATTTGGAGCAGGTGGAAG
GTACAGGCGCCGGCGGACGGATCACAAGAAAAGACATCCAGCGCATCATTGCTTCCGGAGCTGTTCCTCAAACTGATGCA
GCGCCGGAAAAACAGCCGGGTGCAAATGCCGCGGGAGCGATTGAACCTGATCAAAAACCGGCGCAAGCCGCACCTCAAGC
TGCACCGCCTCAAAGTGCGGCCGGAGACGTTGAGATTCCTGTAACTGGAATCCGCAATGCGATTGCGACCAACATGGTCA
GAAGCAAGCATGAAATCCCTCATGCCTGGACGATGATGGAAGTGGATGTCACAGGTCTTGTGAGCTACCGCAACAAAATA
AAAAACGAATTCAAGAAAAAAGAAGGCTTCAGCCTGACCTTTTTCGCGTTTTTTGTCAAAGCAGTCGCACAGGCGCTGAA
GGAATTCCCGCAAATGAACAGCATGTGGGCCGGAGATAAAATCATTCAGAAAAAAGACATCAACATCTCAATTGCAGTAG
CGACTGAAGATGCGTTATATGTTCCTGTCATCAAGCATGCCGATGAAAAAACGATTAAAGGCATCGCAAGGGAAATCTCC
GAACTGGCTCATAAGGTCAGAAGCGGCAAGCTGACCAGCGGGGATATGAGCGGAGGTACGTTTACGGTCAATAATACCGG
TTCGTTTGGATCGGTTCAGTCGATGGGGATCATCAACCATCCGCAGGCTGCCATCCTGCAGGTTGAATCGATCGTCAAGC
GTCCCGTCGTCATGGAGCACGGCATGATCGCGGTCAGGGATATGGTGAACCTATGCTTATCCCTCGATCACAGAGTGCTT
GACGGCCTCATTTGCGGCAGATTCCTTGCCCGTGTGAAAGAGATTCTTGAACAGATCGATGATCATACGTCCATCTATTA
A

Upstream 100 bases:

>100_bases
CTCCTACAATGGAAAAATTCTTTATGGTCAATCCGGATAAAGCAGAAGCAGCGATGAGAGAATTGGCTGAGTTTTAATAT
AGCGAAGGAGGACACATCGT

Downstream 100 bases:

>100_bases
AAAAGCCGGCCCGTTTAGGGTCAGCTTTTTTCTTTTTCTAAGGGGGTGAGCATGCGGAGGTGCCCGTTTAGCACCTCGAT
TGAAGCCGGCGTTTTTGTGC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MAVEQMTMPQLGESVTEGTISKWLVSVGDHVNKYDPIAEVMTDKVNAEVPSSFTGTIAELVGKEGETLQVGDVICKVETN
EEAKPEAEAVSKPDQEEAEPAKPEAKDTSQKKRYSPAVLRLAGEHNIDLEQVEGTGAGGRITRKDIQRIIASGAVPQTDA
APEKQPGANAAGAIEPDQKPAQAAPQAAPPQSAAGDVEIPVTGIRNAIATNMVRSKHEIPHAWTMMEVDVTGLVSYRNKI
KNEFKKKEGFSLTFFAFFVKAVAQALKEFPQMNSMWAGDKIIQKKDINISIAVATEDALYVPVIKHADEKTIKGIAREIS
ELAHKVRSGKLTSGDMSGGTFTVNNTGSFGSVQSMGIINHPQAAILQVESIVKRPVVMEHGMIAVRDMVNLCLSLDHRVL
DGLICGRFLARVKEILEQIDDHTSIY

Sequences:

>Translated_426_residues
MAVEQMTMPQLGESVTEGTISKWLVSVGDHVNKYDPIAEVMTDKVNAEVPSSFTGTIAELVGKEGETLQVGDVICKVETN
EEAKPEAEAVSKPDQEEAEPAKPEAKDTSQKKRYSPAVLRLAGEHNIDLEQVEGTGAGGRITRKDIQRIIASGAVPQTDA
APEKQPGANAAGAIEPDQKPAQAAPQAAPPQSAAGDVEIPVTGIRNAIATNMVRSKHEIPHAWTMMEVDVTGLVSYRNKI
KNEFKKKEGFSLTFFAFFVKAVAQALKEFPQMNSMWAGDKIIQKKDINISIAVATEDALYVPVIKHADEKTIKGIAREIS
ELAHKVRSGKLTSGDMSGGTFTVNNTGSFGSVQSMGIINHPQAAILQVESIVKRPVVMEHGMIAVRDMVNLCLSLDHRVL
DGLICGRFLARVKEILEQIDDHTSIY
>Mature_425_residues
AVEQMTMPQLGESVTEGTISKWLVSVGDHVNKYDPIAEVMTDKVNAEVPSSFTGTIAELVGKEGETLQVGDVICKVETNE
EAKPEAEAVSKPDQEEAEPAKPEAKDTSQKKRYSPAVLRLAGEHNIDLEQVEGTGAGGRITRKDIQRIIASGAVPQTDAA
PEKQPGANAAGAIEPDQKPAQAAPQAAPPQSAAGDVEIPVTGIRNAIATNMVRSKHEIPHAWTMMEVDVTGLVSYRNKIK
NEFKKKEGFSLTFFAFFVKAVAQALKEFPQMNSMWAGDKIIQKKDINISIAVATEDALYVPVIKHADEKTIKGIAREISE
LAHKVRSGKLTSGDMSGGTFTVNNTGSFGSVQSMGIINHPQAAILQVESIVKRPVVMEHGMIAVRDMVNLCLSLDHRVLD
GLICGRFLARVKEILEQIDDHTSIY

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=418, Percent_Identity=31.3397129186603, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI203098753, Length=447, Percent_Identity=30.2013422818792, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI203098816, Length=447, Percent_Identity=30.2013422818792, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI31711992, Length=446, Percent_Identity=30.2690582959641, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI19923748, Length=247, Percent_Identity=35.2226720647773, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI260898739, Length=157, Percent_Identity=37.5796178343949, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786946, Length=419, Percent_Identity=33.1742243436754, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1786305, Length=436, Percent_Identity=30.5045871559633, Blast_Score=172, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=426, Percent_Identity=29.1079812206573, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI25146366, Length=411, Percent_Identity=31.1435523114355, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17560088, Length=453, Percent_Identity=29.1390728476821, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=29.2207792207792, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=421, Percent_Identity=32.7790973871734, Blast_Score=202, Evalue=9e-53,
Organism=Saccharomyces cerevisiae, GI6324258, Length=448, Percent_Identity=26.3392857142857, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI18859875, Length=434, Percent_Identity=29.9539170506912, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI20129315, Length=222, Percent_Identity=33.3333333333333, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24582497, Length=222, Percent_Identity=33.3333333333333, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24645909, Length=215, Percent_Identity=35.3488372093023, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 46084; Mature: 45953

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEQMTMPQLGESVTEGTISKWLVSVGDHVNKYDPIAEVMTDKVNAEVPSSFTGTIAEL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VGKEGETLQVGDVICKVETNEEAKPEAEAVSKPDQEEAEPAKPEAKDTSQKKRYSPAVLR
HCCCCCEEEECCEEEEEECCCCCCCHHHHHCCCCHHCCCCCCCCCCCCHHHHHCCCHHEE
LAGEHNIDLEQVEGTGAGGRITRKDIQRIIASGAVPQTDAAPEKQPGANAAGAIEPDQKP
ECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AQAAPQAAPPQSAAGDVEIPVTGIRNAIATNMVRSKHEIPHAWTMMEVDVTGLVSYRNKI
HHCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHH
KNEFKKKEGFSLTFFAFFVKAVAQALKEFPQMNSMWAGDKIIQKKDINISIAVATEDALY
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHCCCCCCCHHEEECCCEEEEEEECCCCEE
VPVIKHADEKTIKGIAREISELAHKVRSGKLTSGDMSGGTFTVNNTGSFGSVQSMGIINH
EHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHCCCCCCC
PQAAILQVESIVKRPVVMEHGMIAVRDMVNLCLSLDHRVLDGLICGRFLARVKEILEQID
CHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHTSIY
HHCCCC
>Mature Secondary Structure 
AVEQMTMPQLGESVTEGTISKWLVSVGDHVNKYDPIAEVMTDKVNAEVPSSFTGTIAEL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VGKEGETLQVGDVICKVETNEEAKPEAEAVSKPDQEEAEPAKPEAKDTSQKKRYSPAVLR
HCCCCCEEEECCEEEEEECCCCCCCHHHHHCCCCHHCCCCCCCCCCCCHHHHHCCCHHEE
LAGEHNIDLEQVEGTGAGGRITRKDIQRIIASGAVPQTDAAPEKQPGANAAGAIEPDQKP
ECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AQAAPQAAPPQSAAGDVEIPVTGIRNAIATNMVRSKHEIPHAWTMMEVDVTGLVSYRNKI
HHCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHH
KNEFKKKEGFSLTFFAFFVKAVAQALKEFPQMNSMWAGDKIIQKKDINISIAVATEDALY
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHCCCCCCCHHEEECCCEEEEEEECCCCEE
VPVIKHADEKTIKGIAREISELAHKVRSGKLTSGDMSGGTFTVNNTGSFGSVQSMGIINH
EHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHCCCCCCC
PQAAILQVESIVKRPVVMEHGMIAVRDMVNLCLSLDHRVLDGLICGRFLARVKEILEQID
CHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHTSIY
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]