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The map label for this gene is rluB [H]

Identifier: 52786201

GI number: 52786201

Start: 2398878

End: 2399612

Strand: Reverse

Name: rluB [H]

Synonym: BLi02462

Alternate gene names: 52786201

Gene position: 2399612-2398878 (Counterclockwise)

Preceding gene: 52786202

Following gene: 52786200

Centisome position: 56.83

GC content: 47.48

Gene sequence:

>735_bases
ATGGAACGACTTCAAAAAGTAATCGCCCACGCCGGTATCGCATCGAGGCGGAAGGCGGAGGAGCTTATAAAAGAAGGGAG
AGTCACGGTCAACGGCAAAACCGTCAGAGAGCTTGGCGTTAAAGTATCAAGCTCCGATCGGATTGAGGTAAACGGCGTCC
AGCTTGAAAGCGAAGAACCCGTTTATTTTCTCCTGTATAAACCGAGAGGGGTTATATCAGCAGTTAAAGACGACAAAGGC
CGGAAAGTTGTGACCGACTTTTTCACCGAGGTGGAGGAGCGGATTTATCCGATCGGAAGGCTTGATTATGATACGAGCGG
CCTCTTATTGCTAACGAATGACGGGGACTTCGCCAATAAATTGATGCACCCGAAATACGAAATTAATAAAACGTATGTAG
CAAAGCTGAAAGGCATTCCTTTAAAGGAGAATTTAAAAAAACTTGAGCGGGGCGTGCGCCTTGAGGATGGAAAGACAGCT
CCTGCAAAAGTGAAGCTTTTGTCGCTTGATAAGAAAAAGCAGACAAGCATCGTCCAAATCACGATCCATGAGGGGCGAAA
CCGCCAGGTCAGGAGAATGTTTGAAGCCATCGGACACGAGGTGCTCAAGCTGAAAAGGGAAGAGTATGCTTTTTTGAACC
TGGCGGGCTTGAAAACAGGGGAAAAAAGAGAGCTTACGCCTCATGAAGTCAAAAGGCTCAGGGCATTGGCAGAACACGGT
AAAGCGGCTTTCTAA

Upstream 100 bases:

>100_bases
TACATTTAAGGGCGGAGAGCTACTTTCCTAAGGAAGAAGGCGCCTCGCAAACTTGTTTGTTGGTCTGGAAAAAAGTAAGA
TAATACATGAGGTGAAAGAT

Downstream 100 bases:

>100_bases
ATTTTCACACAACCTTCAAAAAAGGCAAATGGCAGTCAGTCTTGAGATGATATAATGATACTGAATGTCTACATGAGGGG
GCGGATTATGAAAAAAAAGC

Product: RluB

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MERLQKVIAHAGIASRRKAEELIKEGRVTVNGKTVRELGVKVSSSDRIEVNGVQLESEEPVYFLLYKPRGVISAVKDDKG
RKVVTDFFTEVEERIYPIGRLDYDTSGLLLLTNDGDFANKLMHPKYEINKTYVAKLKGIPLKENLKKLERGVRLEDGKTA
PAKVKLLSLDKKKQTSIVQITIHEGRNRQVRRMFEAIGHEVLKLKREEYAFLNLAGLKTGEKRELTPHEVKRLRALAEHG
KAAF

Sequences:

>Translated_244_residues
MERLQKVIAHAGIASRRKAEELIKEGRVTVNGKTVRELGVKVSSSDRIEVNGVQLESEEPVYFLLYKPRGVISAVKDDKG
RKVVTDFFTEVEERIYPIGRLDYDTSGLLLLTNDGDFANKLMHPKYEINKTYVAKLKGIPLKENLKKLERGVRLEDGKTA
PAKVKLLSLDKKKQTSIVQITIHEGRNRQVRRMFEAIGHEVLKLKREEYAFLNLAGLKTGEKRELTPHEVKRLRALAEHG
KAAF
>Mature_244_residues
MERLQKVIAHAGIASRRKAEELIKEGRVTVNGKTVRELGVKVSSSDRIEVNGVQLESEEPVYFLLYKPRGVISAVKDDKG
RKVVTDFFTEVEERIYPIGRLDYDTSGLLLLTNDGDFANKLMHPKYEINKTYVAKLKGIPLKENLKKLERGVRLEDGKTA
PAKVKLLSLDKKKQTSIVQITIHEGRNRQVRRMFEAIGHEVLKLKREEYAFLNLAGLKTGEKRELTPHEVKRLRALAEHG
KAAF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=246, Percent_Identity=39.8373983739837, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1790453, Length=243, Percent_Identity=35.3909465020576, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1788510, Length=233, Percent_Identity=35.1931330472103, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI87081838, Length=182, Percent_Identity=36.8131868131868, Blast_Score=110, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27743; Mature: 27743

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLQKVIAHAGIASRRKAEELIKEGRVTVNGKTVRELGVKVSSSDRIEVNGVQLESEEP
CHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCCHHHHCCEECCCCEEEEEEEEECCCCC
VYFLLYKPRGVISAVKDDKGRKVVTDFFTEVEERIYPIGRLDYDTSGLLLLTNDGDFANK
EEEEEECCCCCEEHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHH
LMHPKYEINKTYVAKLKGIPLKENLKKLERGVRLEDGKTAPAKVKLLSLDKKKQTSIVQI
HCCCCCCCCCEEEEHCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCEEEEE
TIHEGRNRQVRRMFEAIGHEVLKLKREEYAFLNLAGLKTGEKRELTPHEVKRLRALAEHG
EEECCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHCC
KAAF
CCCC
>Mature Secondary Structure
MERLQKVIAHAGIASRRKAEELIKEGRVTVNGKTVRELGVKVSSSDRIEVNGVQLESEEP
CHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCCHHHHCCEECCCCEEEEEEEEECCCCC
VYFLLYKPRGVISAVKDDKGRKVVTDFFTEVEERIYPIGRLDYDTSGLLLLTNDGDFANK
EEEEEECCCCCEEHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHH
LMHPKYEINKTYVAKLKGIPLKENLKKLERGVRLEDGKTAPAKVKLLSLDKKKQTSIVQI
HCCCCCCCCCEEEEHCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCEEEEE
TIHEGRNRQVRRMFEAIGHEVLKLKREEYAFLNLAGLKTGEKRELTPHEVKRLRALAEHG
EEECCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHCC
KAAF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]