Click here to switch to the map view.

The map label for this gene is gudB [H]

Identifier: 52786177

GI number: 52786177

Start: 2373180

End: 2374454

Strand: Reverse

Name: gudB [H]

Synonym: BLi02435

Alternate gene names: 52786177

Gene position: 2374454-2373180 (Counterclockwise)

Preceding gene: 52786178

Following gene: 52786176

Centisome position: 56.23

GC content: 48.47

Gene sequence:

>1275_bases
ATGGTAGCCGATCAAAACACCGGTCATACTGAAGAAAACACACATGACGTTTTGAAATCAACGCAAACGGTGATACATAA
GGCGCTTGAAAAATTGGGATATCCTGAAGAGGTGTATGAATTACTAAAAGAACCGATTCGTTTTTTAACGGTGAAAATTC
CGGTTCGCATGGACGATGGATCTGTTAAGATTTTCACGGGCTACCGAGCCCAGCACAATGATGCAGTAGGGCCGACAAAA
GGCGGCATCCGTTTTCATCCGGGAGTTACAGAAAAAGAAGTTAAAGCGCTTTCTATTTGGATGAGTCTGAAATGCGGCAT
CATCGACCTTCCATATGGAGGCGGAAAGGGCGGGATCATCTGTGATCCGCGGGATATGTCCTTTCCTGAACTGGAACGTT
TAAGCAGAGGGTACGTTCGGGCGATCAGCCAAATTGTCGGACCGACAAAAGACGTACCGGCTCCCGATGTATTTACCAAT
TCTCAAATCATGGCATGGATGATGGACGAATATTCAAGAATCGACGAATTCAACTCGCCGGGCTTCATTACCGGCAAACC
GCTCGTTTTAGGTGGTTCTCACGGCAGAGAATCTGCGACAGCAAAAGGGGTTACAATCTGCATTAAAGAGGCGGCGAAAA
AGAAAAACATCGACATTGAGGGAGCTTCTGTCGTCGTGCAGGGGTTCGGAAATGCGGGAAGCTACCTTGCGAAATTCATG
TATGACGCAGGTGCAAAGGTTGTCGGCATTTCTGATGCATACGGCGGCCTGTACGATCCTGAAGGGCTTGACATCGATTA
CCTGCTTGACCGCCGAGACAGCTTCGGAACGGTCACAAAGCTGTTTAATGACACCATTACAAACAAAGAACTGCTGGAGC
TGGAGTGCGACATTCTTGTGCCGGCTGCCATAGAAAATCAAATTACCGCGGAAAACGCCCATAACATCAAGGCGAAAATC
GTTGTCGAAGCTGCCAACGGCCCGACGACGCTCGAAGCGACGAAAATTCTTTCCGACCGCGACATTCTTCTTGTACCGGA
CGTGCTGGCAAGCGCCGGCGGTGTAACGGTATCCTATTTTGAGTGGGTGCAAAACAACCAGGGATTCTACTGGACTGAAG
AAGAAGTTGAAGAACGTTTGGAAAAAATGATGGTCAAATCGTTCAACAACATTTATGAAATGGCGCACAACCGCAGAATT
GACATGAGGCTTGCAGCCTATATGGTGGGCGTCCGCAAGATGGCGGAAGCATCCCGCTTCAGAGGCTGGATATAG

Upstream 100 bases:

>100_bases
ACGAAAAATGGTTGATTGTTCTTTGCATGATTAGTGCAAGGGTGTATACTATTCTCTGAAAGGACAACATGCCAGAAAGA
CTTTAGGAGGTTAACTTATA

Downstream 100 bases:

>100_bases
GCTCAAACATTTGCAACAACCCAAAAAATCTCCTATCATAAAATAGGAGATTTTTTGATTTGCCGGATATGACAGGAGAT
GAACGGAACATGAAAGAAGA

Product: GudB

Products: NA

Alternate protein names: NAD-GDH [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MVADQNTGHTEENTHDVLKSTQTVIHKALEKLGYPEEVYELLKEPIRFLTVKIPVRMDDGSVKIFTGYRAQHNDAVGPTK
GGIRFHPGVTEKEVKALSIWMSLKCGIIDLPYGGGKGGIICDPRDMSFPELERLSRGYVRAISQIVGPTKDVPAPDVFTN
SQIMAWMMDEYSRIDEFNSPGFITGKPLVLGGSHGRESATAKGVTICIKEAAKKKNIDIEGASVVVQGFGNAGSYLAKFM
YDAGAKVVGISDAYGGLYDPEGLDIDYLLDRRDSFGTVTKLFNDTITNKELLELECDILVPAAIENQITAENAHNIKAKI
VVEAANGPTTLEATKILSDRDILLVPDVLASAGGVTVSYFEWVQNNQGFYWTEEEVEERLEKMMVKSFNNIYEMAHNRRI
DMRLAAYMVGVRKMAEASRFRGWI

Sequences:

>Translated_424_residues
MVADQNTGHTEENTHDVLKSTQTVIHKALEKLGYPEEVYELLKEPIRFLTVKIPVRMDDGSVKIFTGYRAQHNDAVGPTK
GGIRFHPGVTEKEVKALSIWMSLKCGIIDLPYGGGKGGIICDPRDMSFPELERLSRGYVRAISQIVGPTKDVPAPDVFTN
SQIMAWMMDEYSRIDEFNSPGFITGKPLVLGGSHGRESATAKGVTICIKEAAKKKNIDIEGASVVVQGFGNAGSYLAKFM
YDAGAKVVGISDAYGGLYDPEGLDIDYLLDRRDSFGTVTKLFNDTITNKELLELECDILVPAAIENQITAENAHNIKAKI
VVEAANGPTTLEATKILSDRDILLVPDVLASAGGVTVSYFEWVQNNQGFYWTEEEVEERLEKMMVKSFNNIYEMAHNRRI
DMRLAAYMVGVRKMAEASRFRGWI
>Mature_424_residues
MVADQNTGHTEENTHDVLKSTQTVIHKALEKLGYPEEVYELLKEPIRFLTVKIPVRMDDGSVKIFTGYRAQHNDAVGPTK
GGIRFHPGVTEKEVKALSIWMSLKCGIIDLPYGGGKGGIICDPRDMSFPELERLSRGYVRAISQIVGPTKDVPAPDVFTN
SQIMAWMMDEYSRIDEFNSPGFITGKPLVLGGSHGRESATAKGVTICIKEAAKKKNIDIEGASVVVQGFGNAGSYLAKFM
YDAGAKVVGISDAYGGLYDPEGLDIDYLLDRRDSFGTVTKLFNDTITNKELLELECDILVPAAIENQITAENAHNIKAKI
VVEAANGPTTLEATKILSDRDILLVPDVLASAGGVTVSYFEWVQNNQGFYWTEEEVEERLEKMMVKSFNNIYEMAHNRRI
DMRLAAYMVGVRKMAEASRFRGWI

Specific function: The wild-type version has little or no activity [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=341, Percent_Identity=40.1759530791789, Blast_Score=265, Evalue=5e-71,
Organism=Homo sapiens, GI4885281, Length=341, Percent_Identity=39.8826979472141, Blast_Score=263, Evalue=3e-70,
Organism=Escherichia coli, GI1788059, Length=402, Percent_Identity=30.8457711442786, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17544676, Length=338, Percent_Identity=41.1242603550296, Blast_Score=256, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6324951, Length=408, Percent_Identity=28.6764705882353, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319256, Length=400, Percent_Identity=29, Blast_Score=144, Evalue=4e-35,
Organism=Drosophila melanogaster, GI45549226, Length=340, Percent_Identity=42.3529411764706, Blast_Score=275, Evalue=3e-74,
Organism=Drosophila melanogaster, GI45553475, Length=371, Percent_Identity=40.4312668463612, Blast_Score=275, Evalue=3e-74,
Organism=Drosophila melanogaster, GI24649283, Length=407, Percent_Identity=37.1007371007371, Blast_Score=258, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24649460, Length=279, Percent_Identity=41.5770609318996, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.2 [H]

Molecular weight: Translated: 46930; Mature: 46930

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVADQNTGHTEENTHDVLKSTQTVIHKALEKLGYPEEVYELLKEPIRFLTVKIPVRMDDG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEEEEECCC
SVKIFTGYRAQHNDAVGPTKGGIRFHPGVTEKEVKALSIWMSLKCGIIDLPYGGGKGGII
CEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHEEEEEECCEEECCCCCCCCCEE
CDPRDMSFPELERLSRGYVRAISQIVGPTKDVPAPDVFTNSQIMAWMMDEYSRIDEFNSP
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
GFITGKPLVLGGSHGRESATAKGVTICIKEAAKKKNIDIEGASVVVQGFGNAGSYLAKFM
CEECCCCEEEECCCCCCCCCCCCCEEEEHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHH
YDAGAKVVGISDAYGGLYDPEGLDIDYLLDRRDSFGTVTKLFNDTITNKELLELECDILV
HHCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECEEE
PAAIENQITAENAHNIKAKIVVEAANGPTTLEATKILSDRDILLVPDVLASAGGVTVSYF
CHHHCCCCCCCCCCCEEEEEEEEECCCCCEEHHHHHCCCCCEEEECHHHHCCCCCEEEEH
EWVQNNQGFYWTEEEVEERLEKMMVKSFNNIYEMAHNRRIDMRLAAYMVGVRKMAEASRF
HHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RGWI
CCCC
>Mature Secondary Structure
MVADQNTGHTEENTHDVLKSTQTVIHKALEKLGYPEEVYELLKEPIRFLTVKIPVRMDDG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEEEEECCC
SVKIFTGYRAQHNDAVGPTKGGIRFHPGVTEKEVKALSIWMSLKCGIIDLPYGGGKGGII
CEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHEEEEEECCEEECCCCCCCCCEE
CDPRDMSFPELERLSRGYVRAISQIVGPTKDVPAPDVFTNSQIMAWMMDEYSRIDEFNSP
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
GFITGKPLVLGGSHGRESATAKGVTICIKEAAKKKNIDIEGASVVVQGFGNAGSYLAKFM
CEECCCCEEEECCCCCCCCCCCCCEEEEHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHH
YDAGAKVVGISDAYGGLYDPEGLDIDYLLDRRDSFGTVTKLFNDTITNKELLELECDILV
HHCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECEEE
PAAIENQITAENAHNIKAKIVVEAANGPTTLEATKILSDRDILLVPDVLASAGGVTVSYF
CHHHCCCCCCCCCCCEEEEEEEEECCCCCEEHHHHHCCCCCEEEECHHHHCCCCCEEEEH
EWVQNNQGFYWTEEEVEERLEKMMVKSFNNIYEMAHNRRIDMRLAAYMVGVRKMAEASRF
HHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RGWI
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377; 9829940 [H]