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The map label for this gene is gpsA

Identifier: 52786162

GI number: 52786162

Start: 2359845

End: 2360885

Strand: Reverse

Name: gpsA

Synonym: BLi02420

Alternate gene names: 52786162

Gene position: 2360885-2359845 (Counterclockwise)

Preceding gene: 52786163

Following gene: 52786161

Centisome position: 55.91

GC content: 48.61

Gene sequence:

>1041_bases
ATGACAAAAGTATCGATTTTGGGAGCCGGAAGCTGGGGAACAGCCTTGGCGCTCGTTTTGGCGGACAACCAGTACGATGT
GTGCATATGGGGCCATCGTGAAGAACTGATTCAGCAAATCAATGAACATCATGAAAACAAAGATTATCTGCCGGGTGTCA
AACTTTCTGAAAACGTGCGTGCCACAACAGATCTTGAAGCTGCAGTGGCTGATGTCAAGACGATCATTGTCGCCGTTCCG
ACAAAAGCGATCCGCGAAGTTTTGGCACAGGCTGTTTCCTTCATCAACCAAAAGGCGATTTTTGTACATGTTTCAAAAGG
AATTGAGCCTGACACACTGCTTCGAATTTCTGAAATGATGAAAGAAGAAATTCCGGCTGAATTAAGGGAGGACATCGTCG
TTCTGTCCGGACCGAGCCATGCGGAAGAAGTCGGTTTAAGGCATCCGACGACAGTAACGGTATCTGCCGACAGCAGCATT
GAATCGGCCCAGGCGATCCAGGATATGTTTATGAACCAGAATTTCCGCGTGTATACGAATCCGGATATGATCGGCGTAGA
GATCGGCGGCGCGCTGAAAAACGTCATTGCGCTTGCCGCGGGCATTACAGACGGTTTGGGATACGGAGATAATGCGAAAG
CCGCGCTGATTACAAGGGGCTTGGCAGAAATCGCCCGGCTCGGCACAAAAATGGGCGGAAATCCGCTGACATTCTCGGGT
CTTACTGGAATCGGGGACTTGATCGTCACCTGCACAAGCGTCCATTCACGAAATTGGCGAGCCGGTAATATGCTGGGTAA
AGGCTTCAAGCTGGATGAAGTTCTGGAAGAAATGGGGATGGTCGTCGAGGGGGTCCGCACGACAAAAGCCGCTTATCAGC
TTTCAAAGAAATTTGATGTAAAAATGCCGATCACCGAAGCACTGCACAAAGTATTGTTTGACGGCGAAAAAGTTGAGACG
GCAGTCGAATCATTGATGGCAAGAGTCAAAACACACGAGATGGAAGATCTTGTGAACACATTCGAAAATCAAATGAAATG
A

Upstream 100 bases:

>100_bases
CGAACGCTTTTTGGAAAACCGGATACGCGACGCCTTCGGATTTGAAGGAACACCAATCAGGATTTTCGCAAGAGCAAGAA
AATAAAGAGGTGTAGTAAAA

Downstream 100 bases:

>100_bases
CCGCCCCATCATCAACTGCTTTTATACTCTCCTCTAAGTTCGTTTTAAAAAAACCAAAACATTTTGGAGACTTAGGCAAG
AGAGGATACATTTGTCCGAA

Product: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MTKVSILGAGSWGTALALVLADNQYDVCIWGHREELIQQINEHHENKDYLPGVKLSENVRATTDLEAAVADVKTIIVAVP
TKAIREVLAQAVSFINQKAIFVHVSKGIEPDTLLRISEMMKEEIPAELREDIVVLSGPSHAEEVGLRHPTTVTVSADSSI
ESAQAIQDMFMNQNFRVYTNPDMIGVEIGGALKNVIALAAGITDGLGYGDNAKAALITRGLAEIARLGTKMGGNPLTFSG
LTGIGDLIVTCTSVHSRNWRAGNMLGKGFKLDEVLEEMGMVVEGVRTTKAAYQLSKKFDVKMPITEALHKVLFDGEKVET
AVESLMARVKTHEMEDLVNTFENQMK

Sequences:

>Translated_346_residues
MTKVSILGAGSWGTALALVLADNQYDVCIWGHREELIQQINEHHENKDYLPGVKLSENVRATTDLEAAVADVKTIIVAVP
TKAIREVLAQAVSFINQKAIFVHVSKGIEPDTLLRISEMMKEEIPAELREDIVVLSGPSHAEEVGLRHPTTVTVSADSSI
ESAQAIQDMFMNQNFRVYTNPDMIGVEIGGALKNVIALAAGITDGLGYGDNAKAALITRGLAEIARLGTKMGGNPLTFSG
LTGIGDLIVTCTSVHSRNWRAGNMLGKGFKLDEVLEEMGMVVEGVRTTKAAYQLSKKFDVKMPITEALHKVLFDGEKVET
AVESLMARVKTHEMEDLVNTFENQMK
>Mature_345_residues
TKVSILGAGSWGTALALVLADNQYDVCIWGHREELIQQINEHHENKDYLPGVKLSENVRATTDLEAAVADVKTIIVAVPT
KAIREVLAQAVSFINQKAIFVHVSKGIEPDTLLRISEMMKEEIPAELREDIVVLSGPSHAEEVGLRHPTTVTVSADSSIE
SAQAIQDMFMNQNFRVYTNPDMIGVEIGGALKNVIALAAGITDGLGYGDNAKAALITRGLAEIARLGTKMGGNPLTFSGL
TGIGDLIVTCTSVHSRNWRAGNMLGKGFKLDEVLEEMGMVVEGVRTTKAAYQLSKKFDVKMPITEALHKVLFDGEKVETA
VESLMARVKTHEMEDLVNTFENQMK

Specific function: De novo phospholipid biosynthesis; glycerol-3 phosphate formation. [C]

COG id: COG0240

COG function: function code C; Glycerol-3-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI24307999, Length=347, Percent_Identity=31.700288184438, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI33695088, Length=345, Percent_Identity=32.463768115942, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI1790037, Length=331, Percent_Identity=40.1812688821752, Blast_Score=270, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI32564399, Length=330, Percent_Identity=33.3333333333333, Blast_Score=150, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI32564403, Length=338, Percent_Identity=33.7278106508876, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI193210136, Length=338, Percent_Identity=33.7278106508876, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17507425, Length=360, Percent_Identity=28.6111111111111, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI193210134, Length=330, Percent_Identity=30.6060606060606, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320181, Length=341, Percent_Identity=28.4457478005865, Blast_Score=125, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6324513, Length=350, Percent_Identity=26.2857142857143, Blast_Score=119, Evalue=5e-28,
Organism=Drosophila melanogaster, GI17136202, Length=357, Percent_Identity=31.6526610644258, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17136200, Length=332, Percent_Identity=32.8313253012048, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17136204, Length=332, Percent_Identity=32.8313253012048, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI22026922, Length=349, Percent_Identity=26.0744985673352, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI45551945, Length=236, Percent_Identity=30.5084745762712, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281362270, Length=236, Percent_Identity=30.5084745762712, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648969, Length=273, Percent_Identity=27.8388278388278, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPDA_BACLD (Q65I16)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_079583.1
- RefSeq:   YP_091991.1
- HSSP:   O29390
- ProteinModelPortal:   Q65I16
- SMR:   Q65I16
- STRING:   Q65I16
- EnsemblBacteria:   EBBACT00000057048
- EnsemblBacteria:   EBBACT00000060041
- GeneID:   3029133
- GeneID:   3099109
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02420
- KEGG:   bli:BL02791
- NMPDR:   fig|279010.5.peg.269
- eggNOG:   COG0240
- GeneTree:   EBGT00050000002224
- HOGENOM:   HBG586392
- OMA:   NVAKGIE
- ProtClustDB:   PRK00094
- BioCyc:   BLIC279010-1:BLI02420-MONOMER
- BioCyc:   BLIC279010:BL02791-MONOMER
- HAMAP:   MF_00394
- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR006168
- InterPro:   IPR006109
- InterPro:   IPR011128
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR11728
- PIRSF:   PIRSF000114
- PRINTS:   PR00077

Pfam domain/function: PF07479 NAD_Gly3P_dh_C; PF01210 NAD_Gly3P_dh_N; SSF48179 6DGDH_C_like

EC number: =1.1.1.94

Molecular weight: Translated: 37630; Mature: 37499

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00957 NAD_G3PDH

Important sites: ACT_SITE 193-193 BINDING 106-106 BINDING 106-106 BINDING 141-141 BINDING 257-257 BINDING 283-283

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVSILGAGSWGTALALVLADNQYDVCIWGHREELIQQINEHHENKDYLPGVKLSENVR
CCEEEEEECCCHHHEEEEEEECCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
ATTDLEAAVADVKTIIVAVPTKAIREVLAQAVSFINQKAIFVHVSKGIEPDTLLRISEMM
CHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHH
KEEIPAELREDIVVLSGPSHAEEVGLRHPTTVTVSADSSIESAQAIQDMFMNQNFRVYTN
HHHCCHHHHCCEEEEECCCCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEC
PDMIGVEIGGALKNVIALAAGITDGLGYGDNAKAALITRGLAEIARLGTKMGGNPLTFSG
CCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECC
LTGIGDLIVTCTSVHSRNWRAGNMLGKGFKLDEVLEEMGMVVEGVRTTKAAYQLSKKFDV
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KMPITEALHKVLFDGEKVETAVESLMARVKTHEMEDLVNTFENQMK
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKVSILGAGSWGTALALVLADNQYDVCIWGHREELIQQINEHHENKDYLPGVKLSENVR
CEEEEEECCCHHHEEEEEEECCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
ATTDLEAAVADVKTIIVAVPTKAIREVLAQAVSFINQKAIFVHVSKGIEPDTLLRISEMM
CHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHH
KEEIPAELREDIVVLSGPSHAEEVGLRHPTTVTVSADSSIESAQAIQDMFMNQNFRVYTN
HHHCCHHHHCCEEEEECCCCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEC
PDMIGVEIGGALKNVIALAAGITDGLGYGDNAKAALITRGLAEIARLGTKMGGNPLTFSG
CCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECC
LTGIGDLIVTCTSVHSRNWRAGNMLGKGFKLDEVLEEMGMVVEGVRTTKAAYQLSKKFDV
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KMPITEALHKVLFDGEKVETAVESLMARVKTHEMEDLVNTFENQMK
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA