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The map label for this gene is aroF [H]

Identifier: 52786150

GI number: 52786150

Start: 2349831

End: 2351003

Strand: Reverse

Name: aroF [H]

Synonym: BLi02406

Alternate gene names: 52786150

Gene position: 2351003-2349831 (Counterclockwise)

Preceding gene: 52786151

Following gene: 52786149

Centisome position: 55.68

GC content: 51.66

Gene sequence:

>1173_bases
ATGAGGTATTTAACAGCCGGTGAATCTCACGGTCCGCAGCTTACGACAATTATCGAAGGAGTCCCAGCGGGGCTTTATAT
CACAAAAGAAGACATCGATTACGAGCTTCAAAGACGTCAAAAAGGCCACGGCCGCGGACGCCGGATGCAAATCGAAAAAG
ACCAGGCGCGGATCACAAGCGGTGTAAGACACGGGCGTTCTCTTGGATCGCCGATCGCTCTCGTCGTCGAAAACAATGAC
TGGAAACACTGGACGAAAATCATGGGTTCAGAGCCGATCACGGCTGAAGAAGAAAGCGAAATGAAACGACAGATTTCAAG
GCCGAGACCGGGGCACGCCGATTTGAACGGCGCGATCAAATACGGGCACAGAGACATGCGCAACGTGCTTGAACGTTCAT
CTGCCAGAGAAACGACCGTCCGTGTAGCGGCAGGAGCCGTCGCCAAAAAGATCCTTTCCCAGCTCGGCATTCAAGTGGCC
GGCCACGTGCTGGAGATCGGCGGAGTAAAAGCTGAAAAAACAAATTACGAATCTATCGAAAACTTGAAAAAAGTAACCGA
AGAGTCCCCTGTCAGATGCTATGACGAAGAAGCGGGCAGGAAAATGATGGACGCGATCGATGAAGCAAAAGCAAATGGGG
ATTCGATCGGCGGAATCGTCGAGGTGATAGTTGAAGGGGTGCCGGTAGGAGTCGGCAGCTATGTCCATTACGACCGAAAA
CTCGACAGCAAGCTTGCAGCCGCCGTACTGAGCATCAATGCTTTTAAAGGGGTTGAATTCGGCATCGGCTTTGAAGCCGC
ACGCAAAAACGGCAGTGAAGTCCATGACGAAATCATCTGGGACGAAGAAAAAGGCTATACGCGGGCAACAAACCGTCTCG
GCGGTCTTGAAGGCGGAATGACAACGGGAATGCCGATTGTCGTCAGAGGTGTGATGAAGCCGATTCCAACGTTATACAAA
CCGCTTAAAAGCGTTGATATCGAGACAAAAGAGCCGTTTACAGCGAGCATCGAGCGTTCCGACTCCTGCGCTGTCCCTGC
CGCAAGCGTGGTGGCTGAAGCGGTTGTTGCATGGGAAATCGCAAATGCGATCGTCGAGCAGTTTGGCGTTGACCAAATGG
ACCGGATCGCTGAAAATGTTGAGAAAATGAGAAAGCTTGCGAGGGAGTTTTAA

Upstream 100 bases:

>100_bases
CAGATACGTTTTTCTATCAAAAAAGTTAGCTGACTTTTTATAAAGGGTTATGTTATAGTTTTACTTAAAAGCGTTAATGT
GATGAAGGGAGAACGAAAGC

Downstream 100 bases:

>100_bases
TGAAATCACTTCAAATCGAAACCGCTTCTTTGTCCTACCCGGTATTCATCGGGGGAGGCATTAGAAAAAACGCGGCAGAG
CTTTTGTCATCCTTGAACCT

Product: chorismate synthase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate phospholyase [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MRYLTAGESHGPQLTTIIEGVPAGLYITKEDIDYELQRRQKGHGRGRRMQIEKDQARITSGVRHGRSLGSPIALVVENND
WKHWTKIMGSEPITAEEESEMKRQISRPRPGHADLNGAIKYGHRDMRNVLERSSARETTVRVAAGAVAKKILSQLGIQVA
GHVLEIGGVKAEKTNYESIENLKKVTEESPVRCYDEEAGRKMMDAIDEAKANGDSIGGIVEVIVEGVPVGVGSYVHYDRK
LDSKLAAAVLSINAFKGVEFGIGFEAARKNGSEVHDEIIWDEEKGYTRATNRLGGLEGGMTTGMPIVVRGVMKPIPTLYK
PLKSVDIETKEPFTASIERSDSCAVPAASVVAEAVVAWEIANAIVEQFGVDQMDRIAENVEKMRKLAREF

Sequences:

>Translated_390_residues
MRYLTAGESHGPQLTTIIEGVPAGLYITKEDIDYELQRRQKGHGRGRRMQIEKDQARITSGVRHGRSLGSPIALVVENND
WKHWTKIMGSEPITAEEESEMKRQISRPRPGHADLNGAIKYGHRDMRNVLERSSARETTVRVAAGAVAKKILSQLGIQVA
GHVLEIGGVKAEKTNYESIENLKKVTEESPVRCYDEEAGRKMMDAIDEAKANGDSIGGIVEVIVEGVPVGVGSYVHYDRK
LDSKLAAAVLSINAFKGVEFGIGFEAARKNGSEVHDEIIWDEEKGYTRATNRLGGLEGGMTTGMPIVVRGVMKPIPTLYK
PLKSVDIETKEPFTASIERSDSCAVPAASVVAEAVVAWEIANAIVEQFGVDQMDRIAENVEKMRKLAREF
>Mature_390_residues
MRYLTAGESHGPQLTTIIEGVPAGLYITKEDIDYELQRRQKGHGRGRRMQIEKDQARITSGVRHGRSLGSPIALVVENND
WKHWTKIMGSEPITAEEESEMKRQISRPRPGHADLNGAIKYGHRDMRNVLERSSARETTVRVAAGAVAKKILSQLGIQVA
GHVLEIGGVKAEKTNYESIENLKKVTEESPVRCYDEEAGRKMMDAIDEAKANGDSIGGIVEVIVEGVPVGVGSYVHYDRK
LDSKLAAAVLSINAFKGVEFGIGFEAARKNGSEVHDEIIWDEEKGYTRATNRLGGLEGGMTTGMPIVVRGVMKPIPTLYK
PLKSVDIETKEPFTASIERSDSCAVPAASVVAEAVVAWEIANAIVEQFGVDQMDRIAENVEKMRKLAREF

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; seventh step. [C]

COG id: COG0082

COG function: function code E; Chorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788669, Length=372, Percent_Identity=36.2903225806452, Blast_Score=181, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6321290, Length=376, Percent_Identity=33.5106382978723, Blast_Score=191, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000453
- InterPro:   IPR020541 [H]

Pfam domain/function: PF01264 Chorismate_synt [H]

EC number: =4.2.3.5 [H]

Molecular weight: Translated: 42835; Mature: 42835

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00787 CHORISMATE_SYNTHASE_1 ; PS00788 CHORISMATE_SYNTHASE_2 ; PS00789 CHORISMATE_SYNTHASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYLTAGESHGPQLTTIIEGVPAGLYITKEDIDYELQRRQKGHGRGRRMQIEKDQARITS
CCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEHHHHHHHHH
GVRHGRSLGSPIALVVENNDWKHWTKIMGSEPITAEEESEMKRQISRPRPGHADLNGAIK
HHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHH
YGHRDMRNVLERSSARETTVRVAAGAVAKKILSQLGIQVAGHVLEIGGVKAEKTNYESIE
HCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
NLKKVTEESPVRCYDEEAGRKMMDAIDEAKANGDSIGGIVEVIVEGVPVGVGSYVHYDRK
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
LDSKLAAAVLSINAFKGVEFGIGFEAARKNGSEVHDEIIWDEEKGYTRATNRLGGLEGGM
HHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCC
TTGMPIVVRGVMKPIPTLYKPLKSVDIETKEPFTASIERSDSCAVPAASVVAEAVVAWEI
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHH
ANAIVEQFGVDQMDRIAENVEKMRKLAREF
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRYLTAGESHGPQLTTIIEGVPAGLYITKEDIDYELQRRQKGHGRGRRMQIEKDQARITS
CCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEHHHHHHHHH
GVRHGRSLGSPIALVVENNDWKHWTKIMGSEPITAEEESEMKRQISRPRPGHADLNGAIK
HHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHH
YGHRDMRNVLERSSARETTVRVAAGAVAKKILSQLGIQVAGHVLEIGGVKAEKTNYESIE
HCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
NLKKVTEESPVRCYDEEAGRKMMDAIDEAKANGDSIGGIVEVIVEGVPVGVGSYVHYDRK
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
LDSKLAAAVLSINAFKGVEFGIGFEAARKNGSEVHDEIIWDEEKGYTRATNRLGGLEGGM
HHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCC
TTGMPIVVRGVMKPIPTLYKPLKSVDIETKEPFTASIERSDSCAVPAASVVAEAVVAWEI
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHH
ANAIVEQFGVDQMDRIAENVEKMRKLAREF
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA