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The map label for this gene is nth [H]

Identifier: 52786113

GI number: 52786113

Start: 2315345

End: 2316007

Strand: Reverse

Name: nth [H]

Synonym: BLi02369

Alternate gene names: 52786113

Gene position: 2316007-2315345 (Counterclockwise)

Preceding gene: 52786114

Following gene: 52786112

Centisome position: 54.85

GC content: 46.46

Gene sequence:

>663_bases
ATGCTAACAAAAAAACAGATCGAGTTTTGTTTAGACACAATCGGTGAAATGTTTCCGGATGCAGAGTGCGAATTAGTCCA
TGACAATCCGTTTGAACTTGTCATCGCTGTCGCTTTATCGGCCCAGTGCACAGACGCGCTTGTCAATAAAGTGACGAAAA
CGCTTTTCCAAAAATACAAAAAACCTGAAGATTATCTTGCAGTTCCGCTTGAAGAGCTGCAGCAGGACATCAAATCGATC
GGCTTATACCGCAACAAAGCTAAAAATATTCAAAAGCTGTGCAAAATGCTGCTGGAAGACTACGGAGGTGAAGTGCCGAG
AGACCGCGATGAGCTGGTGAAACTTCCGGGTGTCGGAAGAAAGACCGCTAATGTCGTCGTTTCCGTGGCATTCGGCGTTC
CTGCCATTGCCGTCGACACCCATGTGGAAAGAGTTTCAAAAAGGCTCGGCATTTGTAGATGGAAGGATTCGGTCATGGAA
GTTGAAAAGACGCTGATGAAAAAAGTGCCTGAATCAGAGTGGTCCGTCACTCATCACCGCTTGATTTTTTTCGGCCGCTA
TCACTGTAAGGCGCAAAGACCGAAATGCGAAGAATGCCCGTTGTTTTCTCTGTGCAGGGAAGGGCAAAAACGATATAAAA
AAGGGCTGGTGAAGCTGCTGTGA

Upstream 100 bases:

>100_bases
TTCAGGCGGAAGACTGCTCCGCGGCAAAATGAGCAAAAGGAGTATACAAGACAGGTTCCTTTTTACAATTGGCTTGAACA
ATAAAGTGAAAGGTGAAGAC

Downstream 100 bases:

>100_bases
CAAGCGTTTCACTGTTGGACGAATACGAAAAAACGCTGCGGAAGAAAAGGCCGGAATCCGGCGAAAGGCTTGCGGTACTT
TCTGAAAATCCGCTCTGCTA

Product: hypothetical protein

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MLTKKQIEFCLDTIGEMFPDAECELVHDNPFELVIAVALSAQCTDALVNKVTKTLFQKYKKPEDYLAVPLEELQQDIKSI
GLYRNKAKNIQKLCKMLLEDYGGEVPRDRDELVKLPGVGRKTANVVVSVAFGVPAIAVDTHVERVSKRLGICRWKDSVME
VEKTLMKKVPESEWSVTHHRLIFFGRYHCKAQRPKCEECPLFSLCREGQKRYKKGLVKLL

Sequences:

>Translated_220_residues
MLTKKQIEFCLDTIGEMFPDAECELVHDNPFELVIAVALSAQCTDALVNKVTKTLFQKYKKPEDYLAVPLEELQQDIKSI
GLYRNKAKNIQKLCKMLLEDYGGEVPRDRDELVKLPGVGRKTANVVVSVAFGVPAIAVDTHVERVSKRLGICRWKDSVME
VEKTLMKKVPESEWSVTHHRLIFFGRYHCKAQRPKCEECPLFSLCREGQKRYKKGLVKLL
>Mature_220_residues
MLTKKQIEFCLDTIGEMFPDAECELVHDNPFELVIAVALSAQCTDALVNKVTKTLFQKYKKPEDYLAVPLEELQQDIKSI
GLYRNKAKNIQKLCKMLLEDYGGEVPRDRDELVKLPGVGRKTANVVVSVAFGVPAIAVDTHVERVSKRLGICRWKDSVME
VEKTLMKKVPESEWSVTHHRLIFFGRYHCKAQRPKCEECPLFSLCREGQKRYKKGLVKLL

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=184, Percent_Identity=32.0652173913043, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI115298648, Length=193, Percent_Identity=28.4974093264249, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI190358497, Length=193, Percent_Identity=28.4974093264249, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI6912520, Length=193, Percent_Identity=28.4974093264249, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI115298654, Length=193, Percent_Identity=28.4974093264249, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI115298652, Length=193, Percent_Identity=28.4974093264249, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI115298650, Length=193, Percent_Identity=28.4974093264249, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1787920, Length=211, Percent_Identity=47.8672985781991, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1789331, Length=185, Percent_Identity=25.9459459459459, Blast_Score=68, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17554540, Length=179, Percent_Identity=38.5474860335196, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319304, Length=182, Percent_Identity=27.4725274725275, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324530, Length=191, Percent_Identity=26.1780104712042, Blast_Score=66, Evalue=5e-12,
Organism=Drosophila melanogaster, GI45550361, Length=188, Percent_Identity=32.4468085106383, Blast_Score=100, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 25184; Mature: 25184

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKKQIEFCLDTIGEMFPDAECELVHDNPFELVIAVALSAQCTDALVNKVTKTLFQKYK
CCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KPEDYLAVPLEELQQDIKSIGLYRNKAKNIQKLCKMLLEDYGGEVPRDRDELVKLPGVGR
CCCHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCC
KTANVVVSVAFGVPAIAVDTHVERVSKRLGICRWKDSVMEVEKTLMKKVPESEWSVTHHR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LIFFGRYHCKAQRPKCEECPLFSLCREGQKRYKKGLVKLL
HHEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTKKQIEFCLDTIGEMFPDAECELVHDNPFELVIAVALSAQCTDALVNKVTKTLFQKYK
CCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KPEDYLAVPLEELQQDIKSIGLYRNKAKNIQKLCKMLLEDYGGEVPRDRDELVKLPGVGR
CCCHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCC
KTANVVVSVAFGVPAIAVDTHVERVSKRLGICRWKDSVMEVEKTLMKKVPESEWSVTHHR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LIFFGRYHCKAQRPKCEECPLFSLCREGQKRYKKGLVKLL
HHEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]