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The map label for this gene is yojI

Identifier: 52786009

GI number: 52786009

Start: 2226615

End: 2227973

Strand: Direct

Name: yojI

Synonym: BLi02265

Alternate gene names: 52786009

Gene position: 2226615-2227973 (Clockwise)

Preceding gene: 52786002

Following gene: 52786014

Centisome position: 52.73

GC content: 48.05

Gene sequence:

>1359_bases
ATGAGGGAAACAAAGGATTTGCGGGAGAAAGCAAGACAGTTTTTAACGATTTTAATTCCGATTTTGATTACGCAAGCGGG
ACTGTCGCTCATTACGGTTCTCGACACGGTGATGTCAGGCAAAGTCAGCGCCGCCGACCTCGCCGGCGTCGCAATCGGCT
CGAGTTTATGGACCCCCGTTTATACTGGGCTTGCCGGCATTTTGACGGCTGTAACACCTATGGTCGCCCAGCTGATGGGT
GCCAAGCGGCAAAAGGATGTCCCTTATACTGTTATTCAATCGATCTATGTGGCCGGAATCATCAGCCTTGCCGTCATCGT
TTCCGGATATTTTCTGATTGATCCGGTGCTGGAGAATCTCGATCTTGAACGGAAGGTAGCTGTCATCGCAAAGCAATATT
TAATCTGCATCGGGCTAGGCATATTGCCGCTGTTTGTTTACAATGTGATGCGCTGCTTTATTGATTCACTCGGGAAAACG
CGGGTGACGATGCTGATTACGCTTTGTTCTTTGCCCATTAATTTTGTCTTGAACTATTTGTTTATTTTCGGGAACTTCGG
GTTTCCAAAGCTTGGAGGAGCAGGAGCCGGTTTGGCTTCCGCTATCACATATTGGTGCATTTGTCTGATCTCTTTATACA
TCGTTCATAAAAAGACGCCGTTTCACCAGTTTAGGATTTTTGGAACATTCTATTCATTTTCTTTTGCTGAAAGTATGAAA
CTTCTGAAGATCGGCATCCCGATCGGTTTCGCCATCTTCTTTGAGACGAGTATATTCGCAGCTGTCACCTTGCTGATGAG
CCACTTTGACACGGTCACGATCGCTTCCCATCAGGCGGCAATGAATTTTGCTTCGCTTCTTTACATGCTTCCATTGAGCG
TATCGATGACGCTGACGATTGTCGTCGGCTTTGAAGCCGGGGCCAAACGGTTTAAAGACTCGAGGGCCTACAGCTATCTC
GGCATTTCAATAGCGGTCGGTTTTTCATTATTCACCGCACTCATCATACTACTGTTCAGAGAGCAGATCGCCGGTTTGTA
CGCGGCTGACCGGGATGTCCTGCTTCTGACCAAGGACTTCTTGCTGTATGCGTTGTTTTTTCAGCTGTCAGATGCCATTG
CCGCGCCGATTCAAGGAGCGCTCAGAGGATATAAAGATGTCAACTACACGCTTGTCACAGCTCTCGTCTCATATTGGATT
ATCGGGCTTCCGGTCGGTTTTGTAATCGGAACGTATACTTCATTCGGAGCTTTCGGATACTGGATCGGGCTGATCACGGG
GCTAGCGGCCGGTGCAGTCGGGCTGTTCTTCAGGCTCAAACGCATTCAAAACCGCTATATTCACACTCAAAGCATGTAA

Upstream 100 bases:

>100_bases
AAAACAAAACAGACGAAAAAAGATTGAACAGACATTTATTGCATTTTATACATAACGTGTTATAATTTCTCTCGTTATCC
AAAAGAAACGGGGAACTGAT

Downstream 100 bases:

>100_bases
AAAAGGTGCCGGGCTCAAATGCCCGACACCTTTTTTGATCATGTTAATCATTCCATTGATAGGTCCAAAATGCTTCTTTT
TCAAACCATGTCTCAACAAC

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MRETKDLREKARQFLTILIPILITQAGLSLITVLDTVMSGKVSAADLAGVAIGSSLWTPVYTGLAGILTAVTPMVAQLMG
AKRQKDVPYTVIQSIYVAGIISLAVIVSGYFLIDPVLENLDLERKVAVIAKQYLICIGLGILPLFVYNVMRCFIDSLGKT
RVTMLITLCSLPINFVLNYLFIFGNFGFPKLGGAGAGLASAITYWCICLISLYIVHKKTPFHQFRIFGTFYSFSFAESMK
LLKIGIPIGFAIFFETSIFAAVTLLMSHFDTVTIASHQAAMNFASLLYMLPLSVSMTLTIVVGFEAGAKRFKDSRAYSYL
GISIAVGFSLFTALIILLFREQIAGLYAADRDVLLLTKDFLLYALFFQLSDAIAAPIQGALRGYKDVNYTLVTALVSYWI
IGLPVGFVIGTYTSFGAFGYWIGLITGLAAGAVGLFFRLKRIQNRYIHTQSM

Sequences:

>Translated_452_residues
MRETKDLREKARQFLTILIPILITQAGLSLITVLDTVMSGKVSAADLAGVAIGSSLWTPVYTGLAGILTAVTPMVAQLMG
AKRQKDVPYTVIQSIYVAGIISLAVIVSGYFLIDPVLENLDLERKVAVIAKQYLICIGLGILPLFVYNVMRCFIDSLGKT
RVTMLITLCSLPINFVLNYLFIFGNFGFPKLGGAGAGLASAITYWCICLISLYIVHKKTPFHQFRIFGTFYSFSFAESMK
LLKIGIPIGFAIFFETSIFAAVTLLMSHFDTVTIASHQAAMNFASLLYMLPLSVSMTLTIVVGFEAGAKRFKDSRAYSYL
GISIAVGFSLFTALIILLFREQIAGLYAADRDVLLLTKDFLLYALFFQLSDAIAAPIQGALRGYKDVNYTLVTALVSYWI
IGLPVGFVIGTYTSFGAFGYWIGLITGLAAGAVGLFFRLKRIQNRYIHTQSM
>Mature_452_residues
MRETKDLREKARQFLTILIPILITQAGLSLITVLDTVMSGKVSAADLAGVAIGSSLWTPVYTGLAGILTAVTPMVAQLMG
AKRQKDVPYTVIQSIYVAGIISLAVIVSGYFLIDPVLENLDLERKVAVIAKQYLICIGLGILPLFVYNVMRCFIDSLGKT
RVTMLITLCSLPINFVLNYLFIFGNFGFPKLGGAGAGLASAITYWCICLISLYIVHKKTPFHQFRIFGTFYSFSFAESMK
LLKIGIPIGFAIFFETSIFAAVTLLMSHFDTVTIASHQAAMNFASLLYMLPLSVSMTLTIVVGFEAGAKRFKDSRAYSYL
GISIAVGFSLFTALIILLFREQIAGLYAADRDVLLLTKDFLLYALFFQLSDAIAAPIQGALRGYKDVNYTLVTALVSYWI
IGLPVGFVIGTYTSFGAFGYWIGLITGLAAGAVGLFFRLKRIQNRYIHTQSM

Specific function: Multidrug efflux pump

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Homologues:

Organism=Homo sapiens, GI153792564, Length=408, Percent_Identity=21.078431372549, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI22907060, Length=425, Percent_Identity=21.4117647058824, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI38261960, Length=254, Percent_Identity=24.0157480314961, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI48994890, Length=444, Percent_Identity=33.7837837837838, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI226510954, Length=452, Percent_Identity=23.0088495575221, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321821, Length=374, Percent_Identity=25.1336898395722, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320545, Length=419, Percent_Identity=19.5704057279236, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NORM_BACLD (Q65IG9)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_079426.1
- RefSeq:   YP_091838.1
- STRING:   Q65IG9
- EnsemblBacteria:   EBBACT00000057331
- EnsemblBacteria:   EBBACT00000060662
- GeneID:   3027280
- GeneID:   3100819
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02265
- KEGG:   bli:BL01465
- NMPDR:   fig|279010.5.peg.35
- eggNOG:   COG0534
- GeneTree:   EBGT00050000000379
- HOGENOM:   HBG479615
- OMA:   PINFVLN
- ProtClustDB:   PRK01766
- BioCyc:   BLIC279010-1:BLI02265-MONOMER
- BioCyc:   BLIC279010:BL01465-MONOMER
- InterPro:   IPR002528
- PANTHER:   PTHR11206
- TIGRFAMs:   TIGR00797

Pfam domain/function: PF01554 MatE

EC number: NA

Molecular weight: Translated: 49551; Mature: 49551

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1036743c)-; HASH(0x10000008)-; HASH(0xf145f24)-; HASH(0xea4b918)-; HASH(0xfd37b2c)-; HASH(0x1033dad4)-; HASH(0x101504d8)-; HASH(0x105c3b74)-; HASH(0xfff373c)-; HASH(0xf939258)-; HASH(0x10757114)-; HASH(0xfb2b55c)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA