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The map label for this gene is gltD [H]

Identifier: 52785911

GI number: 52785911

Start: 2111019

End: 2112500

Strand: Reverse

Name: gltD [H]

Synonym: BLi02161

Alternate gene names: 52785911

Gene position: 2112500-2111019 (Counterclockwise)

Preceding gene: 52785912

Following gene: 52785910

Centisome position: 50.03

GC content: 49.46

Gene sequence:

>1482_bases
ATGGGCAAAGCAACAGGATTTATGGAATTCAAGCGGGAAAAGCCTGCTGAACGCGATCCTCTCACACGGATAAACGACTG
GAACGAATATTCGTCTCCATTTTCTGAGGAAGCATCAAAAAGACAGGGAGCGCGCTGCATGGACTGCGGCACCCCCTTTT
GCCAAATCGGCATGGATATAGGCGGTTATACGTCAGGATGTCCGATTCACAATTTAATCCCTGAATGGAACGACCTCGTT
TACAAGGGAAGGTGGAAAGAAGCGCTTGACCGCCTTCTAAAGACAAACAATTTCCCTGAATTCACTGGGCGGGTCTGTCC
GGCGCCTTGTGAGGGCTCTTGTACGGTGGCGATTTCCGATCCGGCCGTATCCATCAAAGGGATCGAACGGACGATTATTG
ACAAAGGATTTGAAAACGGATGGATTCAGCCGCGAATTCCGAAAAAACGGAGCGGCAAGAAAATCGCGATAGTCGGCTCA
GGGCCTGCCGGTCTTGCAAGCGCCGATCAATTGAACCAGGCGGGGCATTCGGTCACTGTTTATGAGCGCAGCGACCGCCC
GGGAGGCCTGTTAATGTACGGCATCCCGAGCATGAAGCTCGAAAAAAGCGTTGTCGAACGCCGCATCAAACTGTTGACAC
AGGAAGGCGTCGACTTTGTCACCAATACGGAAATCGGCAAAGATATTACAGCCGACGAACTAAAAGAACAGTACGATGCG
ATTATCCTTTGCACAGGGGCCCAAAAACAGCGCGATCTCTTGATTGAAGGCCGTGAAGCAAAAGGTGTCCACTTTGCCAT
GGACTATTTAACAGGCACCATTAAAAGCATGCTCGATTCGAATTTTAAAGATAAGCAGTTTATCAATACGAAAAATAAAG
ACGTCATCGTCATCGGCGGCGGTGACACGGGGGCAGACTGCGTTGCAACGGCCCTGCGCCAAAAAGCGCGAAGCGTCGTC
CAATTCGGCAAGCATCCGAAGCTGCCCGATACAAGGACCGCCGAAAATATGTGGCCGGAACAGCCGCATGTCCATACGCT
GGACTATGCCTATGAAGAGGCGCAAGCAGTATTCGGAAACGATCCGAGACAATATTCGATCCAAACAAAAAAGCTTGTCG
CAGATAAAAACGGCCATTTGAAAGAACTGCATACGATTCAAATGAAAAAAGTAAAGAATGATCAAGGGAAATTTGAGTTC
CACGAAATTCCGGGAACGGAAAAAGTATGGCCTGCTCAATACGTCTTTATCGCCATCGGCTTTGAAGGAACGGAACAGCC
GCTGCTTGAGCAGTTCGGGGTAAATACGGTGAGAAATAAAGTTGACGCAGCATACGGGGAATTTAAAACAAATGTCGATG
GAGTCTTCGCAGCAGGGGATGCGAGAAGAGGACAAAGCCTGATCGTTTGGGCGATTCACGAGGGACGCGAAGTCGCCCGT
GAAGTTGACAAATATTTAATGGGGAGCTCTGTACTGCCTTAA

Upstream 100 bases:

>100_bases
TTGATGTACGCGTTTGAAGCCGGTACGAAACAAAAGATTGAACCATCTGCGGCTGCTTCATCAAAAGAAGCGGTGGCACA
GTAAGAAAGGGGAGGGGAAA

Downstream 100 bases:

>100_bases
AAACAAAGGGGTATTCACATCATATAAATGAAAGCCTGTCCCGCGACCTTATTTTTAGGTTGACGGGGCAGGCTGTTTTG
TTTTTTTGATTCAGTGCTTT

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MGKATGFMEFKREKPAERDPLTRINDWNEYSSPFSEEASKRQGARCMDCGTPFCQIGMDIGGYTSGCPIHNLIPEWNDLV
YKGRWKEALDRLLKTNNFPEFTGRVCPAPCEGSCTVAISDPAVSIKGIERTIIDKGFENGWIQPRIPKKRSGKKIAIVGS
GPAGLASADQLNQAGHSVTVYERSDRPGGLLMYGIPSMKLEKSVVERRIKLLTQEGVDFVTNTEIGKDITADELKEQYDA
IILCTGAQKQRDLLIEGREAKGVHFAMDYLTGTIKSMLDSNFKDKQFINTKNKDVIVIGGGDTGADCVATALRQKARSVV
QFGKHPKLPDTRTAENMWPEQPHVHTLDYAYEEAQAVFGNDPRQYSIQTKKLVADKNGHLKELHTIQMKKVKNDQGKFEF
HEIPGTEKVWPAQYVFIAIGFEGTEQPLLEQFGVNTVRNKVDAAYGEFKTNVDGVFAAGDARRGQSLIVWAIHEGREVAR
EVDKYLMGSSVLP

Sequences:

>Translated_493_residues
MGKATGFMEFKREKPAERDPLTRINDWNEYSSPFSEEASKRQGARCMDCGTPFCQIGMDIGGYTSGCPIHNLIPEWNDLV
YKGRWKEALDRLLKTNNFPEFTGRVCPAPCEGSCTVAISDPAVSIKGIERTIIDKGFENGWIQPRIPKKRSGKKIAIVGS
GPAGLASADQLNQAGHSVTVYERSDRPGGLLMYGIPSMKLEKSVVERRIKLLTQEGVDFVTNTEIGKDITADELKEQYDA
IILCTGAQKQRDLLIEGREAKGVHFAMDYLTGTIKSMLDSNFKDKQFINTKNKDVIVIGGGDTGADCVATALRQKARSVV
QFGKHPKLPDTRTAENMWPEQPHVHTLDYAYEEAQAVFGNDPRQYSIQTKKLVADKNGHLKELHTIQMKKVKNDQGKFEF
HEIPGTEKVWPAQYVFIAIGFEGTEQPLLEQFGVNTVRNKVDAAYGEFKTNVDGVFAAGDARRGQSLIVWAIHEGREVAR
EVDKYLMGSSVLP
>Mature_492_residues
GKATGFMEFKREKPAERDPLTRINDWNEYSSPFSEEASKRQGARCMDCGTPFCQIGMDIGGYTSGCPIHNLIPEWNDLVY
KGRWKEALDRLLKTNNFPEFTGRVCPAPCEGSCTVAISDPAVSIKGIERTIIDKGFENGWIQPRIPKKRSGKKIAIVGSG
PAGLASADQLNQAGHSVTVYERSDRPGGLLMYGIPSMKLEKSVVERRIKLLTQEGVDFVTNTEIGKDITADELKEQYDAI
ILCTGAQKQRDLLIEGREAKGVHFAMDYLTGTIKSMLDSNFKDKQFINTKNKDVIVIGGGDTGADCVATALRQKARSVVQ
FGKHPKLPDTRTAENMWPEQPHVHTLDYAYEEAQAVFGNDPRQYSIQTKKLVADKNGHLKELHTIQMKKVKNDQGKFEFH
EIPGTEKVWPAQYVFIAIGFEGTEQPLLEQFGVNTVRNKVDAAYGEFKTNVDGVFAAGDARRGQSLIVWAIHEGREVARE
VDKYLMGSSVLP

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=302, Percent_Identity=26.4900662251656, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI111118981, Length=119, Percent_Identity=35.2941176470588, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI111118983, Length=119, Percent_Identity=35.2941176470588, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1789606, Length=488, Percent_Identity=35.4508196721311, Blast_Score=278, Evalue=6e-76,
Organism=Escherichia coli, GI1788811, Length=479, Percent_Identity=35.4906054279749, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI87082180, Length=428, Percent_Identity=32.9439252336449, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1788468, Length=468, Percent_Identity=23.5042735042735, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789243, Length=432, Percent_Identity=26.6203703703704, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17570289, Length=498, Percent_Identity=48.3935742971888, Blast_Score=482, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI71984108, Length=318, Percent_Identity=27.0440251572327, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17543860, Length=184, Percent_Identity=33.1521739130435, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320030, Length=496, Percent_Identity=48.991935483871, Blast_Score=499, Evalue=1e-142,
Organism=Drosophila melanogaster, GI28574881, Length=490, Percent_Identity=50.2040816326531, Blast_Score=518, Evalue=1e-147,
Organism=Drosophila melanogaster, GI24665539, Length=490, Percent_Identity=50.2040816326531, Blast_Score=518, Evalue=1e-147,
Organism=Drosophila melanogaster, GI24665547, Length=490, Percent_Identity=50.2040816326531, Blast_Score=517, Evalue=1e-147,
Organism=Drosophila melanogaster, GI24665543, Length=490, Percent_Identity=50.2040816326531, Blast_Score=517, Evalue=1e-147,
Organism=Drosophila melanogaster, GI24640763, Length=327, Percent_Identity=27.5229357798165, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI18858217, Length=327, Percent_Identity=27.5229357798165, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54854; Mature: 54723

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKATGFMEFKREKPAERDPLTRINDWNEYSSPFSEEASKRQGARCMDCGTPFCQIGMDI
CCCCCCCHHHHHCCCCCCCCCCCCCCCHHHCCCHHHHHHHHCCCEEECCCCCHHHHCCCC
GGYTSGCPIHNLIPEWNDLVYKGRWKEALDRLLKTNNFPEFTGRVCPAPCEGSCTVAISD
CCCCCCCCHHHCCCCHHHHEECCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEEECC
PAVSIKGIERTIIDKGFENGWIQPRIPKKRSGKKIAIVGSGPAGLASADQLNQAGHSVTV
CCEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHCCCEEEE
YERSDRPGGLLMYGIPSMKLEKSVVERRIKLLTQEGVDFVTNTEIGKDITADELKEQYDA
EECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHCCE
IILCTGAQKQRDLLIEGREAKGVHFAMDYLTGTIKSMLDSNFKDKQFINTKNKDVIVIGG
EEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCEEEEEEC
GDTGADCVATALRQKARSVVQFGKHPKLPDTRTAENMWPEQPHVHTLDYAYEEAQAVFGN
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCEEEHHHHHHHHHHHHCC
DPRQYSIQTKKLVADKNGHLKELHTIQMKKVKNDQGKFEFHEIPGTEKVWPAQYVFIAIG
CCCEEEEEHHHHEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEEE
FEGTEQPLLEQFGVNTVRNKVDAAYGEFKTNVDGVFAAGDARRGQSLIVWAIHEGREVAR
CCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCHHHHH
EVDKYLMGSSVLP
HHHHHHHCCCCCC
>Mature Secondary Structure 
GKATGFMEFKREKPAERDPLTRINDWNEYSSPFSEEASKRQGARCMDCGTPFCQIGMDI
CCCCCCHHHHHCCCCCCCCCCCCCCCHHHCCCHHHHHHHHCCCEEECCCCCHHHHCCCC
GGYTSGCPIHNLIPEWNDLVYKGRWKEALDRLLKTNNFPEFTGRVCPAPCEGSCTVAISD
CCCCCCCCHHHCCCCHHHHEECCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEEECC
PAVSIKGIERTIIDKGFENGWIQPRIPKKRSGKKIAIVGSGPAGLASADQLNQAGHSVTV
CCEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHCCCEEEE
YERSDRPGGLLMYGIPSMKLEKSVVERRIKLLTQEGVDFVTNTEIGKDITADELKEQYDA
EECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHCCE
IILCTGAQKQRDLLIEGREAKGVHFAMDYLTGTIKSMLDSNFKDKQFINTKNKDVIVIGG
EEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCEEEEEEC
GDTGADCVATALRQKARSVVQFGKHPKLPDTRTAENMWPEQPHVHTLDYAYEEAQAVFGN
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCEEEHHHHHHHHHHHHCC
DPRQYSIQTKKLVADKNGHLKELHTIQMKKVKNDQGKFEFHEIPGTEKVWPAQYVFIAIG
CCCEEEEEHHHHEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEEE
FEGTEQPLLEQFGVNTVRNKVDAAYGEFKTNVDGVFAAGDARRGQSLIVWAIHEGREVAR
CCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCHHHHH
EVDKYLMGSSVLP
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]