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The map label for this gene is yngF [H]

Identifier: 52785855

GI number: 52785855

Start: 2044066

End: 2045127

Strand: Reverse

Name: yngF [H]

Synonym: BLi02102

Alternate gene names: 52785855

Gene position: 2045127-2044066 (Counterclockwise)

Preceding gene: 52785856

Following gene: 52785854

Centisome position: 48.43

GC content: 50.56

Gene sequence:

>1062_bases
ATGTCCGATGACGTGCTGTTTTCCGTCAATCAAAACGGCGCCGCAGCGATTGTTCTGAATCGCCCGAAAGCGCTCAACTC
ACTCACATACGACATGGTCCGTCTGATTGGTGAAAAGTTAAACGAGTGGGAGACAGATCAAAACGTTTCTATCGTGGTCA
TCAAAGGTGCAGGACCAAAAGGACTATGTGCCGGAGGGGATATTAAGGCACTCTATGAAGCTCGTTCGTCAAAACAGGCC
CTGCAAGATGCCGAGCGCTTTTTTGAAACAGAGTACGAAGTCGATATGGCAGTCCATCGATTTTCGAAACCGATCATCGC
CTGCTTGGACGGGATCGTCATGGGGGGAGGCGTCGGCCTGACGTACGGGGCCAGCCACCGGATCGTCACGGAGAGGACAA
AATGGGCGATGCCCGAAATGAATATCGGCTTCTTTCCGGATGTCGGGGCAGCCTATTTTTTAAACAAAGCCCCGGGCCGC
TTAGGGCGGTATCTTGGATTAACGGCGTCTGTCATCCATGCAGCCGACGTGCTGTATATCAATGGGGCAGACGCCTACAT
GGAGAGCGGCGCTTTAGAACGATTGCTTCAAGCAGTGGAACAAACCGATTGGCGCCTTGCAAGCGTTGAAGAAAAGCTCG
ATCAGCTGATCCGCGAATCGAAAACGGAGCCCTCCCAGGAGAGCACGCTCGCCCGTGATCAGCAAGCGATTGACCGTCAT
TTTAAGTATGATAAGCTGGAAGAGATCCTTCAATCGCTCGAAAGCGAGGGAAGCACCTTTAGCTCGAATGTGAAAAAAAC
AATGCTTTCCAAATCGCCATTTTCATTAAAAATCACATTGAAACAGCTGGCGGACGGACGTCAAAAAACACTGGAAGAAT
GCTTTGCCACGGATCTGGTGCTGGCAAAGAACTTTTTGAAGCACAATGATTTCTTCGAAGGCGTCAGGTCCGTCCTGATC
GACCGTGATCAATCACCGAACTACAAGTACCGGAACGTTTCAGATGTAACCGATGAAGCGGTGGACCGGTTTTTCCAACC
CTCTGAATCTGTCCGGTTTTAA

Upstream 100 bases:

>100_bases
CGCCGAGCTGTTTGTCACTTCAGATGCCAAAGAAGGAATTCACGCATTTGTCGAAAAGCGCAAACCAAACTTTCATCATT
CATAAAAGGAGTGGATCAAG

Downstream 100 bases:

>100_bases
AAGCGGGAGCTGACCATTAGCGTCAGCTCTTTTTTTGTAAGAACCTTATTATAGAGGTATATATTTCGATATGGAGAATA
TGTATACTGACAATATTTGG

Product: 3-hydroxyisobutyryl-CoA hydrolase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MSDDVLFSVNQNGAAAIVLNRPKALNSLTYDMVRLIGEKLNEWETDQNVSIVVIKGAGPKGLCAGGDIKALYEARSSKQA
LQDAERFFETEYEVDMAVHRFSKPIIACLDGIVMGGGVGLTYGASHRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGR
LGRYLGLTASVIHAADVLYINGADAYMESGALERLLQAVEQTDWRLASVEEKLDQLIRESKTEPSQESTLARDQQAIDRH
FKYDKLEEILQSLESEGSTFSSNVKKTMLSKSPFSLKITLKQLADGRQKTLEECFATDLVLAKNFLKHNDFFEGVRSVLI
DRDQSPNYKYRNVSDVTDEAVDRFFQPSESVRF

Sequences:

>Translated_353_residues
MSDDVLFSVNQNGAAAIVLNRPKALNSLTYDMVRLIGEKLNEWETDQNVSIVVIKGAGPKGLCAGGDIKALYEARSSKQA
LQDAERFFETEYEVDMAVHRFSKPIIACLDGIVMGGGVGLTYGASHRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGR
LGRYLGLTASVIHAADVLYINGADAYMESGALERLLQAVEQTDWRLASVEEKLDQLIRESKTEPSQESTLARDQQAIDRH
FKYDKLEEILQSLESEGSTFSSNVKKTMLSKSPFSLKITLKQLADGRQKTLEECFATDLVLAKNFLKHNDFFEGVRSVLI
DRDQSPNYKYRNVSDVTDEAVDRFFQPSESVRF
>Mature_352_residues
SDDVLFSVNQNGAAAIVLNRPKALNSLTYDMVRLIGEKLNEWETDQNVSIVVIKGAGPKGLCAGGDIKALYEARSSKQAL
QDAERFFETEYEVDMAVHRFSKPIIACLDGIVMGGGVGLTYGASHRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGRL
GRYLGLTASVIHAADVLYINGADAYMESGALERLLQAVEQTDWRLASVEEKLDQLIRESKTEPSQESTLARDQQAIDRHF
KYDKLEEILQSLESEGSTFSSNVKKTMLSKSPFSLKITLKQLADGRQKTLEECFATDLVLAKNFLKHNDFFEGVRSVLID
RDQSPNYKYRNVSDVTDEAVDRFFQPSESVRF

Specific function: Catalyzes The Reversible Dehydration Of L-Carnitinyl-CoA To Crotobetainyl-CoA. [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=337, Percent_Identity=40.3560830860534, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI37594469, Length=306, Percent_Identity=38.8888888888889, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI194097323, Length=215, Percent_Identity=29.3023255813954, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI221142681, Length=140, Percent_Identity=30.7142857142857, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=145, Percent_Identity=26.2068965517241, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI25144157, Length=345, Percent_Identity=36.5217391304348, Blast_Score=233, Evalue=8e-62,
Organism=Caenorhabditis elegans, GI25144160, Length=345, Percent_Identity=36.5217391304348, Blast_Score=233, Evalue=9e-62,
Organism=Caenorhabditis elegans, GI17540714, Length=225, Percent_Identity=25.3333333333333, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=146, Percent_Identity=32.1917808219178, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=149, Percent_Identity=30.8724832214765, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=360, Percent_Identity=31.9444444444444, Blast_Score=177, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28571730, Length=345, Percent_Identity=37.6811594202899, Blast_Score=244, Evalue=7e-65,
Organism=Drosophila melanogaster, GI28571729, Length=345, Percent_Identity=37.6811594202899, Blast_Score=244, Evalue=7e-65,
Organism=Drosophila melanogaster, GI20129971, Length=161, Percent_Identity=30.4347826086957, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24653477, Length=161, Percent_Identity=30.4347826086957, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24653139, Length=168, Percent_Identity=27.3809523809524, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 39448; Mature: 39317

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDVLFSVNQNGAAAIVLNRPKALNSLTYDMVRLIGEKLNEWETDQNVSIVVIKGAGPK
CCCCEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
GLCAGGDIKALYEARSSKQALQDAERFFETEYEVDMAVHRFSKPIIACLDGIVMGGGVGL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCE
TYGASHRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGRLGRYLGLTASVIHAADVLYI
EECCCCCEEECHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEE
NGADAYMESGALERLLQAVEQTDWRLASVEEKLDQLIRESKTEPSQESTLARDQQAIDRH
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
FKYDKLEEILQSLESEGSTFSSNVKKTMLSKSPFSLKITLKQLADGRQKTLEECFATDLV
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHH
LAKNFLKHNDFFEGVRSVLIDRDQSPNYKYRNVSDVTDEAVDRFFQPSESVRF
HHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SDDVLFSVNQNGAAAIVLNRPKALNSLTYDMVRLIGEKLNEWETDQNVSIVVIKGAGPK
CCCEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
GLCAGGDIKALYEARSSKQALQDAERFFETEYEVDMAVHRFSKPIIACLDGIVMGGGVGL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCE
TYGASHRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGRLGRYLGLTASVIHAADVLYI
EECCCCCEEECHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEE
NGADAYMESGALERLLQAVEQTDWRLASVEEKLDQLIRESKTEPSQESTLARDQQAIDRH
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
FKYDKLEEILQSLESEGSTFSSNVKKTMLSKSPFSLKITLKQLADGRQKTLEECFATDLV
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHH
LAKNFLKHNDFFEGVRSVLIDRDQSPNYKYRNVSDVTDEAVDRFFQPSESVRF
HHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]