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The map label for this gene is narK [H]

Identifier: 52785835

GI number: 52785835

Start: 2022508

End: 2023692

Strand: Reverse

Name: narK [H]

Synonym: BLi02081

Alternate gene names: 52785835

Gene position: 2023692-2022508 (Counterclockwise)

Preceding gene: 52785839

Following gene: 52785834

Centisome position: 47.92

GC content: 48.69

Gene sequence:

>1185_bases
ATGGCAAATCGTCAAAAAATACAATTACCGCTGCAGTCATTTAGTTTAGTCGCAGGATTTATGGTTTGGGTATTGATTTC
CTCTTTAATGCCCCACATTAAGCAGGACATCGCCCTGACAGACAGCGAGGTCTCCCTTGTTACGGCCATTCCGGTCATAC
TCGGGTCGCTTCTCAGGATTCCGCTGGGCTACTACACGAACAGATTTGGAGCAAGAATTACATTCATGATCAGCTTTTTG
CTGCTTCTGTTCCCGGTTTTTTGGATCAGCACAGCCGATTCGCTGTTCGACTTGATCCTTGGCGGCTTCTTCCTCGGCAT
CGGCGGAGCGGTCTTTTCCATCGGGGTCACATCACTGCCGAAATACTACCCTAAAGAAAAGCACGGGTTTGTCAATGGGA
TCTACGGCGCTGGGAACGTCGGTACAGCGATTACTACGTTTGCCGCTCCTGTTGTCGCAAAAACCGCCGGGTGGGAAAAT
ACGGTGCGGATGTACATCGTGTTAGTCGCCGTATTCGCTTTATTGAACTTTATCCTTGGAGATCGAAAAGAAACAAAAGT
TACGGTATCTGTAACAGAGCAAATGAAGGCAGTCTACCGAAATCAGACATTATGGTTTTTAAGCTTGTTTTACTTCATTA
CATTCGGATCTTTCGTCGCGTTTACGATTTATCTTCCGAACTTTCTTGTTAATCACTTCGGGCTTGATCCCGTCGATTCA
GGGCTGAGAACCGCCGGATTTATCGCTGTCGCCACTTTTCTGAGGCCTGTCGGCGGCTGGCTGGCAGACAAATTCAACCC
GCTGAAGCTGTTGATGTATGTATTTGCCGGCTGCACCTTGTCAGGGATTTTATTGGCCTTTTCGCCGAGCCTCGGACTGT
ACGGGTTTGGGGTATTAACCGTTGCCGTGTGCGCCGGACTCGGAAACGGGATCATCTTTAAGCTCGTGCCGCTTTATTTT
TCAAATCAGGCCGGCATCGTTAATGGGATCGTATCAGCGATGGGCGGCTTGGGAGGATTTTTCCCGCCTCTCATCCTGTC
AAGCGTTTTTCAGGCAACCGGCCATTATGCGATCGGATTCATGGCGCTGTCAGAAGCGGCTCTTGCAAGCTTTGTGATCG
TGGTCTGGATGTATTTTCAGGAAGTGAACAAAACATCGTCCAAAACGAAGGCCAACCATTTATAA

Upstream 100 bases:

>100_bases
GACCGTGTGACGTAATTCACAATGTTTTAAAAAGACTTTTTGTAAGATAAACATGCTGATTGATTTATTCATTTTTCCCT
TTGAAAGGAGCAAAACCGCA

Downstream 100 bases:

>100_bases
GCCCTTCCTGCAAAAAGAGATTGAGCCGATATGCCCAGGCTCTTTTTCGGGACTATATTCATATGTTCACAATATTGTTT
ATATTTAAAAGGTCACTCTG

Product: hypothetical protein

Products: NA

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MANRQKIQLPLQSFSLVAGFMVWVLISSLMPHIKQDIALTDSEVSLVTAIPVILGSLLRIPLGYYTNRFGARITFMISFL
LLLFPVFWISTADSLFDLILGGFFLGIGGAVFSIGVTSLPKYYPKEKHGFVNGIYGAGNVGTAITTFAAPVVAKTAGWEN
TVRMYIVLVAVFALLNFILGDRKETKVTVSVTEQMKAVYRNQTLWFLSLFYFITFGSFVAFTIYLPNFLVNHFGLDPVDS
GLRTAGFIAVATFLRPVGGWLADKFNPLKLLMYVFAGCTLSGILLAFSPSLGLYGFGVLTVAVCAGLGNGIIFKLVPLYF
SNQAGIVNGIVSAMGGLGGFFPPLILSSVFQATGHYAIGFMALSEAALASFVIVVWMYFQEVNKTSSKTKANHL

Sequences:

>Translated_394_residues
MANRQKIQLPLQSFSLVAGFMVWVLISSLMPHIKQDIALTDSEVSLVTAIPVILGSLLRIPLGYYTNRFGARITFMISFL
LLLFPVFWISTADSLFDLILGGFFLGIGGAVFSIGVTSLPKYYPKEKHGFVNGIYGAGNVGTAITTFAAPVVAKTAGWEN
TVRMYIVLVAVFALLNFILGDRKETKVTVSVTEQMKAVYRNQTLWFLSLFYFITFGSFVAFTIYLPNFLVNHFGLDPVDS
GLRTAGFIAVATFLRPVGGWLADKFNPLKLLMYVFAGCTLSGILLAFSPSLGLYGFGVLTVAVCAGLGNGIIFKLVPLYF
SNQAGIVNGIVSAMGGLGGFFPPLILSSVFQATGHYAIGFMALSEAALASFVIVVWMYFQEVNKTSSKTKANHL
>Mature_393_residues
ANRQKIQLPLQSFSLVAGFMVWVLISSLMPHIKQDIALTDSEVSLVTAIPVILGSLLRIPLGYYTNRFGARITFMISFLL
LLFPVFWISTADSLFDLILGGFFLGIGGAVFSIGVTSLPKYYPKEKHGFVNGIYGAGNVGTAITTFAAPVVAKTAGWENT
VRMYIVLVAVFALLNFILGDRKETKVTVSVTEQMKAVYRNQTLWFLSLFYFITFGSFVAFTIYLPNFLVNHFGLDPVDSG
LRTAGFIAVATFLRPVGGWLADKFNPLKLLMYVFAGCTLSGILLAFSPSLGLYGFGVLTVAVCAGLGNGIIFKLVPLYFS
NQAGIVNGIVSAMGGLGGFFPPLILSSVFQATGHYAIGFMALSEAALASFVIVVWMYFQEVNKTSSKTKANHL

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=432, Percent_Identity=26.1574074074074, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42767; Mature: 42636

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure 
ANRQKIQLPLQSFSLVAGFMVWVLISSLMPHIKQDIALTDSEVSLVTAIPVILGSLLRI
CCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]