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The map label for this gene is alsT [H]

Identifier: 52785823

GI number: 52785823

Start: 2008999

End: 2010411

Strand: Direct

Name: alsT [H]

Synonym: BLi02069

Alternate gene names: 52785823

Gene position: 2008999-2010411 (Clockwise)

Preceding gene: 52785819

Following gene: 52785833

Centisome position: 47.58

GC content: 46.92

Gene sequence:

>1413_bases
ATGGAAGAAATTTTAAGCGAAATCATCAATGTCCCGAGCGGTTTTATTTGGACTTATTTAGTCTATATATTAATAGGAAT
AGGCCTGTTCTTTACGTTTAAATTGAAATTCGTTCAATTCCGCTATTTCTTCGAGATGTTTCGTATCGTCGGACAAAAAG
AGGAGAAAGGAAAAGGCGTCTCGTCTTTGCAGGCATTCTTTATTTCCGCCGCTTCCCGTGTCGGAACGGGTAACCTGACC
GGTGTGGCGCTTGCTGTTGCAACAGGCGGACCGGGAGCGGTCTTTTGGATGTGGGTCGTCGCGCTTGTCGGAATGGCTTC
AAGCTTTGTGGAAAGTACGCTGGCTCAAGTCTACAAGGTCAAAGACGGCGATCATTTCAGAGGAGGCCCTGCTTATTACA
TGGAAAAAGCGCTGGGCGCCAGATGGATGGGGATTATCTTTGCCGTTTTAATTACAATTACATTCGGCTTGATTTTTAAT
GCGGTTCAAGCCAACACGATCGTCGGTTCGATGCAAGGGGCTTTCAACCTGGATAAAACAGTGGTAGGCATCATCCTTGC
TGCAATCACCGGATTTATCATTTTCGGCGGATTGAAACGCGTTGTGACAGTTTCGCAGTTGATCGTTCCGGTCATGGCGA
TCATTTATCTTGGACTTGCTTTATTTGTCGTGCTGACGAATATTTCTCAAATACCGGCCGTTTTCGCGATGATTGTGAAG
GATGCTTTCGGGATCGAGCAGATTGCAGGGGGTACGCTCGGCAGCATCATTATTATCGGTGCGAAGCGCGGTTTGTTTTC
AAACGAAGCCGGAATGGGAAGCGCGCCGAACGCAGCAGCGACTGCCAATGTCTCTCACCCCGCGAAGCAAGGCTTTATCC
AAACGCTTGGCGTATTCTTTGATACGATGGTCATCTGCAGTGCAACAGCCTTTATGATCCTGCTGTTCAATCTGACGCCT
GGCAAAGAGCTTGACGGCATTCAAATTACACAAGCAGCCATGCAGCATCACATTGGAAGCTGGGCGCCTGCATTTGTCGC
AGTGGCGATTTTTCTGTTCGCTTTCAGTTCAATCGTCGGAAACTACTATTATGGTGAGACGAACATTGAATTTATTAAAA
AGAGCAAGACGGCGCTTGCTGTTTACCGTACAGCCGTTGTAGCAATGGTGATCTTCGGATCTGTATCCGGTTTCCAGCTT
GTGTGGGACATGGCGGATTTATTTATGGGATTAATGGCGATCATTAACCTGATTGCGATTCTGCTTCTTTCCGGAATTGC
TGCGAAAGTGCTGAAAGACTATACTGAACAGCGCAAAGCAGGAAAGAACCCCGTCTTTAAGGCAAGCTCGATTCCAGGTC
TGAAAAATACAGAGGCTTGGGAAGACGAGAAGGAAACATTGCAATCGAAATAA

Upstream 100 bases:

>100_bases
AAATTCTATCTAGTTTTGCTTGCGTCATTATTATGATATAATCCTATCATGTTAGAAAACTTAACATCAAAACAAAATTC
GTTTGTTTCAGGAGGGTGCT

Downstream 100 bases:

>100_bases
ACCATATTAAAAAAGGCGTCCTTGGGGGCGCCTTTTTCTTATTTTTACTGACAGCTGAAAGAAGTGATCTCGTTCAGGTC
AATACCGAAGTAAACCCAGC

Product: AlsT

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MEEILSEIINVPSGFIWTYLVYILIGIGLFFTFKLKFVQFRYFFEMFRIVGQKEEKGKGVSSLQAFFISAASRVGTGNLT
GVALAVATGGPGAVFWMWVVALVGMASSFVESTLAQVYKVKDGDHFRGGPAYYMEKALGARWMGIIFAVLITITFGLIFN
AVQANTIVGSMQGAFNLDKTVVGIILAAITGFIIFGGLKRVVTVSQLIVPVMAIIYLGLALFVVLTNISQIPAVFAMIVK
DAFGIEQIAGGTLGSIIIIGAKRGLFSNEAGMGSAPNAAATANVSHPAKQGFIQTLGVFFDTMVICSATAFMILLFNLTP
GKELDGIQITQAAMQHHIGSWAPAFVAVAIFLFAFSSIVGNYYYGETNIEFIKKSKTALAVYRTAVVAMVIFGSVSGFQL
VWDMADLFMGLMAIINLIAILLLSGIAAKVLKDYTEQRKAGKNPVFKASSIPGLKNTEAWEDEKETLQSK

Sequences:

>Translated_470_residues
MEEILSEIINVPSGFIWTYLVYILIGIGLFFTFKLKFVQFRYFFEMFRIVGQKEEKGKGVSSLQAFFISAASRVGTGNLT
GVALAVATGGPGAVFWMWVVALVGMASSFVESTLAQVYKVKDGDHFRGGPAYYMEKALGARWMGIIFAVLITITFGLIFN
AVQANTIVGSMQGAFNLDKTVVGIILAAITGFIIFGGLKRVVTVSQLIVPVMAIIYLGLALFVVLTNISQIPAVFAMIVK
DAFGIEQIAGGTLGSIIIIGAKRGLFSNEAGMGSAPNAAATANVSHPAKQGFIQTLGVFFDTMVICSATAFMILLFNLTP
GKELDGIQITQAAMQHHIGSWAPAFVAVAIFLFAFSSIVGNYYYGETNIEFIKKSKTALAVYRTAVVAMVIFGSVSGFQL
VWDMADLFMGLMAIINLIAILLLSGIAAKVLKDYTEQRKAGKNPVFKASSIPGLKNTEAWEDEKETLQSK
>Mature_470_residues
MEEILSEIINVPSGFIWTYLVYILIGIGLFFTFKLKFVQFRYFFEMFRIVGQKEEKGKGVSSLQAFFISAASRVGTGNLT
GVALAVATGGPGAVFWMWVVALVGMASSFVESTLAQVYKVKDGDHFRGGPAYYMEKALGARWMGIIFAVLITITFGLIFN
AVQANTIVGSMQGAFNLDKTVVGIILAAITGFIIFGGLKRVVTVSQLIVPVMAIIYLGLALFVVLTNISQIPAVFAMIVK
DAFGIEQIAGGTLGSIIIIGAKRGLFSNEAGMGSAPNAAATANVSHPAKQGFIQTLGVFFDTMVICSATAFMILLFNLTP
GKELDGIQITQAAMQHHIGSWAPAFVAVAIFLFAFSSIVGNYYYGETNIEFIKKSKTALAVYRTAVVAMVIFGSVSGFQL
VWDMADLFMGLMAIINLIAILLLSGIAAKVLKDYTEQRKAGKNPVFKASSIPGLKNTEAWEDEKETLQSK

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=459, Percent_Identity=45.3159041394335, Blast_Score=364, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 50631; Mature: 50631

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00873 NA_ALANINE_SYMP ; PS00287 CYSTATIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969507; 9384377; 7710280 [H]