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The map label for this gene is yndN

Identifier: 52785283

GI number: 52785283

Start: 1492971

End: 1493435

Strand: Reverse

Name: yndN

Synonym: BLi01518

Alternate gene names: 52785283

Gene position: 1493435-1492971 (Counterclockwise)

Preceding gene: 52785284

Following gene: 52785278

Centisome position: 35.37

GC content: 41.51

Gene sequence:

>465_bases
ATGGAAAACAATAAAATCCTCGGCATCAATCATCTCTTGTTTTCGGTCTCTGACCTGTCCGTCTCTATTTCATTCTATGA
AAAGGTGTTTGATGCCAAGTGGCTCGTAAAAGCCGAGAAAACCGCTTATTTTGACCTGAACGGCATCTGGCTCGCATTCA
ACGAAGAGAAAGACATTAAAAGACAGGAAATCCACGACTCTTACACCCATATCGCGTTTTCGATCCAACAGGAAGATTTG
CCGTTCTGGGAAAAGAAATTACACGATTTAGGCGTTAATGTGCTGAAGGGCAGAAAACGGCATGAAGGAGACAGAGATTC
AATTTATTTCTCAGATCCAGACGGCCATAAGTTCGAGCTTCACACAGGCTCTGTATTTGACCGTCTTCAATACTATCAAA
ATGAAAAACCGCATCTTTCATTTCATGAAGGCCACATCAAGGCTCTTTATGACAGAAACAAATAA

Upstream 100 bases:

>100_bases
GGCTGGCTTGATGATGCGGCTGTGCTCGCTTTTCTCTTCAGGCAAATCACCAAAGAGCTGGACGGGTTTGAAACACATCT
AACAGGGAGAGTAAATTACA

Downstream 100 bases:

>100_bases
AAAGCGTCCCCGGGGACGCTTTTTTTATGACTGGTTCACATTCTCTTTTTCATAAATCGGCACCCAGCCCTCTGTCGTCA
CAAAGATTCTGACAGCCACG

Product: fosfomycin resistance protein FosB

Products: NA

Alternate protein names: Fosfomycin resistance protein

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MENNKILGINHLLFSVSDLSVSISFYEKVFDAKWLVKAEKTAYFDLNGIWLAFNEEKDIKRQEIHDSYTHIAFSIQQEDL
PFWEKKLHDLGVNVLKGRKRHEGDRDSIYFSDPDGHKFELHTGSVFDRLQYYQNEKPHLSFHEGHIKALYDRNK

Sequences:

>Translated_154_residues
MENNKILGINHLLFSVSDLSVSISFYEKVFDAKWLVKAEKTAYFDLNGIWLAFNEEKDIKRQEIHDSYTHIAFSIQQEDL
PFWEKKLHDLGVNVLKGRKRHEGDRDSIYFSDPDGHKFELHTGSVFDRLQYYQNEKPHLSFHEGHIKALYDRNK
>Mature_154_residues
MENNKILGINHLLFSVSDLSVSISFYEKVFDAKWLVKAEKTAYFDLNGIWLAFNEEKDIKRQEIHDSYTHIAFSIQQEDL
PFWEKKLHDLGVNVLKGRKRHEGDRDSIYFSDPDGHKFELHTGSVFDRLQYYQNEKPHLSFHEGHIKALYDRNK

Specific function: Metallothiol transferase which confers resistance to fosfomycin by catalyzing the addition of a thiol cofactor to fosfomycin. L-cysteine is probably the physiological thiol donor

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fosfomycin resistance protein family. FosB subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOSB_BACLD (Q65KJ5)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_078703.1
- RefSeq:   YP_091112.1
- ProteinModelPortal:   Q65KJ5
- SMR:   Q65KJ5
- STRING:   Q65KJ5
- EnsemblBacteria:   EBBACT00000055761
- EnsemblBacteria:   EBBACT00000062542
- GeneID:   3030601
- GeneID:   3098064
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01518
- KEGG:   bli:BL03635
- NMPDR:   fig|279010.5.peg.1070
- eggNOG:   COG0346
- GeneTree:   EBGT00050000001020
- HOGENOM:   HBG704761
- OMA:   QDRLNYY
- ProtClustDB:   PRK04101
- BioCyc:   BLIC279010-1:BLI01518-MONOMER
- BioCyc:   BLIC279010:BL03635-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01512
- InterPro:   IPR004360
- InterPro:   IPR022858

Pfam domain/function: PF00903 Glyoxalase

EC number: NA

Molecular weight: Translated: 18258; Mature: 18258

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENNKILGINHLLFSVSDLSVSISFYEKVFDAKWLVKAEKTAYFDLNGIWLAFNEEKDIK
CCCCEEEEHHHHEEEECCCEEEHHHHHHHHCCCEEEEECCEEEEECCEEEEEECCCCCCH
RQEIHDSYTHIAFSIQQEDLPFWEKKLHDLGVNVLKGRKRHEGDRDSIYFSDPDGHKFEL
HHHHHCCCEEEEEEEECCCCCHHHHHHHHCCCHHHCCCCCCCCCCCEEEEECCCCCEEEE
HTGSVFDRLQYYQNEKPHLSFHEGHIKALYDRNK
ECCHHHHHHHHHCCCCCCEEEECCCEEEEEECCC
>Mature Secondary Structure
MENNKILGINHLLFSVSDLSVSISFYEKVFDAKWLVKAEKTAYFDLNGIWLAFNEEKDIK
CCCCEEEEHHHHEEEECCCEEEHHHHHHHHCCCEEEEECCEEEEECCEEEEEECCCCCCH
RQEIHDSYTHIAFSIQQEDLPFWEKKLHDLGVNVLKGRKRHEGDRDSIYFSDPDGHKFEL
HHHHHCCCEEEEEEEECCCCCHHHHHHHHCCCHHHCCCCCCCCCCCEEEEECCCCCEEEE
HTGSVFDRLQYYQNEKPHLSFHEGHIKALYDRNK
ECCHHHHHHHHHCCCCCCEEEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA