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The map label for this gene is yhaR [H]

Identifier: 52784834

GI number: 52784834

Start: 1064652

End: 1065428

Strand: Direct

Name: yhaR [H]

Synonym: BLi01062

Alternate gene names: 52784834

Gene position: 1064652-1065428 (Clockwise)

Preceding gene: 52784833

Following gene: 52784835

Centisome position: 25.21

GC content: 51.22

Gene sequence:

>777_bases
ATGGAATATATCAAATGGAAAGACGAAGACGGGATTTTTGAAATTGTACTGAATCGCTCTGAGGCGTACAATGCCTTGAA
TGAAAAAATGCTTGAAGAACTGAATGAAGCTTTGCGTGTTGCAGAGGAAAGCGAATCGCTGCTTCTCCTTGTGAGAGGCA
GCGGCAAAGGATTTTCGGCAGGCGGAGACATTAAGATGATGCTGTCTTCCGGAGATCAAGACAGCTCCGCCCGCGTCATT
GACACAATTTCTGAAATTGCGGTGAAGCTTTACAGCATGCCGAAGATGACGACAGCGGCTGTCCACGGTGCGGCTGCGGG
TCTGGGATTGAGCCTCGCGCTCAGCTGCGACCATGTACTGGTCGAAAAAGAGGCGAAGCTGGCGATGAATTTTATCGGCA
TCGGGCTTGTTCCCGACGGAGGCGGACACTTCTTTTTAGAGCGGAGAATCGGTGAAACTGCGGCCAAAGAATTGATTTGG
AGCGGGAAAAAATTGACGGGGGCCGAAGCGCACGAGCTTCGGATCGCAGACGCCGTATTCAGCGGGGACTCCGGCCGTTT
TGCGCGCATCTATCTTGAAAAGCTTCTGCACGCTCCGCTGGCAGCGATGATTGAGACAAAAAAGATCTATCAGGCGTTGA
ATGGAGGCAGGCTGCAGAAAACGCTTGAACTCGAGAAAACGGCCCAGATGAAAATGAGGCTGACAAGCGACCATCAGGAA
GGGATCCGCGCATTTTTAGAAAAGCGCCAGCCGCAATTTAACCGTCAGCAAGTATAA

Upstream 100 bases:

>100_bases
GCGGAATCAGGCCGGAATCGGCAACAAAAAAAGGTTTTGGAGGGCAAAGGTGGAATAATTTAAATAGTATCAATTGTAAA
CGTATTCACGGAGGTGCAAG

Downstream 100 bases:

>100_bases
CAAGAGCGGTCCGGCAGGAAATTGACCTTGGGAAATGATGTGCAGAAACAATTGTAAAAAAATTTAAGTTTCCATAAAAT
GATTCATAAGATACAGATCT

Product: enoyl-CoA hydratase

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MEYIKWKDEDGIFEIVLNRSEAYNALNEKMLEELNEALRVAEESESLLLLVRGSGKGFSAGGDIKMMLSSGDQDSSARVI
DTISEIAVKLYSMPKMTTAAVHGAAAGLGLSLALSCDHVLVEKEAKLAMNFIGIGLVPDGGGHFFLERRIGETAAKELIW
SGKKLTGAEAHELRIADAVFSGDSGRFARIYLEKLLHAPLAAMIETKKIYQALNGGRLQKTLELEKTAQMKMRLTSDHQE
GIRAFLEKRQPQFNRQQV

Sequences:

>Translated_258_residues
MEYIKWKDEDGIFEIVLNRSEAYNALNEKMLEELNEALRVAEESESLLLLVRGSGKGFSAGGDIKMMLSSGDQDSSARVI
DTISEIAVKLYSMPKMTTAAVHGAAAGLGLSLALSCDHVLVEKEAKLAMNFIGIGLVPDGGGHFFLERRIGETAAKELIW
SGKKLTGAEAHELRIADAVFSGDSGRFARIYLEKLLHAPLAAMIETKKIYQALNGGRLQKTLELEKTAQMKMRLTSDHQE
GIRAFLEKRQPQFNRQQV
>Mature_258_residues
MEYIKWKDEDGIFEIVLNRSEAYNALNEKMLEELNEALRVAEESESLLLLVRGSGKGFSAGGDIKMMLSSGDQDSSARVI
DTISEIAVKLYSMPKMTTAAVHGAAAGLGLSLALSCDHVLVEKEAKLAMNFIGIGLVPDGGGHFFLERRIGETAAKELIW
SGKKLTGAEAHELRIADAVFSGDSGRFARIYLEKLLHAPLAAMIETKKIYQALNGGRLQKTLELEKTAQMKMRLTSDHQE
GIRAFLEKRQPQFNRQQV

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=245, Percent_Identity=30.6122448979592, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI4757966, Length=178, Percent_Identity=31.4606741573034, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI51538581, Length=178, Percent_Identity=31.4606741573034, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI25453481, Length=178, Percent_Identity=31.4606741573034, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI51538573, Length=178, Percent_Identity=31.4606741573034, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4757968, Length=178, Percent_Identity=31.4606741573034, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI49355787, Length=178, Percent_Identity=31.4606741573034, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI31542718, Length=255, Percent_Identity=27.4509803921569, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI260274832, Length=179, Percent_Identity=27.3743016759777, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI45643119, Length=179, Percent_Identity=27.3743016759777, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI260275230, Length=179, Percent_Identity=27.3743016759777, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI116235487, Length=178, Percent_Identity=29.2134831460674, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI221136756, Length=178, Percent_Identity=28.6516853932584, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI221136753, Length=178, Percent_Identity=28.6516853932584, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI221307494, Length=178, Percent_Identity=28.6516853932584, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI68989263, Length=197, Percent_Identity=29.4416243654822, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=30.7086614173228, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1787659, Length=245, Percent_Identity=31.8367346938775, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787660, Length=267, Percent_Identity=31.8352059925094, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788597, Length=259, Percent_Identity=26.2548262548263, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI221142681, Length=249, Percent_Identity=28.1124497991968, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1790281, Length=180, Percent_Identity=28.8888888888889, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082183, Length=261, Percent_Identity=26.0536398467433, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788682, Length=177, Percent_Identity=27.683615819209, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=243, Percent_Identity=31.2757201646091, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=33.2, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17540714, Length=186, Percent_Identity=27.9569892473118, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=262, Percent_Identity=24.4274809160305, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25141363, Length=213, Percent_Identity=23.4741784037559, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20129971, Length=262, Percent_Identity=30.1526717557252, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653477, Length=262, Percent_Identity=30.1526717557252, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=27.0916334661355, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28571729, Length=177, Percent_Identity=31.0734463276836, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI28571730, Length=177, Percent_Identity=31.0734463276836, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19920382, Length=196, Percent_Identity=27.0408163265306, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24654903, Length=166, Percent_Identity=23.4939759036145, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI19922422, Length=259, Percent_Identity=26.6409266409266, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 28440; Mature: 28440

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYIKWKDEDGIFEIVLNRSEAYNALNEKMLEELNEALRVAEESESLLLLVRGSGKGFSA
CCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
GGDIKMMLSSGDQDSSARVIDTISEIAVKLYSMPKMTTAAVHGAAAGLGLSLALSCDHVL
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCHHH
VEKEAKLAMNFIGIGLVPDGGGHFFLERRIGETAAKELIWSGKKLTGAEAHELRIADAVF
HHHHHHHHHHHEEEEEEECCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEHHEE
SGDSGRFARIYLEKLLHAPLAAMIETKKIYQALNGGRLQKTLELEKTAQMKMRLTSDHQE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHH
GIRAFLEKRQPQFNRQQV
HHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MEYIKWKDEDGIFEIVLNRSEAYNALNEKMLEELNEALRVAEESESLLLLVRGSGKGFSA
CCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
GGDIKMMLSSGDQDSSARVIDTISEIAVKLYSMPKMTTAAVHGAAAGLGLSLALSCDHVL
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCHHH
VEKEAKLAMNFIGIGLVPDGGGHFFLERRIGETAAKELIWSGKKLTGAEAHELRIADAVF
HHHHHHHHHHHEEEEEEECCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEHHEE
SGDSGRFARIYLEKLLHAPLAAMIETKKIYQALNGGRLQKTLELEKTAQMKMRLTSDHQE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHH
GIRAFLEKRQPQFNRQQV
HHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 9579061 [H]