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The map label for this gene is dat [H]

Identifier: 52784812

GI number: 52784812

Start: 1039241

End: 1040092

Strand: Direct

Name: dat [H]

Synonym: BLi01040

Alternate gene names: 52784812

Gene position: 1039241-1040092 (Clockwise)

Preceding gene: 52784809

Following gene: 52784814

Centisome position: 24.61

GC content: 47.54

Gene sequence:

>852_bases
ATGAAAGTTCTTTTTAACGGCCGGCTGATTGAACGGAGCGAGTGTGCGGTTGATATTGAGGACAGAGGCTACCAATTTGG
AGACGGCGTCTATGAAGTCATCCGCATTTACAATGGAATTTTGTTCACGCTTGATGAACACATTGCCCGCTTATATAAAA
GCGCAGCCGAAATCGGAATCGACCTGTCTTTTTCAGAAGCCGAGCTGAAAAGCCAGCTGAAAGAGCTCGTCGACATCAAT
CAGATGCGGGAAGGCGGGCTGTATCTTCAAGTGACAAGGGGAAAAGCTCCGAGGAAGCATCAATACGGAGCAGGGCTTAC
GCCTCAGGTGACGGCTTATACATTTCCTATTCAGAAGCCTGAAAAAGAACAGCAAAACGGCGTTTCGGCTATTACTGCGG
ACGACATGCGCTGGCTGAGGTGCGACATTAAAAGCTTAAATCTGTTGTACAACGTCATGATCAAGCAAAAAGCGCATGAA
GCTTCTTCATTCGAAGCGATCTTAATTCGCGACGGTTTGGTGACGGAAGGGACGTCTTCAAATGTTTATGTCGTCAAACA
AAACGCTATCTACACCCATCCTGCGACAACGCTCATCTTAAACGGCATCACGCGGATGAAAGTTCTTCAGCTGTGTGAAG
AGAACGGCTTGAATTATGTAGAAAAAGCGGTGACAAAGGATGAATTGCTGAATGCGGATGAAGTATTCATCACGTCAACG
ACAGCCGAAGTCATTCCGGTCACCTCGATTGACGGGCAAACAATCGGCTCCGGAGCGCCCGGACCGCTCACAAAAAATGT
GCAGACCGCTTTGCAAAACAGCATTTTGTCGGAAACAGCCAAAACGGTTTAA

Upstream 100 bases:

>100_bases
AAAAAAGTTTTTGGAAATATTGCACTATTTTGATCGGCAGGGTCGTGTTAGAATGGAATGGAGAAAATTCAGAAGTCAAC
AAAAATAAGGGGATGGAGAA

Downstream 100 bases:

>100_bases
CAAAGAAAAACGCCGTCTCCTGGGGAAACGGCGTTTTTTTATTTGCGGAAACCGAACAAAATCGAAAAGAGCGGCGATAA
ATAAAGGAAAAACACAAACG

Product: Dat

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKVLFNGRLIERSECAVDIEDRGYQFGDGVYEVIRIYNGILFTLDEHIARLYKSAAEIGIDLSFSEAELKSQLKELVDIN
QMREGGLYLQVTRGKAPRKHQYGAGLTPQVTAYTFPIQKPEKEQQNGVSAITADDMRWLRCDIKSLNLLYNVMIKQKAHE
ASSFEAILIRDGLVTEGTSSNVYVVKQNAIYTHPATTLILNGITRMKVLQLCEENGLNYVEKAVTKDELLNADEVFITST
TAEVIPVTSIDGQTIGSGAPGPLTKNVQTALQNSILSETAKTV

Sequences:

>Translated_283_residues
MKVLFNGRLIERSECAVDIEDRGYQFGDGVYEVIRIYNGILFTLDEHIARLYKSAAEIGIDLSFSEAELKSQLKELVDIN
QMREGGLYLQVTRGKAPRKHQYGAGLTPQVTAYTFPIQKPEKEQQNGVSAITADDMRWLRCDIKSLNLLYNVMIKQKAHE
ASSFEAILIRDGLVTEGTSSNVYVVKQNAIYTHPATTLILNGITRMKVLQLCEENGLNYVEKAVTKDELLNADEVFITST
TAEVIPVTSIDGQTIGSGAPGPLTKNVQTALQNSILSETAKTV
>Mature_283_residues
MKVLFNGRLIERSECAVDIEDRGYQFGDGVYEVIRIYNGILFTLDEHIARLYKSAAEIGIDLSFSEAELKSQLKELVDIN
QMREGGLYLQVTRGKAPRKHQYGAGLTPQVTAYTFPIQKPEKEQQNGVSAITADDMRWLRCDIKSLNLLYNVMIKQKAHE
ASSFEAILIRDGLVTEGTSSNVYVVKQNAIYTHPATTLILNGITRMKVLQLCEENGLNYVEKAVTKDELLNADEVFITST
TAEVIPVTSIDGQTIGSGAPGPLTKNVQTALQNSILSETAKTV

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=292, Percent_Identity=27.0547945205479, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787338, Length=261, Percent_Identity=26.4367816091954, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 31334; Mature: 31334

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLFNGRLIERSECAVDIEDRGYQFGDGVYEVIRIYNGILFTLDEHIARLYKSAAEIGI
CEEEECCEEEECCCCEEEECCCCEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
DLSFSEAELKSQLKELVDINQMREGGLYLQVTRGKAPRKHQYGAGLTPQVTAYTFPIQKP
CCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCC
EKEQQNGVSAITADDMRWLRCDIKSLNLLYNVMIKQKAHEASSFEAILIRDGLVTEGTSS
CHHHHCCCEEEECCCHHEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCC
NVYVVKQNAIYTHPATTLILNGITRMKVLQLCEENGLNYVEKAVTKDELLNADEVFITST
CEEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEC
TAEVIPVTSIDGQTIGSGAPGPLTKNVQTALQNSILSETAKTV
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVLFNGRLIERSECAVDIEDRGYQFGDGVYEVIRIYNGILFTLDEHIARLYKSAAEIGI
CEEEECCEEEECCCCEEEECCCCEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
DLSFSEAELKSQLKELVDINQMREGGLYLQVTRGKAPRKHQYGAGLTPQVTAYTFPIQKP
CCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCC
EKEQQNGVSAITADDMRWLRCDIKSLNLLYNVMIKQKAHEASSFEAILIRDGLVTEGTSS
CHHHHCCCEEEECCCHHEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCC
NVYVVKQNAIYTHPATTLILNGITRMKVLQLCEENGLNYVEKAVTKDELLNADEVFITST
CEEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEC
TAEVIPVTSIDGQTIGSGAPGPLTKNVQTALQNSILSETAKTV
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9003455 [H]