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The map label for this gene is yhcA [H]

Identifier: 52784737

GI number: 52784737

Start: 963755

End: 965344

Strand: Direct

Name: yhcA [H]

Synonym: BLi00965

Alternate gene names: 52784737

Gene position: 963755-965344 (Clockwise)

Preceding gene: 52784736

Following gene: 52784738

Centisome position: 22.82

GC content: 48.36

Gene sequence:

>1590_bases
GTGGCTTCTCAGAAAAAAGGAAATATGTCACTGCTTATCATATTAATGGCCGGCTTGTTTCTGGCCATTTTAAACCAAAC
GCTGCTGAATGTGGCGATGCCTCATCTCATGACAGAGTTCAATGTGAGCGCCACAACGATTCAATGGCTGACAACCGGAT
ATATGCTTGTCAACGGCGTTTTGATTCCTTTATCAGCCTTTCTGATTATGCGGTTCGGCGGACGGAGCCTGTTTTTAACG
GCCATGCTGCTGTTTACGGCCGGAACGCTGATATGCGGAATTTCGCCTAATTTCTCGACGATGTTAACCGGACGCCTCAT
TCAGGCAGTCGGCGGAGGCATACTTCAGCCTTTGGTCATGACGACGATTCTCTTTATCTTTCCTCCGGAAAGCCGCGGGA
AAGGAATGGGGATCTTTGGCCTTGCGATGATGTTTGCTCCTGCTGTCGGTCCGACTCTATCCGGCTGGGTCATTGAAAAC
TACAGCTGGCGGATCATGTTTTACAGCCTTGTGCCGGTTGGTGTCATCGTCATCATTTTCGGTTTTTTCCTATTTAAAAA
TATGACGGAACCTAAAAAAATCAAATTGGATACAGCAGGCGCGATTCTTTCAGTCGTCGGATTTGCGGCGTTGCTGTATG
GCGTGAGCGAAGCCGGCTCAGACGGCTGGGATGATCCGATCGTACTTTCTACAGTTGTGATCGGTGTAATCGGCATTGCC
GCTTTTATCATCCAGCAGTTGAAATCGAAAGAACCGATGCTTGACTTCAGAGTGTTCAAATACGATATGTTTTCACTGTC
AAGCGTGATCAACGCCATCATTACGGTTGCTTTGTATGCCGGAATGTTCCTGATTCCGATTTATTTGCAAAACCTGGTCG
GTTTTACAGCGCTTCAGTCGGGGCTTCTTATGCTGCCGGGCGCACTCGTCATGCTTGTGATGTCGCCGATCTCAGGAATT
TTATTTGATAAAGTCGGCCCAAGGCCGCTGGCGATTGTCGGTTTATTGATCACCGTTGTGACGACCTACGAATTTACGAC
GCTTACGATCGATACGCCGTATATGTACATCGTGCTGATCTATGCGATTCGGGCGTTTGGGATGTCTATGCTGATGATGC
CGATTATGACTGCCGGCATGAACCAGCTTCCGCAGCATCTAAACAGCCACGGAACGGCGATGTCGAATACGCTTCGCCAA
ATCAGCGGTTCGATCGGAACGAGCTTGATCACGACGATTTATACGAATCGTACGACATTCCACTATGCATCAATGGCCGA
TCAGACAAATACGGCTGATCCGTCATTTATGAGCACATTGCAGTCAACGATTCAGTCAACCGCGCAATCGATGCATATAT
CTCTCGAACAAGCACAACAATATGTCGTGAAATATTTGACCGGACAGGCGCAGCTCAACTCGAACGTCATGGGTATTAAT
GACGCGTTTATGTGGGCGGCCGGGTTCTGTGTCATCGGTCTCGTTCTCAGCCTGTTTTTGAGGGATGTCAGAAAAGACAA
GCTGCGCAGGAAGAACAAAGCGCAGGAGCTGACATTGCTTCCGGCTCCTAAAGAAGCGAAAGAATCTTAA

Upstream 100 bases:

>100_bases
CAAAAACCCGTCTGACAAAGTGCTGCCGGGAATGAACGCATCCGTGAAAATTTCAAAATAACCGGGGGAAATAAAAACGA
GCGAAAGGAGGATGTCATAA

Downstream 100 bases:

>100_bases
AATGAGGAGGTTCTGACATGAAAATTTATGTTGTTTACGACAGTGAAGGCGAGCATACGAAAAAGCTGGCTGAAGCCATT
GCAGAAGGCGCGGGGGAACA

Product: YhcA

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MASQKKGNMSLLIILMAGLFLAILNQTLLNVAMPHLMTEFNVSATTIQWLTTGYMLVNGVLIPLSAFLIMRFGGRSLFLT
AMLLFTAGTLICGISPNFSTMLTGRLIQAVGGGILQPLVMTTILFIFPPESRGKGMGIFGLAMMFAPAVGPTLSGWVIEN
YSWRIMFYSLVPVGVIVIIFGFFLFKNMTEPKKIKLDTAGAILSVVGFAALLYGVSEAGSDGWDDPIVLSTVVIGVIGIA
AFIIQQLKSKEPMLDFRVFKYDMFSLSSVINAIITVALYAGMFLIPIYLQNLVGFTALQSGLLMLPGALVMLVMSPISGI
LFDKVGPRPLAIVGLLITVVTTYEFTTLTIDTPYMYIVLIYAIRAFGMSMLMMPIMTAGMNQLPQHLNSHGTAMSNTLRQ
ISGSIGTSLITTIYTNRTTFHYASMADQTNTADPSFMSTLQSTIQSTAQSMHISLEQAQQYVVKYLTGQAQLNSNVMGIN
DAFMWAAGFCVIGLVLSLFLRDVRKDKLRRKNKAQELTLLPAPKEAKES

Sequences:

>Translated_529_residues
MASQKKGNMSLLIILMAGLFLAILNQTLLNVAMPHLMTEFNVSATTIQWLTTGYMLVNGVLIPLSAFLIMRFGGRSLFLT
AMLLFTAGTLICGISPNFSTMLTGRLIQAVGGGILQPLVMTTILFIFPPESRGKGMGIFGLAMMFAPAVGPTLSGWVIEN
YSWRIMFYSLVPVGVIVIIFGFFLFKNMTEPKKIKLDTAGAILSVVGFAALLYGVSEAGSDGWDDPIVLSTVVIGVIGIA
AFIIQQLKSKEPMLDFRVFKYDMFSLSSVINAIITVALYAGMFLIPIYLQNLVGFTALQSGLLMLPGALVMLVMSPISGI
LFDKVGPRPLAIVGLLITVVTTYEFTTLTIDTPYMYIVLIYAIRAFGMSMLMMPIMTAGMNQLPQHLNSHGTAMSNTLRQ
ISGSIGTSLITTIYTNRTTFHYASMADQTNTADPSFMSTLQSTIQSTAQSMHISLEQAQQYVVKYLTGQAQLNSNVMGIN
DAFMWAAGFCVIGLVLSLFLRDVRKDKLRRKNKAQELTLLPAPKEAKES
>Mature_528_residues
ASQKKGNMSLLIILMAGLFLAILNQTLLNVAMPHLMTEFNVSATTIQWLTTGYMLVNGVLIPLSAFLIMRFGGRSLFLTA
MLLFTAGTLICGISPNFSTMLTGRLIQAVGGGILQPLVMTTILFIFPPESRGKGMGIFGLAMMFAPAVGPTLSGWVIENY
SWRIMFYSLVPVGVIVIIFGFFLFKNMTEPKKIKLDTAGAILSVVGFAALLYGVSEAGSDGWDDPIVLSTVVIGVIGIAA
FIIQQLKSKEPMLDFRVFKYDMFSLSSVINAIITVALYAGMFLIPIYLQNLVGFTALQSGLLMLPGALVMLVMSPISGIL
FDKVGPRPLAIVGLLITVVTTYEFTTLTIDTPYMYIVLIYAIRAFGMSMLMMPIMTAGMNQLPQHLNSHGTAMSNTLRQI
SGSIGTSLITTIYTNRTTFHYASMADQTNTADPSFMSTLQSTIQSTAQSMHISLEQAQQYVVKYLTGQAQLNSNVMGIND
AFMWAAGFCVIGLVLSLFLRDVRKDKLRRKNKAQELTLLPAPKEAKES

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=510, Percent_Identity=26.4705882352941, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1788710, Length=428, Percent_Identity=28.2710280373832, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI87081983, Length=409, Percent_Identity=25.6723716381418, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1790195, Length=440, Percent_Identity=26.3636363636364, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788392, Length=400, Percent_Identity=26.75, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87082231, Length=130, Percent_Identity=30, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323735, Length=324, Percent_Identity=25.3086419753086, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=225, Percent_Identity=27.5555555555556, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6322958, Length=338, Percent_Identity=23.0769230769231, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319770, Length=330, Percent_Identity=26.3636363636364, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57675; Mature: 57544

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.4 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASQKKGNMSLLIILMAGLFLAILNQTLLNVAMPHLMTEFNVSATTIQWLTTGYMLVNGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LIPLSAFLIMRFGGRSLFLTAMLLFTAGTLICGISPNFSTMLTGRLIQAVGGGILQPLVM
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHCCHHHHHHHH
TTILFIFPPESRGKGMGIFGLAMMFAPAVGPTLSGWVIENYSWRIMFYSLVPVGVIVIIF
HHHHHEECCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCEEEEHHHHHHHHHHHHHH
GFFLFKNMTEPKKIKLDTAGAILSVVGFAALLYGVSEAGSDGWDDPIVLSTVVIGVIGIA
HHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
AFIIQQLKSKEPMLDFRVFKYDMFSLSSVINAIITVALYAGMFLIPIYLQNLVGFTALQS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLLMLPGALVMLVMSPISGILFDKVGPRPLAIVGLLITVVTTYEFTTLTIDTPYMYIVLI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHH
YAIRAFGMSMLMMPIMTAGMNQLPQHLNSHGTAMSNTLRQISGSIGTSLITTIYTNRTTF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
HYASMADQTNTADPSFMSTLQSTIQSTAQSMHISLEQAQQYVVKYLTGQAQLNSNVMGIN
EEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCEECCC
DAFMWAAGFCVIGLVLSLFLRDVRKDKLRRKNKAQELTLLPAPKEAKES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC
>Mature Secondary Structure 
ASQKKGNMSLLIILMAGLFLAILNQTLLNVAMPHLMTEFNVSATTIQWLTTGYMLVNGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LIPLSAFLIMRFGGRSLFLTAMLLFTAGTLICGISPNFSTMLTGRLIQAVGGGILQPLVM
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHCCHHHHHHHH
TTILFIFPPESRGKGMGIFGLAMMFAPAVGPTLSGWVIENYSWRIMFYSLVPVGVIVIIF
HHHHHEECCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCEEEEHHHHHHHHHHHHHH
GFFLFKNMTEPKKIKLDTAGAILSVVGFAALLYGVSEAGSDGWDDPIVLSTVVIGVIGIA
HHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
AFIIQQLKSKEPMLDFRVFKYDMFSLSSVINAIITVALYAGMFLIPIYLQNLVGFTALQS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLLMLPGALVMLVMSPISGILFDKVGPRPLAIVGLLITVVTTYEFTTLTIDTPYMYIVLI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHH
YAIRAFGMSMLMMPIMTAGMNQLPQHLNSHGTAMSNTLRQISGSIGTSLITTIYTNRTTF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
HYASMADQTNTADPSFMSTLQSTIQSTAQSMHISLEQAQQYVVKYLTGQAQLNSNVMGIN
EEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCEECCC
DAFMWAAGFCVIGLVLSLFLRDVRKDKLRRKNKAQELTLLPAPKEAKES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]