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The map label for this gene is yfiE [H]

Identifier: 52784660

GI number: 52784660

Start: 881383

End: 882243

Strand: Direct

Name: yfiE [H]

Synonym: BLi00862

Alternate gene names: 52784660

Gene position: 881383-882243 (Clockwise)

Preceding gene: 52784659

Following gene: 52784661

Centisome position: 20.87

GC content: 49.36

Gene sequence:

>861_bases
ATGACGCAGCAAATCCACCCGGGCACTGAGCTCGGATATGTCAAGCTGAAGGTGAGCCGTCTTGAAAGATCGGTTTCTTT
TTACAGGGATGTGATCGGTTTTCAAGTATTAAAACAGGACGGCAAAACGGCGGAGTTCACAGTTGACGGAAAAAATCCGC
TTTTGATCATTGAGGAGATTCCAAATGCTGTTGTTCTTCCTGAACGATCGGCAGCAGGCCTTTATCATTTTGCCATTTTG
CTTCCAAACCGAAAGCAGCTCGGCATGGCTGTGAAGCATTTGATCCGCGCCGGCATTGAACTCGGCCAGGGAGATCACCT
TGTCAGCGAGGCGTTTTACTTGTCAGATCCTGATCAAAATGGAATCGAAATCTACCGCGACCGTCCGCGAGAGGGATGGA
AGCGCGATGAGCAGGGGAACTACGTGATGGGTACGGAGCCGGTTGATGTCCAAGGACTTCTGGAAGAAGCCGATGGCCAC
GAATGGCAAGGTCTTCCCCCGGAAACAAAAATCGGACACGTCCATTTTCACGTCCGGGATTTGAAGCAGGCGAAAGAATT
TTACCACTCTGTACTGGGATTTGATATCACAGGAGATTATCAACACATGTCAGCGCTTTTTGTCTCTGCCGGAGGCTACC
ATCATCATATCGGCTTAAACATCTGGGCCGGACAGCATGCGCCATTTGCCCCGCTTGATGCGGCAGGCATGGCGTATTAC
ACGGTTCTTTTACCGAATCGAGCGGAACTTGACCGAATTGCAAAAAAACTGAAAAGCTCGGGTTATGCCGTTGAGCATGA
AGAAAACGCATGGTTTGTTAAAGACCCGGCATCAGGTATTGATATCAGATTGACCGTTTGA

Upstream 100 bases:

>100_bases
GTTACGCTTCTTGCAATGTCTGTATACTTGGCCTTGAGCGGAAGCAGCATGCTGTCCCTTGGGGAAATGATCCTGAAATC
AAGGAAAGAAGGAAAAACAG

Downstream 100 bases:

>100_bases
AAAGGCGAAACTCCAGTTTCGCCTTTTTTTATGCCATTCAATCAAAGGACAACAAAAGCAAAGAATGAGAAGAAACAGAC
ATTAATAACAAAGATTCAGG

Product: YfiE

Products: NA

Alternate protein names: Catechol-induced ring cleavage extradiol dioxygenase [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MTQQIHPGTELGYVKLKVSRLERSVSFYRDVIGFQVLKQDGKTAEFTVDGKNPLLIIEEIPNAVVLPERSAAGLYHFAIL
LPNRKQLGMAVKHLIRAGIELGQGDHLVSEAFYLSDPDQNGIEIYRDRPREGWKRDEQGNYVMGTEPVDVQGLLEEADGH
EWQGLPPETKIGHVHFHVRDLKQAKEFYHSVLGFDITGDYQHMSALFVSAGGYHHHIGLNIWAGQHAPFAPLDAAGMAYY
TVLLPNRAELDRIAKKLKSSGYAVEHEENAWFVKDPASGIDIRLTV

Sequences:

>Translated_286_residues
MTQQIHPGTELGYVKLKVSRLERSVSFYRDVIGFQVLKQDGKTAEFTVDGKNPLLIIEEIPNAVVLPERSAAGLYHFAIL
LPNRKQLGMAVKHLIRAGIELGQGDHLVSEAFYLSDPDQNGIEIYRDRPREGWKRDEQGNYVMGTEPVDVQGLLEEADGH
EWQGLPPETKIGHVHFHVRDLKQAKEFYHSVLGFDITGDYQHMSALFVSAGGYHHHIGLNIWAGQHAPFAPLDAAGMAYY
TVLLPNRAELDRIAKKLKSSGYAVEHEENAWFVKDPASGIDIRLTV
>Mature_285_residues
TQQIHPGTELGYVKLKVSRLERSVSFYRDVIGFQVLKQDGKTAEFTVDGKNPLLIIEEIPNAVVLPERSAAGLYHFAILL
PNRKQLGMAVKHLIRAGIELGQGDHLVSEAFYLSDPDQNGIEIYRDRPREGWKRDEQGNYVMGTEPVDVQGLLEEADGHE
WQGLPPETKIGHVHFHVRDLKQAKEFYHSVLGFDITGDYQHMSALFVSAGGYHHHIGLNIWAGQHAPFAPLDAAGMAYYT
VLLPNRAELDRIAKKLKSSGYAVEHEENAWFVKDPASGIDIRLTV

Specific function: Involved in the meta cleavage of catechol to 2- hydroxymuconic semialdehyde. Essential for growth and viability in the presence of catechol and probably involved in the detoxification of catechol [H]

COG id: COG2514

COG function: function code R; Predicted ring-cleavage extradiol dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the extradiol ring-cleavage dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR000486 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =1.13.11.2 [H]

Molecular weight: Translated: 32073; Mature: 31942

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQIHPGTELGYVKLKVSRLERSVSFYRDVIGFQVLKQDGKTAEFTVDGKNPLLIIEEI
CCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEC
PNAVVLPERSAAGLYHFAILLPNRKQLGMAVKHLIRAGIELGQGDHLVSEAFYLSDPDQN
CCEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHEEEECCCCCC
GIEIYRDRPREGWKRDEQGNYVMGTEPVDVQGLLEEADGHEWQGLPPETKIGHVHFHVRD
CCCEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHH
LKQAKEFYHSVLGFDITGDYQHMSALFVSAGGYHHHIGLNIWAGQHAPFAPLDAAGMAYY
HHHHHHHHHHHHCCCCCCCHHHHHEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCCEEE
TVLLPNRAELDRIAKKLKSSGYAVEHEENAWFVKDPASGIDIRLTV
EEECCCHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCEEEEEEC
>Mature Secondary Structure 
TQQIHPGTELGYVKLKVSRLERSVSFYRDVIGFQVLKQDGKTAEFTVDGKNPLLIIEEI
CCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEC
PNAVVLPERSAAGLYHFAILLPNRKQLGMAVKHLIRAGIELGQGDHLVSEAFYLSDPDQN
CCEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHEEEECCCCCC
GIEIYRDRPREGWKRDEQGNYVMGTEPVDVQGLLEEADGHEWQGLPPETKIGHVHFHVRD
CCCEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHH
LKQAKEFYHSVLGFDITGDYQHMSALFVSAGGYHHHIGLNIWAGQHAPFAPLDAAGMAYY
HHHHHHHHHHHHCCCCCCCHHHHHEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCCEEE
TVLLPNRAELDRIAKKLKSSGYAVEHEENAWFVKDPASGIDIRLTV
EEECCCHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8704981; 9384377 [H]