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The map label for this gene is gmuB [H]

Identifier: 52784154

GI number: 52784154

Start: 307317

End: 307619

Strand: Reverse

Name: gmuB [H]

Synonym: BLi00332

Alternate gene names: 52784154

Gene position: 307619-307317 (Counterclockwise)

Preceding gene: 52784155

Following gene: 52784153

Centisome position: 7.28

GC content: 45.87

Gene sequence:

>303_bases
ATGAAGATCGCACTTGTATGTTCAGCCGGAATGTCTACGAGTATATTGGTAAAAAAAATGCGGGAAGAAGCGGAAAACCG
TTCATTGAAGGCGGAAATTAATGCGTATGCTGAGGCAGATTTGCCAAGCGATCTGGATGAAATTGATGTGATACTGATTG
GTCCGCAGGTCCGGTATTTAAAAGGGCAAATGACAGAGCGTGCCAAGCCGTATCATACACCCGTGGCCGTCATTGACCAA
ATGGCTTATGGAATGATGGACGGGGCAAAAGTGCTTAATCAAGCAATCAGCCTTGCAACCTGA

Upstream 100 bases:

>100_bases
TAATCCATGCTCAGGACCATTTAATGACAGCCATAACTGTAAAAGATTTGGCGAAAGAAATGATTGATTTATATCAAGAG
CTTGGGAAAGGAAGAGAAAA

Downstream 100 bases:

>100_bases
TGCTTTCCGCAAAGAACCTCGTTTCCTTTGAAAACGGGTTCTTTTTTATATTTGAAGTTTAAAAGAATGATTTTTTGAAA
GCGTTTGATTTTGTATAATA

Product: hypothetical protein

Products: NA

Alternate protein names: Glucomannan utilization protein B; PTS system oligo-beta-mannoside-specific EIIB component [H]

Number of amino acids: Translated: 100; Mature: 100

Protein sequence:

>100_residues
MKIALVCSAGMSTSILVKKMREEAENRSLKAEINAYAEADLPSDLDEIDVILIGPQVRYLKGQMTERAKPYHTPVAVIDQ
MAYGMMDGAKVLNQAISLAT

Sequences:

>Translated_100_residues
MKIALVCSAGMSTSILVKKMREEAENRSLKAEINAYAEADLPSDLDEIDVILIGPQVRYLKGQMTERAKPYHTPVAVIDQ
MAYGMMDGAKVLNQAISLAT
>Mature_100_residues
MKIALVCSAGMSTSILVKKMREEAENRSLKAEINAYAEADLPSDLDEIDVILIGPQVRYLKGQMTERAKPYHTPVAVIDQ
MAYGMMDGAKVLNQAISLAT

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1440

COG function: function code G; Phosphotransferase system cellobiose-specific component IIB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788034, Length=90, Percent_Identity=44.4444444444444, Blast_Score=69, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003501
- InterPro:   IPR013012 [H]

Pfam domain/function: PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 10943; Mature: 10943

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS51100 PTS_EIIB_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
7.0 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
7.0 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIALVCSAGMSTSILVKKMREEAENRSLKAEINAYAEADLPSDLDEIDVILIGPQVRYL
CEEEEEECCCCCHHHHHHHHHHHHHCCCEEHHHHHEECCCCCCCCCCEEEEEECCCHHHH
KGQMTERAKPYHTPVAVIDQMAYGMMDGAKVLNQAISLAT
HHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIALVCSAGMSTSILVKKMREEAENRSLKAEINAYAEADLPSDLDEIDVILIGPQVRYL
CEEEEEECCCCCHHHHHHHHHHHHHCCCEEHHHHHEECCCCCCCCCCEEEEEECCCHHHH
KGQMTERAKPYHTPVAVIDQMAYGMMDGAKVLNQAISLAT
HHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]