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The map label for this gene is citS [H]

Identifier: 52784051

GI number: 52784051

Start: 201818

End: 203434

Strand: Reverse

Name: citS [H]

Synonym: BLi00225

Alternate gene names: 52784051

Gene position: 203434-201818 (Counterclockwise)

Preceding gene: 52784052

Following gene: 52784050

Centisome position: 4.82

GC content: 45.52

Gene sequence:

>1617_bases
GTGCCTGTGGTCAAAAAACGGGTCAATTTTTCTCTGCAAACAAAAATTATGGGCTTGATTGCAGCACTGCTTATCTTAGT
AATCGGCTTGCTGACAGTCACGTTTGCCGTTCAGCAAACAAAAGAAGGAAGAAAACAGTCCGAACAGCTTGCCGTTCAGA
CTGCAAAAACCATTTCTTATATGCCTCCGGTAAAAGAGCTCTTTCAAAAGAAGCGCGGGCATAAGGGAGAGGCGCAAGAG
GTCATTGAACAAATGAAAGAACAGACCGGTGCCTATACTATTTATGTCCTCAACGAAAAGGGAGAGATCAAAATCGCCTC
GGAAAAGAGAGGTCAAACGAAGCTGACAAGAAGCAGAAAAATCTTGTTCGGCGGCTCTCACATCTCTAAAACAAAAAACG
ATGGCCGCATGGTGATCAGAGGAAGTGCGCCGATTATCCAGGAACAAAGTGGTTACAGCAAGGTCATCGGCAGTGTGTCT
GTTGATTTTCTGTTAAACGAGACAGAACAACGTATTAAAGAGAATGTGAGAAAATTGAGCTTCATGGCTGTTCTCGTTTT
GCTCATAGGATTTGCGGGTGCCGCCGCTCTTGCCAAAAGCATTAGAAAAGATACGCTTGGCCTTGAACCCCAAGAGATTG
CATCTCTTTACCGTGAACGAAATGCGATGCTGCGTGCGATTAAAGAAGGGATCATTGCCACCAACCGTGAAGGAGTCGTC
ACAATGATGAACGTATCGGCAGCGGAAATGCTTAGGCTTCCCGAACCTGTGGTCAACCGGCCAATTGATGACATCCTGCC
CGGAGCCGGAATAATGTCTGTCCTTCAGCAGCGGCAGATGTTGCTGAATCAGGAGGTCTGCGTCAACGATCAAGTGTTTA
TTTTAAATACAAAAATGATGCTCCAAAACGGTCAAACTTACGGAATTGTCGTAAGTTTCCGTCAAAAAACCGAGTTAAAA
AGGCTCATTGACACGCTGACAGAGGTGCAAAAATACTCTGAAGATCTCCGCGCGCAAACACATGAATTCTCAAATAAGCT
TTATGTGATCCTAGGCCTGCTTGAGCTAGGTGAGTATGAAGCAGCGATCGACCTGATTAAAGAGGAATACGCCATTCAAA
ATGAACAGCACGACATTTTATTCAAAAACATTCATTCTCAGCAGGTGCAGGCTATCCTGTTGGGCAAAATGGGAAAGGCT
TCGGAGAAAAAAGTTAAGTTGTCCATTGATGAAAACAGCACGCTTGAGCCGCTTCCTGAGCATATCGGGATGTCTCATCT
CATTACGATCATCGGGAATTTAATTGATAACGCTTTCGAAGCCGTAGCTGAGCAAAATGTGCGGCTTGTTTCATTTTTCA
TCACTGACATCGGCCGCGATATTGTCATAGAAGTGGCTGACACAGGATGCGGCGTAGCGCCTGATAAGATGAAAGCCATC
TTTGAACGAGGATATTCTTCAAAGGGGATGAAGAGAGGCTACGGGCTGGCCAACGTAAAGGAATCTGTACGTGAACTTGG
CGGTTGGATTGAAGTGGCCAATCAAAAAGGCGGCGGAGCGGTATTTACCGTTTTTATACCGAAGGAGAAGCAAACACGAG
AGGGGGAATCCATTTGA

Upstream 100 bases:

>100_bases
GATATACTTGGGCTTTTTCGTTATCTTGCAAAGGATATTTTTAAAAATGCCAAAATTTCCGAATAAAAAGTGGTATGATT
TTTGGTAGAAAGGACGGCTG

Downstream 100 bases:

>100_bases
TTGATATCGTGATCGCGGAGGATGATTTCCGTGTAGCGCAAATTCATGAAAGATTGATCGAACAGCTTGATGGATTCAAC
ATTATCGGTAAAGCGGCCAA

Product: CitS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MPVVKKRVNFSLQTKIMGLIAALLILVIGLLTVTFAVQQTKEGRKQSEQLAVQTAKTISYMPPVKELFQKKRGHKGEAQE
VIEQMKEQTGAYTIYVLNEKGEIKIASEKRGQTKLTRSRKILFGGSHISKTKNDGRMVIRGSAPIIQEQSGYSKVIGSVS
VDFLLNETEQRIKENVRKLSFMAVLVLLIGFAGAAALAKSIRKDTLGLEPQEIASLYRERNAMLRAIKEGIIATNREGVV
TMMNVSAAEMLRLPEPVVNRPIDDILPGAGIMSVLQQRQMLLNQEVCVNDQVFILNTKMMLQNGQTYGIVVSFRQKTELK
RLIDTLTEVQKYSEDLRAQTHEFSNKLYVILGLLELGEYEAAIDLIKEEYAIQNEQHDILFKNIHSQQVQAILLGKMGKA
SEKKVKLSIDENSTLEPLPEHIGMSHLITIIGNLIDNAFEAVAEQNVRLVSFFITDIGRDIVIEVADTGCGVAPDKMKAI
FERGYSSKGMKRGYGLANVKESVRELGGWIEVANQKGGGAVFTVFIPKEKQTREGESI

Sequences:

>Translated_538_residues
MPVVKKRVNFSLQTKIMGLIAALLILVIGLLTVTFAVQQTKEGRKQSEQLAVQTAKTISYMPPVKELFQKKRGHKGEAQE
VIEQMKEQTGAYTIYVLNEKGEIKIASEKRGQTKLTRSRKILFGGSHISKTKNDGRMVIRGSAPIIQEQSGYSKVIGSVS
VDFLLNETEQRIKENVRKLSFMAVLVLLIGFAGAAALAKSIRKDTLGLEPQEIASLYRERNAMLRAIKEGIIATNREGVV
TMMNVSAAEMLRLPEPVVNRPIDDILPGAGIMSVLQQRQMLLNQEVCVNDQVFILNTKMMLQNGQTYGIVVSFRQKTELK
RLIDTLTEVQKYSEDLRAQTHEFSNKLYVILGLLELGEYEAAIDLIKEEYAIQNEQHDILFKNIHSQQVQAILLGKMGKA
SEKKVKLSIDENSTLEPLPEHIGMSHLITIIGNLIDNAFEAVAEQNVRLVSFFITDIGRDIVIEVADTGCGVAPDKMKAI
FERGYSSKGMKRGYGLANVKESVRELGGWIEVANQKGGGAVFTVFIPKEKQTREGESI
>Mature_537_residues
PVVKKRVNFSLQTKIMGLIAALLILVIGLLTVTFAVQQTKEGRKQSEQLAVQTAKTISYMPPVKELFQKKRGHKGEAQEV
IEQMKEQTGAYTIYVLNEKGEIKIASEKRGQTKLTRSRKILFGGSHISKTKNDGRMVIRGSAPIIQEQSGYSKVIGSVSV
DFLLNETEQRIKENVRKLSFMAVLVLLIGFAGAAALAKSIRKDTLGLEPQEIASLYRERNAMLRAIKEGIIATNREGVVT
MMNVSAAEMLRLPEPVVNRPIDDILPGAGIMSVLQQRQMLLNQEVCVNDQVFILNTKMMLQNGQTYGIVVSFRQKTELKR
LIDTLTEVQKYSEDLRAQTHEFSNKLYVILGLLELGEYEAAIDLIKEEYAIQNEQHDILFKNIHSQQVQAILLGKMGKAS
EKKVKLSIDENSTLEPLPEHIGMSHLITIIGNLIDNAFEAVAEQNVRLVSFFITDIGRDIVIEVADTGCGVAPDKMKAIF
ERGYSSKGMKRGYGLANVKESVRELGGWIEVANQKGGGAVFTVFIPKEKQTREGESI

Specific function: Member of the two-component regulatory system CitT/CitS. Regulates the expression of the citM-yflN operon. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=529, Percent_Identity=29.3005671077505, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1786837, Length=539, Percent_Identity=29.1280148423006, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI1790436, Length=129, Percent_Identity=34.1085271317829, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59892; Mature: 59761

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVVKKRVNFSLQTKIMGLIAALLILVIGLLTVTFAVQQTKEGRKQSEQLAVQTAKTISY
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
MPPVKELFQKKRGHKGEAQEVIEQMKEQTGAYTIYVLNEKGEIKIASEKRGQTKLTRSRK
CCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCCCHHHHHCCE
ILFGGSHISKTKNDGRMVIRGSAPIIQEQSGYSKVIGSVSVDFLLNETEQRIKENVRKLS
EEECCCCCCCCCCCCEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMAVLVLLIGFAGAAALAKSIRKDTLGLEPQEIASLYRERNAMLRAIKEGIIATNREGVV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
TMMNVSAAEMLRLPEPVVNRPIDDILPGAGIMSVLQQRQMLLNQEVCVNDQVFILNTKMM
EEECCCHHHHHHCCCHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHH
LQNGQTYGIVVSFRQKTELKRLIDTLTEVQKYSEDLRAQTHEFSNKLYVILGLLELGEYE
HHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCCHH
AAIDLIKEEYAIQNEQHDILFKNIHSQQVQAILLGKMGKASEKKVKLSIDENSTLEPLPE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHH
HIGMSHLITIIGNLIDNAFEAVAEQNVRLVSFFITDIGRDIVIEVADTGCGVAPDKMKAI
HHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHH
FERGYSSKGMKRGYGLANVKESVRELGGWIEVANQKGGGAVFTVFIPKEKQTREGESI
HHCCCCCCCCCCCCCHHHHHHHHHHHCCHHEECCCCCCCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
PVVKKRVNFSLQTKIMGLIAALLILVIGLLTVTFAVQQTKEGRKQSEQLAVQTAKTISY
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
MPPVKELFQKKRGHKGEAQEVIEQMKEQTGAYTIYVLNEKGEIKIASEKRGQTKLTRSRK
CCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCCCHHHHHCCE
ILFGGSHISKTKNDGRMVIRGSAPIIQEQSGYSKVIGSVSVDFLLNETEQRIKENVRKLS
EEECCCCCCCCCCCCEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMAVLVLLIGFAGAAALAKSIRKDTLGLEPQEIASLYRERNAMLRAIKEGIIATNREGVV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
TMMNVSAAEMLRLPEPVVNRPIDDILPGAGIMSVLQQRQMLLNQEVCVNDQVFILNTKMM
EEECCCHHHHHHCCCHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHH
LQNGQTYGIVVSFRQKTELKRLIDTLTEVQKYSEDLRAQTHEFSNKLYVILGLLELGEYE
HHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCCHH
AAIDLIKEEYAIQNEQHDILFKNIHSQQVQAILLGKMGKASEKKVKLSIDENSTLEPLPE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHH
HIGMSHLITIIGNLIDNAFEAVAEQNVRLVSFFITDIGRDIVIEVADTGCGVAPDKMKAI
HHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHH
FERGYSSKGMKRGYGLANVKESVRELGGWIEVANQKGGGAVFTVFIPKEKQTREGESI
HHCCCCCCCCCCCCCHHHHHHHHHHHCCHHEECCCCCCCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 10972810 [H]