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The map label for this gene is sigH

Identifier: 52783954

GI number: 52783954

Start: 115357

End: 116028

Strand: Direct

Name: sigH

Synonym: BLi00116

Alternate gene names: 52783954

Gene position: 115357-116028 (Clockwise)

Preceding gene: 52783953

Following gene: 52783955

Centisome position: 2.73

GC content: 42.71

Gene sequence:

>672_bases
GTGAACTTACAAAACAACCAGGGAAAATTCAGCAAAGAACAGTTCAGCAAAGAACGTTTTTGCCAGTTGGAAGACGAGCA
GGTCATTGAAATGGTTCATGTCGGAGACAGTGATGCGTTAGATTACTTGATTACAAAGTACCGTAATTTTGTAAGGGCAA
AAGCAAGATCCTACTTCTTAATCGGGGCTGATCGGGAAGATATCGTTCAGGAAGGCATGATTGGACTCTACAAATCTATC
CGTGATTTTAGAGAGGACAAGCTGACTTCATTCAAAGCTTTTGCAGAATTATGCATTACCCGCCAAATTATTACCGCTAT
CAAAACAGCTACTCGCCAGAAACATATTCCGCTCAATTCTTATGTGTCGCTGGACAAGCCCATTTATGACGAGGAATCAG
ACCGGACGCTTCTCGACGTGATTTCAGGGGCCAAGGTCATGAATCCTGAAGAGCTGATCATCAATCAGGAAGAATTCGAT
GATATCGAGCTGAAAATGGGAGAATTGCTTAGTGATTTAGAAAGAAAAGTTCTTGCGCTCTATCTCGACGGAAGATCCTA
TCAGGAAATATCCGAAGAATTAAACCGCCATGTAAAATCGATCGATAATGCGCTCCAGCGTGTCAAAAGGAAGCTTGAAA
AGTACCTGGAGCTCCGCGAAATCAGCTTGTAG

Upstream 100 bases:

>100_bases
GATCTTCGAAAAATGGCGGCGTGGCGACTTGGATTAGGTTGACGCTTTTTTGTCCATTACTGTATAATATTTCTATCTAC
GTGCGGTCGGGGGGATCGGA

Downstream 100 bases:

>100_bases
CCGATGCTTATGGCATATTGACAGTCATTTTATGACTGTGATATGTTACTTAGGAAATGAACGTCCAGAAAAAGGTGTAA
ACATGAAGAAAAAAGTAACG

Product: RNA polymerase factor sigma-70

Products: NA

Alternate protein names: Sigma-30

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MNLQNNQGKFSKEQFSKERFCQLEDEQVIEMVHVGDSDALDYLITKYRNFVRAKARSYFLIGADREDIVQEGMIGLYKSI
RDFREDKLTSFKAFAELCITRQIITAIKTATRQKHIPLNSYVSLDKPIYDEESDRTLLDVISGAKVMNPEELIINQEEFD
DIELKMGELLSDLERKVLALYLDGRSYQEISEELNRHVKSIDNALQRVKRKLEKYLELREISL

Sequences:

>Translated_223_residues
MNLQNNQGKFSKEQFSKERFCQLEDEQVIEMVHVGDSDALDYLITKYRNFVRAKARSYFLIGADREDIVQEGMIGLYKSI
RDFREDKLTSFKAFAELCITRQIITAIKTATRQKHIPLNSYVSLDKPIYDEESDRTLLDVISGAKVMNPEELIINQEEFD
DIELKMGELLSDLERKVLALYLDGRSYQEISEELNRHVKSIDNALQRVKRKLEKYLELREISL
>Mature_223_residues
MNLQNNQGKFSKEQFSKERFCQLEDEQVIEMVHVGDSDALDYLITKYRNFVRAKARSYFLIGADREDIVQEGMIGLYKSI
RDFREDKLTSFKAFAELCITRQIITAIKTATRQKHIPLNSYVSLDKPIYDEESDRTLLDVISGAKVMNPEELIINQEEFD
DIELKMGELLSDLERKVLALYLDGRSYQEISEELNRHVKSIDNALQRVKRKLEKYLELREISL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in the transition to post-exponential phase in the beginning of sporulation

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family

Homologues:

None

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): RPSH_BACLI (P02964)

Other databases:

- EMBL:   M29694
- EMBL:   X00413
- PIR:   B28625
- ProteinModelPortal:   P02964
- GO:   GO:0005622
- InterPro:   IPR014284
- InterPro:   IPR014218
- InterPro:   IPR016371
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR000792
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PIRSF:   PIRSF002939
- TIGRFAMs:   TIGR02937
- TIGRFAMs:   TIGR02859

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 26109; Mature: 26109

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00715 SIGMA70_1; PS00716 SIGMA70_2; PS00622 HTH_LUXR_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLQNNQGKFSKEQFSKERFCQLEDEQVIEMVHVGDSDALDYLITKYRNFVRAKARSYFL
CCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEE
IGADREDIVQEGMIGLYKSIRDFREDKLTSFKAFAELCITRQIITAIKTATRQKHIPLNS
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
YVSLDKPIYDEESDRTLLDVISGAKVMNPEELIINQEEFDDIELKMGELLSDLERKVLAL
HHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHH
YLDGRSYQEISEELNRHVKSIDNALQRVKRKLEKYLELREISL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLQNNQGKFSKEQFSKERFCQLEDEQVIEMVHVGDSDALDYLITKYRNFVRAKARSYFL
CCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEE
IGADREDIVQEGMIGLYKSIRDFREDKLTSFKAFAELCITRQIITAIKTATRQKHIPLNS
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
YVSLDKPIYDEESDRTLLDVISGAKVMNPEELIINQEEFDDIELKMGELLSDLERKVLAL
HHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHH
YLDGRSYQEISEELNRHVKSIDNALQRVKRKLEKYLELREISL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3277943; 6322121